scholarly journals MICy: a Novel Flow Cytometric Method for Rapid Determination of Minimal Inhibitory Concentration

2021 ◽  
Author(s):  
András Kállai ◽  
Márta Kelemen ◽  
Noémi Molnár ◽  
Adrienn Tropotei ◽  
Balázs Hauser ◽  
...  
Keyword(s):  
Clinical Decision Making ◽  
Susceptibility Testing ◽  
Inhibitory Concentration ◽  
Rapid Determination ◽  
Clinical Decision ◽  
Flow Cytometric Method ◽  
Microdilution Method ◽  
Flow Cytometric ◽  
Rapid Flow

MICy is a new, simple and rapid flow cytometry based antibiotic susceptibility testing (AST) method that produces susceptibility profile a workday earlier than the microdilution method or other classical phenotypic AST methods. Shortening the length of AST can accelerate clinical decision-making as targeted antibiotic treatment improves clinical outcomes and reduces mortality, duration of artificial ventilation, and length of stay in intensive care unit.

Rapid flow cytometric method for viability determination of solventogenic clostridia

2012 ◽  
Vol 57 (4) ◽  
pp. 307-311 ◽  
Author(s):  
Michaela Linhová ◽  
Barbora Branská ◽  
Petra Patáková ◽  
Jakub Lipovský ◽  
Petr Fribert ◽  
...  
Keyword(s):  
Flow Cytometric Method ◽  
Flow Cytometric ◽  
Rapid Flow ◽  
Viability Determination ◽  
Solventogenic Clostridia

scholarly journals Microscopic Analysis of Bacterial Inoculum Effect Using Micropatterned Biochip

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 300
Author(s):  
Jung Ho Hwang ◽  
Sang Young Lee ◽  
Jungil Choi
Keyword(s):  
Susceptibility Testing ◽  
Inhibitory Concentration ◽  
Microscopic Analysis ◽  
Microfluidic System ◽  
Timely Manner ◽  
Testing Conditions ◽  
Bacterial Inoculum ◽  
Clinical Environments ◽  
Inoculum Effect

Antimicrobial resistance has become a major problem in public health and clinical environments. Against this background, antibiotic susceptibility testing (AST) has become necessary to cure diseases in an appropriate and timely manner as it indicates the necessary concentration of antibiotics. Recently, microfluidic based rapid AST methods using microscopic analysis have been shown to reduce the time needed for the determination of the proper antibiotics. However, owing to the inoculum effect, the accurate measurement of the minimal inhibitory concentration (MIC) is difficult. We tested four standard bacteria: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis, against five different antibiotics: piperacillin, cefotaxime, amikacin, levofloxacin, and ampicillin. The results showed that overall, the microfluidic system has a similar inoculum effect compared to the conventional AST method. However, due to the different testing conditions and determination protocols of the growth of the microfluidic based rapid AST, a few results are not identical to the conventional methods using optical density. This result suggests that microfluidic based rapid AST methods require further research on the inoculum effect for practical use in hospitals and can then be used for effective antibiotic prescriptions.

A microfluidic microbial culture device for rapid determination of the minimum inhibitory concentration of antibiotics

The Analyst ◽  
2013 ◽  
Vol 138 (4) ◽  
pp. 1000 ◽  
Author(s):  
Rika Takagi ◽  
Junji Fukuda ◽  
Keiji Nagata ◽  
Yutaka Yawata ◽  
Nobuhiko Nomura ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Rapid Determination ◽  
Microbial Culture

scholarly journals Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology

2016 ◽  
Vol 54 (11) ◽  
pp. 2701-2706 ◽  
Author(s):  
Randall T. Hayden ◽  
Lani K. Clinton ◽  
Carolyn Hewitt ◽  
Terri Koyamatsu ◽  
Yilun Sun ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Clinical Decision Making ◽  
Susceptibility Testing ◽  
Laser Light ◽  
Accurate Determination ◽  
Antimicrobial Susceptibility Testing ◽  
Limiting Factor ◽  
Light Scatter ◽  
Content Type

The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) ofStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosawere tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility.

A flow cytometric method for determination of the interferon receptor IFNAR2 subunit in peripheral blood leukocyte subsets

2004 ◽  
Vol 50 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Shiro Tochizawa ◽  
Seiji Akamatsu ◽  
Yoshihiro Sugiyama ◽  
Masahiro Muraguchi ◽  
Yasukazu Ohmoto ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Leukocyte ◽  
Flow Cytometric Method ◽  
Flow Cytometric ◽  
Interferon Receptor ◽  
Blood Leukocyte
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