Resistance gene analogues are clustered on chromosome 3 of sugar beet and cosegregate with QTL for rhizomania resistance

Genome ◽  
2007 ◽  
Vol 50 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Jens Christoph Lein ◽  
Katrin Asbach ◽  
Yanyan Tian ◽  
Daniela Schulte ◽  
Chunyan Li ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Sugar Beet ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Chromosome 3 ◽  
Resistance Locus ◽  
Resistant Varieties ◽  
Rhizomania Resistance

Worldwide, rhizomania is the most important disease of sugar beet. The only way to control this disease is to use resistant varieties. Four full-length resistance gene analogues (RGAs) from sugar beet (cZR-1, cZR-3, cZR-7, and cZR-9) were used in this study. Their predicted polypeptides carry typical nucleotide-binding sites (NBSs) and leucin-rich repeat (LRR) regions, and share high homology to various plant virus resistance genes. Their corresponding alleles were cloned and sequenced from a rhizomania resistant genotype. The 4 RGAs were mapped as molecular markers, using sequence-specific primers to determine their linkage to the rhizomania resistance locus Rz1 in a population segregating for rhizomania resistance. One cZR-3 allele, named Rz-C, together with 5 other molecular markers, mapped to the Rz1 locus on chromosome 3 and cosegregated with quantitative trait loci for rhizomania resistance. After screening a bacterial artificial chromosome (BAC) library, 25 cZR-3-positive BACs were identified. Of these, 15 mapped within an interval of approximately 14 cM on chromosome 3, in clusters close to the Rz1 locus. Rz-C differentiates between susceptible and resistant beet varieties, and its transcripts could be detected in all rhizomania resistant varieties investigated. The potential of this RGA marker for cloning of rhizomania resistance genes is discussed.

Confirmation of some SCAR molecular markers linked to Rhizomania resistance gene (Rz1) in sugar beet

2017 ◽  
Vol 77 (2) ◽  
pp. 312
Author(s):  
Peyman Norouzi ◽  
Seyed Bagher Mahmoudi ◽  
Saeed Darabi ◽  
Mozhdeh Kakueinezhad
Keyword(s):  
Molecular Markers ◽  
Sugar Beet ◽  
Resistance Gene ◽  
Rhizomania Resistance

Efficiency of some molecular markers linked to rhizomania resistance gene (Rz 1 ) for marker assisted selection in sugar beet

2015 ◽  
Vol 18 (5) ◽  
pp. 319-323 ◽  
Author(s):  
Peyman Norouzi ◽  
Mohammad Sabzehzari ◽  
Hasan Zeinali
Keyword(s):  
Molecular Markers ◽  
Sugar Beet ◽  
Resistance Gene ◽  
Marker Assisted Selection ◽  
Rhizomania Resistance

QTL mapping of BNYVV resistance from the WB41 source in sugar beet

Genome ◽  
2005 ◽  
Vol 48 (2) ◽  
pp. 279-285 ◽  
Author(s):  
Sara Gidner ◽  
Britt-Louise Lennefors ◽  
Nils-Otto Nilsson ◽  
Jan Bensefelt ◽  
Evert Johansson ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Qtl Mapping ◽  
Resistance Gene ◽  
Beta Vulgaris ◽  
Incomplete Penetrance ◽  
Resistant Varieties ◽  
Major Resistance Gene ◽  
Rhizomania Resistance ◽  
Chromosome Iii ◽  
Greenhouse Tests

The most important rhizomania-resistance gene in sugar beet is the Rz1 gene from the Holly Sugar Company in California, the source widely used to breed partially resistant varieties. Other important gene sources are WB41 and WB42, which both originate from Beta vulgaris subsp. maritima collected in Denmark, and which have been reported to be similar. The major resistance gene in WB42 is known as Rz2. We studied the resistance in WB41 and used markers to map the major resistance gene in this source, which we call Rz3. It was identified on chromosome III. This is the chromosome that Rz1 and Rz2 have been mapped to. Data from greenhouse tests and ELISA showed that Rz3 had incomplete penetrance, with heterozygotes varying widely in resistance levels. The involvement of additional minor genes in the strong resistance of the original WB41 source cannot be excluded.Key words: BNYVV, WB41, rhizomania, QTL, Beta vulgaris, AFLP, SSR.

