scholarly journals Improvement in Tracing Quantum Dot-Conjugated Nanospheres forIn VivoImaging by Eliminating Food Autofluorescence

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Chul-Kyu Park ◽  
Hoonsung Cho
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Signal To Noise Ratio ◽  
Tissue Imaging ◽  
Fluorescence Probes ◽  
Infrared Range ◽  
Long Term Stability ◽  
Magnetic Nanospheres ◽  
Deep Tissue Imaging

Fluorescence imaging using fluorescent probes has demonstrated long-term stability and brightness suitable forin vivodeep-tissue imaging, but it also allows intense background fluorescence associated with food in the near-infrared (IR) range. We investigated effects of changing rodent diet on food autofluorescence, in the presence of quantum dots-conjugated magnetic nanospheres (QD-MNSs). Replacement of a regular rodent diet with a purified diet has great improvement in removing autofluorescence in the near-infrared range ideal forin vivofluorescence imaging. By feeding a purified diet for eliminating ingredients impairing desirable fluorescence signals in the near-IR range, food autofluorescence was clearly eliminated and fluorescence probes, QD-MNSs, introduced by i.v. injection were effectively traced in a mouse by a distinctive signal-to-noise ratio.

scholarly journals Imaging of red-shifted photons from bioluminescent tumours using fluorescence by unbound excitation from luminescence

2018 ◽  
Author(s):  
Fabiane Sônego ◽  
Sophie Bouccara ◽  
Thomas Pons ◽  
Nicolas Lequeux ◽  
Anne Danckaert ◽  
...  
Keyword(s):  
Cell Line ◽  
Near Infrared ◽  
Tumour Size ◽  
Visible Spectrum ◽  
Tissue Imaging ◽  
Tumour Detection ◽  
Signal Sensitivity ◽  
Bioluminescence Signal ◽  
Deep Tissue Imaging

AbstractEarly detection of tumours is today a major challenge and requires sensitive imaging methodologies coupled with new efficient probes. Bioluminescence imaging has been widely used in the field of oncology and several cancer cell lines have been genetically modified to provide bioluminescence signals. However, photons that are emitted by the majority of commonly used luciferases are usually in the blue part of the visible spectrum, where tissue absorption is still very high, making deep tissue imaging non-optimal and calling for optimised optical imaging methodologies. We have previously shown that red-shifting of bioluminescence signal by Fluorescence Unbound Excitation from Luminescence (FUEL) is a mean to increase bioluminescence signal sensitivity detection in vivo. Here, we applied FUEL to tumour detection in two different subcutaneous tumour models: the auto-luminescent human embryonic kidney (HEK293) cell line and the murine B16-F10 melanoma cell line previously transfected with the plasmid Luc2. Tumour size and bioluminescence were measured over time and tumour vascularization characterized. We then locally injected near infrared emitting Quantum Dots (NIR QDs)in the tumour site and observed a red-shifting of bioluminescence signal by (FUEL) indicating that FUEL could be used to allow deeper tumour detection.

Design of AIEgens for Near-Infrared IIb Imaging Through Structural Modulation at Molecular and Morphological Levels

2019 ◽  
Author(s):  
yuanyuan li ◽  
Zhaochong Cai ◽  
shunjie liu ◽  
Haoke Zhang ◽  
sherman Wong ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Molecular Design ◽  
Organic Dyes ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
Structural Modulation ◽  
Organic Fluorophore

<p>Fluorescence imaging in near-infrared IIb (NIR-IIb, 1500-1700 nm) spectrum holds a considerable promise for tissue imaging with deep penetration and high spatial resolution owing to the minimized autofluorescence and suppressed photon scattering. While few inorganic NIR-IIb fluorescent probes have been reported, their organic counterparts are still underdeveloped, possibly due to the lack of efficient materials with long emission wavelength. Herein, we propose a new molecular design philosophy to develop organic NIR-IIb fluorophores with high quantum yield (QY) by manipulation of the effects of twisted intramolecular charge transfer and aggregation-induced emission at the molecular and morphological levels. A pure organic fluorescent dye emitting up to 1600 nm with a QY of 14.2% in the NIR-II region (1000-1600 nm) is developed. For the first time, NIR-IIb fluorescence imaging of blood vessels and deeply-located intestinal tract of live mice based on organic dyes is achieved. The results show that organic fluorophore performs superb imaging ability in both superficial blood vessels and internal organs with high resolution and enhanced signal-to-background ratio in NIR-IIb region. We hope this groundbreakingly study will inspire further research on the evolution of pure organic NIR-IIb probes for in vivo imaging.</p>

