Complex population differentiation in checkerspot butterflies (Euphydryas spp.)

1989 ◽  
Vol 67 (2) ◽  
pp. 330-335 ◽  
Author(s):  
Peter F. Brussard ◽  
John F. Baughman ◽  
Dennis D. Murphy ◽  
Paul R. Ehrlich ◽  
Janet Wright
Keyword(s):  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Genetic Distances ◽  
Sympatric Populations ◽  
Separate Species ◽  
Euphydryas Anicia ◽  
Complex Population ◽  
Variable Species ◽  
Checkerspot Butterflies ◽  
Euphydryas Chalcedona

Gel electrophoresis was used to obtain an estimate of the genetic similarity among populations of Euphydryas anicia, Euphydryas chalcedona, and Euphydryas colon in an effort to determine whether they should be considered separate species or arbitrary groupings of populations in a single polytypic species. The average genetic distances between these entities, which collectively are referred to as the "ACC complex," are of the magnitude found between insect subspecies rather than between insect species. Furthermore, these entities show no unique combinations of morphological or biochemical characters that permit all individuals to be assigned with assurance to a specific taxon. Although cases of apparent reproductive isolation between some sympatric populations within the ACC complex have been reported, they appear to be terminal populations of "rings of races," rather than points of contact between completely isolated reproductive units. We recommend that the ACC complex be treated as a single highly variable species, Euphydryas chalcedona.

GENETIC RELATIONSHIPS AMONG REPRESENTATIVE POPULATIONS OF FIVE CHORISTONEURA SPECIES: C. OCCIDENTALIS, C. RETINIANA, C. BIENNIS, C. LAMBERTIANA, AND C. FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)

1981 ◽  
Vol 113 (9) ◽  
pp. 857-865 ◽  
Author(s):  
M. W. Stock ◽  
P. J. Castrovillo
Keyword(s):  
British Columbia ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Interspecific Variation ◽  
Genetic Distances ◽  
Starch Gel Electrophoresis ◽  
Separate Species ◽  
Individual Gene ◽  
Starch Gel

AbstractThe genetic make-up of representative populations of five Choristoneura species was compared using starch gel electrophoresis. Species included C. occidentalis Freeman from Idaho, C. biennis Freeman from British Columbia, C. retiniana (Walsingham) (= C. viridis Freeman) from Oregon, C. lambertiana ponderosana Obraztsov from Colorado, and C. fumiferana (Clemens) from Maine. When variation at individual gene loci was examined, intraspecific variation was often as great, and sometimes greater, than interspecific variation and few significant differences were noted among the species. The highest levels of overall genetic similarity occurred among C. occidentalis, C. biennis, and C. retiniana. Relatively greater genetic distances were found between this group and C. lambertiana and C. fumiferana. C. fumiferana was most distantly related to all other groups. Genetic identity values fell within the range more commonly associated with conspecific populations rather than with separate species.

Intraspecific genetic variability of isozymes in the ectomycorrhizal fungus Suillus tomentosus

1988 ◽  
Vol 66 (3) ◽  
pp. 588-594 ◽  
Author(s):  
Hong Zhu ◽  
Kenneth O. Higginbotham ◽  
Bruce P. Dancik ◽  
Stan Navratil
Keyword(s):  
Genetic Variability ◽  
Host Selection ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Ectomycorrhizal Fungus ◽  
Habitat Isolation ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Forest Regions ◽  
Starch Gel

Mycelial extracts of 43 isolates of Suillus tomentosus (Kauffm.) Singer, Snell & Dick collected from four boreal forest regions in Alberta were subjected to starch gel electrophoresis. A total of 21 bands was resolved from eight different enzyme systems presumably representing 13 loci. Six loci were polymorphic among these isolates. Cluster and principal components analyses demonstrated that intraspecific genetic variability of this fungus existed among and within forest regions. Polymorphic loci of acid phosphatase and alkaline phosphatase exhibited the greatest genetic similarity among the isolates within forest regions. Habitat isolation and host selection could be the major sources of genetic variation among forest regions.

