Intestinal parasites of gray wolves (Canis lupus) in northern and western Canada

2016 ◽  
Vol 94 (9) ◽  
pp. 643-650 ◽  
Author(s):  
Janna M. Schurer ◽  
Michael Pawlik ◽  
Anna Huber ◽  
Brett Elkin ◽  
H. Dean Cluff ◽  
...  
Keyword(s):  
Canis Lupus ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Parasite Fauna ◽  
Detection Methods ◽  
Taenia Hydatigena ◽  
Gray Wolves ◽  
Wide Range ◽  
Parasite Spillover ◽  
Diphyllobothrium Latum

Gray wolves (Canis lupus L., 1758) are mobile opportunistic predators that can be infected by a wide range of parasites, with many acquired via predator–prey relationships. Historically, many of these parasites were identified only to genus or family, but genetic tools now enable identification of parasite fauna to species and beyond. We examined 191 intestines from wolves harvested for other purposes from regions in the Northwest Territories, British Columbia, Saskatchewan, and Manitoba. Adult helminths were collected from intestinal contents for morphological and molecular identification, and for a subset of wolves, fecal samples were also analyzed to detect helminth eggs and protozoan (oo)cysts. Using both detection methods, we found that 83% of 191 intestines contained one or more parasite species, including cestodes (Taenia spp., Echinococcus spp., and Diphyllobothrium sp.), nematodes (Uncinaria stenocephala Railliet, 1884, Trichuris spp., Physaloptera spp., and Toxascaris leonina (von Linstow, 1902)), a trematode (Alaria sp.), and protozoa (Sarcocystis spp., Giardia sp., and Cryptosporidium spp.). Molecular characterization identified one species of Diphyllobothrium (Diphyllobothrium latum (L., 1758) Cobbold, 1858), three species of Taenia (Taenia krabbei Moniez, 1879, Taenia hydatigena Pallas, 1766, and Taenia multiceps Leske, 1786), and two Giardia duodenalis (Davaine) Deschiens, 1921 assemblages (B and C). These results demonstrate the diverse diet of wolves and illustrate the possibility of parasite spillover among wildlife, domestic animals, and people.

scholarly journals Cryptosporidium and Giardia as water contaminant pathogens in Hungary

2013 ◽  
Vol 154 (46) ◽  
pp. 1836-1842 ◽  
Author(s):  
Judit Plutzer
Keyword(s):  
Epidemiological Study ◽  
Simple Procedure ◽  
Sewage Treatment ◽  
Giardia Duodenalis ◽  
Cost Effective ◽  
Control Measures ◽  
Detection Methods ◽  
Forest Environment ◽  
Detection Technology ◽  
Wide Range

Introduction: Many species of Cryptosporidium, and two assemlages of Giardia duodenalis cause typically acute diaorrhoea in human. The oocysts and cysts of these parasites excreted in faeces are capable of infecting other hosts and those are environmentally stable. Aim: The aims of the study were to evaluate the prevalence and genotypes of Cryptosporidium and Giardia species from different water sources as well as to monitor and characterize the (oo)cyst contamination sources in watersheds. In addition, an epidemiological study was performed in three selected settlements. Method: Wide range of modern epidemiological and molecular detection methods have been applied. Results: (Oo)cysts densities were associated with water receiving effluents of sewage treatment plants or originating from a forest environment. It was confirmed, that cattle can be a source of Cryptosporidium oocysts at watersheds and aquatic birds can play a role in the environmental dissemination of these protozoa. The epidemiological study demonstrated a specific epidemiological situation, giving essential evidence about giardiasis in asymptomatic carriers. The applied novel detection technology was found to be cost effective and simple procedure for screening catchments to identify those that require further treatment and more detailed microscopic counts. Conclusions: The presented results contribute to a better understanding the epidemiology and relevance of waterborne parasites, their surveillance and performance of future control measures to prevent waterborne infections in Hungary. Orv. Hetil., 154(46), 1836–1842.

scholarly journals Detectivity optimization to detect of ultraweak light fluxes with an EM-CCD as binary photon counter array

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ibtissame Khaoua ◽  
Guillaume Graciani ◽  
Andrey Kim ◽  
François Amblard
Keyword(s):  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
Photon Counting ◽  
Detection Methods ◽  
Strong Nonlinearity ◽  
Wide Range ◽  
Depth Analysis ◽  
Spatially Extended ◽  
Extended Sources ◽  
Photon Counting Mode