A near-infrared I emissive dye: toward the application of saturable absorber and multiphoton fluorescence microscopy in the deep-tissue imaging window

2019 ◽  
Vol 55 (35) ◽  
pp. 5111-5114 ◽  
Author(s):  
Can Ren ◽  
Xiangquan Deng ◽  
Wenbo Hu ◽  
Junzi Li ◽  
Xiaofei Miao ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Saturable Absorber ◽  
Near Infrared ◽  
Tissue Imaging ◽  
Saturable Absorption ◽  
Deep Tissue ◽  
Boron Dipyrromethene ◽  
Deep Tissue Imaging ◽  
Photon Fluorescence

A boron-dipyrromethene (BODIPY) dye emitting in the near-infrared (NIR) I region (723 nm) exhibits strong saturable absorption at 680 nm and excellent three-photon fluorescence imaging in the NIR II (1665 nm) window.

Five-nanometer ZnSn2O4:Cr,Eu ultra-small nanoparticles as new near infrared-emitting persistent luminescent nanoprobes for cellular and deep tissue imaging at 800 nm

Nanoscale ◽  
2017 ◽  
Vol 9 (25) ◽  
pp. 8631-8638 ◽  
Author(s):  
Jin-Lei Li ◽  
Jun-Peng Shi ◽  
Cheng-Cheng Wang ◽  
Peng-Hui Li ◽  
Zhen-Feng Yu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging ◽  
Small Nanoparticles

Schematic illustration of the synthesis, functionalization and repeated in vivo simulated deep tissue imaging of ZSO NPLNPs.

Design of AIEgens for Near-Infrared IIb Imaging Through Structural Modulation at Molecular and Morphological Levels

2019 ◽  
Author(s):  
yuanyuan li ◽  
Zhaochong Cai ◽  
shunjie liu ◽  
Haoke Zhang ◽  
sherman Wong ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Molecular Design ◽  
Organic Dyes ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
Structural Modulation ◽  
Organic Fluorophore

<p>Fluorescence imaging in near-infrared IIb (NIR-IIb, 1500-1700 nm) spectrum holds a considerable promise for tissue imaging with deep penetration and high spatial resolution owing to the minimized autofluorescence and suppressed photon scattering. While few inorganic NIR-IIb fluorescent probes have been reported, their organic counterparts are still underdeveloped, possibly due to the lack of efficient materials with long emission wavelength. Herein, we propose a new molecular design philosophy to develop organic NIR-IIb fluorophores with high quantum yield (QY) by manipulation of the effects of twisted intramolecular charge transfer and aggregation-induced emission at the molecular and morphological levels. A pure organic fluorescent dye emitting up to 1600 nm with a QY of 14.2% in the NIR-II region (1000-1600 nm) is developed. For the first time, NIR-IIb fluorescence imaging of blood vessels and deeply-located intestinal tract of live mice based on organic dyes is achieved. The results show that organic fluorophore performs superb imaging ability in both superficial blood vessels and internal organs with high resolution and enhanced signal-to-background ratio in NIR-IIb region. We hope this groundbreakingly study will inspire further research on the evolution of pure organic NIR-IIb probes for in vivo imaging.</p>

scholarly journals A microrobotic system guided by photoacoustic computed tomography for targeted navigation in intestines in vivo

2019 ◽  
Vol 4 (32) ◽  
pp. eaax0613 ◽  
Author(s):  
Zhiguang Wu ◽  
Lei Li ◽  
Yiran Yang ◽  
Peng Hu ◽  
Yang Li ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Biomedical Applications ◽  
Near Infrared ◽  
Tissue Imaging ◽  
Infrared Light ◽  
Precise Control ◽  
Deep Imaging ◽  
Diverse Environment ◽  
Deep Tissue Imaging

Recently, tremendous progress in synthetic micro/nanomotors in diverse environment has been made for potential biomedical applications. However, existing micro/nanomotor platforms are inefficient for deep tissue imaging and motion control in vivo. Here, we present a photoacoustic computed tomography (PACT)–guided investigation of micromotors in intestines in vivo. The micromotors enveloped in microcapsules are stable in the stomach and exhibit efficient propulsion in various biofluids once released. The migration of micromotor capsules toward the targeted regions in intestines has been visualized by PACT in real time in vivo. Near-infrared light irradiation induces disintegration of the capsules to release the cargo-loaded micromotors. The intensive propulsion of the micromotors effectively prolongs the retention in intestines. The integration of the newly developed microrobotic system and PACT enables deep imaging and precise control of the micromotors in vivo and promises practical biomedical applications, such as drug delivery.

