Structure and Function of Chitin-Binding Proteins

1993 ◽  
Vol 44 (1) ◽  
pp. 591-615 ◽  
Author(s):  
N V Raikhel ◽  
H I Lee ◽  
W F Broekaert
Keyword(s):  
Binding Proteins ◽  
Structure And Function ◽  
And Function

scholarly journals Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Cecilia Paolini ◽  
Marco Quarta ◽  
Laura D'Onofrio ◽  
Carlo Reggiani ◽  
Feliciano Protasi
Keyword(s):  
Skeletal Muscle ◽  
Differential Effect ◽  
Structural Damage ◽  
Binding Proteins ◽  
Structure And Function ◽  
Tetanic Stimulation ◽  
Active Tension ◽  
Slow Skeletal Muscle ◽  
Time Parameters ◽  
And Function

We compared structure and function of EDL and Soleus muscles in adult (4–6 m) mice lacking both Calsequestrin (CASQ) isoforms, the main SR Ca2+-binding proteins. Lack of CASQ induced ultrastructural alterations in ~30% of Soleus fibers, but not in EDL. Twitch time parameters were prolonged in both muscles, although tension was not reduced. However, when stimulated for 2 sec at 100 hz, Soleus was able to sustain contraction, while in EDL active tension declined by 70–80%. The results presented in this paper unmask a differential effect of CASQ1&2 ablation in fast versus slow fibers. CASQ is essential in EDL to provide large amount of Ca2+released from the SR during tetanic stimulation. In contrast, Soleus deals much better with lack of CASQ because slow fibers require lower Ca2+amounts and slower cycling to function properly. Nevertheless, Soleus suffers more severe structural damage, possibly because SR Ca2+leak is more pronounced.

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