scholarly journals Leishmaniasp. Amastigotes Identification in Canine Transmissible Venereal Tumor

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Vinicius Soares Carreira ◽  
Heitor Flávio Ferrari ◽  
Ingeborg Maria Langohr ◽  
Charles Mackenzie ◽  
Luiz Carlos Montezzo ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Tumor Tissue ◽  
Canine Visceral Leishmaniasis ◽  
Leishmania Chagasi ◽  
Neoplastic Tissue ◽  
Mixed Breed ◽  
Route Of Transmission ◽  
Vector Borne ◽  
Polymerase Chain ◽  
Male Genital

Leishmaniasis is a vector-borne disease withLeishmania chagasibeing the etiological agent of canine visceral leishmaniasis in South America. Canine venereal tumor is a transplantable round cell tumor of histiocytic origin which is mostly observed in sexually active male and female intact dogs. It has been shown thatLeishmaniaamastigotes have higher tropism for the canine male genital tract tissues and venereal leishmaniasis transmission has been documented in dogs but, to date, a canine venereal tumor-dependent transmission route has not been fully demonstrated. In this report, a 10-year-old, mixed breed, intact female dog presented a vaginal venereal transmissible tumor but no other clinical abnormalities otherwise. Unexpectedly, tumor tissue imprint smears examination revealedLeishmaniasp. amastigotes within infiltrating macrophages. In addition to the cytological direct identification, the protozoan was confirmed within the neoplastic tissue by means of immunohistochemistry and polymerase chain reaction. This report illustrates an asymptomaticLeishmaniasp. infection that may have started on or from the canine venereal tumor tissue, the latter option further supporting previous evidence of such an alternative vector-independent route of transmission for canine visceral leishmaniasis in areas where these diseases coexist.

scholarly journals GEOGRAPHICAL EXPANSION OF CANINE VISCERAL LEISHMANIASIS IN RIO DE JANEIRO STATE, BRAZIL

2015 ◽  
Vol 57 (5) ◽  
pp. 435-438 ◽  
Author(s):  
Denise Amaro da SILVA ◽  
Maria de Fátima MADEIRA ◽  
Fabiano Borges FIGUEIREDO
Keyword(s):  
Visceral Leishmaniasis ◽  
Rio De Janeiro ◽  
Etiologic Agent ◽  
Domestic Dog ◽  
Canine Visceral Leishmaniasis ◽  
Public Agencies ◽  
Leishmania Chagasi ◽  
Geographical Expansion ◽  
Vector Borne ◽  
Janeiro State

SUMMARY Visceral Leishmaniasis (VL) is a vector-borne disease that affects humans, and domestic and wild animals. It is caused by the protozoan Leishmania (Leishmania) infantum (syn = Leishmania chagasi). The domestic dog (Canis familiaris) is considered the main reservoir of the etiologic agent of VL in domestic and peridomestic environments. In the past three years, although control actions involving domestic dogs are routinely performed in endemic areas of the Rio de Janeiro State, new cases of canine visceral leishmaniasis (CVL) have been reported in several municipalities. The objective of this short communication was to describe the geographical expansion of CVL in the Rio de Janeiro State, Brazil, through its reports in the scientific literature and studies performed by our group. From 2010 to 2013, autochthonous and allochthonous cases of CVL were reported in the municipalities of Mangaratiba, Marica, Niteroi, Barra Mansa, Cachoeiras de Macacu, Volta Redonda, Resende and Rio de Janeiro. These reports demonstrate that CVL is in intense geographical expansion around the state; therefore, a joint effort by public agencies, veterinarians and researchers is needed in order to minimize and/or even prevent the dispersion of this disease.

scholarly journals First description of autochthonous canine visceral leishmaniasis in the metropolitan region of Vitória, State of Espírito Santo, Brazil

2012 ◽  
Vol 45 (6) ◽  
pp. 754-756 ◽  
Author(s):  
Marco André Loureiro Tonini ◽  
Elenice Moreira Lemos ◽  
Alexandre Barbosa Reis ◽  
Wendel Coura Vital ◽  
Edelberto Santos Dias ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Positive Culture ◽  
Human Case ◽  
Metropolitan Region ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis ◽  
Leishmania Chagasi ◽  
Molecular Tests ◽  
Leishmania Spp ◽  
Polymerase Chain

INTRODUCTION: We investigated autochthonous canine visceral leishmaniasis (CVL) in the metropolitan region of Vitória (MRV), an area in which a human case was previously reported. METHODS: Serological, parasitological, and molecular tests were performed in 201 dogs. RESULTS: Twenty-six (13%) and 12 (6%) dogs were identified as positive using in-house enzyme-linked immunosorbent assay (ELISA) and rK39 tests, respectively. Two dogs had a positive culture for Leishmania chagasi, and 4 were polymerase chain reaction (PCR)-positive for Leishmania spp. One positive dog belonged to the aforementioned patient. CONCLUSIONS: Although the responsible vector was not found, our results provide evidence of autochthonous CVL in the MRV, a non-endemic area for VL.

