scholarly journals HPTLC Method Development and Validation for Determination of Rutin in Flavanoidal Fraction ofHibiscus micranthusLinn.

2011 ◽  
Vol 8 (3) ◽  
pp. 1444-1450 ◽  
Author(s):  
K. Ashok Kumar ◽  
S. Ramachandra Shetty ◽  
Laxmi Narasu
Keyword(s):  
High Performance ◽  
Method Development ◽  
Methanolic Extract ◽  
Method Development And Validation ◽  
Stem Extract ◽  
Acetic Acid Water ◽  
Flavonoid Fraction ◽  
Development And Validation ◽  
Hptlc Method

A simple, precise and accurate high-performance thin-layer chromatographic method has been established for the determination of rutin in the stem extract ofHibiscus micranthusLinn. A flavonoid fraction of methanolic extract of the stem powder was used for the experimental work. Separation was performed on silica gel 60 F254HPTLC plates withn-butanol: acetic acid: water: ammonia 3:1:1:1(v/v), as mobile phase. The determination was carried out using the densitometric absorbance mode at 286 nm. Rutin response was linear over the range 50–400 ng/μL. The concentration of rutin in the flavanoidal fraction was found to be 9.93%. The HPTLC method was evaluated in terms of sensitivity, accuracy, precision and reproducibility.

scholarly journals Method Development and Validation of simultaneous estimation for Amlodipine besylate and Olmesartan medoxomil by HPTLC method

2018 ◽  
Vol 9 (1) ◽  
pp. 201 ◽  
Author(s):  
Sonia K ◽  
Manikandan K ◽  
Hamunyare Ndwabe ◽  
Peddamadi Bhavya Sree ◽  
Lakshmi KS
Keyword(s):  
High Performance ◽  
Method Development ◽  
Olmesartan Medoxomil ◽  
Simultaneous Estimation ◽  
Amlodipine Besylate ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Hptlc Method

The present work deals with method development and analytical validation of a novel, precise and accurate HPTLC methods for the simultaneous estimation of Amlodipine besylate and Olmesartan medoxomil. Literature review has shown that High performance liquid chromatography and UV-Visible Spectroscopy methods have been reported for the estimation of these drugs, but no HPTLC method has been done, thus this study had to be done. A analytical method development for the simultaneous estimation of Amlodipine besylate and Olmesartan medoxomil was developed. For simultaneous HPTLC method, the analytical separation was achieved on aluminium plates pre-coated with Silica Gel 60 F254 Acetonitrile: Water: Toluene (6:3:1) v/v/v used as the mobile phase with densitometric scanning at 270 nm. Good and acceptable linearity was obtained for the drug in the range of 5 - 15 μg/mL with r2 > 0.999. All the precision measurements made were well within the acceptable limits. The percentage recovery was found to be 99.59 & 99.61 % HPTLC method for Amlodipine besylate and Olmesartan medoxomil respectively. The parameters of validation were in accordance with the outlined ICH guidelines for method development in the estimation of drugs. Hence in conclusion the method can be applied day today lab practice for the determination of Amlodipine besylate and Olmesartan medoxomil simultaneously using HPTLC instrument.

Method Development and Validation of Propofol by Reverse Phase HPLC and its Estimation in Commercial Formulation

2021 ◽  
pp. 3139-3142
Author(s):  
Kuntal Mukherjee ◽  
S. T. Narenderan ◽  
B. Babu ◽  
Survi Mishra ◽  
S. N. Meyyanathan
Keyword(s):  
High Performance ◽  
Method Development ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Reverse Phase Hplc ◽  
Liquid Chromatographic Method ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Liquid Chromatographic

A simple, sensitive and rapid high performance liquid chromatographic method has been developed for the determination of Propofol. The main focus of the method was to determine Propofol in solution form as well as in marketed formulation. Chromatographic separation was achieved on Inertsil ODS-3V column (250mm x 4.6mm; 5µm) with a mobile phase consisting of methanol: water (85:15), with a flow rate of 1.0ml/min (UV detection at 270nm). Linearity was observed over the concentration range of 10-110µg/ml with a regression equation y=88048x + 44524 and having a regression value (R2) of 0.999. The LOD and LOQ values found to be 10ng and 100ng, respectively. No changes found in ruggedness and robustness studies. The percentage of recovery was found to be 95.25% to 101.81%. Validation studies revealed that the method was specific, accurate, precise, reliable, robust, reproducible and suitable for the quantitative analysis in its pharmaceutical formulations.

