scholarly journals Identification and Characterisation of Seed-Borne Fungal Pathogens Associated with Maize (Zea mays L.)

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
M. L. Goko ◽  
J. C. Murimwa ◽  
E. Gasura ◽  
J. T. Rugare ◽  
E. Ngadze
Keyword(s):  
Zea Mays ◽  
Fungal Pathogens ◽  
Research Study ◽  
Zea Mays L ◽  
Aspergillus Parasiticus ◽  
Block Design ◽  
Fungal Species ◽  
Aspergillus Tamarii ◽  
Maize Seeds ◽  
Plating Method

A research study was conducted to identify and characterise seed-borne fungal pathogens associated with maize (Zea mays L.) in storage. Seed-borne fungal pathogenic infections of maize were studied using seed samples collected from Gokwe South District in Zimbabwe. The agar plating method using PDA medium was used to detect fungal pathogens on the maize seeds. A total of 150 treatments were used for this experiment, which were replicated three times in a randomised complete block design (RCBD). Analysis of the grain showed the presence of Fusarium moniliforme, Rhizopus stolonifer, Penicillium citrinum, and mostly Aspergillus species, namely, Aspergillus flavus, Aspergillus parasiticus, Aspergillus niger, and Aspergillus tamarii. Significant differences ( p  < 0.05) between treatments were detected for the pathogens. A total of ten samples were used for mycotoxin determination, and all of them were 100% positive with aflatoxin total, zearalenone, fumonisin, and deoxynivalenol (DON) having an average of 0.255 ppb, 2.425 ppb, 2.65 ppb, and 0.07 ppb, respectively. The present study showed that most grain samples are contaminated with different species of fungi with mycotoxigenic potential. The data on the diversity and magnitude of pathogen infection by fungal species will have a significant effect even at the regional level for predicting the extent of pre- and postinfections. Measures to reduce mycotoxin contamination are needed for maize grains.

QUALIDADE FISIOLÓGICA E EXPRESSÃO DE ALFA-AMILASE EM SEMENTES DE MILHO PRODUZIDAS EM CONDIÇÕES DE ESTRESSE SALINO E HÍDRICO

2018 ◽  
Vol 17 (1) ◽  
pp. 37 ◽  
Author(s):  
RAFAEL PAREIRA DINIZ ◽  
IOLANDA VILELA VON PINHO ◽  
BRUNO DA COSTA PANIAGO ◽  
EDILA VILELA DE REZENDE VON PINHO ◽  
HELOISA OLIVEIRA DOS SANTOS ◽  
...  
Keyword(s):  
Zea Mays ◽  
Salt Stress ◽  
Zea Mays L ◽  
Block Design ◽  
Stress Conditions ◽  
Maturity Stage ◽  
Development Stages ◽  
Maize Seeds ◽  
Physiological Analysis ◽  
Seeds Germination

RESUMO - Durante o processo de produção de sementes, estresses abióticos como salinidade do solo e déficit hídrico são fatores determinantes e que influenciam no estabelecimento e desenvolvimento uniforme do estande em campo. Nesse sentido, cultivares tolerantes a altas concentrações salinas nestas fases são fundamentais nos programas de melhoramento. Diante disto, objetivou-se com a presente pesquisa verificar a influência da produção de sementes sob condições de estresse hídrico e salino na germinação e no vigor, assim como na atividade da enzima α-amilase em sementes de milho. Para isso, foram conduzidos dois experimentos para a produção de sementes de milho, um em condições de estresse salino e o outro sob condições de déficit hídrico. Os experimentos foram conduzidos em delineamento de blocos completos casualisados, em esquema de parcelas subdivididas. As sementes colhidas em diferentes estádios de desenvolvimento, determinados pela linha de leite (LL), foram submetidas a análises fisiológicas e de vigor, bem como determinou-se a expressão da enzima α-amilase por eletroforese. Diante dos resultados foi possível concluir que a germinação e o vigor das sementes produzidas em solos com condutividade de 3 dS m-1 não são afetados pela salinidade, e o desenvolvimento das plantas em condições de estresse hídrico e salino não afetou a atividade da enzima α-amilase em sementes colhidas na LL5. Palavras-chave: Zea mays L., eletroforese, estádios de desenvolvimento. PHYSIOLOGICAL QUALITY AND ALPHA-AMYLASE EXPRESSION OF MAIZE SEEDS IN CONDITIONS OF SALT AND WATER STRESS ABSTRACT - During seed production process, abiotic stresses such as soil salinity and drought are determining factors that influence the establishment and development of uniform stand on the field. Thus, tolerant cultivars to high salt concentrations in these phases are critical in breeding programs. This way, we aimed to investigate seeds production in conditions of water and salt stress on maize seeds germination and vigor as well as the α-amylase enzyme activity. Two experiments were performed for maize seeds production in salt stress conditions and drought. The experiments were conducted in a randomized complete block design in a split-plot arrangement. Seeds harveste at different development stages, determined by milk line (ML), were submitted to vigor and physiological analysis as well as the expression determination of α-amylase enzyme by electrophoreses. With the results it was concluded that both, the seeds germination and vigor produced in soil with conductivity 3 dS m-1, are not affected by salinity, and the plants development in water and salt stress conditions did not affect the activity of α-amylase enzyme in seedsharvested in ML5 maturity stage. Keywords: Zea mays L., electrophoresis, development stages.

