Specific cross-protective antigonococcal immunity in the murine genital tract

1984 ◽  
Vol 30 (4) ◽  
pp. 482-487 ◽  
Author(s):  
L. B. Corbeil ◽  
A. C. Wunderlich ◽  
J. M. Lyons ◽  
A. I. Braude
Keyword(s):  
Genital Tract ◽  
Practical Importance ◽  
Acquired Immunity ◽  
Igg Antibodies ◽  
Gonococcal Infection ◽  
Post Challenge ◽  
Igg Antibody ◽  
Heat Stable ◽  
The One ◽  
Heterologous Protection

Specific acquired immunity to gonococci was studied in systemically immunized mice, challenged with 107 gonococci by intrauterine inoculation. Protection after intraperitoneal immunization was monitored by vaginal cultures taken 24 h post-challenge, since events during the first 24 h postexposure to gonococci are crucial in determining the outcome of infection. Mice were protected against gonococcal challenge by two inoculations with either live or boiled gonococci given 4 weeks apart, whereas immunization with one inoculation did not protect against challenge 1 week later. Protection was correlated with high titers of IgG antibody in serum after two immunizations, but not with the high titers of serum IgM antibody found after the one immunization. IgG antibodies, but not IgM antibodies, were shown to pass into genital secretions. Protection could be passively transferred by serum with high titers of antibody. Of most practical importance was the finding that not only were heat-stable antigens protective, but also heterologous protection resulted after immunization with three strains differing in source (disseminated gonococcal infection versus gonorrhea), opacity–transparency characteristics, and serum sensitivity. The data indicate that IgG antibodies resulting from systemic immunization with heat-stable antigens may be able to provide cross-protection immunity against gonorrhea.

Seroprevalence of anti-SARS-CoV-2 IgG antibodies in hospitalized patients at a tertiary referral center in North India (Preprint)

2020 ◽  
Author(s):  
Dr. Animesh Ray ◽  
Dr. Komal Singh ◽  
Souvick Chattopadhyay ◽  
Farha Mehdi ◽  
Dr. Gaurav Batra ◽  
...  
Keyword(s):  
Tertiary Care ◽  
Age Groups ◽  
Hospitalized Patients ◽  
North India ◽  
P Value ◽  
Care Hospital ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Increased Risk ◽  
Significant Difference

BACKGROUND Seroprevalence of IgG antibodies against SARS-CoV-2 is an important tool to estimate the true extent of infection in a population. However, seroprevalence studies have been scarce in South East Asia including India, which, as of now, carries the third largest burden of confirmed cases in the world. The present study aimed to estimate the seroprevalence of anti-SARS-CoV-2 IgG antibody among hospitalized patients at one of the largest government hospital in India OBJECTIVE The primary objective of this study is to estimate the seroprevalence of SARS-CoV-2 antibody among patients admitted to the Medicine ward and ICU METHODS This cross-sectional study, conducted at a tertiary care hospital in North India, recruited consecutive patients who were negative for SARS-CoV-2 by RT-PCR or CB-NAAT. Anti-SARS-CoV-2 IgG antibody levels targeting recombinant spike receptor-binding domain (RBD) protein of SARS CoV-2 were estimated in serum sample by the ELISA method RESULTS A total of 212 hospitalized patients were recruited in the study with mean age (±SD) of 41.2 (±15.4) years and 55% male population. Positive serology against SARS CoV-2 was detected in 19.8%patients(95% CI 14.7-25.8). Residency in Delhi conferred a higher frequency of seropositivity 26.5% (95% CI 19.3-34.7) as compared to that of other states 8% (95% CI 3.0-16.4) with p-value 0.001. No particular age groups or socio-economic strata showed a higher proportion of seropositivity CONCLUSIONS Around, one-fifth of hospitalized patients, who were not diagnosed with COVID-19 before, demonstrated seropositivity against SARS-CoV-2. While there was no significant difference in the different age groups and socio-economic classes; residence in Delhi was associated with increased risk (relative risk of 3.62, 95% CI 1.59-8.21)

