scholarly journals Genomic Characterization and Annotation of Two Novel Bacteriophages Isolated from a Wastewater Treatment Plant in Qatar

Author(s):  
Ramya Ramadoss ◽  
Fajer Al-Marzooqi ◽  
Basem Shomar ◽  
Valentin Alekseevich Ilyin ◽  
Annette Shoba Vincent

We report the genome sequences of Escherichia phage C600M2 (length, 88,162 bp; G+C content, 38.98%) and Escherichia phage CL1 (length, 87,820 bp; G+C content, 41.32%), which were isolated from a wastewater treatment plant in Qatar. Both Escherichia phage C600M2 and Escherichia phage CL1 genomes contain 128 protein-coding genes and 26 tRNAs.

2019 ◽  
Vol 8 (35) ◽  
Author(s):  
A. R. Stahlke ◽  
A. Z. Ozsoy ◽  
D. W. Bean ◽  
P. A. Hohenlohe

We announce the complete circularized mitochondrial genome assemblies of Diorhabda carinata and Diorhabda carinulata, beetle species introduced to North America for the biological control of invasive shrubs of the genus Tamarix L. (Tamaricaceae). The assemblies (16,232 and 16,298 bp, respectively) each comprise 13 protein-coding genes, 22 tRNAs, two rRNAs, and a noncoding region.


2020 ◽  
Vol 9 (15) ◽  
Author(s):  
Steven Batinovic ◽  
Daniel T. F. Rice ◽  
Robert J. Seviour ◽  
Steve Petrovski

We report the complete genome sequence of Moraxella osloensis strain YV1, which was isolated from a wastewater treatment plant in Australia. The YV1 genome comprises a 2,615,801-bp chromosome and four plasmids. Moraxella osloensis strain YV1 displays the distinctive morphology of Eikelboom morphotype 1863.


2020 ◽  
Vol 9 (8) ◽  
Author(s):  
Sushanta Deb ◽  
Jhasketan Badhai ◽  
Subrata K. Das

We report the draft genome sequences of Vibrio alginolyticus strain S6-61 and Vibrio diabolicus strain S7-71, isolated from the corals Pocillopora verrucosa and Fungia danai, respectively. The genomes of strains S6-61 and S7-71 contain 4,880 and 4,641 protein coding genes, respectively, and harbor genes associated with the ectoine biosynthesis pathway.


2018 ◽  
Vol 41 (5) ◽  
pp. 554-571 ◽  
Author(s):  
Cesar Casiano Flores ◽  
Hans Bressers ◽  
Carina Gutierrez ◽  
Cheryl de Boer

Purpose In Mexico, only 19.3 per cent of industrial water is treated (Green-Peace, 2014, pp. 3-4), whereas municipal treatment levels are approximately 50 per cent (CONAGUA, 2014a). This paper aims to focus on how the wastewater treatment plant policy, from a circular economy perspective, is affected by the governance context at the Presa Guadalupe sub-basin. Circular economy can contribute to water innovations that help in improving water quality. However, such benefits are not easily achieved. This case provides an example of the complexity and challenges that the implementation of a circular economy model can face. Design/methodology/approach Data are collected via semi-structured in-depth interviews with the stakeholders that are members of the Presa Guadalupe Commission. The contextual interaction theory (CIT) is the theoretical basis for this analysis (Boer de and Bressers, 2011; Bressers, 2009). Findings The findings show that the wastewater treatment plant policy plays an important role in a circular economy model. Some incentives towards a circular economy model are already in place; however, the hurdles of a top-down implementation perspective, low availability of resources, prioritisation of short-term results, lack of enforcement of the “polluter pays” principle and a linear model of water systems need to be overcome. If Mexico wants to move towards a circular economy model and if the government wants to enforce sustainable development principles, wastewater treatment is a challenge that must be addressed. Originality/value There are few studies in the circular economy literature that have analysed its implementation under a governance arrangement perspective.


2017 ◽  
Vol 5 (45) ◽  
Author(s):  
Rodney A. King ◽  
Tina M. Slowan-Pomeroy ◽  
Jodi E. Thomas ◽  
Tithe Ahmed ◽  
Katie L. Alexander ◽  
...  

ABSTRACT Bacteriophages AlleyCat, Edugator, and Guillsminger were isolated on Mycobacterium smegmatis mc2155 from enriched soil samples. All are members of mycobacteriophage subcluster K5, with genomes of 62,112 to 63,344 bp. Each genome contains 92 to 99 predicted protein-coding genes and one tRNA. Guillsminger is the first mycobacteriophage to carry an IS1380 family transposon.


