Ethanol Production from Oil Palm Trunk: A Combined Strategy Using an Effective Pretreatment and Simultaneous Saccharification and Cofermentation

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Agustin Krisna Wardani ◽  
Aji Sutrisno ◽  
Titik Nur Faida ◽  
Retno Dwi Yustina ◽  
Untung Murdiyatmo

Background. Oil palm trunk (OPT) with highly cellulose content is a valuable bioresource for bioethanol production. To produce ethanol from biomass, pretreatment is an essential step in the conversion of lignocellulosic biomass to fermentable sugars such as glucose and xylose. Several pretreatment methods have been developed to overcome biomass recalcitrance. In this study, the effects of different pretreatment methods such as alkali pretreatment, microwave-alkali, and alkaline peroxide combined with autoclave on the lignocellulosic biomass structure were investigated. Moreover, ethanol production from the treated biomass was performed by simultaneous saccharification and cofermentation (SSCF) under different temperatures, fermentation times, and cell ratios of Saccharomyces cerevisiae NCYC 479 and pentose-utilizing yeast, Pichia stipitis NCYC 1541. Results. Pretreatment resulted in a significant lignin removal up to 83.26% and cellulose released up to 80.74% in treated OPT by alkaline peroxide combined with autoclave method. Enzymatic hydrolysis of treated OPT resulted in an increase in fermentable sugar up to 93.22%. Optimization of SSCF by response surface method showed that the coculture could work together to produce maximum ethanol (1.89%) and fermentation efficiency (66.14%) under the optimized condition. Conclusion. Pretreatment by alkaline peroxide combined with autoclave method and SSCF process could be expected as a promising system for ethanol production from oil palm trunk and various lignocellulosic biomass.

2016 ◽  
Vol 6 (1) ◽  
pp. 773-779
Leonard Guimarães Carvalho ◽  
Luiz Felipe A. Modesto ◽  
Donato A. Gomes Aranda ◽  
Nei Pereira Jr

Brazil presents the world's largest potential for the production of palm oil due to nearly 75 million hectares of land suitable for palm culture and advantageous soil and climate. The biomass generated in the production of palm oil (palm pressed fiber, PPF) is mainly composed of lignocellulosic material that can be hydrolyzed into fermentable sugars for further conversion to ethanol. This work evaluated alkaline pretreatment of this palm oil residue and subsequent Simultaneous Saccharification and Fermentation (SSF), achieving a conversion of glucose to ethanol higher than 90% and a concentration equivalent to 22.40 g/L of the alcohol.  

2018 ◽  
Vol 61 (6) ◽  
pp. 1775-1782
Sun Min Kim ◽  
DoKyoung Lee ◽  
Santanu Thapa ◽  
Bruce S. Dien ◽  
Mike E. Tumbleson ◽  

Abstract. To examine the chemical composition and ethanol production of feedstocks grown on marginal lands, prairie cordgrass and switchgrass from waterlogged land, saline land, and saline water irrigated land were evaluated. Samples were pretreated using 1% w w-1 dilute acid at 160°C for 10 min, and simultaneous saccharification and cofermentation was conducted using industrial engineered . Samples grown on land irrigated with saline water had 2.8-fold higher total ash content compared to the other types of land, resulting in lower carbohydrate concentrations. Yeast fermented glucose and xylose simultaneously; almost all of the sugars were consumed, indicating that salts present in biomass ash did not inhibit yeast performance. Ethanol production from the waterlogged and saline lands was 2,500 to 4,700 L ha-1, which is comparable to that of samples grown on other agricultural lands. Prairie cordgrass and switchgrass grown on marginal lands could be potential feedstocks for cellulosic biofuel. Keywords: Irrigation, Marginal land, Prairie cordgrass, Saline, Simultaneous saccharification and cofermentation, Switchgrass, Waterlogging.

2014 ◽  
Vol 917 ◽  
pp. 80-86
Mohd Saman Siti Aisyah ◽  
Pacharakamol Petchpradab ◽  
Yoshimitsu Uemura ◽  
Suzana Yusup ◽  
Machi Kanna ◽  

Separate hydrolysis and fermentation (SHF) is the common process in producing ethanol from lignocellulosic biomass. Nowadays, simultaneous saccharification and fermentation (SSF) process has been seen as potential process for producing ethanol with shortens process time with higher yield of ethanol. Hence, in the current work, the utilization of empty fruit bunches (EFB) in SSF process was studied. In order to improve saccharification reactivity of EFB, hydrothermal pretreatment at 180 and 220 °C was used to pretreat EFB. The findings showed that SSF has the potential in producing ethanol from EFB.

2019 ◽  
Vol 59 (7) ◽  
pp. 744-753 ◽  
Wenxuan Zhao ◽  
Fuguang Zhao ◽  
Sitong Zhang ◽  
Qinglong Gong ◽  
Guang Chen

2014 ◽  
Vol 911 ◽  
pp. 307-313 ◽  
Siti Norsyarahah Che Kamarludin ◽  
Sandra Ubong ◽  
Nadzeerah Idris ◽  
Intan Suhada Azmi ◽  
Muhammad Syafiq Jainal ◽  

Ionic liquid (IL) has been shown to affect cellulose crystalline structure in lignocellulosic biomass (LB) during pretreatment. This research was carried out with two different experimental design involving IL to observe the effect of dissolution in IL on: (A) the crystallinity of cellulose and (B) the dissolution efficiency of LB. For experiment A, the types of IL used in this research were 1-ethyl-3-methylimidazolium Acetate [EMI[A, 1-allyl-3-methylimidazolium Chloride [AMI[C, 1-butyl-3-methylimidazolium Chloride [BMI[C and 1-ethyl-3-methylimidazolium Chloride [EMI[C. The crystallinity degree of LB was investigated before and after pretreatment with IL. The microcrystalline cellulose (MCC) was used as the simulated LB (cellulose content) was dissolved in IL and the crystallinity after the dissolution was analyzed. The temperature (70°C, 80°C, 90°C, 99°C) and concentration ratio of IL with volume/volume (v/v: 10%, 25%, 50%) were varied while the dissolution time remained constant. The crystallinity was analyzed by using Fourier Transform Infrared Spectroscopy (FTIR). The results showed that the dissolution temperature and IL concentration ratio affects the intensity of the FTIR peaks. In experiment B, the dissolution of LB with 1-butyl-3-methylimidazolium Chloride [BMI[C and 1-Ethyl-3-methylimidazolium Chloride [EMI[C as ILs were investigated. Four types of LB involved were Elaeis guineensis species of Oil Palm Trunk (OPT) and Oil Palm Frond (OPF) and Pennisetum purpureum species (elephant grass) originated from Taiwan and India. From the results obtained, the [BMI[C gave better dissolution to biomass.

2009 ◽  
Vol 55 (6) ◽  
pp. 346-355 ◽  
Kosuke Murai ◽  
Ryuichiro Uchida ◽  
Atsushi Okubo ◽  
Ryuichiro Kondo

2017 ◽  
Vol 41 (4) ◽  
pp. 467-477 ◽  
Kittipong Rattanaporn ◽  
Prapakorn Tantayotai ◽  
Theerawut Phusantisampan ◽  
Peerapong Pornwongthong ◽  
Malinee Sriariyanun

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