scholarly journals First autochthonous case of canine visceral leishmaniasis in Volta Redonda, Rio de Janeiro, Brazil

2013 ◽  
Vol 22 (3) ◽  
pp. 424-426 ◽  
Author(s):  
Monique Paiva de Campos ◽  
Denise Amaro da Silva ◽  
Maria de Fátima Madeira ◽  
Artur Augusto Mendes Velho Júnior ◽  
Fabiano Borges Figueiredo
Keyword(s):  
Visceral Leishmaniasis ◽  
Diagnostic Tests ◽  
Rio De Janeiro ◽  
Scientific Community ◽  
Clinical Manifestations ◽  
Canine Visceral Leishmaniasis ◽  
Leishmania Chagasi ◽  
Laboratory Findings ◽  
Autochthonous Case ◽  
Canine Diseases

In Brazil, American visceral leishmaniasis (AVL) is caused byLeishmania (Leishmania) chagasi and its main vector isLutzomyia longipalpis. Cases of canine visceral leishmaniasis (CVL) in non-endemic areas have been reported over the last few years throughout the country. The objective of this research note is to describe an autochthonous case of CVL that occurred in the municipality of Volta Redonda, state of Rio de Janeiro, an area where the disease is not endemic, alerting veterinarians and the scientific community to the expansion of this important zoonosis and advising veterinary practitioners on how to deal with a suspicion of CVL. Canine visceral leishmaniasis can be misdiagnosed within a broad spectrum of canine diseases based on clinical and laboratory findings. Therefore, knowledge of its clinical manifestations, specific and sensitive laboratory diagnostic tests and parasitological procedures are of the utmost importance for rapid confirmation and notification of a case, thus contributing directly to the control of a focus.

Comparison of the Polymerase Chain Reaction and Serology for the Detection of Canine Visceral Leishmaniasis

1995 ◽  
Vol 53 (3) ◽  
pp. 251-255 ◽  
Author(s):  
David A. Ashford ◽  
Wim Degrave ◽  
Conceicao Eulalio ◽  
Roberto Badaro ◽  
Ulisses Lopes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Canine Visceral Leishmaniasis ◽  
Chain Reaction ◽  
Polymerase Chain

Canine visceral leishmaniasis: diagnostic approaches based on polymerase chain reaction employing different biological samples

2013 ◽  
Vol 76 (3) ◽  
pp. 321-324 ◽  
Author(s):  
Arleana B.P.F. Almeida ◽  
Valéria R.F. Sousa ◽  
Naiani D. Gasparetto ◽  
Givago F.R. da Silva ◽  
Fabiano B. Figueiredo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Biological Samples ◽  
Canine Visceral Leishmaniasis ◽  
Chain Reaction ◽  
Diagnostic Approaches ◽  
Polymerase Chain

scholarly journals T-cell-derived cytokines, nitric oxide production by peripheral blood monocytes and seric anti-Leishmania (Leishmania) chagasi IgG subclass patterns following immunization against canine visceral leishmaniasis using Leishvaccine and Leishmune®

Vaccine ◽  
2009 ◽  
Vol 27 (7) ◽  
pp. 1008-1017 ◽  
Author(s):  
Márcio Sobreira Silva Araújo ◽  
Renata Aline de Andrade ◽  
Renato Sathler-Avelar ◽  
Andréa Teixeira-Carvalho ◽  
Mariléia Chaves Andrade ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
T Cell ◽  
Visceral Leishmaniasis ◽  
Peripheral Blood ◽  
Nitric Oxide Production ◽  
Igg Subclass ◽  
Canine Visceral Leishmaniasis ◽  
Blood Monocytes ◽  
Leishmania Chagasi ◽  
Peripheral Blood Monocytes

scholarly journals Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

2010 ◽  
Vol 52 (4) ◽  
pp. 193-196 ◽  
Author(s):  
Fabiano B. Figueiredo ◽  
Maria F. Madeira ◽  
Lílian D. Nascimento ◽  
Tuanne R. Abrantes ◽  
Eliame Mouta-confort ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Filter Paper ◽  
Rio De Janeiro ◽  
Serological Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Canine Visceral Leishmaniasis ◽  
Leishmania Chagasi ◽  
Blood Samples ◽  
One Year

The Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL). In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT), using blood samples eluted on filter paper (eluate). A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical examination, and blood samples (serum and eluate) were collected for analysis by enzyme immunoassay (ELISA) and IFAT. A skin biopsy was obtained for parasitological examination (culture). A total of 305 animals were studied and Leishmania chagasi was isolated from nine animals. Sensitivity and specificity were 100% and 96.6% for ELISA, respectively, 100% and 65.5% for IFAT (cut-off at a 1:40 dilution), 100% and 83.4% for IFAT (cut-off at a 1:80 dilution), and 22.2% and 97.0% for eluate IFAT. In conclusion, ELISA was the best tool for the diagnosis of CVL among the serological techniques tested. The present results suggest the need for a better evaluation of filter paper IFAT as the only diagnostic method for CVL in the Municipality of Rio de Janeiro.

scholarly journals Specific Serodiagnosis of Canine Visceral Leishmaniasis Using Leishmania Species Ribosomal Protein Extracts

2009 ◽  
Vol 16 (12) ◽  
pp. 1774-1780 ◽  
Author(s):  
Eduardo A. F. Coelho ◽  
Laura Ramírez ◽  
Mariana A. F. Costa ◽  
Vinicio T. S. Coelho ◽  
Vivian T. Martins ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Ribosomal Proteins ◽  
High Sensitivity ◽  
Leishmania Species ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis ◽  
Leishmania Chagasi ◽  
Serum Samples ◽  
Potential Tool

ABSTRACT In the present work, we have analyzed the antigenicity of Leishmania species ribosomal proteins (LRPs). To accomplish this, Leishmania infantum ribosomes were biochemically purified from promastigote cytosolic extracts, and their reactivities were analyzed by using the sera from dogs naturally infected with L. infantum. Since antibodies reacting against different ribosomal proteins were observed in all the serum samples obtained from dogs with symptomatic visceral leishmaniasis tested, we have analyzed the potential usefulness of the LRP extracts in the development of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of canine visceral leishmaniasis (CVL) in an area of Brazil where visceral leishmaniasis is endemic due to infection by Leishmania chagasi. A comparative ELISA with crude soluble Leishmania chagasi antigen (SLA) and L. infantum LRPs was performed. LRP- and SLA-based ELISAs gave similar sensitivities for the diagnosis of symptomatic CVL, but the LRP extract provided a very high sensitivity for the detection of oligosymptomatic and asymptomatic dogs. In addition, an LRP-based ELISA showed a higher specificity when the sera from dogs harboring other infections were included in the analysis. The LRP antigen displayed no cross-reactivity with sera from dogs that had any of the other diseases tested, notably, Chagas' disease. Our findings suggest that LRPs are a potential tool for the diagnosis of CVL and will be particularly useful for the diagnosis of asymptomatic CVL.

Assessment of a New Antigen Detection Test for the Diagnosis of Canine Visceral Leishmaniasis

2021 ◽  
Author(s):  
Claudia Abeijon ◽  
Stefano Pizzirani ◽  
Antonio Campos-Neto
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Protein Detection ◽  
Antigen Detection ◽  
Canine Visceral Leishmaniasis ◽  
Chain Reaction ◽  
Serological Tests ◽  
Polymerase Chain ◽  
Antigen Protein ◽  
Simple Exposure

Canine visceral leishmaniasis (CVL) is a serious zoonotic disease in Brazil and Southern Europe. CVL is primarily caused by Leishmania infantum and its diagnosis relies primarily on detection of parasites in bone marrow or lymph node aspirates by microscopic observation of the parasites in stained smears, parasite culture, or polymerase chain reaction (PCR). Serological tests exist but they do not distinguish active disease from simple exposure to parasite antigens. Here, we have assessed the utility of a new monoclonal antibody––based antigen (protein) detection test for the diagnosis of CVL. The test was positive in 70% of beagle dogs experimentally infected with L. infantum. In contrast, culture of the parasites from bone marrow aspirates was positive in only 40% of the infected animals. These preliminary results suggest that this antigen detection test, which we have recently described for the diagnosis of human VL, has the potential to be a useful diagnostic tool for CVL.

A recombinant cysteine proteinase from Leishmania (Leishmania) chagasi as an antigen for delayed-type hypersensitivity assays and serodiagnosis of canine visceral leishmaniasis

2009 ◽  
Vol 162 (1-2) ◽  
pp. 32-39 ◽  
Author(s):  
Paulo Henrique da Costa Pinheiro ◽  
Adriana Nunes Pinheiro ◽  
Josie Haydée Lima Ferreira ◽  
Francisco Assis Lima Costa ◽  
Simone Katz ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Cysteine Proteinase ◽  
Delayed Type Hypersensitivity ◽  
Canine Visceral Leishmaniasis ◽  
Leishmania Chagasi
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