AbstractFor a wide range of purposes, one faces the challenge to detect light from extremely faint and spatially extended sources. In such cases, detector noises dominate over the photon noise of the source, and quantum detectors in photon counting mode are generally the best option. Here, we combine a statistical model with an in-depth analysis of detector noises and calibration experiments, and we show that visible light can be detected with an electron-multiplying charge-coupled devices (EM-CCD) with a signal-to-noise ratio (SNR) of 3 for fluxes less than $$30\,{\text{photon}}\,{\text{s}}^{ - 1} \,{\text{cm}}^{ - 2}$$ 30 photon s - 1 cm - 2 . For green photons, this corresponds to 12 aW $${\text{cm}}^{ - 2}$$ cm - 2 ≈ $$9{ } \times 10^{ - 11}$$ 9 × 10 - 11 lux, i.e. 15 orders of magnitude less than typical daylight. The strong nonlinearity of the SNR with the sampling time leads to a dynamic range of detection of 4 orders of magnitude. To detect possibly varying light fluxes, we operate in conditions of maximal detectivity $${\mathcal{D}}$$ D rather than maximal SNR. Given the quantum efficiency $$QE\left( \lambda \right)$$ Q E λ of the detector, we find $${ \mathcal{D}} = 0.015\,{\text{photon}}^{ - 1} \,{\text{s}}^{1/2} \,{\text{cm}}$$ D = 0.015 photon - 1 s 1 / 2 cm , and a non-negligible sensitivity to blackbody radiation for T > 50 °C. This work should help design highly sensitive luminescence detection methods and develop experiments to explore dynamic phenomena involving ultra-weak luminescence in biology, chemistry, and material sciences.

scholarly journals Defects and uncertainties of adhesively bonded composite joints

2021 ◽  
Vol 3 (9) ◽  
Author(s):  
Sadik Omairey ◽  
Nithin Jayasree ◽  
Mihalis Kazilas
Keyword(s):  
Composite Materials ◽  
Composite Structures ◽  
Production Efficiency ◽  
Adhesive Bonding ◽  
Detection Methods ◽  
Bonded Joints ◽  
Adhesively Bonded ◽  
Adhesively Bonded Joints ◽  
Wide Range ◽  
Bonded Composite

AbstractThe increasing use of fibre reinforced polymer composite materials in a wide range of applications increases the use of similar and dissimilar joints. Traditional joining methods such as welding, mechanical fastening and riveting are challenging in composites due to their material properties, heterogeneous nature, and layup configuration. Adhesive bonding allows flexibility in materials selection and offers improved production efficiency from product design and manufacture to final assembly, enabling cost reduction. However, the performance of adhesively bonded composite structures cannot be fully verified by inspection and testing due to the unforeseen nature of defects and manufacturing uncertainties presented in this joining method. These uncertainties can manifest as kissing bonds, porosity and voids in the adhesive. As a result, the use of adhesively bonded joints is often constrained by conservative certification requirements, limiting the potential of composite materials in weight reduction, cost-saving, and performance. There is a need to identify these uncertainties and understand their effect when designing these adhesively bonded joints. This article aims to report and categorise these uncertainties, offering the reader a reliable and inclusive source to conduct further research, such as the development of probabilistic reliability-based design optimisation, sensitivity analysis, defect detection methods and process development.

scholarly journals Current Trends and Challenges for Rapid SMART Diagnostics at Point-of-Site Testing for Marine Toxins

Sensors ◽  
2021 ◽  
Vol 21 (7) ◽  
pp. 2499
Author(s):  
Michael Dillon ◽  
Maja A. Zaczek-Moczydlowska ◽  
Christine Edwards ◽  
Andrew D. Turner ◽  
Peter I. Miller ◽  
...  
Keyword(s):  
Method Development ◽  
Regulatory Control ◽  
Detection Methods ◽  
Control Individual ◽  
Mouse Bioassay ◽  
Future Market ◽  
Site Testing ◽  
Wide Range ◽  
Development And Validation ◽  
Analytical Standards

In the past twenty years marine biotoxin analysis in routine regulatory monitoring has advanced significantly in Europe (EU) and other regions from the use of the mouse bioassay (MBA) towards the high-end analytical techniques such as high-performance liquid chromatography (HPLC) with tandem mass spectrometry (MS). Previously, acceptance of these advanced methods, in progressing away from the MBA, was hindered by a lack of commercial certified analytical standards for method development and validation. This has now been addressed whereby the availability of a wide range of analytical standards from several companies in the EU, North America and Asia has enhanced the development and validation of methods to the required regulatory standards. However, the cost of the high-end analytical equipment, lengthy procedures and the need for qualified personnel to perform analysis can still be a challenge for routine monitoring laboratories. In developing regions, aquaculture production is increasing and alternative inexpensive Sensitive, Measurable, Accurate and Real-Time (SMART) rapid point-of-site testing (POST) methods suitable for novice end users that can be validated and internationally accepted remain an objective for both regulators and the industry. The range of commercial testing kits on the market for marine toxin analysis remains limited and even more so those meeting the requirements for use in regulatory control. Individual assays include enzyme-linked immunosorbent assays (ELISA) and lateral flow membrane-based immunoassays (LFIA) for EU-regulated toxins, such as okadaic acid (OA) and dinophysistoxins (DTXs), saxitoxin (STX) and its analogues and domoic acid (DA) in the form of three separate tests offering varying costs and benefits for the industry. It can be observed from the literature that not only are developments and improvements ongoing for these assays, but there are also novel assays being developed using upcoming state-of-the-art biosensor technology. This review focuses on both currently available methods and recent advances in innovative methods for marine biotoxin testing and the end-user practicalities that need to be observed. Furthermore, it highlights trends that are influencing assay developments such as multiplexing capabilities and rapid POST, indicating potential detection methods that will shape the future market.

