COMPARISON OF INTRA-ARTICULAR APPLICATION OF POLYDEOXYRIBONUCLEIC ACID AND HYALURONIC ACID IN EXPERIMENTALLY INDUCED OSTEOARTHRITIS IN RATS

2020 ◽  
Vol 23 (03) ◽  
pp. 2050009
Author(s):  
Nazım Karahan ◽  
Ilyas Arslan ◽  
Ahmet Oztermeli ◽  
Mehmet Mufit Orak ◽  
Ahmet Midi ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Acid Group ◽  
Saline Group ◽  
Knee Joints ◽  
Osteoarthritis Of The Knee ◽  
Significant Difference ◽  
The Right ◽  
Experimentally Induced

Purpose: This study was conducted to histopathologically compare the results of the administration of intra-articular PDRN and HA injections in experimentally induced OA in rats. Methods: Osteoarthritis of the knee joints was induced in a total of 30 rats. Rats were randomly divided into three groups; polydeoxyribonucleic (PDRN) acid group, hyaluronic acid (HA) group, and saline group. The PDRN group was injected with 12 mg/0.05 ccPDRN acid. The HA group was injected with 12 mg/0.05 ccHA, whereas the saline group was injected with 50 [Formula: see text]l (0.05 cc) of 0.9% sodium chloride solution. All rats were sacrificed on postinjection day 29, and the right knee joints were prepared and evaluated histologically using the Mankin scoring system. Results: Total Mankin scores showed a significant difference among all groups ([Formula: see text] = 0.001). According to bilateral comparisons performed to identify the group that showed a difference, the total Mankin scores of the PDRN acid and HA groups were found to be significantly lower than those of the saline group. In PDRN acid group, tidemark continuity wasobserved in all specimen. Conclusion: Intra-articular injections of PDRN acid resulted in greater chondroprotective effects and less degeneration than those of HA and saline in experimentally induced OA of the knee joints in rats.

scholarly journals Comparison of histological effects of polydeoxyribonucleic acid and hyaluronic acid in experimentally induced osteoarthritis of the knee joints of rats

2017 ◽  
Vol 5 (2_suppl2) ◽  
pp. 2325967117S0008
Author(s):  
Nazım Karahan ◽  
İlyas Arslan ◽  
Müfit Orak ◽  
Ahmet Midi ◽  
İstemi Yücel
Keyword(s):  
Hyaluronic Acid ◽  
Saline Solution ◽  
Acid Group ◽  
Saline Group ◽  
Knee Joints ◽  
Osteoarthritis Of The Knee ◽  
The Right ◽  
Chondroprotective Effect ◽  
Acl Transection ◽  
Experimentally Induced

Aim: The histological effects of intra-articular polydeoxyribonucleic acid and hyaluronic acid in experimentally induced osteoarthritis of the knee joints of rats were investigated. Methods: Thirty rats were divided into three groups, i.e. polydeoxyribonucleic acid group, hyaluronic acid group and saline group. Osteoarthritis of the knee joints of the rats were induced by acl- transection. The polydeoxyribonucleic group was injected with 100 µg (0.05 cc) polydeoxyribonucleic acid. The hyaluronic acid group was injected with 100 µg (0.05 cc) hyaluronic acid, and the saline group was injected with 50 µl (0.05 cc) of 0.9% sodium chloride solution. All of the rats were sacrificed on day 29 and the right knee joints were prepared, and evaluated histologically by Mankin classification. Findings: The differences in total Mankin scores between the three groups were statistically significant (P < 0.01). The differences in total Mankin scores between the polydeoxyribonucleic acid group and the hyaluronic acid group were statistically significant (P < 0.01). The differences in total Mankin scores between hyaluronic acid group and saline group were statistically significant (P < 0.01). Tidemark continuity in all the specimens of the polydeoxyribonucleic acid group was noteworthy. Conclusion: The present study shows that more chondroprotective effect and less degeneration was observed with intra-articularly delivered polydeoxyribonucleic acid compared to hyaluronic acid and saline solution in the experimentally induced osteoarthritis of the knee joints of rats.

scholarly journals Effects of Granisetron on Spinal Anesthesia-induced Hypotension