scholarly journals Evaluation of intercropping systems in spring maize with sunflower and urdbean in North Western plain of India

2014 ◽  
Vol 12 (1) ◽  
pp. 26-32 ◽  
Author(s):  
A Bhatnagar ◽  
MS Pal
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Block Design ◽  
Biological Efficiency ◽  
Land Equivalent Ratio ◽  
Randomized Block Design ◽  
Spring Maize ◽  
Sole Cropping ◽  
North Western ◽  
Equivalent Yield

A field experiment was conducted for two years (2007 and 2008) at the G. B. Pant University of Agriculture and Technology, Pantnagar, to study the productivity, biological efficiency and economics of intercropping in spring maize (Zea mays L.) with sunflower (Helianthus annuus L.) and urdbean (Vigna mungo L.) under different spatial arrangements. The experiment consisting of ten treatments i.e. three sole crops (maize, sunflower and urdbean), six replacement intercropping system of maize with each sunflower and urdbean in row ratios of 1:1, 2:1 and 3:1 and one additive intercropping system of maize with urdbean in row ratios of 1+1 was laid out in randomized block design with three replications. The intercropping system of maize with urdbean in row ratio (1+1) was found beneficial over sole cropping of maize and gave the maximum maize-equivalent yield (5847 kg ha-1), land-equivalent ratio (1.36) and net return (Rs.13420 ha-1). Intercropping of maize in replacement arrangement was not productive and advantageous than respective sole crops. DOI: http://dx.doi.org/10.3329/sja.v12i1.21110 SAARC J. Agri., 12(1): 26-32 (2014)

scholarly journals Evaluation of nitrogen (Urea) on Zea mays L. under Balkh province climate

2020 ◽  
Vol 3 (1) ◽  
pp. 6-9
Author(s):  
Nasratullah Habibi ◽  
Friba Sikandari
Keyword(s):  
Zea Mays ◽  
Nitrogen Fertilizer ◽  
Yield Components ◽  
Zea Mays L ◽  
Agricultural Research ◽  
Block Design ◽  
High Yield ◽  
Randomized Complete Block Design ◽  
Yield And Yield Components ◽  
Effect On Yield

An experiment was conducted to evaluate effect of urea fertilizer on yield and yield components of Zea mays L. Using Randomized Complete Block Design (RCBD) split plots in three replications in agricultural research farm of Balkh University by 2019. Doses of urea used in this experiment were 0, 80, 160 and 240 kg ha-1 , respectively. It has been found that amount of nitrogen fertilizer (urea) had significant effect on yield of maize at p<0.05. The higher level of nitrogen caused the higher grain yield, number of kernels per ear, the number of grains per ear row, ear diameter, cob length, grain per plant and plant height. As a result 7.76 ton ha-1 was recorded as high yield while 240 kg ha-1 urea was used, and 5.12 ton ha-1 was recorded as low yield in treatment one with 0 kg ha-1 of nitrogen fertilizer. Finally, as a result using 240 kg ha-1 nitrogen fertilizer is recommended.

scholarly journals Zeamatin-Like Protein (ZLP) Gene is Associated with Resistance against A. niger in Maize (Zea mays L.)