A first genetic assessment of the newly introduced Isle Royale gray wolves (Canis lupus)

2021 ◽  
Author(s):  
Samuel D. Hervey ◽  
Linda Y. Rutledge ◽  
Brent R. Patterson ◽  
Mark C. Romanski ◽  
John A. Vucetich ◽  
...  
Keyword(s):  
Canis Lupus ◽  
Isle Royale ◽  
Gray Wolves ◽  
Genetic Assessment

scholarly journals Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

2020 ◽  
Vol 9 (1) ◽  
pp. 54
Author(s):  
Salem Belkessa ◽  
Daniel Thomas-Lopez ◽  
Karim Houali ◽  
Farida Ghalmi ◽  
Christen Rune Stensvold
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Specific Pcr ◽  
Stool Samples ◽  
Pcr Targeting ◽  
Assemblage B

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

Effect of semen collection method on sperm motility of gray wolves (Canis lupus) and domestic dogs (C. l. familiaris)

2011 ◽  
Vol 76 (5) ◽  
pp. 975-980 ◽  
Author(s):  
Bruce W. Christensen ◽  
Cheryl S. Asa ◽  
Chong Wang ◽  
Lindsey Vansandt ◽  
Karen Bauman ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Canis Lupus ◽  
Domestic Dogs ◽  
Collection Method ◽  
Gray Wolves ◽  
Semen Collection

Protozoan infection in archaeological material

1970 ◽  
Vol 80 (1) ◽  
Author(s):  
Marcelo Luiz Carvalho Gonçalves ◽  
Cassius Schnell ◽  
Luciana Sianto ◽  
Francoise Bouchet ◽  
Mathieu Le Bailly ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
19Th Century ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Human Feces ◽  
Archaeological Material ◽  
The Past ◽  
Protozoan Infection ◽  
Protein Molecules ◽  
The 19Th Century

The identification of parasites in ancient human feces is compromised by differential preservation of identifiable parasite structures. However, protein molecules can survive the damage of the environment. It was possible to detected antigen of Entamoeba histolytica and Giardia duodenalis in historic and prehistoric human fecal remains using two enzyme immunoassay (ELISA) kits with monoclonal antibodies specific for E. histolytica and G. duodenalis, respectively. Specimens of desiccated feces and ancient latrine sediment from the New and the Old World were examined. The ELISA detected E. histolytica antigen in samples from Argentina, USA, France, Belgium, and Switzerland, dated to about 5300 years BP to the 19th Century AD. G. duodenalis antigen was detected in samples from USA, Belgium, and Germany, dated to about 1200 AD, 1600 AD, and 1700 AD. The detection of protozoan antigen using immunoassays is a reliable tool for the study of intestinal parasites in the past.

scholarly journals Separating damage from environmental effects affecting civil structures for near real-time damage detection

2017 ◽  
Vol 17 (4) ◽  
pp. 850-868 ◽  
Author(s):  
William Soo Lon Wah ◽  
Yung-Tsang Chen ◽  
Gethin Wyn Roberts ◽  
Ahmed Elamin
Keyword(s):  
Damage Detection ◽  
Real Time ◽  
Environmental Effects ◽  
Environmental Conditions ◽  
Principal Component ◽  
Detection Methods ◽  
Real Time Monitoring ◽  
Civil Structures ◽  
Data Set ◽  
Wide Range

Analyzing changes in vibration properties (e.g. natural frequencies) of structures as a result of damage has been heavily used by researchers for damage detection of civil structures. These changes, however, are not only caused by damage of the structural components, but they are also affected by the varying environmental conditions the structures are faced with, such as the temperature change, which limits the use of most damage detection methods presented in the literature that did not account for these effects. In this article, a damage detection method capable of distinguishing between the effects of damage and of the changing environmental conditions affecting damage sensitivity features is proposed. This method eliminates the need to form the baseline of the undamaged structure using damage sensitivity features obtained from a wide range of environmental conditions, as conventionally has been done, and utilizes features from two extreme and opposite environmental conditions as baselines. To allow near real-time monitoring, subsequent measurements are added one at a time to the baseline to create new data sets. Principal component analysis is then introduced for processing each data set so that patterns can be extracted and damage can be distinguished from environmental effects. The proposed method is tested using a two-dimensional truss structure and validated using measurements from the Z24 Bridge which was monitored for nearly a year, with damage scenarios applied to it near the end of the monitoring period. The results demonstrate the robustness of the proposed method for damage detection under changing environmental conditions. The method also works despite the nonlinear effects produced by environmental conditions on damage sensitivity features. Moreover, since each measurement is allowed to be analyzed one at a time, near real-time monitoring is possible. Damage progression can also be given from the method which makes it advantageous for damage evolution monitoring.

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