scholarly journals Effect of Platelet-Rich Plasma on Corneal Epithelial Healing after Phototherapeutic Keratectomy: An Intraindividual Contralateral Randomized Study

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Kazutaka Kamiya ◽  
Masahide Takahashi ◽  
Nobuyuki Shoji

Purpose. To assess the effect of platelet-rich plasma (PRP) on the healing response of the corneal epithelium in eyes undergoing phototherapeutic keratectomy (PTK). Methods. We prospectively examined 20 eyes of 10 patients undergoing bilateral PTK for granular corneal dystrophy or band keratopathy. Patients were randomly assigned to start topical administration of PRP ophthalmic suspension (PRP group) or artificial tears (control group) 4 times daily for 2 weeks. Immediately, 1, and 2 days, and 1 week after PTK, we quantitatively measured the staining area of the corneal epithelium, using slit-lamp photography. We also determined the subjective symptoms and the satisfaction, using the visual analogue system (VAS). Results. The staining area in the PRP group was significantly smaller than that in the control group on days 1 and 2 (Wilcoxon signed-rank test, p = 0.022 and p = 0.017 , respectively), but not on day 7 ( p = 0.317 ). The recovery rate of the corneal epithelium in the PRP group was significantly higher than that in the control group on days 1 and 2 ( p = 0.022 and p = 0.017 , respectively), but not on day 7 ( p = 0.317 ). We found no significant differences in pain ( p = 0.139 ), foreign body sensation ( p = 0.108 ), epiphora ( p = 1.000 ), or satisfaction ( p = 0.295 ), between the two groups. Postoperative complications did not occur in any of the eyes in the study. Conclusions. The PRP treatment was effective for enhancing corneal epithelial recovery in the early postoperative period, without significant adverse events, in post-PTK-treated eyes, suggesting that it may hold promise as one of the treatment options for treating such postsurgical patients.

2021 ◽  
Vol 8 (4) ◽  
pp. 365-374
Author(s):  
Tantut Susanto ◽  
Retno Purwandari ◽  
Emi Wuri Wuryaningsih ◽  
Hirohito Watanabe ◽  
Kana Kazawa ◽  
...  

Abstract Objective Maintaining blood pressure (BP) could improve the quality of life among farmers in agricultural health. The study aims to evaluate the effects of progressive muscular relaxation and stretching exercises (SEs) for BP in farmer subjects in rural areas. Methods A randomized controlled design was applied for this study. We performed a method, which is the combination of progressive muscle relaxation (PMR) and SEs for participants (30 in the control group and 60 in the intervention group). The intervention group self-practiced PMR and SEs through a video that providing instructions for 15 min. PMR practiced before going to sleeping in the night, and SEs practiced before going to farms in the morning per day for 3-months. Wilcoxon signed-rank test was performed to measure the difference between systolic blood pressure (SBP) and diastolic blood pressure (DBP) as one pre- and post-test comparison of baseline and 3 months data in control and intervention groups. Results There were no significant differences between SBP and DBP pre- and post-test in control group (P > 0.050). Meanwhile, there were significant differences in reducing SBP (M = 126.67; SD = 18.07; 95% CI = 120–147.5 mmHg) and DBP (M = 80.67; SD = 6.91; 95% CI = 80–90 mmHg) pre- and post-test combination of PMR and SEs in intervention group (P < 0.001). After 3-months of follow-up data, number type SBP and DBP still remained at the same levels of baseline and 3-month data in control group. While, there was an increased number of normal and prehypertension for SBP and DBP (10% vs. 10% and 20% vs. 31.6%) and reduced of hypertension stage I for SBP and DBP (30% vs. 41.6%). Conclusions This pilot study demonstrated effectively to reduce SBP and DBP among farmers using the combination of PMR and SEs in the agricultural health setting.


2020 ◽  
Vol 13 (4) ◽  
pp. 35-40
Author(s):  
S.V. Kotov ◽  
◽  
A.A. Nemenov ◽  
I.D. Boeva ◽  
◽  
...  

Introduction. The article analyzes the results of the clinical application of the herbal complex Renotinex®, which helps to normalize the function of the urinary tract, reduce pain, remove small stones from the kidneys and reduce the risk of recurrent stone formation. Materials and methods. At the University Clinic of Urology, Russian National Research Medical University named after N.I. Pirogov, a prospective, single-center, randomized study was carried out from November 2019 to March 2020. All patients underwent contact ureterolithotripsy. The patients were divided evenly into 2 groups: group A (n = 30) – patients receiving Renotinex® from the first day after surgery (within 1 month), and group B (n = 30) – a control group of patients not receiving specific therapy. Results. After 1 month of continuous use of the drug, there was an increase in the percentage of calculi discharge after contact ureterolithotripsy, a decrease in the severity of pain, normalization of the temperature curve and the absence of subfebrile condition in all patients. Conclusion. Renotinex® has a lithokinetic effect, its active ingredients contribute to the prevention of infectious and inflammatory complications of urolithiasis.