2021 ◽  
Author(s):  
Cem Koray Çataroğlu ◽  
Alp Alptekin ◽  
Aysel Gezer ◽  
Murat Sayın ◽  
Aslı Dönmez
Keyword(s):  
Heart Rate ◽  
Sodium Chloride ◽  
Spinal Anesthesia ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Elective Surgery ◽  
Demographic Data ◽  
Hyperbaric Bupivacaine ◽  
Significant Difference ◽  
Isotonic Sodium Chloride

Objective: It was aimed to evaluate the effect of intravenous (IV) granisetron used for nausea and vomiting prophylaxis on hypotension and bradycardia caused by spinal anesthesia. Methods: 120 ASA 1-2 patients undergoing elective surgery under spinal anesthesia were randomly divided into Group G (Ganisetron; n=60) and Group P (Placebo; n=60) groups. Five minutes before spinal anesthesia, Group G received 1 mg intravenous granisetron diluted in 10 mL of isotonic sodium chloride solution and Group P received 10 mL of isotonic sodium chloride solution. Spinal anesthesia with hyperbaric bupivacaine 0.5%, 15 mg at the level of L4-5 was applied for both groups. Hemodynamic data, sensory and motor block parameters were recorded before and after spinal anaesthesia every 5 minutes during 20 minutes of surgery. Results: There was no difference in the demographic data of both groups. Although hemodynamic data showed a decrease in both groups according to initial values, blood pressure measurements in group G were significantly higher than the first measure values. There was no significant difference in heart rate values between the groups. Conclusion: Intravenous granisetron reduces hypotension after spinal anesthesia, but it has no significant effect on heart rate.

scholarly journals AB0589 STUDY OF THE EFFECTIVENESS OF PELOID THERAPY IN THE TREATMENT OF OSTEOARTHRITIS OF THE KNEE JOINTS IN THE CONDITIONS OF THE SANATORIUM “ CHUVASHIYAKURORT»

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1331.2-1332
Author(s):  
N. Zhuravleva ◽  
L. Karzakova ◽  
S. Kudryashov ◽  
A. Arkhipova
Keyword(s):  
Morning Stiffness ◽  
Publishing House ◽  
Medical Rehabilitation ◽  
Knee Joints ◽  
Osteoarthritis Of The Knee ◽  
Basic Therapy ◽  
Number Of Patients ◽  
Anti Inflammatory ◽  
Peloid Therapy ◽  
The Right

Background:Peloids are successfully used in the treatment of various diseases. The sapropel mud-silt deposits of freshwater reservoirs are the most widely used for joint diseases. The sapropel mud from the freshwater lake «Kogoyar» (Russia, Cheboksary) has unique properties. The right to extract and use sapropel belongs to the sanatorium “Chuvashiyakurort” (Russia) [1]. The organic biostimulants of the sapropel mud (bitumen, fatty and humic acids, amino acids, hormones, lipids) have a pronounced anti-inflammatory effect on cartilage. High humidity (87%) provides sapropel high thermal qualities.Objectives:The aim of the research was to study the effectiveness of peloid therapy in patients with osteoarthritis of the knee joints at the II X-ray stage using the sapropel mud of Lake «Kogoyar».Methods:The object of the study was 150 patients of the sanatorium “Chuvashiyakurort” aged from 55 to 62 years (89 women and 61 men). The patients were randomly divided into 2 groups. The first group (75 people) received basic therapy - chondroprotectors (Teraflex) and nonsteroidal anti-inflammatory drugs (NSAIDs) on demand. The second group (75 people) received basic therapy with the addition of peloid application. The peloid was used in the natural, minimally modified, undiluted form. The peloid therapy protocol: the peloid was heated to 50 °C before use and applied to the affected joint with a layer of 4-5 cm thick and wrapped with a warm cloth on top. The procedure took 30 minutes. Five three-day cycles of applications with one-day breaks were performed. At the end of the procedure, a patient was unraveled, the mud was removed from him and he took a warm shower. Then he had a rest for 30 to 40 minutes in the recreation room. Before the start of treatment and after its completion, a survey of patients was conducted with the fixation of pain on the VAS scale, the duration of morning stiffness, fatigue and decreased performance, general discomfort, dependence on taking NSAIDs.Results:The analysis of the survey results revealed a decrease in VAS pain and morning stiffness in both groups. However, patients in the second group had a more pronounced reduction in the average VAS pain score (26% lower compared to the first group) and the duration of morning stiffness (20% lower compared to the first group). In addition, the number of patients who indicated a decrease in dependence on taking NSAIDs was 28% higher than in the group that received only standard therapy.Conclusion:Peloid therapy enabled to reduce the pain on the back, reduce the frequency of the need for NSAIDs, improve the condition of patients, which is the reflection of the positive clinical dynamics in patients with osteoarthritis of the knee joints, and contributed to the effectiveness of treatment.References:[1]Sakharova A.S. Modern methods of medical rehabilitation in the practice of doctors of various specialties. Cheboksary: Publishing House of Chuvash State University, 2019: 110-115.Disclosure of Interests:None declared.