2016 ◽  
Vol 49 (2) ◽  
pp. 29-39
Author(s):  
A.M. Sajjad ◽  
T. Bahsir ◽  
S. Saeed ◽  
M. Iqbal ◽  
S. Islam ◽  
...  
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Hyphal Growth ◽  
Antimicrobial Activities ◽  
Fungal Species ◽  
High Rate ◽  
Sds Page ◽  
Pathogenesis Related ◽  
Enhanced Expression ◽  
Related Proteins

Abstract Maize (Zea mays L.) constitutes one of the most important crops worldwide with multi-billion dollar annual revenue. The plant is however a good substrate for growth, development and activity of filamentous fungi. A large number of fungal species causes spoilage and accumulation of mycotoxins. Plants restrict the hyphal growth by producing pathogenesis related proteins. So far 17 groups of such proteins are identified. PR-5 group comprises of the thaumatin-like proteins (TLPs), which have diverse modes of actions and act at various stages of fungal attack. Zeamatin-like protein (ZLP) is a member of TLPs, which is basically localized in seeds with enhanced expression during physiological growth and cellular differentiation. However a basal quantity is found in the leaves of many crop plants. Here we report the response of maize plant tissues against A. niger inoculation by measuring the variation in expression profile of a zeamatin-like gene. Conventional PCR coupled with RT-qPCR identifies a significant change in the expression magnitude of ZLP in pre- and post-inoculated plant samples. SDS-PAGE, followed by antimicrobial activities against A. niger, E.coli, P. aeruginosa, B. cereus, S. aureus and S. typhimurium, however, do not register a direct relationship with enhancement in gene expression. It is in line with the fact that response to pathogenesis in plants is a multigenic activity involving a series of responsible/induced genes. The assay developed is useful in primary sorting out of the maize hybrids with respect to their resistance against Aspergillus spp., especially in areas with high rate of incidence of fungal pathogenesis.

scholarly journals MÉTODOS DE DETECÇÃO DO GÊNERO Aspergillus EM SEMENTES DE MILHO (Zea mays L.) EM 270 DIAS DE ARMAZENAMENTO

2003 ◽  
Vol 4 (1) ◽  
Author(s):  
G. M. CIRIO ◽  
M. L. R. Z. C. LIMA
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Animal Health ◽  
Potato Dextrose Agar ◽  
Detection Methods ◽  
Secure Storage ◽  
Maize Seeds ◽  
Low Humidity ◽  
The Media ◽  
Seeds Germination