scholarly journals DNA-based nanocarriers to enhance the optoacoustic contrast of tumors in vivo

2020 ◽  
Author(s):  
James Joseph ◽  
Kevin N. Baumann ◽  
Alejandro Postigo ◽  
Laura Bollepalli ◽  
Sarah E. Bohndiek ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tumor Imaging ◽  
Signal Generation ◽  
Tissue Imaging ◽  
Optoacoustic Tomography ◽  
Synthetic Strategy ◽  
Deep Tissue Imaging ◽  
Spatio Temporal ◽  
Tumor Accumulation

AbstractOptoacoustic tomography (OT) enables non-invasive deep tissue imaging of optical contrast at high spatio-temporal resolution. The applications of OT in cancer imaging often rely on the use of molecular imaging contrast agents based on near infrared dyes to enhance contrast at the tumor site. While these agents afford excellent biocompatibility and minimal toxicity, they present limited optoacoustic signal generation capability and rapid renal clearance, which can impede their tumor imaging efficacy. In this work, a synthetic strategy to overcome these limitations utilizing biodegradable DNA-based nanocarrier (DNA-NC) platforms is introduced. DNA-NCs enable the incorporation of near infrared dyes (in this case, IRDye 800CW) at precise positions to enable fluorescence quenching and maximize optoacoustic signal generation. Furthermore, these DNA-NCs show a prolonged blood circulation compared to the native fluorophores, facilitating tumor accumulation by the Enhanced Permeability and Retention (EPR) effect. In vivo imaging of tumor xenografts in mice following intravenous administration of DNA-NCs revealed enhanced OT signals at 24h when compared to free fluorophores, indicating promise for this method to enhance the optoacoustic signal generation capability and tumor uptake of clinically relevant near infrared dyes.

scholarly journals Centimeter-Deep NIR-II Fluorescence Imaging with Nontoxic AIE Probes in Nonhuman Primates

Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Zonghai Sheng ◽  
Yaxi Li ◽  
Dehong Hu ◽  
Tianliang Min ◽  
Duyang Gao ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Nonhuman Primates ◽  
Histological Analysis ◽  
Blood Chemistry ◽  
Intravenous Dose ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Fluorescence Probes ◽  
Deep Tissue ◽  
Deep Tissue Imaging

Fluorescence probes with aggregation-induced emission (AIE) characteristics are of great importance in biomedical imaging with superior spatial and temporal resolution. However, the lack of toxicity studies and deep tissue imaging in nonhuman primates hinders their clinical translation. Here, we report the blood chemistry and histological analysis in nonhuman primates treated with AIE probes over tenfold of an intravenous dose of clinically used indocyanine green (ICG) during a study period of 36 days to demonstrate AIE probes are nontoxic. Furthermore, through bright and nontoxic AIE probes and fluorescence imaging in the second window (NIR-II, 1,000–1,700 nm), we achieve an unprecedented 1.5-centimeter-deep vascular imaging in nonhuman primates, breaking the current limitation of millimeter-deep NIR-II fluorescence imaging. Our important findings, i.e., nontoxic features of AIE probes and centimeter-deep NIR-II vascular imaging in nonhuman primates, may facilitate successful translation of AIE probes in clinical trials.

Evaluation of Red CdTe and Near Infrared CdHgTe Quantum Dots by Fluorescent Imaging

2008 ◽  
Vol 8 (3) ◽  
pp. 1155-1159 ◽  
Author(s):  
Jun Zhang ◽  
Junfeng Su ◽  
Li Liu ◽  
Yalou Huang ◽  
Ralph P. Mason
Keyword(s):  
Fluorescent Probes ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
Tissue Imaging ◽  
Antibody Conjugates ◽  
Deep Tissue Imaging ◽  
Membrane Antigens ◽  
Preclinical Animal Models

Non-invasive fluorescent imaging of preclinical animal models in vivo is a rapidly developing field with new emerging technologies and techniques. Quantum dot (QD) fluorescent probes with longer emission wavelengths in red and near infrared (NIR) emission ranges are more amenable to deep-tissue imaging, because both scattering and autofluorescence are reduced as wavelengths are increased. We have designed and synthesized red CdTe and NIR CdHgTe QDs for fluorescent imaging. We demonstrated fluorescent imaging by using CdTe and CdHgTe QDs as fluorescent probes both in vitro and in vivo. Both CdTe and CdHgTe QDs provided sensitive detection over background autofluorescence in tissue biopsies and live mice, making them attractive probes for in vivo imaging extending into deep tissues or whole animals. The studies suggest a basis of using QD-antibody conjugates to detect membrane antigens.

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