Efficient Detection of Gastric Cancer Marker miR-185 Using Graphene Oxide and Nuclease DSN

NANO ◽  
2022 ◽  
Author(s):  
Sen Li ◽  
Shaoxian Yin ◽  
Qisheng Fu ◽  
Yuanyuan Chen ◽  
Yanfei Cai ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Graphene Oxide ◽  
Clinical Diagnosis ◽  
Signal Amplification ◽  
Prognostic Biomarker ◽  
Quantitative Detection ◽  
Fluorescent Signal ◽  
Efficient Detection ◽  
Highly Sensitive ◽  
Sensitive Method

Since miR-185 has been identified as a prognostic biomarker to forecast the course of survival and relapse in gastric cancer (GC), quantitative detection of miR-185 features in developing personalized strategies for GC treatment. In this study, a highly sensitive method for miR-185 detection was rationally designed with the characteristic of fluorescent signal amplification and it was based on constructing graphene oxide sensor and utilizing duplex specific nuclease (DSN). In detail, the cleavage of many DNA signal probes was successfully triggered by the miR-185 target which contributed to the target-recycling mechanism. The protocol exhibited a prominent ability to analyze miR-185 in solution, and it can detect miR-185 at different concentrations as low as 476 pM with a linear range of 0–50 nM. Moreover, this method has gained its prominence in distinguishing the target miRNA from various sequences with one to three base mismatches or other miRNAs. Taken together, it presented the prominent potential to be a candidate tool in the field of clinical diagnosis considering its precise and efficient ability to detect miR-185.

scholarly journals Utilizing DNase I and graphene oxide modified magnetic nanoparticles for sensing PD-L1 in human plasma

Sensor Review ◽  
2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Xudong He ◽  
GuangYi Yang ◽  
E. Yang ◽  
Moli Zhang ◽  
Dan Luo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Magnetic Nanoparticles ◽  
Design Methodology ◽  
Dnase I ◽  
Content Type ◽  
Diagnosis And Prognosis ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Modified Magnetic Nanoparticles ◽  
Sensitive Method

Purpose Based on DNase I and reduced graphene oxide (rGO)-magnetic silicon microspheres (MNPS), a highly sensitive and selective fluorescent probe for the detection of PD-L1 was developed. Design/methodology/approach Here °C we present a feasibility of biosensor to detection of PD-L1 in lung tumors plasma. In the absence of PD-L1°C the PD-L1 aptamer is absorbed on the surface of graphene oxide modified magnetic nanoparticles °8rGO-MNPS°9 and leading to effective fluorescence quenching. Upon adding PD-L1°C the aptamer sequences could be specifically recognized by PD-L1 and the aptamer/PD-L1 complex is formed°C resulting in the recovery of quenched fluorescence. Findings This sensor can detect PD-L1 with a linear range from 100 pg mL−1 to 100 ng mL−1, and a detection limit of 10 pg•m−1 was achieved. Originality/value This method provides an easy and sensitive method for the detection of PD-L1 and will be beneficial to the early diagnosis and prognosis of tumors.

scholarly journals Sensitive and enzyme-free fluorescence polarization detection for miRNA-21 based on decahedral sliver nanoparticles and strand displacement reaction

RSC Advances ◽  
2020 ◽  
Vol 10 (29) ◽  
pp. 17037-17044
Author(s):  
Qingyue Zhu ◽  
Hui Li ◽  
Danke Xu
Keyword(s):  
Fluorescence Polarization ◽  
Signal Amplification ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Highly Sensitive ◽  
Polarization Detection ◽  
Sliver Nanoparticles ◽  
Sensitive Method ◽  
Strand Displacement Reaction

A highly sensitive method for miRNA-21 detection has been developed, which relied on the principle of strand displacement reaction to achieve asymmetric signal amplification and combined with the enhanced effect of Ag10NPs.

Highly sensitive DNA-based fluorometric mercury(II) bioassay based on graphene oxide and exonuclease III-assisted signal amplification

2015 ◽  
Vol 182 (7-8) ◽  
pp. 1535-1541 ◽  
Author(s):  
Yulin Zhang ◽  
Lina Tang ◽  
Fan Yang ◽  
Zhongyue Sun ◽  
Guo-Jun Zhang
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Exonuclease Iii ◽  
Highly Sensitive

Highly sensitive colorimetric immunoassay for Escherichia coli O157:H7 based on probe of pseudo enzyme and dual signal amplification

2018 ◽  
Vol 10 (35) ◽  
pp. 4301-4309 ◽  
Author(s):  
Haoran Huang ◽  
Zunyi Wu ◽  
Jianfeng Huang ◽  
Guangying Zhao ◽  
Wenchao Dou
Keyword(s):  
Signal Amplification ◽  
Quantitative Detection ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Amplification System ◽  
Colorimetric Immunoassay ◽  
Dual Signal Amplification ◽  
Signal Amplification System

A sensitive colorimetric immunoassay was established for quantitative detection of E. coli O157:H7. Au@Pt with peroxidase-like activity was combined with flaky reduced graphene oxide to form a chromogenic probe of pseudo enzyme. HRP was modified on rGO-NR–Au@Pt and a dual signal amplification system was realized.

A novel graphene oxide based fluorescent nanosensing strategy with hybridization chain reaction signal amplification for highly sensitive biothiol detection

2014 ◽  
Vol 50 (80) ◽  
pp. 11879-11882 ◽  
Author(s):  
Jia Ge ◽  
Zhi-Mei Huang ◽  
Qiang Xi ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Quenching ◽  
Signal Amplification ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Highly Sensitive

A novel fluorescent nanosensor was developed for detecting biothiols via coupling graphene based fluorescence quenching with T–Hg(ii)–T controlled hybridization chain reaction.

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