scholarly journals Genetic diversity of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by SSR markers

2014 ◽  
Vol 66 (4) ◽  
pp. 1485-1492 ◽  
Author(s):  
Aleksandar Lucic ◽  
Vladan Popovic ◽  
Marija Nevenic ◽  
Danijela Ristic ◽  
Ljubinko Rakonjac ◽  
...  
Keyword(s):  
Pinus Sylvestris ◽  
Ssr Markers ◽  
Scots Pine ◽  
Correspondence Analysis ◽  
Genetic Similarity ◽  
Genetic Distances ◽  
Spatial Distance ◽  
Population Genetic Differentiation ◽  
Ssr Analysis ◽  
Interpopulation Variability

This paper presents the results of analysis of the genetic variability of seven Scots pine (Pinus sylvestris L.) populations in Serbia using SSR markers. Genomic DNA was isolated from seed tissue of all seven populations. The concentration of DNA samples was within the range of 1-4 mg/ml. Different PCR protocols were used depending on the type of SSR markers. The total number of fragments obtained by SSR analysis with 4 selected primers was 17 (only bands of strong and medium intensity were considered), of which 6 fragments were polymorphic (35.29%). In order to analyze the genetic similarity of the analyzed populations, graphs of correspondence analysis and UPGMA clusters were produced. By comparative analysis of the obtained dendrograms, the dependence of population genetic differentiation and spatial distance was observed, i.e. their isolation by natural barriers. The results indicate that in further research of interpopulation variability it is necessary, when graphically interpreting genetic distances, to use both methods of statistical analysis (UPGMA analysis and correspondence analysis).

scholarly journals EVALUATION OF GENETIC DIVERSITY IN CACAO COLLECTED FROM KOLAKA, SOUTHEAST SULAWESI, USING SSR MARKERS

2016 ◽  
Vol 16 (2) ◽  
pp. 71
Author(s):  
Rubiyo Rubiyo ◽  
Nur Kholilatul Izzah ◽  
Indah Sulistiyorini ◽  
Cici Tresniawati
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Similarity Coefficient ◽  
Breeding Program ◽  
New Variety ◽  
Average Value ◽  
The Third ◽  
Ctab Method

Kolaka, which is located in Southeast Sulawesi, has long been known as one of cacao production centers in Indonesia. Therefore, many different cacao germplasms can be found in this region. The study aimed to evaluate genetic diversity and relationships of 12 cacao genotypes collected from Kolaka. Genomic DNA was extracted by using a modified CTAB method. Meanwhile, genetic diversity was analyzed based on 16 SSR markers, which then separated by 6% non-denaturing polyacryl-amide gel electrophoresis. The result showed that all of those markers, 14 markers exhibited polymorphism and subsequently used for data analysis using NTSYS and PowerMarker program. About 70 different alleles were generated from 12 cacao genotypes analyzed with an average of 5 alleles per locus. Average value of polymorphism information content (PIC) resulted in this study was 0.59. The cluster analysis using UPGMA method based on the genetic similarity coefficient revealed that all cacao genotypes were separated into three major groups. The first group consisted of five cacao genotypes, the second one held four cacao genotypes, whereas the third group contained three genotypes. This result indicates that three genotypes that clustered separately from the others could be used as a good clonal candidate for cacao breeding program. The information resulted from this present study would be useful for future cacao breeding program, especially in efforts to release a new variety.

scholarly journals Determining the degree of kinship of sarlik yaks bred in the mountainous regions of the Kabardino-Balkarian Republic by blood groups

2021 ◽  
Vol 262 ◽  
pp. 02014
Author(s):  
Lyudmila Sabanchieva ◽  
Kristina Kanukova ◽  
Ismail Gazaev ◽  
Murat Gubzhokov ◽  
Zalina Bogotova
Keyword(s):  
Genetic Similarity ◽  
Structural Changes ◽  
Animal Husbandry ◽  
Genetic Distances ◽  
Blood Groups ◽  
Animal Breeding ◽  
Frequency Of Occurrence ◽  
Antigenic Composition ◽  
Mountainous Regions ◽  
Breeding Work