O gênero Aspergillus tem como característica desenvolver-se em sementes e grãos com baixa umidade causando deterioração destes durante armazenamento com efeito na germinação nas sementes e produção de micotoxinas como aflatoxina em grãos, prejudiciais a saúde do homem e dos animais. Para avaliar a eficiência de métodos de detecção para Aspergillus, verificar germinação e umidade das sementes de milho foi feita armazenagem por 270 dias. As avaliações foram ao zero, 90, 150, 210 e 270 dias após armazenamento (DAA) com testes em papel filtro (blotter) e nos meios de batata dextrose ágar ácido (BDA ácido) e ágar suco de tomate mais 6% de cloreto de sódio (AST salino). Como resultados ao zero dia de armazenamento verificou-se ausência de Aspergillus; aos 90 DAA incidências 7,2% em blotter e 1,1% em AST; aos 150 DAA incidências 5,1% em blotter; aos 210 DAA incidências 41,1% em AST, 5,1% no blotter e 3,3% no BDA e aos 270 DAA incidências de 79,7% e 66,2% em blotter e AST que não diferiram estatisticamente entre si e 21% em BDA. A germinação e umidade foram: 98% e 10,8% (dia zero); 93% e 13,4% (90 DAA); 93% e 12,2% (150 DAA); 92% e 14,2% (210 DAA) e 36 % e 12,2 % (270 DAA), respectivamente. Concluiu-se que o meio AST salino mostrou maior eficiência para detectar Aspergillus aos 210 DAA indicando proliferação do gênero durante armazenamento até 270 DAA. A germinação das sementes reduziu-se de 92% para 36% entre 210 e 270 DAA e a umidade variou entre 10,8% e 14,2%, compatível com armazenagem segura. DETECTION METHODS OF Aspergillus GENUS IN CORN SEEDS (Zea mays L.) DURING 270 DAYS STORAGE Abstract The genera Aspergillus has a characteristic to developed in seeds and grains with low humidity causing deterioration during storage with effects on the seeds germination andmicotoxins production as aflatoxin in grains that are harmful to man and animal health. To evaluated the efficiency of detection methods for Aspergillus, to confirm germination and humidity in maize seeds, they were stored for 270 days. The evaluations were at zero, 90, 150, 210 and 270 days after storage (DAS) with the blotter test and in media potato-dextrose-agar (PDA acid) and tomato juice agar plus 6% NaCl (salty TJA). As results at zero day absence of Aspergillus, at 90 DAS incidence 7,2% in blotter and 1,1% in TMA, at 150 DAS incidence 5,1% in blotter; at 210 DAS the incidence 41,1% in TMA, 5,1% in blotter e 3,3% in PDA and at 270 DAS incidence 79,7% and 66,2% in blotter and TMA that didn’t differ statistically and 21% in PDA. Were get germination and humidity 98% and 10,8% (zero day); 93% and 13,4% (90 DAS); 93% and 12,2% (150 DAS); 92% and 14,2% (210 DAS) and 36% and 12,2% (270 DAS) respectively. The conclusions were that the media salty TJA showed higher efficiency to detect Aspergillus at 210 DAS what indicated proliferation of the genera during storage till 270 DAS. The germination reduced from92% to 36% between 210 e 270 DAS and humidity remained between 10,8% and 14,2% compatible with secure storage.

ARRANJO POPULACIONAL PARA A VARIEDADE UENF-14 DE MILHO PIPOCA

2017 ◽  
Vol 16 (3) ◽  
pp. 401
Author(s):  
CÁSSIO VITORAZZI ◽  
ANTONIO TEIXEIRA AMARAL JÚNIOR ◽  
LILIAM SILVIA CANDIDO ◽  
ISMAEL LOURENÇO DE JESUS FREITAS ◽  
THIAGO RODRIGUES DA CONCEIÇÃO SILVA
Keyword(s):  
Zea Mays ◽  
Grain Yield ◽  
Rio De Janeiro ◽  
Zea Mays L ◽  
Block Design ◽  
Grain Production ◽  
Maize Population ◽  
The North ◽  
Randomized Complete Block Design ◽  
Janeiro State

 RESUMO - A adequação do arranjo populacional está entre as técnicas que podem maximizar a produtividade da cultura do milho. Daí a importância das pesquisas que buscam estudar o desempenho de cultivares de milho e milhos especiais, a fim de determinar o arranjo de plantas ideal. O objetivo deste trabalho foi avaliar o desempenho da variedade UENF-14, de milho pipoca, cultivada em diferentes arranjos populacionais, na safra e safrinha, para otimizar a recomendação fitotécnica para os produtores rurais do Norte Fluminense. Foram instalados dois experimentos em Campos dos Goytacazes-RJ. Um na safrinha 2011 utilizando a variedade UENF-14 e o híbrido experimental P2 x P9, e o outro na safra 2011-2012, com a variedade UENF-14 e o híbrido comercial IAC-112. O delineamento experimental, nos dois experimentos, foi o de blocos casualizados arranjados em parcelas subsubdivididas, em que as parcelas foram compostas pelos genótipos, as subparcelas constituídas pelos espaçamentos entre linhas de semeadura (0,45, 0,60 e 0,90 m) e as subsubparcelas compostas pelas densidades de plantas (60.000, 75.000 e 90.000 plantas ha-1). A UENF-14 apresentou desempenho superior aos híbridos, demonstrando seu potencial para cultivo na região Norte Fluminense. Houve aumento do rendimento de grãos com a diminuição do espaçamento entre linhas de plantio. Para a variedade UENF-14, a redução do espaçamento entre linhas de plantio foi vantajosa para a produção de grãos, independentemente da época de semeadura.Palavras-chave: Zea mays L., densidade de semeadura, espaçamento entre linhas, épocas de semeadura.  POPULATION ARRANGEMENT FOR THE VARIETY OF POPCORN UENF-14 ABSTRACT - The adequacy of the maize population arrangement is among the most important techniques for obtaining higher yields. Therefore, many researchers have been conducting an attempt to study the performance of corn and specialty corn. The objective was to evaluate the performance of the popcorn variety UENF-14 in different population arrangements, in the harvest season and off-season. Two experiments were carried out in Campos dos Goytacazes, North of Rio de Janeiro State, Brazil, in the off-season 2011, using the UENF-14 variety and the experimental hybrid P2 x P9, and in the season 2011-2012 using UENF-14 and the commercial hybrid IAC-112. The treatments were arranged in randomized complete block design with split plots, in which plots were composed by popcorn genotypes, the subplots consisting of three spacings between sowing lines (0.45, 0.60 and 0.90 m) and the subsubplots composed of three plants per hectare densities (60,000, 75,000 and 90,000 plants.ha-1). The variety UENF-14 presented higher performance than the hybrids, demonstrating its potential for recommendation to the North of Rio de Janeiro State. There was an increase of grain yield by reducing the spacing between the plants rows. The cultivation of the variety UENF-14 reducing the spacing between rows was advantageous for grain production, regardless of planting date.Keywords: Zea mays L., densities of plants, spacing between rows, sowing times.

