Fine structure of the giant cells induced by Meloidogyne javanica in lima bean

1984 ◽  
Vol 62 (3) ◽  
pp. 429-436 ◽  
Author(s):  
F. Fattah ◽  
J. M. Webster
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Light And Electron Microscopy ◽  
Lima Bean ◽  
Meloidogyne Javanica ◽  
Giant Cells ◽  
Egg Laying ◽  
Phaseolus Lunatus ◽  
Wide Range

Giant cells associated with egg-laying females of Meloidogyne javanica in lima bean, Phaseolus lunatus cv. L-136, were examined by light and electron microscopy. These giant cells have characteristics that are typical of nematode-induced giant cells in a wide range of hosts, but they differ in that they (i) are less closely associated with xylem vessels, (ii) contain a very large number of plastids which are devoid of starch grains, and (iii) contain several different forms of cytoplasmic crystals. One form of the crystal is associated with a large number of "spiny" vesicles. The possible role of these features, especially the crystals, in the giant cell response of lima bean is discussed.

The Role of Mitochondria in the Genesis of Neuroaxonal Dystrophy in the Brains of Aging Mice

1975 ◽  
Vol 33 ◽  
pp. 372-373
Author(s):  
J.E. Johnson
Keyword(s):  
Electron Microscopy ◽  
Present Report ◽  
Light And Electron Microscopy ◽  
Dorsal Column ◽  
Neuroaxonal Dystrophy ◽  
Nucleus Gracilis ◽  
Dystrophic Axons ◽  
The Brain ◽  
Nucleus Cuneatus

Although neuroaxonal dystrophy (NAD) has been examined by light and electron microscopy for years, the nature of the components in the dystrophic axons is not well understood. The present report examines nucleus gracilis and cuneatus (the dorsal column nuclei) in the brain stem of aging mice.Mice (C57BL/6J) were sacrificed by aldehyde perfusion at ages ranging from 3 months to 23 months. Several brain areas and parts of other organs were processed for electron microscopy.At 3 months of age, very little evidence of NAD can be discerned by light microscopy. At the EM level, a few axons are found to contain dystrophic material. By 23 months of age, the entire nucleus gracilis is filled with dystrophic axons. Much less NAD is seen in nucleus cuneatus by comparison. The most recurrent pattern of NAD is an enlarged profile, in the center of which is a mass of reticulated material (reticulated portion; or RP).

Fine structure and possible functions of the hermaphrodite duct of some pulmonate snails (Mollusca: Gastropoda)

1990 ◽  
Vol 48 (3) ◽  
pp. 68-69
Author(s):  
Alan N. Hodgson
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Seminal Vesicle ◽  
Reproductive Biology ◽  
Light Microscope ◽  
Light Microscope Level ◽  
Transmission Electron ◽  
Standard Techniques

The hermaphrodite duct of pulmonate snails connects the ovotestis to the fertilization pouch. The duct is typically divided into three zones; aproximal duct which leaves the ovotestis, the middle duct (seminal vesicle) and the distal ovotestis duct. The seminal vesicle forms the major portion of the duct and is thought to store sperm prior to copulation. In addition the duct may also play a role in sperm maturation and degredation. Although the structure of the seminal vesicle has been described for a number of snails at the light microscope level there appear to be only two descriptions of the ultrastructure of this tissue. Clearly if the role of the hermaphrodite duct in the reproductive biology of pulmonatesis to be understood, knowledge of its fine structure is required.Hermaphrodite ducts, both containing and lacking sperm, of species of the terrestrial pulmonate genera Sphincterochila, Levantina, and Helix and the marine pulmonate genus Siphonaria were prepared for transmission electron microscopy by standard techniques.

scholarly journals Morphological Alterations and Increased S100B Expression in Epidermal Langerhans Cells Detected in Skin from Patients with Progressive Vitiligo

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 579
Author(s):  
Fei Yang ◽  
Lingli Yang ◽  
Lanting Teng ◽  
Huimin Zhang ◽  
Ichiro Katayama
Keyword(s):  
Electron Microscopy ◽  
Langerhans Cells ◽  
Critical Role ◽  
Light And Electron Microscopy ◽  
Morphological Alterations ◽  
Birbeck Granules ◽  
Immuno Electron Microscopy ◽  
Skin Biopsies ◽  
Epidermal Langerhans Cells

The role of Langerhans cells (LCs) in vitiligo pathogenesis remains unclear, with published studies reporting contradictory results regarding the quantity of LCs and no data on the features of LCs in vitiligo. Here, we aimed to analyze the presence, density, and morphological features of LCs in the epidermis of patients with vitiligo. Skin biopsies were stained for LCs using anti-CD1a/anti-langerin antibodies and analyzed by immunocytochemistry with light and electron microscopy. Compared with healthy controls, we detected significantly increased numbers of epidermal LCs in lesional skin from vitiligo in the progressive state. These LCs exhibited striking morphological alterations, including an elevated number of dendrites, with increased length and more branches than dendrites from controls. Ultrastructure examination via immuno-electron microscopy revealed markedly reduced Birbeck granules (BGs) and shorter BG rods in LCs from progressive vitiligo, with higher expression of langerin. Additionally, expression of S100B, the activity biomarker of vitiligo, was increased in these LCs. This work provides new insight on the cellular composition of LCs in vitiliginous skin, revealing altered morphology and increased LC numbers, with elevated S100B expression. Our data suggest LCs might play a critical role in vitiligo pathogenesis and thus may represent a novel therapeutic target for this disease.

