scholarly journals Cytotoxic and Antimigration Activity of Etlingera alba (A.D.) Poulsen Rhizome

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
W. Wahyuni ◽  
Ajeng Diantini ◽  
Mohammad Ghozali ◽  
Anas Subarnas ◽  
Euis Julaeha ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Triple Negative ◽  
Chemical Components ◽  
Active Fraction ◽  
New Agents ◽  
Antimetastatic Activity ◽  
Cell Assay ◽  
Ic50 Value ◽  
And Migration ◽  
Chemical Contents

Etlingera alba is one of the Etlingera plants that might have anticancer activity. This study aims to investigate the cytotoxic and antimetastatic activity of E. alba rhizome fractions and migration cell assay against MDA-MB-231 cell lines, which are used for triple-negative breast cancer (TNBC) treatment assay. The cytotoxic activity was assayed using CCK-8 assay, while the antimetastatic was assayed using migration cell assay for the fractions A–F. They were followed by LCMS/MS profiling to determine the chemical contents in the most active fraction. According to results obtained, fraction B was the most active fraction for cytotoxic activity with an IC50 value of 65.43 μg/mL, while fraction E was the most active fraction for antimetastasis activity against migration rate doses of 50, 100, and 200 ppm which were 6.80, 3.66, and 3.00%, respectively. Several compounds in fraction B, such as rengyolone, licochalcone A, sugiol, and spinasterol, might have been known to have activity against cancer cells, as well as aschantin and lirioresinol B dimethyl ether from fraction E. In conclusion, the chemical components from E. alba rhizome fractions provided potency for discovering new agents for cancer treatment, specifically for TNBC.

scholarly journals Short Communication: The most active fraction of red turi flowers (Sesbania grandiflora) on the cytotoxic activity of HepG2 cells

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Sulasmi Sulasmi ◽  
OKID P. ASTIRIN ◽  
TETRI WIDIYANI
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Hepg2 Cells ◽  
Chemical Compounds ◽  
Ethyl Acetate Fraction ◽  
Flavonoid Compound ◽  
Active Fraction ◽  
Water Fraction ◽  
Sesbania Grandiflora ◽  
Ic50 Value

Abstract. Sulasmi, Astirin OP, Widiyanti T. 2020. Short Communication: The most active fraction of red turi flowers (Sesbania grandiflora) on the cytotoxic activity of HepG2 cells. Nusantara Bioscience 12: 68-72. Red turi flowers (Sesbania grandiflora L.Pers) contain many chemical compounds with high bioactivity. One of the chemical compounds is flavonoid compound. Quercetin is a class of flavonoid compound. This compound is obtained by extraction with methanol as a solvent then it is partitioned by water and ethyl acetate as a solvent. The fraction obtained is given phytochemical tests, which is further test using Thin Layer Chromatography (TLC) to detect the quercetin compounds. The level of flavonoid is determined using UV Vis spectrophotometer. Water and ethyl acetate fractions are tested cytotoxically using the MTT method (3-(4,5-dimethyltiazol-2-il)-2,5-diphenyltetrazolium bromide) with ELISA Microplate Reader. This study aims to determine the most active fraction of red turi flowers on the cytotoxic activity of HepG2 cells, which are model cells for liver cancer. The results of water fraction do not contain quercetin compounds, with IC50 value of 961.11µg/mL. The ethyl acetate fraction contains quercetin compounds with total flavonoid equivalent quercetin of 1.76% b/b and IC50 value of 149.15µg/mL. Ethyl acetate fraction is more active than the water fraction with moderate cytotoxicity.

Quercetin Impairs HuR-Driven Progression and Migration of Triple Negative Breast Cancer (TNBC) Cells

2021 ◽  
pp. 1-14
Author(s):  
Sheikh Mohammad Umar ◽  
Sushmita Patra ◽  
Akanksha Kashyap ◽  
Arundhathi Dev J R ◽  
Lalit Kumar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
And Migration

scholarly journals The Potential Prognostic Role of Oligosaccharide-Binding Fold-Containing Protein 2A (OBFC2A) in Triple-Negative Breast Cancer

2021 ◽  
Vol 11 ◽  
Author(s):  
Qianxue Wu ◽  
Xin Tang ◽  
Wenming Zhu ◽  
Qing Li ◽  
Xiang Zhang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cancer Cells ◽  
Candidate Genes ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
G1 Phase ◽  
And Migration

BackgroundPatients with triple-negative breast cancer (TNBC) have poor overall survival. The present study aimed to investigate the potential prognostics of TNBC by analyzing breast cancer proteomic and transcriptomic datasets.MethodsCandidate proteins selected from CPTAC (the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium) were validated using datasets from METABRIC (Molecular Taxonomy of Breast Cancer International Consortium). Kaplan-Meier analysis and ROC (receiver operating characteristic) curve analysis were performed to explore the prognosis of candidate genes. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis were performed on the suspected candidate genes. Single-cell RNA-seq (scRNA-seq) data from GSE118389 were used to analyze the cell clusters in which OBFC2A (Oligosaccharide-Binding Fold-Containing Protein 2A) was mainly distributed. TIMER (Tumor Immune Estimation Resource) was used to verify the correlation between OBFC2A expression and immune infiltration. Clone formation assays and wound healing assays were used to detect the role of OBFC2A expression on the proliferation, invasion, and migration of breast cancer cells. Flow cytometry was used to analyze the effects of silencing OBFC2A on breast cancer cell cycle and apoptosis.ResultsSix candidate proteins were found to be differentially expressed in non-TNBC and TNBC groups from CPTAC. However, only OBFC2A was identified as an independently poor prognostic gene marker in METABRIC (HR=3.658, 1.881-7.114). And OBFC2A was associated with immune functions in breast cancer. Biological functional experiments showed that OBFC2A might promote the proliferation and migration of breast cancer cells. The inhibition of OBFC2A expression blocked the cell cycle in G1 phase and inhibited the transformation from G1 phase to S phase. Finally, downregulation of OBFC2A also increased the total apoptosis rate of cells.ConclusionOn this basis, OBFC2A may be a potential prognostic biomarker for TNBC.

