Electroporation of Synthetic DNA Antigens Offers Protection in Nonhuman Primates Challenged with Highly Pathogenic Avian Influenza Virus

2009 ◽
Vol 83(9)
pp. 4624-4630
Dominick J. Laddy
Jian Yan
Amir S. Khan
Hanne Andersen
Amanda Cohn
Jack Greenhouse
Mark Lewis
Jody Manischewitz
Lisa R. King
Hana Golding
Ruxandra Draghia-Akli
David B. Weiner

ABSTRACT Avian influenza highlights the need for novel vaccination techniques that would allow for the rapid design and production of safe and effective vaccines. An ideal platform would be capable of inducing both protective antibodies and potent cellular immune responses. These potential advantages of DNA vaccines remain unrealized due to a lack of efficacy in large animal studies and in human trials. Questions remain regarding the potential utility of cellular immune responses against influenza virus in primates. In this study, by construct optimization and in vivo electroporation of synthetic DNA-encoded antigens, we observed the induction of cross-reactive cellular and humoral immune responses individually capable of providing protection from influenza virus infection in the rhesus macaque. These studies advance the DNA vaccine field and provide a novel, more tolerable vaccine with broad immunogenicity to avian influenza virus. This approach appears important for further investigation, including studies with humans.

Anthony T. DiPiazza
Katherine A. Richards
Wen-Chun Liu
Randy A. Albrecht
Andrea J. Sant

2010 ◽
Vol 17(4)
pp. 683-687
Abbas Jamali
Farzaneh Sabahi
Taravat Bamdad
Hamidreza Hashemi
Fereidoun Mahboudi
Masume Tavasoti Kheiri

2003 ◽
Vol 94(1-2)
pp. 47-62
G Soboll
D.W Horohov
B.M Aldridge
C.W Olsen
M.W McGregor
R.J Drape
M.D Macklin
W.F Swain
D.P Lunn

2020 ◽
Vol 12(12)
pp. 1396
Xiaoli Hao
Shuai Li
Lina Chen
Maoli Dong
Jiongjiong Wang
Jiao Hu
Min Gu
Xiaoquan Wang
Shunlin Hu
Daxin Peng
Xiufan Liu
Shaobin Shang

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