2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Amr Metwally El-Kholy ◽  
Ahmed Yousry Akal

PurposeThis research investigates the financial viability risk factors that threaten the private investor's economic scheme in the public private partnership (PPP) wastewater treatment plant (WWTP) projects in Egypt. The aims of this study are to: (1) illustrate and cluster the financial viability risk factors in accordance with the PPP WWTP projects' nature, (2) assess the risk factors' criticality degrees according to their severity and frequency levels of the financial viability, and (3) pinpoint the suited allocation of the financial viability risk factors between the public and private parties.Design/methodology/approachRelying on the previous analysts' endeavors, the questionnaire method, the fuzzy synthetic evaluation approach and the hypothetical normal distribution curve model; the severity, frequency, criticality and allocation preference of 32 financial viability risk factors were assessed from 12 Egyptian PPP experts.FindingsThe data analysis yielded that foreign exchange risk, currency risk/inflation, license risk, construction cost-overrun and late site handover are the key factors in arising the financial viability risk issue in the PPP WWTP projects. Considering the discussion of these key risks, the study summarized that the financial viability's key risk factors are notably be affected by the economic, political and administrative circumstances of the host county. Additionally, the inflation lesion was found to be the core reason of most of the key risk factors.Originality/valueThis research originality stems from its contribution to address the gab in the PPP risk assessment literature of the concessionaire's financial viability in the WWTP projects in a country of developing economy as Egypt. This, first, enriches the scholarly based knowledge of the PPP projects' risk analysts of the developing countries. Accordingly, it moves the current PPP risk assessment research further to deeply apprehend these markets' risks. Second, it equips the policymakers in the public and private sectors of such projects with a map that clarifies their assigned risk factors and the responsibilities that each party should bear to generate a mutual stable investment environment for achieving their aims successfully. This, indeed, paves the way for more private investments to be involved in the developing markets' PPP projects with a profitable satisfactory level for the private concessionaire. In the same vein, more WWTP projects, which are highly needed for the public sector and its people, are executed.


2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3727-3732 ◽  
Author(s):  
Yingshun Cui ◽  
Sung-Geun Woo ◽  
Jangho Lee ◽  
Sahastranshu Sinha ◽  
Myung-Suk Kang ◽  
...  

A Gram-reaction-positive, rod-shaped, non-spore-forming bacterium (strain 2C1-5T) was isolated from activated sludge of an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were the type strains of Nocardioides nitrophenolicus (98.6 % similarity), N. kongjuensis (98.5 %), N. caeni (98.4 %), N. simplex (98.3 %), N. aromaticivorans (98.1 %) and N. ginsengisoli (97.5 %); the phylogenetic distance from other species with validly published names within the genus Nocardioides was greater than 3 %. Strain 2C1-5T was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and iso-C16 : 0, C16 : 0 and C17 : 1ω6c as the major fatty acids. The G+C content of the genomic DNA was 74.9 mol%. These chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain 2C1-5T to the genus Nocardioides . The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 2C1-5T from existing species with validly published names. Therefore, strain 2C1-5T represents a novel species of the genus Nocardioides , for which the name Nocardioides daeguensis sp. nov. is proposed, with the type strain 2C1-5T ( = JCM 17460T = KCTC 19799T).


2020 ◽  
Vol 9 (46) ◽  
Author(s):  
Heidi Lin ◽  
Michael Reeves ◽  
Melissa Acevedo ◽  
Kelsey Bass ◽  
Elizabeth Chau ◽  
...  

ABSTRACT Cluster EC ClearAsMud and cluster EA4 Kauala are lytic Siphoviridae bacteriophages that were isolated from soil in southern California using Microbacterium foliorum NRRL B-24224 as the host. The ClearAsMud and Kauala genomes are 52,987 bp and 39,378 bp, respectively, and contain 92 and 56 predicted protein-coding genes, respectively.


2017 ◽  
Vol 5 (16) ◽  
Author(s):  
Kirk R. Anders ◽  
Alex M. Murphy ◽  
William F. Ettinger ◽  
Douglas Kempthorne ◽  
Charles Kittridge ◽  
...  

ABSTRACT Mycobacteriophages DrHayes, Urkel, and SamuelLPlaqson were isolated from soil samples in Spokane, WA, using Mycobacterium smegmatis mc2155 grown at room temperature. The three genomes differ by only a few nucleotides, are 60,526 bp long, have 97 predicted protein-coding genes and one tRNA gene, and are members of subcluster K1.


2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Carlos Farkas ◽  
Raúl A. Donoso ◽  
Felipe Melis-Arcos ◽  
Carla Gárate-Castro ◽  
Danilo Pérez-Pantoja

Rhodococcus ruber R1 was isolated from a pulp mill wastewater treatment plant because of its ability to use methoxylated aromatics as growth substrates. We report the 5.56-Mb genome sequence of strain R1, which can provide insights into the biodegradation of lignin-derived phenolic monomers and potentially support processes for lignocellulose conversion.


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