Different Parts of the Chicken Embryo Egg Improve D-Galactose-Induced Aging in a Mice Model

Chick (CE) or duck embryo eggs are known for nutritional supplement foods in traditional East countries for physical fitness enhancement and postpartum conditioning for many years. In this study, we evaluated the effects of different parts of the 10-day CE (embryo: CEr, yolk: CEw, and chorioallantoic membrane: CEp) on the antifatigue and antiaging activities in a D-galactose- (D-gal) induced aging mice model. The results showed CEp obviously increased the muscle weight and the liver and muscle glycogen content and enhanced exercise performance. In the antiaging assay, CEp significantly increased the activity of superoxide dismutase (SOD) and Glutathione Peroxidase (GPx). Moreover, the immunohistochemistry results of NRF-2 and HO-1 were also detected in the livers of mice in the D-gal/CEp group. The only partially potential such as CEr might improve OFT function with TG level, and CEw had strange grip strength. Therefore, we suggest that CEp has a potent antifatigue ability and could minimize the occurrence of age-associated disorders, more than other parts of the 10 days chicken embryo egg.

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Antioxidant Effect of MnTE-2-PyP on Lung in Asthma Mice Model

The Scientific World JOURNAL ◽

10.1100/2012/379360 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 6

Author(s):

Lyudmil Terziev ◽

Violeta Dancheva ◽

Veneta Shopova ◽

Galya Stavreva

Keyword(s):

Superoxide Dismutase ◽

Glutathione Peroxidase ◽

Control Group ◽

Mice Model ◽

Antioxidant Defence ◽

Group 4 ◽

Antioxidant Defence System ◽

Group 3 ◽

Group 2 ◽

Group 1

Aim. To investigate the effects of MnTE-2-PyP on some markers of antioxidant defence system in asthma mice model.Material and Methods. The animals were divided into four groups: group 1, controls; group 2, injected with ovalbumin, group 3, treated with MnTE-2-PyP, and group 4, treated with ovalbumin and MnTE-2-PyP. The activities of superoxide dismutase, catalase, glutathione peroxidase and nonprotein sulfhydryl groups content (NPSH) were determined in lung homogenate.Results. The activities of superoxide dismutase and catalase in group 2 decreased significantly as compared to control group. The decrease of the same enzymes in group 4 was lower and significant as compared to group 2. Changes in the glutathione peroxidase activity showed a similar dynamics. The NPSH groups content decreased in group 2. In group 4 this decrease was relatively lower as compared to group 2.Conclusions. The application of MnTE-2-PyP mitigated the effects of oxidative stress in asthma mice model.

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State of the enzyme link of antioxidant protection in boys with hypoandrogenism

Problems of Uninterrupted Medical Training and Science ◽

10.31071/promedosvity2020.04.032 ◽

2020 ◽

Vol 40 (4) ◽

pp. 32-36

Author(s):

L. K. Parkhomenko ◽

◽

L. A. Strashok ◽

S. I. Turchina ◽

G. V. Kosovtsova ◽

...

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Free Radical ◽

Glutathione Peroxidase ◽

Free Radical Oxidation ◽

Conjugated Dienes ◽

Delayed Puberty ◽

Control Group ◽

Antioxidant Protection ◽

Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

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Glutathione peroxidase compensates for the hypersensitivity of Cu,Zn-superoxide dismutase overproducers to oxidant stress.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)42068-0 ◽

1994 ◽

Vol 269 (3) ◽

pp. 1606-1609 ◽

Cited By ~ 1

Author(s):

P. Amstad ◽

R. Moret ◽

P. Cerutti

Keyword(s):

Superoxide Dismutase ◽

Glutathione Peroxidase ◽

Oxidant Stress

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Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress

Zygote ◽

10.1017/s0967199419000467 ◽

2019 ◽

Vol 27 (6) ◽

pp. 432-435

Author(s):

Thais Rose dos Santos Hamilton ◽

Gabriela Esteves Duarte ◽

José Antonio Visintin ◽

Mayra Elena Ortiz D’Ávila Assumpção

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Antioxidant Enzymes ◽

Heat Stress ◽

Glutathione Peroxidase ◽

Cell Types ◽

Treated Group ◽

Oxidative Balance ◽

Testicular Cells

SummaryLong-term heat stress (HS) induced by testicular insulation generates oxidative stress (OS) on the testicular environment; consequently activating antioxidant enzymes such as superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx). The aim of this work was to immunolocalize antioxidant enzymes present in different cells within the seminiferous tubule when rams were submitted to HS. Rams were divided into control (n = 6) and treated group (n = 6), comprising rams subjected to testicular insulation for 240 h. After the testicular insulation period, rams were subjected to orchiectomy. Testicular fragments were submitted to immunohistochemistry for staining against SOD, GR and GPx enzymes. We observed immunolocalization of GPx in more cell types of the testis after HS and when compared with other enzymes. In conclusion, GPx is the main antioxidant enzyme identified in testicular cells in an attempt to maintain oxidative balance when HS occurs.

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Effect of Cross-Sex Hormonal Replacement on Antioxidant Enzymes in Rat Retroperitoneal Fat Adipocytes

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/1527873 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 5

Author(s):

Israel Pérez-Torres ◽

Verónica Guarner-Lans ◽

Alejandra Zúñiga-Muñoz ◽

Rodrigo Velázquez Espejel ◽

Alfredo Cabrera-Orefice ◽

...

Keyword(s):

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Antioxidant Enzymes ◽

Glutathione Peroxidase ◽

Glutathione Reductase ◽

Enzymatic Activities ◽

Glutathione S Transferase ◽

Control Groups ◽

Intact Female ◽

Hormonal Replacement

We report the effect of cross-sex hormonal replacement on antioxidant enzymes from rat retroperitoneal fat adipocytes. Eight rats of each gender were assigned to each of the following groups: control groups were intact female or male (F and M, resp.). Experimental groups were ovariectomized F (OvxF), castrated M (CasM), OvxF plus testosterone (OvxF + T), and CasM plus estradiol (CasM + E2) groups. After sacrifice, retroperitoneal fat was dissected and processed for histology. Adipocytes were isolated and the following enzymatic activities were determined: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). Also, glutathione (GSH) and lipid peroxidation (LPO) were measured. In OvxF, retroperitoneal fat increased and adipocytes were enlarged, while in CasM rats a decrease in retroperitoneal fat and small adipocytes are observed. The cross-sex hormonal replacement in F rats was associated with larger adipocytes and a further decreased activity of Cu-Zn SOD, CAT, GPx, GST, GR, and GSH, in addition to an increase in LPO. CasM + E2exhibited the opposite effects showing further activation antioxidant enzymes and decreases in LPO. In conclusion, E2deficiency favors an increase in retroperitoneal fat and large adipocytes. Cross-sex hormonal replacement in F rats aggravates the condition by inhibiting antioxidant enzymes.

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