Features of Methyl Linoleate Oxidation in Triton X-100 Micellar Buffer Solutions

2020 ◽  
Vol 93 (7) ◽  
pp. 1090-1095
Author(s):  
D. V. Loshadkin ◽  
E. M. Pliss ◽  
O. T. Kasaikina
Keyword(s):  
Methyl Linoleate ◽  
Buffer Solutions ◽  
Triton X ◽  
Linoleate Oxidation

Stable nitroxyl radicals and hydroxylamines as inhibitors of methyl linoleate oxidation in micelles

2015 ◽  
Vol 64 (10) ◽  
pp. 2438-2443 ◽  
Author(s):  
I. V. Tikhonov ◽  
E. M. Pliss ◽  
L. I. Borodin ◽  
V. D. Sen’ ◽  
T. S. Kuznetsova
Keyword(s):  
Methyl Linoleate ◽  
Nitroxyl Radicals ◽  
Stable Nitroxyl Radicals ◽  
Linoleate Oxidation

Kinetic study and simulation of methyl linoleate oxidation in micelles

2015 ◽  
Vol 9 (1) ◽  
pp. 127-131 ◽  
Author(s):  
E. M. Pliss ◽  
D. V. Loshadkin ◽  
A. M. Grobov ◽  
T. S. Kuznetsova ◽  
A. I. Rusakov
Keyword(s):  
Methyl Linoleate ◽  
Kinetic Study ◽  
Linoleate Oxidation

scholarly journals Enhanced Visualization of the Fine Structure of the Stigmatic Surface of Citrus using Pre-fixation Washes

2012 ◽  
Vol 137 (5) ◽  
pp. 290-293
Author(s):  
Hazel Y. Wetzstein ◽  
S. Edward Law
Keyword(s):  
Stigmatic Surface ◽  
Surface Compounds ◽  
Buffer Solutions ◽  
Phylogenetic Studies ◽  
Structural Details ◽  
Crystalline Deposits ◽  
Stigmatic Exudate ◽  
Triton X ◽  
First Time ◽  
Scanning Electron

Stigma characteristics and morphology can be useful in taxonomic and phylogenetic studies, indicate relationships in stigma function and receptivity, and be valuable in evaluating pollen–stigma interactions. Problematic is that in some taxa, copious stigmatic exudate can obscure the fine structural details of the stigmatic surface. Such is the case for Citrus, which has a wet stigma type on which abundant exudate inundates surface papillae. The components of stigmatic surface compounds are highly heterogeneous and include carbohydrates, proteins, lipids, glycoproteins, and phenolic compounds. This study evaluated the efficacy of several pre-fixation wash treatments on removing surface exudate to visualize the underlying stigmatic surface. Wash treatments included various buffer solutions, surfactants, dilute acids/bases, and solvents. Stigmas prepared using conventional fixation methods in glutaraldehyde had considerable accumulations of reticulate surface deposits with stigmatic cells obscured. Pre-fixation washes containing solvents such as methanol, chloroform, and ethanol left accumulations of incompletely removed exudate and crystalline deposits. Alkaline water washes produced a crust-like deposit on stigma surfaces. Buffer washes left residues of plaque-like deposits with perforated areas. In contrast, excellent removal of stigmatic exudate was obtained with a pre-fixation wash composed of 0.2 M Tris buffer, pH 7.2, containing 0.2% Triton X-100 surfactant and allowed clear imaging of the stigma and surface papillae morphology. A central sinus and radially arranged openings on the stigmatic surface were clearly visible and shown for the first time using scanning electron microscopy (SEM).

SEM of non-coated hepatocellular cytoskeleton of perfused livers

1984 ◽  
Vol 42 ◽  
pp. 306-307
Author(s):  
S.W. French ◽  
N.C. Benson ◽  
C. Davis-Scibienski
Keyword(s):  
Ambient Temperature ◽  
Critical Point ◽  
Protease Inhibitors ◽  
Metal Deposition ◽  
Heat Damage ◽  
Detergent Extraction ◽  
Cytoskeletal Elements ◽  
Triton X ◽  
Excised Tissue

Previous SEM studies of liver cytoskeletal elements have encountered technical difficulties such as variable metal coating and heat damage which occurs during metal deposition. The majority of studies involving evaluation of the cell cytoskeleton have been limited to cells which could be isolated, maintained in culture as a monolayer and thus easily extracted. Detergent extraction of excised tissue by immersion has often been unsatisfactory beyond the depth of several cells. These disadvantages have been avoided in the present study. Whole C3H mouse livers were perfused in situ with 0.5% Triton X-100 in a modified Jahn's buffer including protease inhibitors. Perfusion was continued for 1 to 2 hours at ambient temperature. The liver was then perfused with a 2% buffered gluteraldehyde solution. Liver samples including spontaneous tumors were then maintained in buffered gluteraldehyde for 2 hours. Samples were processed for SEM and TEM using the modified thicarbohydrazide procedure of Malich and Wilson, cryofractured, and critical point dried (CPD). Some samples were mechanically fractured after CPD.

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