New techniques and applications for epi-illumination light microscopy

1995 ◽  
Vol 73 (11) ◽  
pp. 1842-1847 ◽  
Author(s):  
Christian R. Lacroix ◽  
Judith MacIntyre
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Depth Of Field ◽  
New Techniques ◽  
Illumination System ◽  
Simple Preparation ◽  
Stereo Microscope ◽  
Material Saving ◽  
Scanning Electron

This modification to the technique of epi-illumination light microscopy makes use of a new system of lenses that replaces expensive and not readily available dipping cone objectives. The newer objectives offer at least comparable resolution and depth of field, along with simple preparation procedures. An epi-illumination system is a good intermediate between the stereo microscope and a scanning electron microscope, offering magnification at high power that can aid in evaluation of potential scanning electron microscope specimens, as well as the time- and material-saving feature of being able to eliminate unsuitable scanning electron microscope specimens. Key words: technique, epi-illumination, morphogenesis, vegetative apex, primordium, staining.

The Broad Applications of the Scanning Electron Microscope

1969 ◽  
Vol 27 ◽  
pp. 20-21
Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Surface Topography ◽  
Great Depth ◽  
Resolving Power ◽  
Depth Of Field ◽  
Pictorial Representations ◽  
Scanning Electron

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.

Visualization of Internal Skin Structures with the Scanning Electron Microscope

1969 ◽  
Vol 27 ◽  
pp. 46-47
Author(s):  
Emil Bernstein
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Great Depth ◽  
Hair Follicles ◽  
Depth Of Field ◽  
Pig Skin ◽  
Realistic Picture ◽  
Main Components ◽  
Scanning Electron

An interesting method for examining structures in g. pig skin has been developed. By modifying an existing technique for splitting skin into its two main components—epidermis and dermis—we can in effect create new surfaces which can be examined with the scanning electron microscope (SEM). Although this method is not offered as a complete substitute for sectioning, it provides the investigator with a means for examining certain structures such as hair follicles and glands intact. The great depth of field of the SEM complements the technique so that a very “realistic” picture of the organ is obtained.

scholarly journals Protocirrineris (Polychaeta:Cirratulidae) in South Africa and description of two new species

2019 ◽  
Vol 67 (S5) ◽  
pp. S70-S80
Author(s):  
Rodolfo Elías ◽  
María Andrea Saracho-Bottero ◽  
Carol Anne Simon
Keyword(s):  
Electron Microscope ◽  
New Species ◽  
Scanning Electron Microscope ◽  
Southern Africa ◽  
New Techniques ◽  
Tropical Regions ◽  
Diagnostic Characters ◽  
Two New Species ◽  
The Status ◽  
Scanning Electron

Introduction: The knowledge of polychaetes in the subtropical region of Africa benefited from the activity of J. Day. However, 50 years after the publication of his Monograph of the Polychaeta of southern Africa, it is necessary to reconsider the identity of the Cirratulidae due to changes in the diagnostic characters and new approaches to the taxonomy of the group to corroborate the status of cosmopolitan species in this region. Objective: We hypothesize that biodiversity of multitentacular Cirratulidae polychaetes has been significantly underestimated in southern Africa. Methods: The present work analyzes material deposited in the Iziko museum, as well as recently collected specimens, using scanning electron microscope to identify them. Results: The material corresponds to two new species belonging to the genus Protocirrineris. Protocirrineris strandloperarum sp. nov. is characterized by having the tentacular filaments between the chaetigers 5 to 10-12 and the first pair of branchiae from chaetiger 7, and P. magalhaesi sp. nov. is characterized by having tentacular filaments between chaetigers 4-8 and the first pair of branchiae from chaetigers 2 or 3. Descriptions of these species, with light and scanning electron microscope images, are given. Schematic drawings of the two new species are shown comparatively with diagnostic characters. Conclusions: The use of new techniques enables discovery of new taxonomic characters and two new species of the genus. The diversity of Cirratulidae polychaetes is underestimated also in the subtropical and tropical regions of Africa.