Immunization efficacy of ozone-exposed cercariae against subsequent Schistosoma mansoni challenge infection

2011 ◽  
Vol 61 (3) ◽  
pp. 289-301
Author(s):  
Azza H. Mohamed
Keyword(s):  
Schistosoma Mansoni ◽  
Worm Burden ◽  
Structural Changes ◽  
Relative Weight ◽  
Challenge Infection ◽  
Total Leukocyte Count ◽  
Post Challenge ◽  
Igg Antibody ◽  
Sem Images ◽  
Infected Control

AbstractCD1 mice were immunized subcutaneously with 20 ozone-exposed (70μg/ml, 1 minute exposure) Schistosoma mansoni cercariae weekly/three weeks. The efficacy of immunization was assessed 10 weeks post challenge infection by the determination of the worm burden, ova count, oogram, granuloma diameter, IgG reactions against soluble egg antigen (SEA) and tegument structural changes of recovered worms that are immunized. A reduced worm length and a reduction in worm burden were observed in the immunized group as compared to the infected not immunized group. Moreover, no ova were found in liver and intestine from the immunized mice as compared with infected control mice. Also, immunization with ozonated cercariae showed a decrement in the mean relative weight of liver and spleen. Total leukocyte count was increased in the immunized animal as compared to the infected control. The level of total IgG antibody against SEA decreased in immunized mice as compared with the infected control mice. Scanning electron microscope (SEM) images of worms recovered 10 weeks post challenge from the immunized group revealed extensive tegumental destruction. This study underlines the significant role of ozone attenuated cercariae vaccine against S. mansoni infection, which generated specific immunity with a significant level of protection.

The use of «coasorption» concept while describing the effect of coagulants on the wastewater aqua systems

2021 ◽  
pp. 33-39
Author(s):  
Е.В. Алексеев
Keyword(s):  
Wastewater Treatment ◽  
Industrial Wastewater ◽  
Practical Importance ◽  
Quantitative Relationship ◽  
Synthetic Dyes ◽  
Pollutant Concentrations ◽  
Wide Range ◽  
Complex Chemical Composition ◽  
The One ◽  
Raw Wastewater

