Structural elucidation of the capsular polysaccharide of Neisseriameningitidis group H

1984 ◽  
Vol 62 (8) ◽  
pp. 1519-1524 ◽  
Author(s):  
Francis Michon ◽  
René Roy ◽  
Harold J. Jennings ◽  
Fraser E. Ashton
Keyword(s):  
Capsular Polysaccharide ◽  
Specific Antigen ◽  
Structural Elucidation ◽  
Major Group ◽  
Molar Ratio ◽  
Substitution Pattern ◽  
Specific Determinant ◽  
Unit Formula

The capsular polysaccharide from newly isolated Neisseriameningitidis group H elaborates a structurally unique polysaccharide which is the group specific antigen. The polysaccharide contains D-galactose, glycerol, and phosphate in the molar ratio of 1:1:1 and is composed of the following basic repeating unit:[Formula: see text]The polysaccharide contains O-acetyl groups, in the molar ratio of 0.8:1.0 with D-galactose, which are essential to the formation of the major group specific determinant based on serological experiments described. Although all the O-acetyl groups are located on D-galactopyranosyl residues, the substitution pattern is complex, 60% of the residues being substituted at O-3 and 20% at O-2.

Structural elucidation of the capsular polysaccharide of Streptococcus pneumoniae type 9N

1983 ◽  
Vol 61 (10) ◽  
pp. 1102-1107 ◽  
Author(s):  
Karl-Gunnar Rosell ◽  
Harold J. Jennings
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Magnetic Resonance ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Cross Reactivity ◽  
Structural Elucidation ◽  
Molar Ratio ◽  
The Cross ◽  
Nuclear Magnetic

The specific capsular polysaccharide of Streptococcus pneumoniae type 9N (American type 9) contains D-glucose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-mannose in the molar ratio of 2:1:1:1. Accumulated data from spectroscopic (13C and 1H nuclear magnetic resonance) and methylation analyses of the native and specifically degraded polysaccharide indicated that it was linear and composed of the following pentasaccharide repeating unit; —4)-α-D-GlcpA-(1 → 3)-α-D-Glcp-(1 → 3)-β-D-ManpNAc-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-GlcpNAc(1 →. Structural regions in the type 9N polysaccharide common to those of types 9A, 9L, and 9V have been identified which account for the cross-reactivity of this groups of polysaccharides.

Structure of the capsular polysaccharide antigen of type IV group B Streptococcus

1989 ◽  
Vol 67 (5) ◽  
pp. 877-882 ◽  
Author(s):  
Jose L. Di Fabio ◽  
Francis Michon ◽  
Jean-Robert Brisson ◽  
Harold J. Jennings ◽  
Michael R. Wessels ◽  
...  
Keyword(s):  
Capsular Polysaccharide ◽  
Group B Streptococcus ◽  
Molar Ratio ◽  
Partial Hydrolysis ◽  
Spectroscopic Techniques ◽  
Polysaccharide Antigen ◽  
Type Iv ◽  
High Resolution Nmr ◽  
Group B ◽  
Unit Formula

The native polysaccharide antigen isolated from type IV group B Streptococcus contains D-galactose, D-glucose, 2-acetamido-2-deoxy-D-glucose, and sialic acid in the molar ratio 2:2:1:1 and is composed of the following repeating unit:[Formula: see text]The structural analysis of this antigen was based on results obtained from methylation analysis, partial hydrolysis, nitrous acid deamination, and nuclear magnetic resonance spectroscopic techniques. Keywords: capsular polysaccharide, high resolution NMR spectroscopy, group B Streptococcus, polysaccharide structure, polysaccharide degradation.