The formation of immunogenetics as a science was the result of numerous studies on the study of blood groups and other polymorphic systems, the results of which are successfully applied both in animal husbandry and in animal breeding. Modern biotechnological methods used to analyze blood groups enable us to determine the true origin of animals. To determine the correctness of the breeding processes occurring in the breed, the antigenic composition of blood groups, which are also used to study the reliability of origin, needs to be studied. The results obtained make it possible to analyze genetic similarity, determine the level of polymorphism and the nature of structural changes in the result of breeding work. Also, by analyzing the distribution of the frequency of occurrence of antigens, it is possible to determine the genetic distances and genetic similarity of breeds and lines among themselves [1].

scholarly journals Генетический полиморфизм редких и малораспространенных аборигенных донских генотипов L

2019 ◽  
pp. 191-197 ◽  
Author(s):  
Elena Ilnitskaya ◽  
Lyudmila Naumova ◽  
Valentina Ganich ◽  
Sergey Tokmakov ◽  
Marina Makarkina
Keyword(s):  
Vitis Vinifera ◽  
Genetic Similarity ◽  
Genetic Distances ◽  
The Other ◽  
Vitis Vinifera L ◽  
Separate Branch ◽  
History Of ◽  
The Difference ◽  
Dna Profiles ◽  
Basic Minimum

История виноградарства на Дону насчитывает несколько веков, местные сорта винограда многообразны и специфичны. Микросателлитные маркеры широко используются для генотипирования сортов и подвоев винограда, при изучении происхождения сортов и анализе их родословной. Целью исследования было изучение выборки редких и малораспространенных автохтонных донских сортов и сравнение их с другими аборигенными донскими генотипами на основе данных ДНК-анализа. В исследования включены 23 стародавних донских сорта. Генотипирование проводили методом микросателлитного профилирования. В исследовании использовали микросателлитные маркеры (SSR), рекомендованные в качестве основного минимального набора для ДНК-паспортизации сортов вида Vitis vinifera L.: VVMD5, VVMD7, VVMD27, VVS2, VrZAG62 и VrZAG79. По результатам проведенного анализа все изученные образцы показали сорт-специфическую комбинацию аллелей в идентифицированных ДНК-профилях. Количество выявленных аллелей составило в среднем 8 аллелей/локус. Наибольший полиморфизм в исследовании этой группы донских сортов был обнаружен в локусе VVMD5: идентифицировано 10 аллелей на локус, наименьшее - в локусе VrZAG62: 6 аллелей/локус. Основываясь на данных SSR-анализа, степень генетического сходства сортов оценивали с использованием метода UPGMA. Кластерный анализ матрицы генетических дистанций, созданный на основе выявленных значений аллелей в шести микросателлитных локусах исследуемых сортов, определил несколько групп генотипов. Сорт Красностоп золотовский выделился в отдельную ветвь, что указывает на различия между этим генотипом и другими сортами исследуемой выборки. Наивысший уровень генетического сходства наблюдался между следующими парами сортов: Крестовский и Бургундский, Шилохвостый и Мушкетный, Кумшацкий черный и Ефремовский.The history of viticulture on the Don goes back several centuries. Local grapevine varieties are diverse and peculiar. Microsatellite markers are widely used in genotyping grapevine cultivars and rootstocks, in grapevine origin and breeding background analysis. Our study aimed to examine samples of rare and less common autochthonous Don varieties, and compare them with the other aboriginal Don genotypes using DNA data. The study involved 23 traditional Don varieties. The genotyping was done by microsatellite profiling. The study used microsatellite (SSR) markers recommended as the basic minimum set for DNA-certification of the genotypes of Vitis vinifera L.: VVMD5, VVMD7, VVMD27, VVS2, VrZAG62 and VrZAG79. Based on the findings, all the studied samples demonstrated variety-specific combination of alleles in the identified DNA profiles. The number of detected alleles on average was 8 alleles/locus. The greatest polymorphism in the studied group of Don varieties was detected in VVMD5 locus: 10 alleles per locus were identified, the smallest in VrZAG62 locus: 6 alleles/locus. UPGMA method was used to assess the extent of genetic similarity of the varieties based on SSR-genotyping data. Based on determined allele values of the studied varieties, cluster analysis of the genetic distances matrix determined several groups of genotypes. ‘Krasnostop zolotovskiy’ variety stood out as a separate branch, which indicates the difference between this genotype and the other varieties of the studied sampling. The highest level of genetic similarity was observed between the following pairs of varieties: ‘Krestovskiy’ and ‘Burgundskiy’, ‘Shilohvostyi’ and ‘Mushketnyi’, ‘Kumshatskiy chornyi’ and ‘Efremovskiy’.