Широкое распространение реагентного коагулирования в технологии очистки природных и промышленных сточных вод обусловлено, с одной стороны, сложностью химического состава и фазово-дисперсного состояния их аквасистем, с другой – многофакторностью действия на них коагулянтов. Рассмотрены основные механизмы взаимодействия коагулянтов и загрязняющих веществ на примере очистки промышленных сточных вод, содержащих органические вещества (поверхностно-активные вещества и синтетические красители). Физико-химическое понятие «коагуляция» отражает только один из механизмов действия коагулянтов на загрязнители. Принятые в настоящее время технологические показатели «доза коагулянта» и «удельная доза коагулянта» также не отражают многообразие взаимодействий загрязняющих веществ с солями коагулянтов и не привязаны к результату коагулирования. Это обусловливает трудность описания совокупного действия физико-химических процессов при добавлении коагулянтов в очищаемые воды и его количественной оценки. Решение проблемы возможно введением понятия «коасорбция», определяющего многофакторность взаимодействия коагулянтов с загрязняющими веществами в процессах очистки сточных вод коагулированием, и технологического показателя «удельная коасорбция», устанавливающего количественную взаимосвязь между величинами загрязняющих веществ в исходной и очищенной воде с дозой коагулянта. Графически представлены функции удельной коасорбции в форме изотерм коасорбции для двух типов поверхностно-активных веществ и синтетических красителей. На основании результатов анализа особенностей изотерм показано, что они отражают разные механизмы взаимодействия коагулянтов и загрязняющих веществ. Использование коасорбции как технологического понятия способствует выявлению механизмов взаимодействия загрязняющих веществ с коагулянтом и созданию наилучших условий для осуществления процесса коагулирования. Функциональное описание изотерм коасорбции позволяет экстраполировать результаты пробного коагулирования в широком интервале концентраций загрязняющих веществ. Практическое значение изотерм удельной коасорбции состоит в возможности определения доз реагентов при коагулировании воды по начальному содержанию загрязняющих веществ и требуемому в очищенных водах. The widespread use of chemical coagulation in purification of natural water and industrial wastewater is due, on the one hand, to the complex chemical composition and phase-disperse state of their aqua systems, and on the other hand, to the multifactorial effect of coagulants on them. The main mechanisms of interaction between coagulants and pollutants are considered through the example of industrial wastewater treatment containing organic substances (surfactants and synthetic dyes). The physicochemical term «coagulation» reflects only one of the mechanisms of the effect of coagulants on pollutants. The currently accepted process parameters «dose of coagulant» and «specific dose of coagulant» do not reflect the variety of interactions of pollutants with coagulant salts either, and are not linked to the result of coagulation. This makes it difficult to describe the collective effect of physicochemical processes while adding coagulants to the treated water and to quantify it. The solution to the problem is possible introducing the concept of «coasorption» that determines the multifactorial origin of the interaction of coagulants and pollutants in the processes of wastewater treatment by coagulation; and the process parameter «specific coasorption» that establishes a quantitative relationship between the concentrations of pollutants in raw wastewater and effluent with a dose of coagulant. The specific coasorption functions are graphically presented in the form of coasorption isotherms for two types of surfactants and synthetic dyes. Based on the results of the analysis of the features of the isotherms, it is shown that they reflect different mechanisms of interaction between coagulants and pollutants. The use of coasorption as a technological concept provides for identifying the mechanisms of interaction of pollutants with a coagulant and establishing the best conditions for the coagulation process. The functional description of the coasorption isotherms allows extrapolating the results of trial coagulation in a wide range of pollutant concentrations. The practical importance of specific coasorption isotherms provides for determining the dosages of chemicals during coagulation of water based on the initial concentration of pollutants and effluent standard.

scholarly journals Development and Validation of 13-plex Luminex-Based Assay for Measuring Human Serum Antibodies to Streptococcus pneumoniae Capsular Polysaccharides

mSphere ◽  
2018 ◽  
Vol 3 (4) ◽  
pp. e00128-18 ◽  
Author(s):  
Danka Pavliakova ◽  
Peter C. Giardina ◽  
Soraya Moghazeh ◽  
Shite Sebastian ◽  
Maya Koster ◽  
...  
Keyword(s):  
Human Serum ◽  
Lower Limit ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Correlate Of Protection ◽  
Immune Correlate ◽  
Relative Standard ◽  
Limit Of Quantitation

ABSTRACT A Luminex-based direct immunoassay (dLIA) platform has been developed to replace the standardized pneumococcal enzyme-linked immunosorbent assay platform. The multiplex dLIA simultaneously measures the concentration of serum immunoglobulin G (IgG) antibodies specific for pneumococcal capsular polysaccharide (PnPS) serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F. The assay uses poly-l-lysine (PLL)-conjugated PnPS, chemically coupled to spectrally distinct Luminex microspheres. Assay validation experiments were performed using residual human serum samples obtained from 13-valent pneumococcal conjugate vaccine (13vPnC) clinical studies. Assay results are expressed as IgG antibody concentrations in micrograms per milliliter using the international reference serum, 007sp. The lower limit of quantitation (LLOQ) for all serotypes covered in the 13-plex dLIA fell within the range of 0.002 to 0.038 µg/ml serum IgG. The difference between the lower limit and upper limit of the assay range was >500-fold for all serotypes, and assay variability was <20% relative standard deviation (RSD) for all serotypes. IgG antibody measurements were shown to be serotype-specific (some cross-reactivity was observed only between the structurally related serotypes 6A and 6B as well as 19A and 19F), and no interference was observed between the serotypes when the assay was performed in the 13-plex format compared to the singleplex assays. The 13-plex dLIA platform developed by Pfizer Inc. generates up to 143 test results in a single 96-well plate and is a suitable replacement of the enzyme-linked immunosorbent assay (ELISA) platform for evaluating vaccine clinical trials. IMPORTANCE The pneumococcal enzyme-linked immunosorbent assay (ELISA) measures IgG antibodies in human serum, and it is an important assay that supports licensure of pneumococcal vaccines. The immune correlate of protection, 0.35 µg/ml of IgG antibodies, was determined by the ELISA method. Pfizer has developed a new Luminex-based assay platform to replace the ELISA. These papers describe the important work of (i) validating the Luminex-based assay and (ii) bridging the immune correlate of protection (0.35 µg/ml IgG) to equivalent values reported by the Luminex platform.

