THE SIGNIFICANCE OF THE NUMBER OF PRECIPITIN BANDS IN AGAR GEL MEDIUM FOR THE DETERMINATION OF THE COMPLEXITY OF ANTIGENS

M. Richter; B. Rose; A. H. Sehon

doi:10.1139/y58-119

THE SIGNIFICANCE OF THE NUMBER OF PRECIPITIN BANDS IN AGAR GEL MEDIUM FOR THE DETERMINATION OF THE COMPLEXITY OF ANTIGENS

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y58-119 ◽

1958 ◽

Vol 36 (1) ◽

pp. 1105-1113

Author(s):

M. Richter ◽

B. Rose ◽

A. H. Sehon

Keyword(s):

Liquid Medium ◽

Agar Medium ◽

Model System ◽

Aminobenzoic Acid ◽

Liquid Media ◽

Agar Gel ◽

Precipitin Test ◽

Gel Technique ◽

New Interpretation

A new interpretation is presented to account for inconsistancies which may arise when comparing data obtained with antibody–antigen systems in agar and in liquid media. For a model system, different haptens (p-aminobenzoic acid and p-sulphanilic acid) coupled to proteins were used. It has been demonstrated that the number of precipitin bands formed in agar medium by these antibody–antigen systems may be smaller than the number of optimal proportion zones detected for these systems in liquid medium, provided the different haptens are coupled to the same carrier. This effect is not due to a lesser sensitivity of the reaction in agar but rather to the inherent limitations of this method. Thus, by the agar technique, not more than one antigenic moiety (present on a molecule) is detected, whereas by the precipitin test in liquid medium optimal proportion zones with respect to each of the antigenic moieties (on the molecule) may be detected. This limitation in agar is not considered to detract from the value of the agar gel technique when it is used for the detection of independent antigens.

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THE SIGNIFICANCE OF THE NUMBER OF PRECIPITIN BANDS IN AGAR GEL MEDIUM FOR THE DETERMINATION OF THE COMPLEXITY OF ANTIGENS

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o58-119 ◽

1958 ◽

Vol 36 (11) ◽

pp. 1105-1113 ◽

Cited By ~ 5

Author(s):

M. Richter ◽

B. Rose ◽

A. H. Sehon

Keyword(s):

Liquid Medium ◽

Agar Medium ◽

Model System ◽

Aminobenzoic Acid ◽

Liquid Media ◽

Agar Gel ◽

Precipitin Test ◽

Gel Technique ◽

New Interpretation

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Enumeration of petroleum-degrading marine and estuarine microorganisms by the most probable number method

Canadian Journal of Microbiology ◽

10.1139/m78-089 ◽

1978 ◽

Vol 24 (5) ◽

pp. 552-557 ◽

Cited By ~ 94

Author(s):

A. L. Mills ◽

C. Breuil ◽

R. R. Colwell

Keyword(s):

Crude Oil ◽

Liquid Medium ◽

Silica Gel ◽

Agar Medium ◽

Most Probable Number ◽

Probable Number ◽

Petroleum Degraders

Several media designed for use in a most probable number (MPN) determination of petroleum-degrading microorganisms were compared. The best results, i.e., largest numbers, were obtained using a buffered (32 mM PO4≡) liquid medium containing 1% hydrocarbon substrate. Of 104 presumptive oil degraders tested, 20 grew on oil agar medium but did not utilize oil or a mixture of pure paraffinic hydrocarbons (C10 to C16n-alkanes) in liquid (MPN) medium. Visible turbidity in the liquid medium was correlated with hydrocarbon utilization. Counts of petroleum degraders obtained using liquid medium (MPN) were in most cases higher than those obtained on an oil-amended silica gel medium. Both procedures yield an estimation of oil degraders, and the oil-amended agar permits growth of organisms which do not degrade crude oil. All strains of oil-degrading microorganisms examined in this study were lipolytic, but the converse was not always true.

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Prevention of shoot vitrification of strawberry micropropagated shoots proliferated on liquid media by new antivitrifying agents

Canadian Journal of Plant Science ◽

10.4141/cjps93-037 ◽

1993 ◽

Vol 73 (1) ◽

pp. 231-235 ◽

Cited By ~ 4

Author(s):

Chafik Hdider ◽

Yves Desjardins

Keyword(s):

Liquid Medium ◽

Key Words ◽

Agar Medium ◽

High Rate ◽

Fragaria Ananassa ◽

Liquid Media ◽

Cellulose Plugs

Micropropagated strawberry (Fragaria × ananassa Duch. ’Kent.’) cultured for 4 wk in liquid proliferation media supported on cellulose plugs (sorbarods) developed a high rate of vitrification. This high percentage of abnormal plantlets was significantly reduced by the inclusion of two antivitrifying agents, EM1 and EM2, to the liquid medium. Virtually no vitrification occurred at 5 g L−1of EM1 or EM2. Shoots multiplied on liquid medium supplemented with 5 g L−1 of either EM1 or EM2 had significantly lower fresh weights and lower multiplication rates than on solidified agar medium (0.7%). EM2 produced better growth and proliferation than EM1 or liquid medium alone and was easier to use than EM1. Key words: Vitrification, antivitrifying agent, liquid medium

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Simultaneous Spectrophotometric Determination of Salbutamol by Coupling with Diazotized 4-Aminobenzoic acid

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.v8i1.8435 ◽

2017 ◽

Vol 8 (1) ◽

Author(s):

Hind Hadi ◽

Gufran Salim

Keyword(s):

Pharmaceutical Preparations ◽

Coupling Reaction ◽

Dosage Forms ◽

Water Soluble ◽

Trace Determination ◽

Aminobenzoic Acid ◽

Optimum Conditions ◽

Colored Product ◽

Versus Concentration

A simple, rapid and sensitive spectrophotmetric method for trace determination of salbutamol (SAL) in aqueous solution and in pharmaceutical preparations is described. The method is based on the diazotization coupling reaction of the intended compound with 4-amino benzoic acid (ABA) in alkaline medium to form an intense orange, water soluble dye that is stable and shows maximum absorption at 410 nm. A graph of absorbance versus concentration indicates that Beer’s law is obeyed over the concentration range of 0.5-30 ppm, with a molar absorbtivity 3.76×104 L.mol-1 .cm-1 depending on the concentration of SAL. The optimum conditions and stability of the colored product have been investigated and the method was applied successfully to the determination of SAL in dosage forms.

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