PERFORMANCE PRODUTIVA DE CULTIVARES DE MILHO EM TRÊS SISTEMAS DE CULTIVO NO LESTE MARANHENSE

2017 ◽  
Vol 15 (3) ◽  
pp. 520
Author(s):  
FRANCISCO CHARLES DOS SANTOS SILVA ◽  
MARIA DA CRUZ CHAVES LIMA MOURA ◽  
JOSÉ RONEILSON SILVA COSTA ◽  
EGON BASTOS DE OLIVEIRA ◽  
ITALO JHONNY COSTA
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Block Design ◽  
Cropping System ◽  
Farming Systems ◽  
Correct Choice ◽  
Local Conditions ◽  
Maize Cultivars ◽  
Randomized Block Design ◽  
The City

RESUMO – O Leste Maranhense corresponde à mais nova fronteira agrícola do Brasil. Entretanto, fatores limitantesda produtividade, como a escolha correta da variedade e o sistema de cultivo mais adequado para as condições locais,ainda são desconhecidos. Objetivou-se estudar o desempenho produtivo de 17 genótipos de milho, sob três sistemasde cultivo para a região. Foram realizados dois experimentos, um na cidade de Chapadinha e outro na cidade de Brejo,sendo avaliados 17 genótipos ao todo. Os ensaios foram dispostos no delineamento em blocos casualisados com quatrorepetições em esquema fatorial 3x10 [10 cultivares e três sistemas de cultivo (plantio com cobertura de palha decarnaúba; plantio sem cobertura; plantio intercalado com feijão caupi)]. O sistema de cultivo com palha de carnaúba,independentemente do genótipo de milho e do local de avaliação, proporcionou os maiores rendimentos de grãos.Em Chapadinha, os genótipos mais produtivos foram as variedades de polinização aberta Caiano e Al-Bandeirante,o híbrido duplo AGN 1051, os híbridos triplos 2B 433, BRS 3060 e CMS 3E482 e o híbrido simples P 3646 H. ParaBrejo, os melhores resultados foram obtidos pelos híbridos duplos 2B 707 HX e AGN 1051 e pelos híbridos simples2B 604 HX, 30 F35 H e P 3646 H.Palavras-chave: Zea mays L., Palhada, Rendimento de grãos.PRODUCTIVE PERFORMANCE OF MAIZE CULTIVARS IN THREE FARMING SYSTEMS IN EAST MARANHÃO STATEABSTRACT - East Maranhão State is the newest agricultural frontier in Brazil. However, productivity limiting factorssuch as the correct choice of variety and the cropping system more suitable to the local conditions are still unknown.The objective of this study was to evaluate the productive performance of 17 maize genotypes under three cultivationsystems for the region. Two experiments were carried out, one in the city of Chapadinha and another in the city ofBrejo, evaluating 17 genotypes. The trials were arranged in a randomized block design with 4 replications in a 3x10factorial scheme [10 cultivars and 3 cultivation systems (planting with cover of carnaúba straw, plantation withoutcover, intercropping with cowpea). The use of carnaúba straw provided the highest grain yields, regardless the maizegenotype and the evaluation site,. In Chapadinha the most productive genotypes were the open-pollinated varietiesCaiano and Al-Bandeirante, the double hybrid AGN 1051, the triple hybrids 2B 433, BRS 3060 and CMS 3E482 andthe simple hybrid P 3646 H. For Brejo the best results were obtained by the double hybrids 2B 707 HX and AGN 1051and by the simple hybrids 2B 604 HX, 30 F35 H and P 3646 H.Keywords: Zea mays L., Straw, Grain Yield.

