Gas Phase Deposition and Characterization of (Si)-B-C Ceramics

2019 ◽  
Vol 25 (8) ◽  
pp. 15-21 ◽  
Author(s):  
Georges Chollon ◽  
Francis Langlais ◽  
Jerome Berjonneau
Keyword(s):  
Gas Phase ◽  
Gas Phase Deposition

Surface-Controlled Gas-Phase Deposition and Characterization of Highly Dispersed Vanadia on Silica

2003 ◽  
Vol 107 (39) ◽  
pp. 10773-10784 ◽  
Author(s):  
Jetta Keranen ◽  
Claude Guimon ◽  
Eero Iiskola ◽  
Aline Auroux ◽  
Lauri Niinisto
Keyword(s):  
Gas Phase ◽  
Highly Dispersed ◽  
Gas Phase Deposition

Synthesis and Electrochemical Characterization of Carbon Supported Platinum Grown by Template Assisted Gas Phase Deposition

2013 ◽  
Vol 50 (2) ◽  
pp. 1723-1727
Author(s):  
J. Bult ◽  
K. C. Neyerlin ◽  
S. Christensen ◽  
A. Dameron ◽  
S. S. Kocha ◽  
...  
Keyword(s):  
Gas Phase ◽  
Electrochemical Characterization ◽  
Supported Platinum ◽  
Gas Phase Deposition

Characterization of 56 airborne persistent organic pollutants (POPs) in gas-phase and particle-phase

2021 ◽  
pp. 1-13
Author(s):  
Li Tan ◽  
Ning Wang ◽  
Yingjian Dong ◽  
Siyuan Li ◽  
Xuehan Wang ◽  
...  
Keyword(s):  
Gas Phase ◽  
Organic Pollutants ◽  
Persistent Organic Pollutants ◽  
Particle Phase

scholarly journals Characterization of gas-phase thorium nitride

2019 ◽  
Vol 150 (14) ◽  
pp. 144304 ◽  
Author(s):  
Anh T. Le ◽  
Sanjay G. Nakhate ◽  
Duc-Trung Nguyen ◽  
Timothy C. Steimle ◽  
Michael C. Heaven
Keyword(s):  
Gas Phase

ChemInform Abstract: Characterization of Ionized Carbenes in the Gas Phase

ChemInform ◽  
2001 ◽  
Vol 32 (12) ◽  
pp. no-no
Author(s):  
Robert Flammang ◽  
Minh Tho Nguyen ◽  
Guy Bouchoux ◽  
Pascal Gerbaux
Keyword(s):  
Gas Phase ◽  
Abstract Characterization

scholarly journals State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates Synthesis

2021 ◽  
Vol 14 (6) ◽  
pp. 498
Author(s):  
Evolène Deslignière ◽  
Anthony Ehkirch ◽  
Bastiaan L. Duivelshof ◽  
Hanna Toftevall ◽  
Jonathan Sjögren ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Phase ◽  
Ion Mobility ◽  
State Of The Art ◽  
Native Mass Spectrometry ◽  
Site Specific ◽  
Drug Conjugates ◽  
Conjugation Process ◽  
Antibody Drug

Antibody-drug conjugates (ADCs) are biotherapeutics consisting of a tumor-targeting monoclonal antibody (mAb) linked covalently to a cytotoxic drug. Early generation ADCs were predominantly obtained through non-selective conjugation methods based on lysine and cysteine residues, resulting in heterogeneous populations with varying drug-to-antibody ratios (DAR). Site-specific conjugation is one of the current challenges in ADC development, allowing for controlled conjugation and production of homogeneous ADCs. We report here the characterization of a site-specific DAR2 ADC generated with the GlyCLICK three-step process, which involves glycan-based enzymatic remodeling and click chemistry, using state-of-the-art native mass spectrometry (nMS) methods. The conjugation process was monitored with size exclusion chromatography coupled to nMS (SEC-nMS), which offered a straightforward identification and quantification of all reaction products, providing a direct snapshot of the ADC homogeneity. Benefits of SEC-nMS were further demonstrated for forced degradation studies, for which fragments generated upon thermal stress were clearly identified, with no deconjugation of the drug linker observed for the T-GlyGLICK-DM1 ADC. Lastly, innovative ion mobility-based collision-induced unfolding (CIU) approaches were used to assess the gas-phase behavior of compounds along the conjugation process, highlighting an increased resistance of the mAb against gas-phase unfolding upon drug conjugation. Altogether, these state-of-the-art nMS methods represent innovative approaches to investigate drug loading and distribution of last generation ADCs, their evolution during the bioconjugation process and their impact on gas-phase stabilities. We envision nMS and CIU methods to improve the conformational characterization of next generation-empowered mAb-derived products such as engineered nanobodies, bispecific ADCs or immunocytokines.

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