scholarly journals Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene.

1986 ◽  
Vol 6 (3) ◽  
pp. 842-848 ◽  
Author(s):  
J D McDonald ◽  
F K Lin ◽  
E Goldwasser

The gene for mouse erythropoietin was cloned and sequenced. We present here a preliminary analysis of the overall genomic organization of the coding portions and the two flanking regions of the gene. This is the third mammalian erythropoietin for which the sequence is available, but it represents the first from a nonprimate species. We investigated the evolutionary divergence of sequence and structure of the three erythropoietins and identified specific regions of the molecules that are apparently under various degrees, and perhaps different types, of functional constraint.

1986 ◽  
Vol 6 (3) ◽  
pp. 842-848
Author(s):  
J D McDonald ◽  
F K Lin ◽  
E Goldwasser

The gene for mouse erythropoietin was cloned and sequenced. We present here a preliminary analysis of the overall genomic organization of the coding portions and the two flanking regions of the gene. This is the third mammalian erythropoietin for which the sequence is available, but it represents the first from a nonprimate species. We investigated the evolutionary divergence of sequence and structure of the three erythropoietins and identified specific regions of the molecules that are apparently under various degrees, and perhaps different types, of functional constraint.


2013 ◽  
Vol 54 (3) ◽  
pp. 289-299
Author(s):  
Jürgen Hunkemöller

The recognition of topoi, i.e. traditional formulae, is an important means of musical analysis. To illustrate this, the paper discusses the types of the battaglia and the pastoral in Bach’s Cantata Halt im Gedächtnis Jesum Christ, and briefly enumerates different types of allusions to jazz in 20th-century compositions by Stravinsky, Milhaud, Blacher, Tippet, and Zimmermann. Then it raises the possibility of an analysis of topoi in Bartók’s music in four main categories. It considers Bartók’s musical quotations from Bach to Shostakovich; the chorale as special topos appearing in Mikrokosmos, in the Concerto for Orchestra, in the Adagio religioso of the Third Piano Concerto; the topos-like employment of the tritone; and finally the idea of a Bartókian Arcadia in the Finale of Music for Strings, and the integration of bird song in the Adagio religioso.


Minerals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 235
Author(s):  
Nicolò Maria Ippolito ◽  
Ionela Birloaga ◽  
Francesco Ferella ◽  
Marcello Centofanti ◽  
Francesco Vegliò

The present paper is focused on the extraction of gold from high-grade e-waste, i.e., spent electronic connectors and plates, by leaching and electrowinning. These connectors are usually made up of an alloy covered by a layer of gold; sometimes, in some of them, a plastic part is also present. The applied leaching system consisted of an acid solution of diluted sulfuric acid (0.2 mol/L) with thiourea (20 g/L) as a reagent and ferric sulfate (21.8 g/L) as an oxidant. This system was applied on three different high-grade e-waste, namely: (1) Connectors with the partial gold-plated surface (Au concentration—1139 mg/kg); (2) different types of connectors with some of which with completely gold-plated surface (Au concentration—590 mg/kg); and (3) connectors and plates with the completely gold-plated surface (Au concentration—7900 mg/kg). Gold dissolution yields of 52, 94, and 49% were achieved from the first, second, and third samples, respectively. About 95% of Au recovery was achieved after 1.5 h of electrowinning at a current efficiency of only 4.06% and current consumption of 3.02 kWh/kg of Au from the leach solution of the third sample.


Zootaxa ◽  
2021 ◽  
Vol 4999 (2) ◽  
Author(s):  
KLAUDIA FLORCZYK ◽  
CHRISTER FÅHRAEUS ◽  
PIERRE BOYER ◽  
ANNA ZUBEK ◽  
TOMASZ W. PYRCZ

A new, and only the third known species of the Neotropical montane genus Oressinoma Doubleday is described—O. sorina n. sp., from the Andes of central Peru. It is distinguishable immediately from the other two congeners by the shape of the hindwing underside submarginal orange band, and by the male genitalia. The systematics of Oressinoma are reviewed. A preliminary analysis is carried out based on COI barcode confirming the separate specific status of O. sorina n. sp. in relation to other two congeners. Both barcode and genital morphology data suggest that the widespread O. typhla Doubleday may be a complex of allopatric or, locally parapatric species. The genus Oressinoma is the only neotropical member of the predominantly Australian subtribe Coenonymphina, represented in the entire Holarctic by one genus only—Coenonympha Hübner, considered as the putative sister-genus of Oressinoma. Their origins and relationships are briefly discussed.


2013 ◽  
Vol 3 (3) ◽  
pp. 1-66
Author(s):  
Inge Pasteels ◽  
Dimitri Mortelmans

In deze bijdrage wordt het herpartneren na een scheiding uit een eerste huwelijk beschreven. Met gegevens van “Scheiding in Vlaanderen” verzameld bij 1192 mannen en 1448 vrouwen gescheiden tussen 1981 en 2005, wordt vooreerst de timing van het herpartneren alsook de leefvorm waarvoor men kiest in postmaritale relaties, bestudeerd. We onderscheiden LAT‐relaties, ongehuwd samenwoonrelaties en tweede huwelijken. Daarna wordt de huidige partnerstatus van gescheidenen anno 2010 in kaart gebracht. Ten derde komt de mate waarin partners uit eerste huwelijken of postmaritale relaties gelijkend zijn aan bod. Vervolgens wordt de stabiliteit van LAT‐relaties of ongehuwde samenwoonrelaties na een eerste huwelijk bestudeerd. Een beschrijving van postmaritale relatietrajecten met aandacht voor relatierang en relatietype rondt deze bijdrage af. Alle analyses worden apart uitgevoerd voor mannen en vrouwen van verschillende scheidingscohorten. Abstract : Using surveydata of “Divorce in Flanders” for 1192 men and 1448 women, divorced between 1981 and 2005, we explore repartnering after a first divorce. Firstly, prevalence and timing of repartnering are examined. LAT‐relationships, unmarried cohabitation and remarriage are considered as different types of repartnering. Secondly, the current partner status for divorcees in 2010 is described. Homogamy in first marriages and in higher order relationships is the third topic in this article. At last, stability of postmarital relationships and partnership trajectories are explored. Data are analyzed using a cohort perspective and a gender perspective.