Intestinal Lesions Containing Coronavirus-like Particles in Neonatal Necrotizing Enterocolitis: An Ultrastructural Analysis

PEDIATRICS ◽  
1984 ◽  
Vol 73 (2) ◽  
pp. 218-224
Author(s):  
S. Rousset ◽  
O. Moscovici ◽  
P. Lebon ◽  
J. P. Barbet ◽  
P. Helardot ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Small Intestine ◽  
Necrotizing Enterocolitis ◽  
Intestinal Mucosa ◽  
Light And Electron Microscopy ◽  
Anaerobic Bacteria ◽  
Intestinal Lesions ◽  
Maternity Hospitals ◽  
Neonatal Necrotizing Enterocolitis

Since the outbreaks of neonatal necrotizing enterocolitis occurring in maternity hospitals of Paris and suburbs in 1979-1980, it has been possible to examine by light and electron microscopy gut specimens from ten newborns with this illness. Coronavirus-like particles, enclosed in intracytoplasmic vesicles of damaged epithelial cells of the intestinal mucosa, were observed in the small intestine, appendix, and colon. The ultrastructural study, supported by bacteriologic findings, suggests the role of coronavirus-like particles in the appearance of the lesions. Secondary proliferation of mainly anaerobic bacteria, probably responsible for pneumatosis, may aggravate the disease.

The Biology of Bothrimonus (=Diplocotyle) (Pseudophyllidea: Cestoda): Detailed Morphology and Fine Structure

1974 ◽  
Vol 31 (2) ◽  
pp. 147-153 ◽  
Author(s):  
M. D. B. Burt ◽  
I. M. Sandeman
Keyword(s):  
Electron Microscopy ◽  
Nervous System ◽  
Fine Structure ◽  
Functional Morphology ◽  
Light And Electron Microscopy ◽  
Nerve Fibers ◽  
Fish Host ◽  
Extensive System ◽  
And Function

Light and electron microscopy were used to describe the functional morphology of Bothrimonus sturionis in detail. In particular, the musculature, nervous system, osmoregulatory system, and tegument are dealt with, and the findings compared with those of other workers. The musculature of the scolex consists of several interrelated systems, the structure of each being discussed in relation to its function. Associated with the regular nervous system, considered typical of cestodes, is an extensive system of giant nerve fibers. The osmoregulatory system is unusual in that there are lateral "excretory" pores in many proglottides which open directly to the exterior of the worm. The microtriches of the tegument are long, like those of other primitive cestodes, and are covered by a noncellular sheath while the worm is in its gammarid host. The sheath is lost when the worm becomes established in its fish host; the nature and function of the sheath are discussed.

Fine Structure of the Eye of a Nudibranch Mollusc, Hermissenda Crassicornis

1967 ◽  
Vol 2 (3) ◽  
pp. 349-358
Author(s):  
R. M. EAKIN ◽  
JANE A. WESTFALL ◽  
M. J. DENNIS
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Optic Nerve ◽  
Light And Electron Microscopy ◽  
The Other ◽  
Sensory Cells ◽  
Nerve Fibres ◽  
Supporting Cells ◽  
Small Bodies ◽  
Receptor Cells

The eye of a nudibranch, Hermissenda crassicornis, was studied by light and electron microscopy. Three kinds of cells were observed: large sensory cells, each bearing at one end an array of microvilli (rhabdomere) and at the other end an axon which leaves the eye by the optic nerve; large pigmented supporting cells; and small epithelial cells, mostly corneal. There are five sensory cells, and the same number of nerve fibres in the optic nerve. The receptor cells contain an abundance of small vesicles, 600-800 Å in diameter. The lens is a spheroidal mass of osmiophilic, finely granular material. A basal lamina and a capsule of connective tissue enclose the eye. In some animals the eye is ‘infected’ with very small bodies, 4-5 µ in diameter, thought to be symbionts.