Additions to the technique of epi-illumination light microscopy for the study of floral and vegetative apices

1989 ◽  
Vol 67 (6) ◽  
pp. 1739-1743 ◽  
Author(s):  
W. A. Charlton ◽  
A. D. Macdonald ◽  
U. Posluszny ◽  
C. P. Wilkins
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Staining Techniques ◽  
Scanning Electron

Various staining techniques for apices destined for epi-illumination light microscopy are described and discussed. Appropriate modifications to photographic procedures are described. A technique is described for the subsequent observation of specimens in a scanning electron microscope fitted with a cryo-stage.

scholarly journals Toward Operations in a Surgical Scenario: Characterization of a Microgripper via Light Microscopy Approach

2019 ◽  
Vol 9 (9) ◽  
pp. 1901 ◽  
Author(s):  
Federica Vurchio ◽  
Pietro Ursi ◽  
Francesco Orsini ◽  
Andrea Scorza ◽  
Rocco Crescenzi ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Statistical Analysis ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Mechanical Systems ◽  
Optical Microscope ◽  
Micro Electro Mechanical Systems ◽  
Mems Technology ◽  
Scanning Electron

Micro Electro Mechanical Systems (MEMS)-Technology based micro mechanisms usually operate within a protected or encapsulated space and, before that, they are fabricated and analyzed within one Scanning Electron Microscope (SEM) vacuum specimen chamber. However, a surgical scenario is much more aggressive and requires several higher abilities in the microsystem, such as the capability of operating within a liquid or wet environment, accuracy, reliability and sophisticated packaging. Unfortunately, testing and characterizing MEMS experimentally without fundamental support of a SEM is rather challenging. This paper shows that in spite of large difficulties due to well-known physical limits, the optical microscope is still able to play an important role in MEMS characterization at room conditions. This outcome is supported by the statistical analysis of two series of measurements, obtained by a light trinocular microscope and a profilometer, respectively.

Comparison of Larval Stored Product Beetle Mandibles by Light Microscope and Scanning Electron Microscope

1981 ◽  
Vol 64 (1) ◽  
pp. 199-224
Author(s):  
John E Kvenberg
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Morphological Characteristics ◽  
Scanning Electron ◽  
Conventional Light Microscopy

Abstract Larval stored product beetle mandibles were studied by comparing images made by scanning electron microscopy with those made by conventional light microscopy. Discussion of morphological characteristics is based on illustrations of 25 species

scholarly journals Taxonomy and distribution of Pectinariidae (Annelida) from Iceland with a comparative analysis of uncinal morphology

2020 ◽  
Author(s):  
Julio Parapar ◽  
Verónica Palomanes ◽  
Gudmundur V. Helgason ◽  
Juan Moreira
Keyword(s):  
Electron Microscope ◽  
Comparative Analysis ◽  
Scanning Electron Microscope ◽  
Southern Coast ◽  
Bathymetric Distribution ◽  
The Family ◽  
Stereo Microscope ◽  
Northeast Coast ◽  
Scanning Electron

Based on samples collected during the BIOICE project off Iceland, four species of marine annelids belonging to the family Pectinariidae were identified: Amphictene auricoma (O.F. Müller, 1776), Cistenides granulata (Linnaeus, 1767), Cistenides hyperborea Malmgren, 1865 and Lagis koreni Malmgren, 1866. Taxonomic remarks and data on geographical and bathymetric distribution are presented. The distribution of each species off Iceland was evaluated and two patterns were defined: C. granulata and C. hyperborea were mainly found in waters off the northeast coast, while A. auricoma and L. koreni were found on the southern coast. Several body characters with taxonomic value in this family were reviewed under the stereo microscope and scanning electron microscope, with special emphasis on the neuropodial uncini. Remarks on these special chaetae are included in the diagnoses.

Morphology of the perineal pattern of the root-knot nematodes Meloidogyne hapla and M. incognita

1975 ◽  
Vol 53 (4) ◽  
pp. 370-373 ◽  
Author(s):  
R. H. Mulvey ◽  
P. W. Johnson ◽  
J. L. Townshend ◽  
J. W. Potter
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Meloidogyne Hapla ◽  
Root Knot Nematodes ◽  
Scanning Electron

Scanning electron microscope studies of Meloidogyne hapla and M. incognita revealed features of the female perineal pattern previously obscure under light microscopy. M. hapla is characterized by a deep invagination of the cuticle between the anal and vulval areas and a strong demarcation of dorsal and ventral striae. M. incognita lacks the infolding of the cuticle and is characterized by forked striae.

scholarly journals Guidance for Choosing When to Use Electron and/or Light Microscopy and Related ASTM E4 Standards

2004 ◽  
Vol 12 (2) ◽  
pp. 26-27 ◽  
Author(s):  
R. C. Nester
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Imaging Systems ◽  
Light Micrograph ◽  
Investigative Techniques ◽  
Scanning Electron

Technical publications sometimes include scanning electron images to characterize a microstructure, when the relevant structure could have very easily been illustrated using a simple light micrograph. When should one use a light generated image? What are the advantages/disadvantages of an electron generated image, particularly; one generated using a scanning electron microscope (SEM)? While there is some overlap in the capabilities of these imaging systems; in general, they are complementary tools, each with their own uses. Standards under the jurisdiction of ASTM Committee E4 on Metallography offer guidance to both new and experienced users of both investigative techniques.

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