Bacterial artificial chromosome-derived molecular markers for early bolting in sugar beet

2005 ◽  
Vol 110 (6) ◽  
pp. 1027-1037 ◽  
Author(s):  
R. M. Gaafar ◽  
U. Hohmann ◽  
C. Jung
Keyword(s):  
Molecular Markers ◽  
Bacterial Artificial Chromosome ◽  
Sugar Beet ◽  
Artificial Chromosome

Physical mapping of resistant and susceptible soybean genomes near the soybean cyst nematode resistance gene Rhg4

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1057-1064 ◽  
Author(s):  
K S Lewers ◽  
S D Nilmalgoda ◽  
A L Warner ◽  
H T Knap ◽  
B F Matthews
Keyword(s):  
Resistance Gene ◽  
Soybean Cyst Nematode ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Nematode Resistance ◽  
Physical Distance ◽  
Cyst Nematode ◽  
Nematode Resistance Gene ◽  
Glycine Max L ◽  
Soybean Cyst Nematode Resistance

The soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is the foremost pest of soybean (Glycine max L. Merr.). The rhg1 allele on linkage group (LG) G and the Rhg4 allele on LG A2 are important in conditioning resistance. Markers closely linked to the Rhg4 locus were used previously to screen a library of bacterial artificial chromosome (BAC) clones from susceptible 'Williams 82' and identified a single 150-kb BAC, Gm_ISb001_056_G02 (56G2). End-sequenced subclones positioned onto a restriction map provided landmarks for identifying the corresponding region from a BAC library from accession PI 437654 with broad resistance to SCN. Seventy-three PI 437654 BACs were assigned to contigs based upon HindIII restriction fragment profiles. Four contigs represented the PI 437654 counterpart of the 'Williams 82' BAC, with PCR assays connecting these contigs. Some of the markers on the PI 437654 contigs are separated by a greater physical distance than in the 'Williams 82' BAC and some primers amplify bands from BACs in the mid-portion of the connected PI 437654 BAC contigs that are not amplified from the 'Williams 82' BAC. These observations suggest that there is an insertion in the PI 437654 genome relative to the 'Williams 82' genome in the Rhg4 region.Key words: BAC, deletion, insertion, resistance gene, soybean cyst nematode.

Mapping of rhizoctonia root rot resistance genes in sugar beet using pathogen response-related sequences as molecular markers

2008 ◽  
Vol 127 (6) ◽  
pp. 602-611 ◽  
Author(s):  
J. C. Lein ◽  
C. M. Sagstetter ◽  
D. Schulte ◽  
T. Thurau ◽  
M. Varrelmann ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Sugar Beet ◽  
Resistance Genes ◽  
Root Rot ◽  
Rhizoctonia Root Rot ◽  
Pathogen Response

Linkage mapping of powdery mildew and greenbug resistance genes on recombinant 1RS from 'Amigo' and 'Kavkaz' wheat–rye translocations of chromosome 1RS.1AL

Genome ◽  
2004 ◽  
Vol 47 (2) ◽  
pp. 292-298 ◽  
Author(s):  
Yehia Mater ◽  
Stephen Baenziger ◽  
Kulvinder Gill ◽  
Robert Graybosch ◽  
Lynda Whitcher ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Dna Sequences ◽  
Secale Cereale ◽  
Polyacrylamide Gel Electrophoresis ◽  
Rflp Markers ◽  
Resistance Locus ◽  
Greenbug Resistance