scholarly journals Functional and Radiological Comparison of Umbilical Cord Mesenchymal Stem Cells, Somatotropin, and Hyaluronic Acid Injection for Cartilage Repair in Early Osteoarthritis of the Knee: A Randomized Controlled Trial

2020 ◽  
Vol 8 (5_suppl5) ◽  
pp. 2325967120S0004
Author(s):  
J Fiolin ◽  
IH Dilogo ◽  
AMT Lubis ◽  
JA Pawitan ◽  
IK Liem ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Knee Osteoarthritis ◽  
Umbilical Cord ◽  
Controlled Trial ◽  
Osteoarthritis Of The Knee ◽  
Significant Difference ◽  
Group A ◽  
Ikdc Score

The umbilical cord-mesenchymal stem cells (UC-MSC) has been shown to improve the viability of degenerated chondrocytes in knee osteoarthritis (KOA) in cellular level. Additional somatotropin injection also shown clinical improvement in patients. The present trial is conducted to assess the efficacy of umbilical cord mesenchymal stem cells (UC-MSC) in comparison to somatotropin and hyaluronic acid (HA) injection to treat and slow the progression of knee osteoarthritis. Methods: This study was conducted from January 2016 to April 2018 in Cipto Mangunkusumo General Hospital. A total of 28 knees from 15 patients with early KOA Kellgren Lawrence I-II were randomized into three groups. Group A was treated with 1x106 units of intra-articular UC-MSCs + 2 ml HA followed with 2 consecutive weeks of HA injection; group B was treated with the same dose of HA with additional 8 IU of Somatotropin; group C was treated as control. The International Knee Documentation Committee (IKDC) score, The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), visual analogue score (VAS), were assessed on the 1st and 3rd month, then every 3rd month until 12 months. Cartilage evaluation using MRI cartigram were performed at pre-implantation, and after the 6th and 12th month after implantation Results: General improvement were observed in all groups after 6 and 12 months. The IKDC score improved significantly among groups after 12 months (69.43 [48.3-89.66], 79.31 [51.72-90.08], and 75.9 [67.82-97.7] in group A, B, and C, respectively, p =0.005). We did not find a significant difference between groups in VAS and WOMAC results. However, we observed a medial T2 improvement in group A (39.55 [32.65-67.85] initially and 45.64 [38.12-70.65] 12 months after implantation). Conclusion: Injection of UC-MSC could improve knee function and cartilage profile in early KOA. However, further multicenter studies with larger samples are required to investigate the efficacy of such treatment for treating knee osteoarthritis.

Use of Hyaluronic Acid Gel Filler versus Sterile Water in the Treatment of Intractable Plantar Keratomas

2015 ◽  
Vol 105 (1) ◽  
pp. 22-26
Author(s):  
Magali Brousseau-Foley ◽  
Vincent Cantin
Keyword(s):  
Hyaluronic Acid ◽  
Treatment Options ◽  
Water Injection ◽  
Acid Group ◽  
Sterile Water ◽  
Significant Difference ◽  
Gel Injection ◽  
Acid Gel ◽  
A Minor ◽  
Hyaluronic Acid Gel