Observations on the nephridial system of the cestode Moniezia expansa (Rud., 1805)

Parasitology ◽  
1969 ◽  
Vol 59 (2) ◽  
pp. 449-459 ◽  
Author(s):  
R. E. Howells
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Epithelial Cells ◽  
Connective Tissue ◽  
Intercellular Space ◽  
Technical Assistance ◽  
Light And Electron Microscopy ◽  
Flame Cell ◽  
Research Scholarship ◽  
Research Facilities

The nephridial system of M. expansa has been studied using light and electron microscopy, and a number of histochemical techniques have been used on sections of the worm. The organization of the nephridial system and the fine structure of the flame cells and the nephridial ducts are described. Pores, which connect the nephridial lumen to the intercellular space of the connective tissue, exist at the junction of a flame cell and a nephridial duct. These pores may be considered nephrostomes and the system therefore is not protonephridial as defined by Hyman (1951).The epithelium lining the nephridial ducts has a structure which suggests that it is metabolically active. It is postulated that the beating of the cilia of the flame cells draws fluid into the ducts via the nephrostomes, with absorption and/or secretion of solutes being carried out by the epithelial cells of the duct walls. The function of the nephridial system is discussed.I am grateful to Professor James Brough for the provision of research facilities at the Department of Zoology, University College, Cardiff, andtoDrD. A. Erasmus for much helpful advice during the course of the work. I wish to thank Professors W. Peters and T. Wilson for critically reading the manuscript and Miss M. Williams and Mr T. Davies for expert technical assistance.I also wish to thank the Veterinary Inspector and his staff at the Roath Abattoir, Cardiff, for their kind co-operation and assistance in obtaining material.The work was carried out under the tenure of an S.R.C. research scholarship.

scholarly journals The Fine Structure of the Wheat Scutellum During Germination

1972 ◽  
Vol 25 (3) ◽  
pp. 469 ◽  
Author(s):  
JG Swift ◽  
TP O'brien
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Epithelial Cells ◽  
Light And Electron Microscopy ◽  
Cell Types ◽  
Wall Material ◽  
Cytological Evidence ◽  
Starch Grains ◽  
Parenchyma Cells ◽  
Cytological Changes

The cytological changes that take place in the scutellar epithelium and parenchyma during the first 5 days of germination are described by light and electron microscopy. Within 6 hr small starch grains appear in the plastids of both cell types and the size and number of starch grains increase gradually as germination proceeds. Later in germination starch disappears again from the plastids in the epithelial cells, but large starch grains still remain in the parenchyma cells. The reserves of the protein bodies are hydrolysed and the residual vacuoles undergo extensive coales-cence. Modifications in the appearance of the wall material of the epithelial cells as these cells elongate are illustrated and possible functional bases for these changes are suggested. The cells of the scutellar epithelium show no cytological evidence for their known functions of diastase secretion and nutrient absorption.

In situ studies on the cytochemistry and ultrastructure of a symbiotic marine dinoflagellate

1968 ◽  
Vol 48 (2) ◽  
pp. 349-366 ◽  
Author(s):  
D. L. Taylor
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Light And Electron Microscopy ◽  
Structural Morphology ◽  
Invertebrate Species ◽  
Algal Symbionts ◽  
Anemonia Sulcata ◽  
Mode Of Life ◽  
Structural Adaptations

The intertidal actinian Anemonia sulcata is known to harbour yellow-brown algal symbionts which are similar in appearance to the zooxanthellae of hermatypic, or reef-building, corals and a number of other invertebrate species. The cytochemistry and structural morphology of the zooxanthella has been studied by light and electron microscopy, to help define it taxonomically and to reveal something about its relations with the actinian. These investigations confirm that it is a dinoflagellate and have revealed several structural adaptations which are formed as a result of the peculiar mode of life adopted by this alga. Of significance is the fine structure of the periplast, which may have a considerable bearing upon the type of relationship which can exist between the host and its symbiont. These findings are discussed in terms of other known instances of algal-invertebrate symbiosis.

scholarly journals Female genital structures in several families of Centropagoidea (Copepoda: Calanoida)

1998 ◽  
Vol 353 (1369) ◽  
pp. 721-736 ◽  
Author(s):  
Roxane-M. Barthélémy ◽  
Corinne Cuoc ◽  
Danielle Defaye ◽  
Michel Brunet ◽  
Jacques Mazza
Keyword(s):  
Electron Microscopy ◽  
Seminal Receptacle ◽  
Genital Atrium ◽  
Mating Behaviour ◽  
Light And Electron Microscopy ◽  
Egg Laying ◽  
Common Pattern ◽  
Transmission Electron ◽  
Mode Of Operation ◽  
Female Genital

The female genital structures of 21 calanoid species belonging to the families Candaciidae, Centropagidae, Pontellidae, Sulcanidae, Temoridae and Tortanidae were studied using light and electron microscopy (scanning electron microscopy, transmission electron microscopy). Except the monotypic Sulcanidae, their organization conforms to a common pattern characterized by egg–laying ducts opening through paired adjacent gonopores into a small cavity, the genital atrium, covered by a genital operculum and opening to the outside through a distal atrial slit. No seminal receptacle is present and the seminal products must be stored for a brief period in the genital atrium.The genital structures occupy a well delimited genital area, the main changes in which concern their position on the ventral face of the genital double–somite, and operculum morphology. Their organization and possible mode of operation are compared with those of other calanoid families. A possible correlation between absence of seminal receptacles and mating behaviour is discussed.

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