The use of random amplified microsatellite polymorphic DNA and coefficients of parentage to determine genetic relationships in barley

Genome ◽  
1998 ◽  
Vol 41 (4) ◽  
pp. 477-486 ◽  
Author(s):  
J A Dávila ◽  
M P Sánchez de la Hoz ◽  
Y Loarce ◽  
E Ferrer
Keyword(s):  
Hordeum Vulgare ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Spring Barley ◽  
Genetic Distances ◽  
Mantel Test ◽  
Winter Barley ◽  
Mean Values ◽  
Simple Sequence ◽  
Hordeum Vulgare Ssp

Seventy European barley lines (Hordeum vulgare ssp. vulgare) and 29 Hordeum vulgare ssp. spontaneum accessions were evaluated for random amplified microsatellite polymorphism (RAMP). PCR was performed with 5'-anchored primers complementary to microsatellites in combination with random primers. Of 20 primers assayed in barley, only 9 produced well-resolved fragment patterns in H. vulgare ssp. spontaneum. On the basis of 56 polymorphic fragments, genetic distances between the two subspecies were calculated. Barley samples were subdivided according to growth habit and spike morphology. The smallest genetic distance was found between winter cultivars and accessions of H. vulgare ssp. spontaneum. The 20 primers assayed in the barley lines produced 140 polymorphic fragments that were used to calculate genetic similarity between lines. Mean genetic similarity within groups of lines ranged from 0.693 for 6-rowed winter barley to 0.657 for 6-rowed spring barley. Within these groups, mean values were significantly higher than mean genetic similarity between groups. Principal-coordinate analysis clearly separated the 2-rowed spring and 6-rowed winter types. Cluster analysis of spring and winter types showed subclustering consistent with the available pedigrees. Coefficients of parentage (COPs) were calculated for 29 spring and 20 winter lines. Mean values of 0.0741 for spring barley and 0.0458 for winter barley were obtained. RAMP-based genetic similarity matrices were compared with the corresponding COP-based matrices by the Mantel test. The correlation between them was poor (r = 0.21, P < 0.05), indicating little relationship between these two estimators of genetic similarity. The relative influence of factors involved in the calculation of these two estimators is discussed.Key words: genetic diversity, microsatellites, simple sequence repeats, fingerprinting, barley.

Electrophoretic characterization of Solomon Islands populations of Nyctimene and Rousettus (Chiroptera: Pteropodidae).

2003 ◽  
Vol 25 (1) ◽  
pp. 41 ◽  
Author(s):  
JM Macaranas ◽  
D Colgan ◽  
S Ingleby
Keyword(s):  
Papua New Guinea ◽  
Solomon Islands ◽  
Taxonomic Status ◽  
Genetic Distances ◽  
Allozyme Data ◽  
Separate Species ◽  
Bismarck Archipelago ◽  
A Minor ◽  
Two Populations