2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Zhaowei Meng ◽  
Jian Tan ◽  
Qing He ◽  
Mei Zhu ◽  
Xue Li ◽  
...  

We aimed to compare effectiveness of Wenxin Keli (WK) and sotalol in assisting sinus rhythm (SR) restoration from paroxysmal atrial fibrillation (PAF) caused by hyperthyroidism, as well as in maintaining SR. We randomly prescribed WK (18 g tid) or sotalol (80 mg bid) to 91 or 89 patients. Since it was not ethical not to give patients antiarrhythmia drugs, no control group was set. Antithyroid drugs were given to 90 patients (45 in WK group, 45 in sotalol group);131I was given to 90 patients (46 in WK group, 44 in sotalol group). Three months later, SR was obtained in 83/91 or 80/89 cases from WK or sotalol groups(P=0.762). By another analysis, SR was obtained in 86/90 or 77/90 cases from131I or ATD groups(P=0.022). Then, we randomly assigned the successfully SR-reverted patients into three groups: WK, sotalol, and control (no antiarrhythmia drug was given) groups. After twelve-month follow-up, PAF recurrence happened in 1/54, 2/54, and 9/55 cases, respectively. Log-Rank test showed significant higher PAF recurrent rate in control patients than either treatment(P=0.06). We demonstrated the same efficacies of WK and sotalol to assist SR reversion from hyperthyroidism-caused PAF. We also showed that either drug could maintain SR in such patients.


QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
A A M E A Rezk ◽  
A Assaf ◽  
M Gamil ◽  
T Badran

Abstract Introduction The corneal epithelium is stratified epithelium that is continuously renewed and provides the frontline of defence against invading ocular pathogens and a smooth refractive surface essential for vision. In the absence of a contact lens, pre-existing ocular trauma or disease, the epithelium maintains an inaccessible defence against attacks from pathogenic microorganisms, affording a high level of resistance against microbial invasion. Aim The aim of this study is to determine the effect of chronic use of contact lens on corneal epithelium thickness using anterior segment optical coherence topography as a non- invasive diagnostic accurate measure. Patients and Methods 30 eyes of control subjects who don’t wear contact lens along their life aged between 15-45 years ,where compared with similar age group of 30 eyes of chronic contact lens users; who wear contact lens every day not less than 8 hours per day for along time interval between 6 months up to 5 years. Results The average corneal epithelial thickness in the central, paracentral and midperipheral zones was 47.767 ± 5.550 µm, 46.267 ± 5.644 µm, 44.300 ± 4.858 µm, respectively, in chronic soft contact lens users; and 49.800 ± 3.316 µm, 49.200 ± 3.367 µm, 45.733 ± 2.333 µm ,in control group who had never worn contact lens. There were insignificant thinning of corneal epithelium of soft contact group compared to control group regarding the average corneal epithelial thickness in those 3 zones. In all the 8 sectors of corneal epithelium of paracentral zone and midperipheral zones there is insignificant thinning between the two groups except for the inferior temporal sector in the paracentral zone and mid peripheral zone, which shows significant thinning in soft contact lens group compared to control group. The corneal epithelial thickness in the inferior temporal sector of paracentral and mid peripheral zones was 46.333 ± 5.677 µm, 44.933 ± 4.813 µm, respectively, in chronic soft contact lens users compared to 48.767 ± 3.266 µm, 46.900 ± 2.510 µm in control group (p = 0.046, p = 0.052, respectively). Conclusion AS-OCT helps us to evaluate the corneal epithelium of contact lens users, which could be very useful in corneal refractive surgeries in patients depending on contact lens in their lives as a comfortable refractive aid, It is necessary to do AS-OCT hand on hand with pentacam in patients underwhelming refractive surgery to give a proper assessment to their corneal epithelium before determining which type of refractive surgery suits them.


2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Luigi Ambrosone ◽  
Germano Guerra ◽  
Mariapia Cinelli ◽  
Mariaelena Filippelli ◽  
Monica Mosca ◽  
...  

Different liposomal formulations were prepared to identify those capable of forming eyedrops for corneal diseases. Liposomes with neutral or slightly positive surface charge interact very well with the cornea. Then these formulations were loaded with verbascoside to heal a burn of corneal epithelium induced by alkali. The cornea surface affected involved in wound was monitored as a function of time. Experimental results were modeled by balance equation between the rate of healing, due to the flow of phenylpropanoid, and growth of the wound. The results indicate a latency time of only three hours and furthermore the corneal epithelium heals in 48 hours. Thus, the topical administration of verbascoside appears to reduce the action time of cells, as verified by histochemical and immunofluorescence assays.