Cultivated rye (Secale cereale L., 2n = 2x = 14, RR) is an important source of genes for insect and disease resistance in wheat (Triticum aestivum L., 2n = 6x = 42). Rye chromosome arm 1RS of S. cereale 'Kavkaz' originally found as a 1BL.1RS translocation, carries genes for disease resistance (e.g., Lr26, Sr31, Yr9, and Pm8), while 1RS of the S. cereale 'Amigo' translocation (1RSA) carries a single resistance gene for greenbug (Schizaphis graminum Rondani) biotypes B and C and also carries additional disease-resistance genes. The purpose of this research was to identify individual plants that were recombinant in the homologous region of.1AL.1RSV and 1AL.1RSA using both molecular and phenotypic markers. Secale cereale 'Nekota' (1AL.1RSA) and S. cereale 'Pavon 76' (1AL.1RSV) were mated and the F1 was backcrossed to 'Nekota' (1AL.1AS) to generate eighty BC1F2:3 families (i.e., ('Nekota' 1AL.1RSA × 'Pavon 76' 1AL.1RSV) × 'Nekota' 1AL.1AS). These families were genotyped using the secalin–gliadin grain storage protein banding pattern generated with polyacrylamide gel electrophoresis to discriminate 1AL.1AS/1AL.1RS heterozygotes from the 1AL.1RSA+V and 1AL.1AS homozygotes. Segregation of the secalin locus and PCR markers based on the R173 family of rye specific repeated DNA sequences demonstrated the presence of recombinant 1AL.1RSA+V families. Powdery mildew (Blumeria graminis) and greenbug resistance genes on the recombinant 1RSA+V arm were mapped in relation to the Sec-1 locus, 2 additional protein bands, 3 SSRs, and 13 RFLP markers. The resultant linkage map of 1RS spanned 82.4 cM with marker order and spacing showing reasonable agreement with previous maps of 1RS. Fifteen markers lie within a region of 29.7 cM next to the centromere, yet corresponded to just 36% of the overall map length. The map position of the RFLP marker probe mwg68 was 10.9 cM distal to the Sec-1 locus and 7.8 cM proximal to the powdery mildew resistance locus. The greenbug resistance gene was located 2.7 cM proximal to the Sec-1 locus.Key words: microsatellites, SSRs, RFLP, secalin-gliadin, alien genes introgression.

scholarly journals Apple Contains Receptor-like Genes Homologous to the Cladosporium fulvum Resistance Gene Family of Tomato with a Cluster of Genes Cosegregating with Vf Apple Scab Resistance

2001 ◽  
Vol 14 (4) ◽  
pp. 508-515 ◽  
Author(s):  
Boris A. Vinatzer ◽  
Andrea Patocchi ◽  
Luca Gianfranceschi ◽  
Stefano Tartarini ◽  
Hong-Bin Zhang ◽  
...  
Keyword(s):  
Gene Family ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Transmembrane Domain ◽  
Venturia Inaequalis ◽  
Scab Resistance ◽  
Common Disease ◽  
Resistance Locus ◽  
Cladosporium Fulvum ◽  
High Quality

Scab caused by the fungal pathogen Venturia inaequalis is the most common disease of cultivated apple (Malus × domestica Borkh.). Monogenic resistance against scab is found in some small-fruited wild Malus species and has been used in apple breeding for scab resistance. Vf resistance of Malus floribunda 821 is the most widely used scab resistance source. Because breeding a high-quality cultivar in perennial fruit trees takes dozens of years, cloning disease resistance genes and using them in the transformation of high-quality apple varieties would be advantageous. We report the identification of a cluster of receptor-like genes with homology to the Cladosporium fulvum (Cf) resistance gene family of tomato on bacterial artificial chromosome clones derived from the Vf scab resistance locus. Three members of the cluster were sequenced completely. Similar to the Cf gene family of tomato, the deduced amino acid sequences coded by these genes contain an extracellular leucine-rich repeat domain and a transmembrane domain. The transcription of three members of the cluster was determined by reverse transcription-polymerase chain reaction to be constitutive, and the transcription and translation start of one member was verified by 5′ rapid amplification of cDNA ends. We discuss the parallels between Cf resistance of tomato and Vf resistance of apple and the possibility that one of the members of the gene cluster is the Vf gene. Cf homologs from other regions of the apple genome also were identified and are likely to present other scab resistance genes.

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