scholarly journals Social Utopias and Engineering Design

2020 ◽  
Vol 3 (1) ◽  
pp. 570-583
Author(s):  
Waldemar Czajkowski
Keyword(s):  
Engineering Design ◽  
Practical Importance ◽  
The Other ◽  
Effective Solution ◽  
Possible World ◽  
Adequate Knowledge ◽  
Other Hand ◽  
Engineering Sciences ◽  
Global Problems ◽  
The One

AbstractA paradox of our time is identified: on the one hand – the development of one global system (ecological, technological and social), on the other hand – the still increasing “balkanization” of science. The dynamics of this systems is a source of well-known numerous global problems. Its possibly effective solution needs adequate knowledge about the system. For this reason, counteraction to “balkanization” of science is of great practical importance. And this counteraction should comprise not only development of “transboundary” sciences (such as biochemistry or social psychology) but also establishing and developing links between very distant disciplines. This text is intended as a contribution to linking social and engineering sciences. The notion of design plays the central role in this text. Its meaning in the engineering sciences. The notion of utopia has been chosen as a partial counterpart to the term of engineering design. This notion was defined using a concept of possible world – taken from modal logic. It encompasses two ideas: this of design and that of prediction, It is claimed that we need many utopias and that their plurality is of fundamental importance for protecting us against the threats of utopianism. The paper suggests that social utopias can play a heuristic role in engineering design (particularly in the initial phase of defining technological problems), and – on the other hand – that the theory of engineering design can be supportive for, badly needed, development of methodology of utopias creation.

scholarly journals Detection of antibodies to recombinant truncated flagellin and sonicated whole cell antigen of Burkholderia pseudomallei in acute melioidosis and in healthy Bangladeshi individuals

2020 ◽  
Vol 14 (1) ◽  
pp. 47-52
Author(s):  
Md Shariful Alam Jilani ◽  
Tang Thean Hock ◽  
Sraboni Mazumder ◽  
Fahmida Rahman ◽  
Md Mohiuddin ◽  
...  
Keyword(s):  
Rural Areas ◽  
Burkholderia Pseudomallei ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
Serum Samples ◽  
Igg Antibody ◽  
Whole Cell ◽  
Cell Antigen ◽  
The Mean

Background and objectives: Several types of Burkholderia pseudomallei antigens have been used to determine the antibody response in acute and asymptomatic cases. In the present study, we have detected immunoglobulin G (IgG) antibody to recombinant truncated flagellin antigen (RTFA) of B. pseudomallei in the sera of acute melioidosis cases and healthy individuals from melioidosis endemic areas of Bangladesh by indirect enzyme-linked immunosorbent assay (ELISA). In parallel, IgG antibody to sonicated whole cell antigen (SWCA) of B. pseudomallei was determined to compare with anti-RTFA antibody. Methodology: Serum samples from culture confirmed melioidosis cases and from healthy individuals aged 21 years and above residing in melioidosis endemic rural areas were included in the study. Serum IgG antibody to RTFA and SWCA of B. pseudomallei was determined by indirect ELISA. Results: Out of 8 culture confirmed acute melioidosis cases, 7 (87.5%) and 8 (100%) were positive for anti-B. pseudomallei IgG antibodies by RTFA and SWCA methods respectively. Among 361 healthy individuals, the rate of seropositivity by RTFA-ELISA was significantly less than that of SWCA-ELISA (16.1% versus 26.8%; p = 0.001). The mean optical density (OD) of RTFA-ELISA of positive cases was significantly less than that of SWCA-ELISA in both melioidosis and healthy individuals (0.79±0.11 versus 2.4±0.08, p = 0.0001; 0.67±0.01 versus 1.27±0.02, p = 0.0001). The sensitivity and specificity of RTFA-ELISA were 88.9% and 100% respectively. Conclusion: Findings of the study suggest that multiple or combination of antigens should be used to study the seroprevalence of B. pseudomallei infection in a community. Also, prospective study is necessary to find out the duration of persistence of antibodies to different antigenic components of B. pseudomallei after exposure. Ibrahim Med. Coll. J. 2020; 14(1): 47-52