The taxonomic status of fruitbats belonging to the genera Nyctimene and Rousettus from the Solomon Islands was investigated using allozyme electrophoresis. Two populations from the Bismarck Archipelago (Papua New Guinea) were included as reference profiles. The allozyme data at 23 loci assigned all specimens into either Nyctimene albiventer or Nyctimene major. The N. albiventer specimens comprised two subspecies, N. a. papuanus from the Bismarck Archipelago and N. a. bougainville from the Solomon Islands. No support was evident for bougainville being a separate species, and indeed the data suggest that N. a. bougainville encompasses the previously described species N. malaita and subspecies N. a. minor. Genetic distances between populations of R. amplexicaudatus from the Bismarck Archipelago and the Solomon Islands were generally low, supporting recent morphological assessments that the subspecies hedigeri, from the majority of the Solomon Islands, should be considered synonymous with subspecies brachyotis. An individual from Choiseul (Solomon Islands) with a distinctive allozyme profile is the only evidence of taxonomic complexity in R. amplexicaudatus.

scholarly journals The Aquatic Environment as a Reservoir ofVibrio choleraeO1 in Hydrographic Basins of the State of Pernambuco, Brazil

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Carina Lucena Mendes-Marques ◽  
Vladimir da Mota Silveira Filho ◽  
Ana Paula Rocha da Costa ◽  
Mariana de Lira Nunes ◽  
Sandoval Vieira da Silva Filho ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Virulence Genes ◽  
Intergenic Spacer ◽  
Pulsed Field Gel Electrophoresis ◽  
N Gene ◽  
Intergenic Spacer Region ◽  
Quiescent Period ◽  
Pathogenic Genes ◽  
Culture Negative

After the worldwide cholera epidemic in 1993, permanent environmental monitoring of hydrographic basins was established in Pernambuco, Brazil, where cholera is endemic. After a quiescent period, 4rfbN (serogroup O1) positive water samples that were culture negative were detected by multiplex single-tube nested PCR (MSTNPCR); 2 of these were alsoctxA (cholera toxin) positive. From May to June 2012, 30V. choleraeO1 isolates were obtained by culturing samples. These isolates were analyzed for the presence of virulence genes by PCR, intergenic spacer region 16S-23S PCR (ISR-PCR), and pulsed field gel electrophoresis (PFGE). The isolates were positive for therfbN gene and negative for the assessed pathogenic genes and were classified into 2 groups by ISR and the same profile by PFGE. Close genetic similarity was observed between them (2012) and environmental strains from 2004 to 2005, indicating the permanence of endemicV. choleraeO1 in the region.

Morphological and molecular data to determine the origin and taxonomic status of Prosopis chilensis var. riojana (Fabaceae, Mimosoideae)

2003 ◽  
Vol 81 (9) ◽  
pp. 905-917 ◽  
Author(s):  
Soledad Vázquez-Garcidueñas ◽  
Ramón A Palacios ◽  
Jenny Segovia-Quiroz ◽  
Juan T Frías-Hernández ◽  
Victor Olalde-Portugal ◽  
...  
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Genetic Distances ◽  
Group Method ◽  
Sympatric Populations ◽  
Arithmetic Means ◽  
Genetic Characteristics ◽  
Genetic Traits ◽  
Prosopis Chilensis

This study analyzes the morphological and genetic characteristics of three sympatric populations of Prosopis from Argentina. Although morphological and geographical data suggest that Prosopis chilensis var. riojana is an interspecific hybrid of Prosopis chilensis var. chilensis and Prosopis flexuosa var. flexuosa, no correlation was found between morphological traits and genetic distances generated by random amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR). Genetic similarity is greater among P. chilensis var. chilensis and P. flexuosa var. flexuosa than between either of these two taxa with P. chilensis var. riojana. Also, P. chilensis var. riojana has unique genetic markers that are absent from its putative parents. Additionally, dendrograms generated by unweighted pair group method with arithmetic means (UPGMA) and Neighbor-Joining clustering criteria group P. chilensis var. chilensis and P. flexuosa var. flexuosa apart from P. chilensis var. riojana. Possibilities for the lack of congruence between morphology and RAPD markers are discussed. The results obtained are insufficient to conclusively establish the origin of P. chilensis var. riojana; however, the morphological and genetic traits observed suggest this taxon is well differentiated from P. chilensis var. chilensis and P. flexuosa var. flexuosa.Key words: genetic distances, hybridization, morphology, Prosopis, RAPD, rare taxon.

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