Antiglycolytic Activities of Strobilanthes crispus Active Fraction and its Bioactive Components on Triple-Negative Breast Cancer Cells In Vitro

2021 ◽  
Vol 21 ◽  
Author(s):  
Siti N.H. Muhammad ◽  
Nik S. Yaacob ◽  
Nur A.M. Safuwan ◽  
Agustine N. Fauzi
Keyword(s):  
Breast Cancer ◽  
Glucose Uptake ◽  
Cancer Cells ◽  
Mtt Assay ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Lactate Concentration ◽  
Active Fraction ◽  
Bioactive Components ◽  
Strobilanthes Crispus

Background: Survival and progression of cancer cells are highly dependent on aerobic glycolysis. Strobilanthes crispus has been shown to have promising anticancer effects on breast cancer cells. The involvement of the glycolysis pathway in producing these effects is unconfirmed, thus further investigation is required to elucidate this phenomenon. Objective: This study aims to determine the effect of S. crispus active fraction (F3) and its bioactive components on glycolysis in triple-negative breast cancer cells (MDA-MB-231). Methods: This study utilizes F3, lutein, β-sitosterol, and stigmasterol to be administered in MDA-MB-231 cells for measurement of antiglycolytic activities through cell poliferation, glucose uptake, and lactate concentration assays. Cell proliferation was assessed by MTT assay of MDA-MB-231 cells after treatment with F3 and its bioactive components lutein, β-sitosterol, and stigmasterol. The IC50 value in each compound was determined by MTT assay to be used in subsequent assays. The determination of glucose uptake activity and lactate concentration were quantified using fluorescence spectrophotometry. Results: Antiproliferative activities were observed for F3 and its bioactive components, with IC50 values of 100 µg/mL (F3), 20 µM (lutein), 25 µM (β-sitosterol), and 90 μM (stigmasterol) in MDA-MB-231 cells at 48 h. The percentage of glucose uptake and lactate concentration in MDA-MB-231 cells treated with F3, lutein, or β sitosterol were significantly lower than those observed in the untreated cells in a time-dependent manner. However, treatment with stigmasterol decreased the concentration of lactate without affecting the glucose uptake in MDA-MB-231 cells. Conclusion: The antiglycolytic activities of F3 on MDA-MB-231 cells are attributed to its bioactive components.

scholarly journals MicroRNA-455-3p promotes invasion and migration in triple negative breast cancer by targeting tumor suppressor EI24

Oncotarget ◽  
2016 ◽  
Vol 8 (12) ◽  
pp. 19455-19466 ◽  
Author(s):  
Zhishuang Li ◽  
Qingyong Meng ◽  
Aifeng Pan ◽  
Xiaojuan Wu ◽  
Jingjing Cui ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Suppressor ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Invasion And Migration ◽  
And Migration

Differential Chemotactic Behavior of Developing T Cells in Response to Thymic Chemokines

Blood ◽  
1998 ◽  
Vol 91 (12) ◽  
pp. 4434-4443 ◽  
Author(s):  
Chang H. Kim ◽  
Louis M. Pelus ◽  
John R. White ◽  
Hal E. Broxmeyer
Keyword(s):  
T Cells ◽  
Triple Negative ◽  
Selection Process ◽  
Chemotactic Activity ◽  
Double Positive ◽  
Single Positive ◽  
And Migration ◽  
Late Stages ◽  
Immature Thymocytes ◽  
Chemotactic Behavior

Abstract Differentiation-dependent thymocyte migration in the thymus may be important for T lymphopoiesis and might be regulated by thymic chemoattractants. We examined modulation of chemotactic responsiveness of thymocyte subsets during their early to late stages of development in response to 2 thymus-expressed chemokines, SDF-1 and CKβ-11/MIP-3β/ELC. SDF-1 shows chemotactic preference for immature thymocytes (subsets of triple negative thymocytes and double positive [DP] subset) over mature single positive (SP) thymocytes. CKβ-11/MIP-3β/ELC shows low chemotactic activity on the immature thymocytes, but it strongly attracts mature SP thymocytes, effects opposite to that of SDF-1. SDF-1–dependent chemoattraction of immature thymocytes is not significantly desensitized by a negative concentration gradient of CKβ-11/MIP-3β/ELC, and chemoattraction of mature SP thymocytes to CKβ-11/MIP-3β/ELC is not antagonized by SDF-1, demonstrating that these two chemokines have different chemoattractant preferences for thymocyte subsets and would probably not inhibit each other's chemotaxis in the event of microenvironmental coexpression. The chemotactic responsiveness of thymocytes and mature T cells to the 2 chemokines is respectively enhanced after selection process and migration to the spleen. These studies demonstrate the presence of thymocyte chemoattractants with differential chemotactic preference for thymocytes, a possible mechanism for thymocyte migration in the thymus.

scholarly journals Strictinin, a novel ROR1-inhibitor, represses triple negative breast cancer survival and migration via modulation of PI3K/AKT/GSK3ß activity

PLoS ONE ◽  
2019 ◽  
Vol 14 (5) ◽  
pp. e0217789 ◽  
Author(s):  
Norman Fultang ◽  
Abhinav Illendula ◽  
Brian Chen ◽  
Chun Wu ◽  
Subash Jonnalagadda ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Cancer Survival ◽  
Breast Cancer Survival ◽  
And Migration
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