scholarly journals Digitization of Law: Some Problematic Aspects

2019 ◽  
Vol 12 (5) ◽  
pp. 147
Author(s):  
Valeev Damir Khamitovich ◽  
Nuriev Anas Gaptraufovich
Keyword(s):  
Russian Federation ◽  
Practical Importance ◽  
Digital Economy ◽  
Russian Science ◽  
Resource Base ◽  
Legal Doctrine ◽  
Law And Development ◽  
The Russian Federation ◽  
Russian Law ◽  
The One

This article reveals the important practical importance of academic cooperation between legal doctrine and achievements of technical laboratories in terms of defining &ldquo;points of growth&rdquo; in questions of digitalization of law and development of legal tools aimed at regulating the technogenic factor on the one hand and legal support of &ldquo;game-changing&rdquo; results in a in the conditions of digital economy on the other hand. The important role of the transformation of social regulators, designed to regulate the &ldquo;infrastructural&rdquo; and &ldquo;institutional&rdquo; incorporation of &ldquo;digital&rdquo; technologies into the existing legal system, is noted. The current place of the Russian Federation on readiness for the digital economy is subject to, among other things, insufficient theoretical study as a result of the regulatory framework, which often does not act as a platform for growth, but rather contains many gaps - which have to be overcome at the expense of law enforcement practice. The article notes that the trend of &ldquo;digitalization&rdquo; of Russian law is closely linked to the need to maintain the ecosystem of the digital economy and to identify &ldquo;growth points&rdquo; and enforce their urgent character based on the state&rsquo;s resource base, defines a positive agenda for &ldquo;digitalization&rdquo; of Russian law and raises a number of questions for the Russian science. It is concluded that one of the topical issues in the framework of the &ldquo;digitalization&rdquo; of Russian law is legal robotics, which is perceived as the automation of workflows, the existence of interrelated algorithms of actions aimed at generating a predictable result based on some initial simulated and prescribed situation and maximum robotization of legal processes. Using the example of the Kazan Federal University, which proclaimed the promotion of innovative development of the focus areas of the Russian Federation as one of its missions, the achievements obtained as a result of the interaction of legal doctrine and technical laboratories are revealed.

Development of Dual Fluorescent Microsphere Immunological Assay for Detection of Pseudorabies Virus gE and gB IgG Antibodies

2020 ◽  
Author(s):  
chihai ji ◽  
Jingyu Wang ◽  
Yuchen Zeng ◽  
Haoming Pan ◽  
Yingfang Wei ◽  
...  
Keyword(s):  
Pseudorabies Virus ◽  
High Specificity ◽  
Antibody Detection ◽  
Economic Losses ◽  
Igg Antibodies ◽  
Wild Type ◽  
Swine Industry ◽  
Igg Antibody ◽  
Fluorescent Microsphere ◽  
Specificity And Sensitivity