2020 ◽  
Vol 10 (23) ◽  
pp. 8312
Author(s):  
Przemysław Krakowski ◽  
Robert Karpiński ◽  
Ryszard Maciejewski ◽  
Józef Jonak ◽  
Andrzej Jurkiewicz

Purpose: The purpose of this study was to estimate the effect of platelet-rich plasma (PRP) augmentation in microfractures of chondral lesions in elderly individuals. Methods: 29 patients with knee osteoarthritis were enrolled in the single-blinded randomized study. The study group consisted of 16 patients and 13 were in the control group. All surgeries were performed in the same manner. The PRP injection was made in a dry arthroscopy directly over the microfractures. The evaluation was performed preoperatively on the 6th, 12th, and 24th week following the surgery. WOMAC and Lysholm questionnaires were utilized. Results: Microfracturation showed WOMAC improvement in the PRP group (p = 0.0012) and in the control group (p = 0.0042). No statistically significant differences between the two groups (p = 0.698) have been found. Clinical improvement was noted in the first six weeks after which the effect lasted. In addition, Lysholm score showed no significant differences at the end of the follow-up between both groups (p = 0.941). Conclusions: Arthroscopic microfracture improves motor function and reduces pain in patients over 50 years old. PRP augmentation of the procedure is safe, and can accelerate and prolong the therapeutic effect of treatment. Excellent effects of the procedure are observed in the first six weeks, after which the improvement lasts.


2021 ◽  
Vol 39 (15_suppl) ◽  
pp. e14027-e14027
Author(s):  
Ivan A. Popov ◽  
Oleg I. Kit ◽  
Alla I. Shikhlyarova ◽  
Elena M. Frantsiyants ◽  
Eduard E. Rostorguev ◽  
...  

e14027 Background: The existing modern standards of combination treatment of HGG patients do not provide recovery and a long-term favorable prognosis, and the increasong incidence of HGG determines the need for additional effective technologies for anticancer and decongestant therapy. One of such methods involves TMS, and we have reported its preliminary assessment earlier (DOI: 10.1200/JCO.2020.38.15_suppl.2545). In this study, we continued the observation to examine the survival of patients. Methods: Patients with HGG received combination treatment: stage 1 – surgical removal of tumors within visible unaltered tissues; stage 2 – radiation therapy (the Varian Novalis linear accelerator) to the bed of the removed tumor, single boost dose 2 Gy, total boost dose 60 Gy; stage 3 – multi-course chemotherapy: temozolomide 150 mg/m² on days 1-5 with a 23-day interval. Starting from the second day after surgery, patients of group 1 (n = 25) received 10 TMS sessions, and during radiotherapy – 15 TMS sessions. Patients of group 2 (n = 25) received combination treatment without TMS. 6 and 12 months after the surgery, survival of patients was assessed with the Kaplan-Meier method and the Log-Rank test. Results: After 6 months of the follow-up, the survival of patients in group 1 remained at 100%, while in the control group it decreased to 88.8±8.7%. The difference in the 1-year overall survival was even more pronounced: in group 1, it was 68.5±10.4%, exceeding the value in group 2 (52.0±7.5%.) The differences were statistically significant (Log-Rank test p = 0.001). Conclusions: The results confirm the effectiveness of accompanying TMS in the early postoperative period, as well as at the stage of radiation therapy. The undoubted effectiveness of the considered techniques makes it expedient to include this type of treatment in the combination therapy for HGG patients. The reported study was funded by RFBR, project number № 19-315-90082\19.


QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Fatma El-Hennawi ◽  
Hazem Rashed ◽  
Reham Fawzy ◽  
Kholoud Selim

Abstract Background Cataract surgery is traumatic to the corneal epithelium,scarring and opacity is the commonest cause of blindness. Objective To study the corneal epithelial thickness in different corneal conditions using anterior segment optical coherence tomography (AS-OCT). Patients and Methods A case-control study including 80 eyes divided equally into 4 groups; group 1:controls,group 2:corneal scarring ,group 3:cataract patients and group 4:pterygium. using AS-OCT epithelial mapping to document changes in epithelial thickness in controls, cataract patients pre and 1 month after phacoemulsification, patients with corneal scarring and patients with pterygium. Results In phacoemulsification group; we found that epithelial thickness became thinner in area (0_2) and thicker in area (7_9) mm in the map with no significant change in areas (2_5),(5_7) mm in the map. In corneal scarring group; we found that epithelial thickness became thicker compared to control group in all zones. In pterygium group; we found that epithelial thickness became thicker compared to control group in, areas (2_5), (5_7) & (7_9) mm in the map with no significant change in area (0_2) mm in the map. Conclusion The corneal epithelium thickness becomes thinner or thicker to compensate for changes in stromal thickness.