Background Intractable plantar keratoma is a common concern in the podiatric medical office. Different treatment options are available, ranging from trimming and padding to surgery. We sought to investigate the use of hyaluronic acid gel injections as a possible minimally invasive alternative in the treatment of intractable plantar keratomas. Methods Seventeen patients with intractable plantar keratomas were randomly assigned to receive a hyaluronic acid gel injection or a sterile water injection at the site of a previously trimmed plantar lesion. Results There was no significant difference between the two groups in the evaluation of pain and function at 12 weeks, but both groups showed a clinically relevant improvement. No significant change was observed in plantar tissue thickness in both groups. A minor adverse reaction was seen in the hyaluronic acid group. Conclusions The use of a hyaluronic acid gel injection at the site of a trimmed intractable plantar keratoma did not seem more effective than the use of a sterile water injection. Sterile water injections seemed safe and efficient in reducing pain associated with plantar keratomas. Further investigations should concentrate on whether these results are reproducible in a larger sample and on the most effective sequence of treatment.

scholarly journals Treatment Efficacy with a Novel Hyaluronic Acid-Based Hydrogel for Osteoarthritis of the Knee

2021 ◽  
Vol 11 (4) ◽  
pp. 303
Author(s):  
Octav Marius Russu ◽  
Tudor Sorin Pop ◽  
Andrei Marian Feier ◽  
Cristian Trâmbițaș ◽  
Zsuzsanna Incze-Bartha ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Subscale Score ◽  
Osteoarthritis Of The Knee ◽  
Knee Oa ◽  
Womac Index ◽  
Significant Decrement ◽  
Symptomatic Knee ◽  
Pain Subscale ◽  
Significant Difference ◽  
Prolonged Duration

Background: Prior trials investigating the treatment of symptomatic osteoarthritis (OA) with hyaluronic-acid-derived products injections have provided optimistic results. The study was directed to assess the effectiveness of an innovative hyaluronic-acid-based hydrogel (Hymovis®) in the treatment of symptomatic knee OA. Methods: A prospective, single-center, clinical trial was performed. Thirty-five patients with degenerative knee OA were included. Inclusion criteria were: age between 45–80, radiographic Kellgren grade II or III osteoarthritis, minimum 35 mm score on the Visual Analogue Scale (VAS), pain for at least 6 months and agreement to participate in the study. Patients received two injections at a one-week interval. The evaluator assessed the patients using the Western Ontario and McMaster University Osteoarthritis Index (WOMAC) and VAS. Evaluation was performed before, at 2 and 6 months after the injections. Results: A significant improvement on the WOMAC Index pain subscale was observed at 6 months after the injection. At two months, pain subscale score decreased from 10.34 to 9.34. At six months, a significant decrement in pain parameters compared to baseline was observed (from 10.34 to 7.72; p = 0.0004). Median points on VAS significantly ameliorated after 6 months (from 74.2 to 57.3 cm; p < 0.0001). Regarding physical function, a statistically significant difference compared to baseline was observed at the end of the study (from 29.74 to 25.18; p = 0.0025). WOMAC Index stiffness component did not differ from baseline at any time during follow-up. Conclusions: Pain relief installed with a delayed on-set but had a prolonged duration. The novel hyaluronic acid-based hydrogel (Hymovis®) had effective results, particularly after six months post-injections and offers a therapeutic advancement in the treatment of moderate to severe osteoarthritis.

scholarly journals The Effect of Phospholipids (Surfactant) on Adhesion and Biomechanical Properties of Tendon: A Rat Achilles Tendon Repair Model

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
T. Kursat Dabak ◽  
Omer Sertkaya ◽  
Nuray Acar ◽  
B. Ozgur Donmez ◽  
Ismail Ustunel
Keyword(s):  
Hyaluronic Acid ◽  
Multiple Dose ◽  
Tendon Repair ◽  
Biomechanical Properties ◽  
Acid Group ◽  
Control Group ◽  
Injection Group ◽  
Microscopic Evaluation ◽  
Significant Difference ◽  
And Control