Abstract Background Pseudorabies, also known as Aujezsky’s disease, is an acute viral infection caused by pseudorabies virus (PRV). Swine are one of the natural hosts of pseudorabies, therefore, the disease brings huge economic losses to the swine industry. Establishment of a differential diagnosis technique that can distinguish between wild-type infected and vaccinated pigs, and monitor vaccine-induced IgG is crucial for eventual eradication of pseudorabies.Results The aim of this study was to develop a rapid dual detection method for PRV gE and gB protein IgG antibodies with high specificity and sensitivity. PRV gE codons at amino acid residues (aa) 52–238 and gB codons at aa 539–741 were expressed to obtain recombinant PRV gE and gB proteins by pMAL-c5x vector. After purification with Qiagen Ni–NTA agarose affinity, the two proteins were analyzed by SDS-PAGE and immunoblotting assay. Two single fluorescent-microsphere immunoassays (FMIA) were established by coupling two recombinant proteins (gE and gB) with two magnetic microbeads and an effective dual FMIA was developed by integrating the two single assays. Optimal serum dilution for each assay, correlation with other common swine virus-positive sera and comparison with ELISA for two PRV antigens were tested for validation. Compared with ELISA, the specificity and sensitivity were 99.26% and 92.3% for gE IgG antibody detection and 95.74% and 96.3% for gB IgG antibody detection by dual-FMIA.Conclusion We provide a new method for monitoring PRV protective antibody in vaccinated pigs and differentiating wild-type-PRV-infected from vaccinated pigs

Neisseria gonorrhoeae

2020 ◽  
pp. 1025-1032
Author(s):  
Jackie Sherrard ◽  
Magnus Unemo
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Sexual Activity ◽  
Antimicrobial Therapy ◽  
Genital Tract ◽  
Systemic Disease ◽  
Gonococcal Infection ◽  
Gram Negative ◽  
Lower Genital Tract ◽  
Direct Inoculation ◽  
Sex With Men

Neisseria gonorrhoeae is a Gram-negative, intracellular diplococcus that is transmitted by direct inoculation of infected secretion from one mucosa to another. It primarily colonizes the columnar epithelium of lower genital tract, only occasionally spreading to the upper genital tract or causing systemic disease. Oropharyngeal and rectal infections are common in men who have sex with men but also occur in women. N. gonorrhoeae is almost exclusively transmitted by sexual activity. Oropharyngeal and rectal infections usually produce no symptoms; disseminated gonococcal infection is a comparatively benign bacteraemia affecting joints (particularly shoulder and knee) and skin; traditionally more common in women than men. The gonococcus has adapted rapidly to prevalent antimicrobial usage, leading to resistance to all antibiotics used for treatment, notably penicillins, fluoroquinolones, macrolides, tetracycline, and cephalosporins. This development has resulted in major concerns internationally and the introduction of international and national action/response plans as well as dual antimicrobial therapy.

scholarly journals Kinetics of IgG Antibodies in Previous Cases of Dengue Fever—A Longitudinal Serological Survey

2020 ◽  
Vol 17 (18) ◽  
pp. 6580
Author(s):  
Qilin Wu ◽  
Qinlong Jing ◽  
Xiujuan Wang ◽  
Lili Yang ◽  
Yilan Li ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Antibody Level ◽  
Southern China ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Related Factors ◽  
Factors Affecting ◽  
Serological Investigation ◽  
Significant Difference

Guangzhou is believed to be the most important epicenter of dengue outbreaks in southern China. In this study, a longitudinal serological investigation of previous cases of dengue fever in Guangzhou was conducted to explore the persistence of IgG antibodies and related factors affecting the changes of antibody level. We recruited 70 dengue virus type 1 (DENV-1) primary infection cases at two years post infection for serological investigation and conducted a second follow-up in the 5th year of prognosis. An enzyme-linked immunosorbent assay (ELISA) for DENV IgG antibody was examined in all study subjects. Potential factors associated with the concentration of serum total IgG antibody were determined by the generalized estimation equation (GEE). No significant difference in serum total IgG antibody positive rate between two follow-ups was observed (χ2 = 3.066, p = 0.080). However, there was a significant difference in the concentration of serum total IgG antibody between the two follow-ups (Z = 7.154, p < 0.001). The GEE showed that the antibody level in the five-year prognosis was mainly affected by the antibody level in the two-year prognosis (OR: 1.007, 95%CI: 1.005–1.009). In conclusion, the serum IgG antibodies of previous dengue fever cases can persist for a long time.

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