scholarly journals Neutralization of the inhibitory effect of sodium polyanetholesulfonate on Neisseria meningitidis in blood cultures processed with the Du Pont Isolator System.

1984 ◽  
Vol 20 (1) ◽  
pp. 40-42 ◽  
Author(s):  
R K Scribner ◽  
D F Welch
Keyword(s):  
Neisseria Meningitidis ◽  
Inhibitory Effect ◽  
Blood Cultures

scholarly journals Effects of two blood culture anticoagulants on growth of Neisseria meningitidis

1978 ◽  
Vol 7 (4) ◽  
pp. 332-336
Author(s):  
L Rintala ◽  
H M Pollock
Keyword(s):  
Blood Culture ◽  
Neisseria Meningitidis ◽  
Yeast Extract ◽  
Brain Heart Infusion ◽  
Inhibitory Effect ◽  
Inoculum Size ◽  
Blood Cultures ◽  
Artificial Blood

Tests of 25 strains of Neisseria meningitidis for sensitivity to sodium polyanethol sulfonate (SPS) showed that the sensitivity of strains varied with both inoculum size and SPS concentration. In Trypticase soy broth (TSB), 2 out of 13 strains were sensitive to 0.05% SPS, whereas 8 out of 13 strains were sensitive to the same concentration of SPS in brain heart infusion (BHI). In artificial blood cultures with six strains of meningococci, the addition of 10% defibrinated blood was found to eliminate the sensitivity of all six strains to SPS in BHI, but not of the two strains in TSB. Addition of 1.2% gelatin to artificial blood cultures eliminated the inhibitory effect of 0.05% SPS, whereas the addition of 1% yeast extract to blood cultures containing 0.025% or 0.05% SPS enhanced the inhibitory effect of this anticoagulant. None of the 13 strains tested was inhibited by 0.05% sodium amylosulfate in TSB or BHI alone or in artificial blood cultures with these media.

scholarly journals Gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Neisseria meningitidis in blood culture media

1977 ◽  
Vol 6 (1) ◽  
pp. 1-3 ◽  
Author(s):  
J Eng ◽  
E Holten
Keyword(s):  
Blood Culture ◽  
Neisseria Meningitidis ◽  
Growth Medium ◽  
Solid Medium ◽  
Culture Media ◽  
Inhibitory Effect ◽  
Blood Cultures ◽  
Nutrient Broth

The inhibitory effect of sodium polyanethol sulfonate (0.05%) upon growth of Neisseria meningitidis was found to be neutralized by adding gelatin (l.1%) to the growth medium. The neutralizing effect was demonstrated in solid medium, as well as in nutrient broth for blood cultures. The findings parallel those of Wilkins and West (6) regarding gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Peptostreptococcus anaerobius.

scholarly journals Prostaglandin-E2 is a potent inhibitor of human interleukin 12 production.

1995 ◽  
Vol 181 (2) ◽  
pp. 775-779 ◽  
Author(s):  
T C van der Pouw Kraan ◽  
L C Boeije ◽  
R J Smeenk ◽  
J Wijdenes ◽  
L A Aarden
Keyword(s):  
Human Immunodeficiency Virus ◽  
Prostaglandin E2 ◽  
Potent Inhibitor ◽  
Allergic Diseases ◽  
Inhibitory Effect ◽  
Blood Cultures ◽  
Interleukin 12 ◽  
T Helper ◽  
Th2 Response ◽  
Immunodeficiency Virus

During human immunodeficiency virus infection and allergic diseases, characterized by a dominant T helper (Th) 2 response, overproduction of prostaglandin E2 (PGE2) is observed. In this paper we studied the effect of PGE2 on interleukin (IL)-12 synthesis, because this cytokine has been described to be essential in induction of Th1 responses. IL-12 synthesis was induced in monocytes that were stimulated with Neisseria meningitidis-derived lipopolysaccharide in whole blood cultures. PGE2 almost completely inhibited lipopolysaccharide induced IL-12 production, whereas IL-6 production was only partially inhibited by PGE2. In contrast, the production of IL-10 was approximately twofold enhanced at these conditions. The effects of PGE2 were due to its cAMP-inducing capacity, since they could be mimicked by other cAMP inducers. Recombinant human IL-10 also inhibited IL-12 and IL-6 production. However, the inhibitory effect of PGE2 on IL-12 production was independent of IL-10 since neutralizing anti-IL-10 antibodies were unable to reverse this inhibition. These results suggest that the capacity of an antigen to induce PGE2 synthesis may play a crucial role in the development of either a Th1 or Th2 response.