Adhesion of the tendon is a major challenge for the orthopedic surgeon during tendon repair. Manipulation of biological environment is one of the concepts to prevent adhesion. Lots of biochemicals have been studied for this purpose. We aimed to determine the effect of phospholipids on adhesion and biomechanical properties of tendon in an animal tendon repair model. Seventy-two Wistar rats were divided into 4 groups. Achilles tendons of rats were cut and repaired. Phospholipids were applied at two different dosages. Tendon adhesion was determined histopathologically and biomechanical test was performed. At macroscopic evaluation of adhesion, there are statistically significant differences between multiple-dose phospholipid injection group and Control group and also hyaluronic acid group and Control group (p<0.008). At microscopic evaluation of adhesion, there was no statistically significant difference (p>0.008). Ultimate strength was highest at hyaluronic acid injection group and lowest at multiple-dose phospholipid injection group. Single-dose phospholipids (surfactant) application may have a beneficial effect on the tendon adhesion. Although multiple applications of phospholipids seem the most effective regime to reduce the tendon adhesion among groups, it deteriorated the biomechanical properties of tendon.

scholarly journals Comparative Evaluation of The Efficacy of Topical Hyaluronic Acid ( 0.2%) and Topical Triamcinolone Acetonide( 0.1%) in The Treatment of Recurrent Aphthous Stomatitis

2020 ◽  
Vol 19 (2) ◽  
pp. 107-117
Author(s):  
Bero Nzhad Mustafa ◽  
◽  
Ali Fakhree Alzubaidee
Keyword(s):  
Hyaluronic Acid ◽  
Triamcinolone Acetonide ◽  
Surface Area ◽  
Comparative Evaluation ◽  
Acid Group ◽  
Recurrent Aphthous Stomatitis ◽  
Aphthous Stomatitis ◽  
Aphthous Ulcer ◽  
Aphthous Ulcers ◽  
Significant Difference

Background: Recurrent aphthous ulcers are the most common oral lesion. They are classified into minor, major, and herpetiform ulcers. More than 85% of recurrent aphthous stomatitis presents as a minor ulcer. Hyaluronic acid is a biomaterial and it is a major carbohydrate component that can be found in many tissues and recently introduced as an alternative approach to enhance wound healing. Triamcinolone acetonide is a synthetic corticosteroid and it has two forms for topical use cream (0.1%) and ointment (0.1%). Objective: To make a comparison between the effectiveness of hyaluronic acid (0.2%) and triamcinolone acetonide (0.1) in the management of recurrent aphthous ulcer. Patients and Methods: We recruited eighty patients who had a history of recurrent aphthous stomatitis and when presented with current oral ulcer and randomly divided into two groups, one group received hyaluronic acid, and the other group received triamcinolone acetonide. The instruction was given to all patients to apply the agent to the aphthous ulcer 4 times per day for 6 days (day 0 to day 6). The severity of pain was assessed by using VAS and the change of the ulcer surface area measured. Results: Eighty patients with aphthous ulcers have participated in the study, 43 were treated with hyaluronic acid, 37 were treated with triamcinolone acetonide. There was a significant difference between the two groups regarding ulcer surface and after three and six days, the diameter of the ulcer in the hyaluronic acid group was significantly reduced in comparison with those of the triamcinolone acetonide group (p < 0.001). Regarding VAS for pain, there was a significant difference after three and six days, hyaluronic acid group had significantly less VAS than those of the triamcinolone acetonide group (p = 0.004 and p < 0.001 respectively). Conclusion: Hyaluronic acid is more effective than triamcinolone acetonide when used in the treatment of recurrent oral ulcers in reducing pain and surface area of the ulcer. Keywords: Hyaluronic acid (HA), Triamcinolone acetonide (TA), Recurrent aphthous stomatitis (RAS), comparative evaluation

scholarly journals Clinical efficacy of dietary supplement Cartilox in osteoarthritis

2021 ◽  
Vol 59 (4) ◽  
pp. 450-454
Author(s):  
I. S. Dydykina ◽  
P. S. Kovalenko ◽  
L. V. Menshikova
Keyword(s):  
Hyaluronic Acid ◽  
Dietary Supplement ◽  
Clinical Efficacy ◽  
Type Ii Collagen ◽  
Knee Joints ◽  
Osteoarthritis Of The Knee ◽  
Type Ii ◽  
Boswellia Serrata ◽  
Collagen Peptide ◽  
Active Substances

The paper discusses the results and substantiates the effectiveness of pharmacotherapy for osteoarthritis of the knee joints using a dietary supplement Cartilox, which includes five active substances (type II collagen peptide, Boswellia serrata extract, curcuminoids, piperine and hyaluronic acid).

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