2002 ◽  
Vol 282 (5) ◽  
pp. F898-F909 ◽  
Author(s):  
Christie P. Thomas ◽  
Randy W. Loftus ◽  
Kang Z. Liu ◽  
Omar A. Itani

The mRNA for the β-subunit of the epithelial Na+ channel (β-ENaC) is regulated developmentally and, in some tissues, in response to corticosteroids. To understand the mechanisms of transcriptional regulation of the human β-ENaC gene, we characterized the 5′ end of the gene and its 5′-flanking regions. Adaptor-ligated human kidney and lung cDNA were amplified by 5′ rapid amplification of cDNA ends, and transcription start sites of two 5′ variant transcripts were determined by nuclease protection or primer extension assays. Cosmid clones that contain the 5′ end of the gene were isolated, and analysis of these clones indicated that alternate first exons ∼1.5 kb apart and ∼ 45 kb upstream of a common second exon formed the basis of these transcripts. Genomic fragments that included the proximal 5′-flanking region of either transcript were able to direct expression of a reporter gene in lung epithelia and to bind Sp1 in nuclear extracts, confirming the presence of separate promoters that regulate β-ENaC expression.


2020 ◽  
Vol 23 (4) ◽  
pp. 25-33
Author(s):  
Boris V. Kovaliov ◽  
◽  
Vadim E. Pugach ◽  

The article is devoted to the analysis of the functions of the zero name of proper characters in V. V. Nabokov's short prose. The factors are highlighted on the basis of which an attempt can be made to classify zero names in Nabokov's texts. The first factor is the grammatical person from whom the story is told. Particular cases related to narration from the first and third person are analyzed. The second factor is who in the text is the bearer of the zero name: the main character or the minor one? The authors pay special attention to antonomazia as a way of replacing a proper name with a null name. In the course of the study, it turns out that antonomazia can be used as a means of characterizing a character, as well as a reference not to a specific image, but to a certain class. The third factor is the possible taboo of the name as a technique. In the course of the research, two types of tabooing of a proper name are distinguished: religious and sociopsychological. The second part of the article analyzes V. V. Nabokov's story «The Razor» as an example of a text, on the basis of which one can prove the assertion about the polyfunctionality of a null name. The nomination of each character is studied in detail. The authors conclude that the opposition of full and null names plays a key role in building conflict in the plot. Moreover, the opposition of different types of nominations is a technique that determines the structure of the text, its semantic and associative levels. Based on a review of a number of Nabokov's texts and a detailed analysis of «The Razor» story, eleven functions of the zero name of V. V. Nabokov's own characters are formulated.


1983 ◽  
Vol 49 (1) ◽  
pp. 177-193 ◽  
Author(s):  
Richard Bradley ◽  
Clive Hart

This paper investigates the distribution of artefacts and monuments in one part of the Peak District, in relation to the land use models put forward by Hawke-Smith in 1979. Although these distributions are generally consistent with his predictions, they suggest that communities in newly settled areas may have been of lower status than those near the henge monument of Arbor Low. This distinction is emphasized in the burial rite and by differential access to imported materials.


Author(s):  
James Haire

United and uniting churches have made a very significant contribution to the ecumenical movement. In seeking to assess that contribution, the chapter first defines what these churches are, considers the different types of union that have been created, examines the characteristics of these churches, and looks at the theological rationale for them. It goes on to trace the history of their formation from the beginning of the nineteenth century, and particularly during the years leading up to and following the Third Assembly of the World Council of Churches at New Delhi in 1961, under the influence of Lesslie Newbigin. Giving a theological assessment, it emphasizes that the existence of these churches, despite difficulties, provides places where the final unity of Christ’s one body is most clearly foreshadowed. They will always present proleptic visions of that goal.


2021 ◽  
Vol 1 (2) ◽  
pp. 155-164
Author(s):  
Darto Sachius

The study is titled characteristics Psalm of Praise. This study will seek and discover the characteristics and principles of psalm praise in the Book of Psalms. To discover the characteristics of the Praise Psalm used qualitative rather than experimental research methods. This qualitative method can also be used for reflective speculators. With four steps: analyzing the passage according to its elements, the second step is to recognize the language style of poetry and stories that are historical, the third reveals the original meaning of Hebrew poetry, the fourth looks for the characteristics of the Hebrew satra of the author's motives and goals. So that preserved by this method can be formulated characteristics from the Psalm of Praise. There are three important things to remember in the Psalm of praise is, first the Psalm of praise begins with an invitation to worship The Lord, The second psalm of praise continues by giving a description of the reasons why God is worthy of praise. Third is the psalm praise usually includes and sometimes ends with a further invitation to praise God therefore the proper title is the characteristics Psalm of Praise so that the awkwardness of distinguishing the interpretation of different types of psalm can be well cleared.


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