scholarly journals METHOD DEVELOPMENT AND VALIDATION OF EPROSARTAN MESYLATE AND ITS IMPURITIES USING REVERSE PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

2016 ◽  
Vol 8 (4) ◽  
pp. 49 ◽  
Author(s):  
O. S. S. Chandana ◽  
D. Sathis Kumar ◽  
R. Ravichandra Babu
Keyword(s):  
High Performance ◽  
Method Development ◽  
Hplc Method ◽  
Reverse Phase ◽  
Related Substances ◽  
Rp Hplc ◽  
Method Development And Validation ◽  
The Mean ◽  
Development And Validation

Objective: Our main objective is to develop an accurate and precise RP-HPLC method for the determination of Eprosartan Mesylate and its impurities. Methods: A Develosil ODS UG-5; (150 × 4.6) mm; 5 µm column was used for the Separation of drugs by a mobile phase consisting of Buffer and Acetonitrile mixture in the gradient proportion. The flow rate maintained was 0.8 ml/min and the wavelength used for detection was 235 nm.Results: The linearity was observed in the range of 0.025-50µg/ml of spiked impurities in Eprosartan Mesylate, impurity 1 and impurity 2 with a correlation coefficient of 0.99927, 0.99910 and 0.99934 respectively. The mean percentage recoveries for LOQ, 50%, 80%, 100%, 150% and 200% accuracy were found to be 101.5±1.51, 107.0±1.7, 104.6±0.4, 102.8±0.36, 101.7±0.26 and 101.3±0.15 respectively for impurities in Eprosartan Mesylate, impurity 1 and impurity 2. Linearity, accuracy, precision and robustness parameters for the suggested method were estimated for validation.Conclusion: The developed method is uncomplicated, accurate, sensitive and precise for the determination of related substances in the Eprosartan Mesylate. The satisfying % recoveries and low % RSD Values confirmed the suitability of the developed method for the usual analysis of Eprosartan mesylate in pharmaceuticals.

scholarly journals HPLC-MS method development and validation for simultaneous quantitation of GZK-111 and CPG compounds in rat plasma

2020 ◽  
pp. 28-36
Author(s):  
A. L. Podolko ◽  
P. O. Bochkov ◽  
G. B. Kolyvanov ◽  
A. A. Litvin ◽  
O. G. Gribakina ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Lower Limit ◽  
High Performance ◽  
Method Development ◽  
Limit Of Detection ◽  
Rat Plasma ◽  
Rat Blood ◽  
Method Development And Validation ◽  
Development And Validation

The technique of quantitative determination of GZK-111 and CPG compounds in rat blood plasma has been developed. The analysis was carried out using the combined method of high-performance liquid chromatography with mass spectrometric detection. The method was linear in the concentration range of 25-1000 ng/ml for both compounds. Percentage of GZK-111 extraction from rat blood plasma was 57.8 %. The lower limit of detection for GZK- 111 compound was 25 ng/ml. Percentage of extraction of CPG compound was 42.5 %. The lower limit of detection of CPG compound was also 25 ng/ml.

HPTLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS DETERMINATION OF RESERPINE AND GALLIC ACID IN AN AYURVEDIC FORMULATION

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (09) ◽  
pp. 55-60
Author(s):  
A Adhyapak ◽  
A. P. Jadhav ◽  
Keyword(s):  
Gallic Acid ◽  
Method Development ◽  
Simultaneous Estimation ◽  
Quality And Safety ◽  
Tlc Plates ◽  
Method Development And Validation ◽  
Rf Values ◽  
Development And Validation ◽  
Hptlc Method

A simple, precise, accurate, robust HPTLC method was developed and validated for simultaneous estimation of reserpine and gallic acid in an Ayurvedic formulation. The two markers were resolved using TLC plates pre-coated with silica gel 60F254 using the mobile phase Toluene: Acetone: Glacial acetic acid in the ratio 4:1:1 v/v with a saturation time of 10 minutes. The Rf values of gallic acid and reserpine were found to be 0.31 ± 0.2 and 0.54 ± 0.2, respectively at 268 nm. Linear response was observed in the concentration range of 20–80 ppm for reserpine and 150–210 ppm for gallic acid, with correlation coefficient (r2 ) of 0.995 and 0.997 for reserpine and gallic acid respectively. The developed method was applied for the quantitation of markers in a marketed formulation, Cardostab tablet. This method can also be used to evaluate other formulations containing reserpine and gallic acid as the constituents, thus conforming to the need of ensuring quality and safety of polyherbal formulations.

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