Purification and characterization of prostate-specific antigen (PSA) complexed to alpha 1-antichymotrypsin: potential reference material for international standardization of PSA immunoassays

1995 ◽  
Vol 41 (9) ◽  
pp. 1273-1282 ◽  
Author(s):  
Z Chen ◽  
A Prestigiacomo ◽  
T A Stamey
Keyword(s):  
Molecular Mass ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Gel Filtration ◽  
Exchange Chromatography ◽  
Molar Ratio ◽  
Free Psa ◽  
Apparent Homogeneity ◽  
International Standardization

Abstract We describe for the first time a protocol to purify to apparent homogeneity an in vitro-prepared complex of prostate-specific antigen (PSA) and alpha 1-antichymotrypsin (ACT) by using a combination of gel filtration and ion-exchange chromatography. The purity of the PSA-ACT complex was confirmed by gel electrophoresis and Western blot. The PSA-ACT complex was stable in the pH range 6.0 to 7.8; it was also stable in various matrices, temperatures, and high concentrations of salt. Purification of the PSA-ACT complex was highly reproducible. An absorptivity of 0.99 L x g-1 x cm-1 at 280 nm was assigned to the PSA-ACT complex, based on amino acid analysis. Because PSA and ACT bind in a 1:1 molar ratio, we determined the molecular mass of the PSA-ACT complex as the mass encoded by the cDNA of ACT (plus 26% carbohydrate) plus the molecular mass of PSA (28,430 Da), which totals 89,280 Da. Using this material, we made two common calibrators, one of 100% PSA-ACT complex and one of 90% PSA-ACT complex plus 10% free PSA by volume (90:10 calibrator). Substitution of these calibrators for the manufacturers' calibrators in nine commercial immunoassays substantially reduced differences between immunoassays, especially for serum PSA values between 4 and 10 micrograms/L. The 90:10 calibrator is recommended as a universal calibrator for international standardization of PSA immunoassays.

scholarly journals Complete NMR Structural Elucidation of the Capsular Polysaccharide Adjuvant from Klebsiella I-714

1996 ◽  
Vol 241 (2) ◽  
pp. 602-610 ◽  
Author(s):  
Olivier Adam ◽  
Michel Riviere ◽  
Alain Vercellone ◽  
Pierre F. Monsan ◽  
Germain Puzo
Keyword(s):  
Capsular Polysaccharide ◽  
Structural Elucidation

Structural elucidation of the capsular polysaccharide of Bacteroides fragilis strain 23745M1

1995 ◽  
Vol 275 (2) ◽  
pp. 333-341 ◽  
Author(s):  
Viliam Pavliak ◽  
Dušan Uhrín ◽  
Jean-Robert Brisson ◽  
Arthur O. Tzianabos ◽  
Dennis L. Kasper ◽  
...  
Keyword(s):  
Capsular Polysaccharide ◽  
Bacteroides Fragilis ◽  
Structural Elucidation

Structure of the Capsular Polysaccharide of Klebsiella K-type 20

1973 ◽  
Vol 51 (18) ◽  
pp. 3015-3020 ◽  
Author(s):  
Yuen-Min Choy ◽  
Guy G. S. Dutton
Keyword(s):  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Acetyl Group ◽  
Unit Formula

Methylation, periodate oxidation, and partial hydrolysis studies on the capsular polysaccharide, and on the carboxyl reduced polymer, of Klebsiella K20 show the structure to consist of a repeating unit[Formula: see text]The anomeric linkages were determined by p.m.r. spectroscopy of the carboxyl reduced polysaccharide, and periodate degraded polysaccharides. The p.m.r. spectroscopy of the original polysaccharide also showed the presence in the polysaccharide of one O-acetyl group per eight sugar residues.

Structural elucidation of the novel type VII group B Streptococcus capsular polysaccharide by high resolution NMR spectroscopy

1995 ◽  
Vol 277 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Grigorij Kogan ◽  
Jean-Robert Brisson ◽  
Dennis L. Kasper ◽  
Christina von Hunolstein ◽  
Graziella Orefici ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
High Resolution ◽  
Capsular Polysaccharide ◽  
Group B Streptococcus ◽  
Structural Elucidation ◽  
The Novel ◽  
High Resolution Nmr ◽  
Group B
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