EFICIÊNCIA DE BIOESTIMULANTES E FUNGICIDA NOS CARACTERES AGRONÔMICOS E ECONÔMICOS NA CULTURA DO MILHO VERDE

2018 ◽  
Vol 17 (2) ◽  
pp. 274
Author(s):  
RICARDO FRANCISCHINI ◽  
ALESSANDRO GUERRA DA SILVA ◽  
DAURI JOSÉ TESSMANN
Keyword(s):  
Zea Mays ◽  
Chlorophyll Content ◽  
Plant Height ◽  
Zea Mays L ◽  
Block Design ◽  
Stem Diameter ◽  
Production Costs ◽  
Net Income ◽  
Randomized Block Design ◽  
Presence And Absence

 RESUMO - O objetivo do trabalho foi avaliar o desempenho agronômico e econômico na cultura do milho verde submetida ao uso de bioestimulantes na presença e ausência de fungicida. Dessa forma, dois experimentos foram conduzidos em anos distintos, em delineamento experimental de blocos ao acaso com três repetições, em arranjo fatorial 4x2. O primeiro fator correspondeu à combinação de bioestimulantes (Ausência, MC Cream, MC Extra, MC Cream+MC Extra) e o segundo fator à presença e ausência de fungicida (piraclostrobina + epoxiconazole). Foi avaliado o peso de espigas verdes, a altura de plantas, diâmetro de colmo, teor de clorofila e severidade de doenças, além da viabilidade econômica dos tratamentos. Os Bioestimulantes, na ausência de fungicida, foram eficientes para aumentar o peso das espigas verdes, altura de plantas, e o teor de clorofila. Na presença do fungicida os bioestimulantes aumentaram o diâmetro de colmo e reduziram a severidade de doenças. Os investimentos em insumos elevaram os custos de produção, porém a rentabilidade foi compensada pelo aumento da renda bruta. Em situação de baixa disponibilidade hídrica, o bioestimulante MC Extra foi o mais viável economicamente para a cultura do milho verde.Palavras-chave: fitossanidade, produtividade, renda líquida, Zea mays L. EFFICIENCY OF BIOSTIMULANTS AND FUNGICIDE IN THE AGRONOMIC AND ECONOMIC CHARACTERS OF GREEN CORN  ABSTRACT – The objective of this work was to evaluate the agronomic and economic performance of the green corn crop, subjected to the use of biostimulants in the presence and absence of fungicide. Two experiments were conducted in different years using a randomized block design with three replications, in a 4x2 factorial arrangement. The first factor corresponded to the combination of biostimulants (Absence, MC Cream, MC Extra, MC Cream + MC Extra) and the second factor was the presence and absence of fungicide (pyraclostrobin + epoxiconazole). Green ear weight, plant height, stem diameter, chlorophyll content and severity of diseases were evaluated, as well as the economic viability of the treatments.In the absence of fungicide, biostimulants were efficient to increase the green ear weight, plant height, and chlorophyll content. In the presence of the fungicide, biostimulants increased stem diameter and reduced disease severity. Investments in inputs increased production costs, but profitability was offset by the increase in gross income. In a situation of low water availability, the biostimulant MC Extra was the most economically viable for the green maize crop.Keywords: plant health, productivity, net income, Zea mays L.

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