Author(s):  
E. N. Simakova ◽  
O. V. Stenkova

Introduction. Glaucoma is one of the most significant eye diseases. It is often diagnosed, not always amenable to therapy, and can lead to a complete loss of visual functions. In recent years, the method of osteopathic correction has become widespread as one of the effective methods of treatment and rehabilitation of patients with pathologies of various body systems. In the pathogenesis of glaucoma, it is customary to distinguish a dystrophic concept, which considers primary open-angle glaucoma as a result of dystrophic changes in the connective tissue, as well as in the endothelial lining of the trabeculae and Schlemm′s canal, especially destructive changes in mitochondria and the alteration of their functional activity. A vascular concept is also distinguished. According to this concept, the central link in the pathogenesis of glaucoma is circulatory disorder in the ciliary vessels, ocular artery, and major vessels of the head and neck, it can be assumed that osteopathic correction in the treatment of patients with open-angle glaucoma will be pathogenetically substantiated and will have a positive effect on intraocular pressure and trophicity of the optic nerve. The goal of research — to study the influence of in osteopathic correction on the nature of unoperated glaucoma (stage IIA) and to substantiate the possibility of using osteopathic correction in the complex treatment of patients with this pathology.Materials and methods. A prospective controlled randomized study was conducted at 52 city polyclinics, branch 3, Moscow, from January 2018 to January 2019. 40 patients (70 eyes) aged 50 to 75 years with primary open-angle glaucoma IIA stage were examined. At this stage of the disease, patients most often seek medical care and the issue of conservative management is primarily considered. All patients were divided into two groups of 20 people: the main group and the control group. The treatment in the main group included hypotensive drug therapy and osteopathic correction. Patients of the control group received only drug therapy. All patients underwent ophthalmic (visometry, tonometry, perimetry) and osteopathic examination twice: before the treatment and after 3 months.Results. For patients with primary open-angle IIA non-operated glaucoma, regional (most often regions of the head, neck, dura mater) and local (abdominal diaphragm, iliac bones, hip and knee joints) somatic dysfunctions were the most typical. In the main group a statistically significant decrease in the frequency and severity of dysfunctions at all levels was stated. Also, in patients receiving osteopathic correction, a significant decrease in the level of intraocular pressure and perimetric indices was noted. In patients of the control group, no reliable changes in these indicators were obtained.Conclusion. The results obtained indicate that osteopathic correction is clinically effective in the complex treatment of patients with primary open-angle II A glaucoma.


2020 ◽  
Vol 17 ◽  
Author(s):  
Van-An Duong ◽  
Jeeyun Ahn ◽  
Na-Young Han ◽  
Jong-Moon Park ◽  
Jeong-Hun Mok ◽  
...  

Background: Diabetic Retinopathy (DR), one of the major microvascular complications commonly occurring in diabetic patients, can be classified into Proliferative Diabetic Retinopathy (PDR) and Non-Proliferative Diabetic Retinopathy (NPDR). Currently available therapies are only targeted for later stages of the disease in which some pathologic changes may be irreversible. Thus, there is a need to develop new treatment options for earlier stages of DR through revealing pathological mechanisms of PDR and NPDR. Objective: The purpose of this study was to characterize proteomes of diabetic through quantitative analysis of PDR and NPDR. Methods: Vitreous body was collected from three groups: control (non-diabetes mellitus), NPDR, and PDR. Vitreous proteins were digested to peptide mixtures and analyzed using LC-MS/MS. MaxQuant was used to search against the database and statistical analyses were performed using Perseus. Gene ontology analysis, related-disease identification, and protein-protein interaction were performed using the differential expressed proteins. Results: Twenty proteins were identified as critical in PDR and NPDR. The NPDR group showed different expressions of kininogen-1, serotransferrin, ribonuclease pancreatic, osteopontin, keratin type II cytoskeletal 2 epidermal, and transthyretin. Also, prothrombin, signal transducer and activator of transcription 4, hemoglobin subunit alpha, beta, and delta were particularly up-regulated proteins for PDR group. The up-regulated proteins related to complement and coagulation cascades. Statherin was down-regulated in PDR and NPDR compared with the control group. Transthyretin was the unique protein that increased its abundance in NPDR compared with the PDR and control group. Conclusion: This study confirmed the different expressions of some proteins in PDR and NPDR. Additionally, we revealed uniquely expressed proteins of PDR and NPDR, which would be differential biomarkers: prothrombin, alpha-2-HS-glycoprotein, hemoglobin subunit alpha, beta, and transthyretin.


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