Neisseria meningitidis Bacteremia in Children: Quantitation of Bacteremia and Spontaneous Clinical Recovery Without Antibiotic Therapy

PEDIATRICS ◽  
1987 ◽  
Vol 80 (1) ◽  
pp. 63-67 ◽  
Author(s):  
T. Dennis Sullivan ◽  
Leonard J. LaScolea
Keyword(s):  
Blood Culture ◽  
Antibiotic Therapy ◽  
Neisseria Meningitidis ◽  
Invasive Disease ◽  
Blood Cultures ◽  
Negative Blood Culture ◽  
Clinical Recovery ◽  
Oral Amoxicillin ◽  
Occult Bacteremia ◽  
The Relationship

The relationship between the magnitude of bacteremia due to Neisseria meningitidis and the clinical diagnosis was determined for 43 children who had fever in the presence or absence of focal signs of infection. Bacteremia was quantitated by the previously described procedure using heparinized blood (0.2 to 1.0 mL). Additionally, blood was cultured by means of the radiometric Bactec technique. Seventeen patients had meningitis, 12 had meningococcemia, 13 had unsuspected or "occult" bacteremia, and five had other diagnoses. "Occult" bactermia was diagnosed initially in four patients, but subsequently meningitis was diagnosed. All 13 patients with 500 or more organisms per milliliter had meningitis or meningococcemia in contrast to 12 (55%) of 22 patients with less than 500 organisms per milliliter (P ≤ .0035). Only 18 (42%) of these patients bacteremic with N meningitidis presented with petechiae or purpura. All 13 children with occult bacteremia were sent home after blood cultures were obtained; six of the 13 received a regimen of oral amoxicillin for otitis media. At reexamination (interval 16 to 119 hours) four had meningitis, seven were clinically improved (afebrile, negative blood culture, without invasive disease), and two were still mildly febrile with negative blood culture. Three of these bacteremic children experienced spontaneous clinical and bacteriologic resolution without antibiotic treatment. This has not been previously reported.

scholarly journals Nasal secretions of Neisseria lactamica carriers have an inhibitory effect on Neisseria meningitidis attachment to human oroepithelial cells

1986 ◽  
Vol 81 (4) ◽  
pp. 453-457 ◽  
Author(s):  
João Ramos Costa Andrade ◽  
Margareth da Cunha Marques ◽  
Milden Rodrigues de Santa Rosa
Keyword(s):  
Neisseria Meningitidis ◽  
Inhibitory Effect ◽  
Bacterial Adherence ◽  
Inhibiting Activity ◽  
Neisseria Lactamica ◽  
Nasal Secretions ◽  
Strong Attachment

Nasal secretions of volunteers colonized by N. lactamica impaired the attachment of N. lactamica and of meningococci of groups A and B to oroepithelial cells. Bacterial adherence was found to be mediated by nonpiliated adhesins with antigen(s) which probably are shared by the strains tested. Although a strong attachment-inhibiting activity arises in their nasal secretions, volunteers remained colonized by N. lactamica. This evidence suggest that the eradication of Neisseria carriage is a multifactorial event.

Mouse models of infection for Neisseria meningitidis B,2b and Haemophilus influenzae type b diseases

1986 ◽  
Vol 32 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Bernard R. Brodeur ◽  
Peggy S. Tsang ◽  
Josée Hamel ◽  
Yolande Larose ◽  
Serge Montplaisir
Keyword(s):  
Haemophilus Influenzae ◽  
Neisseria Meningitidis ◽  
Inbred Mouse ◽  
Blood Cultures ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Bacterial Strains ◽  
Enhancement Factors ◽  
C57bl Mice

A disseminated and fatal infection was established in C57BL mice, injected intraperitoneally with either Neisseria meningitidis B,2b or Haemophilus influenzae type b bacteria plus enhancement factors. The effects of mucin, hemoglobin, and iron dextran as enhancement of bacterial infectivity in mice were evaluated individually and in combination. A mixture of mucin and hemoglobin was most effective in enhancing the virulence of the pathogens. Inbred mouse lines were more susceptible than outbred ones. Relative virulence of a number of bacterial strains was also compared in one selected mouse line. Neisseria meningitidis B,2b and Haemophilus influenzae type b strains were more virulent than non-B,2b and nontypable strains. Finally, the course of bacteremia for the two infections in mice was followed by quantitative blood cultures. The animals succumbed to the generalized condition within 72 h. In the case of Neisseria meningitidis B,2b, 10 organisms with 4% mucin and 1.6% hemoglobin were sufficient to kill 50% of the animals. For Haemophilus influenzae type b, 300 bacteria with 5% mucin and 2% hemoglobin were necessary to obtain similar effects.

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