scholarly journals Qici Sanling Decoction Suppresses Glutamine Consumption and Bladder Cancer Cell Growth through Inhibiting c-Myc Expression

2022 ◽  
Vol 2022 ◽  
pp. 1-9
Author(s):  
Weihua Chen ◽  
Weifeng Wang ◽  
Jun Zhang ◽  
Guoqiang Liao ◽  
Jie Bai ◽  
...  

Traditional Chinese medicine (TCM) is widely used as an alternative therapy for cancer treatment in China. Glutamine catabolism plays an important role in cancer development. Qici Sanling decoction (QCSL) suppresses bladder cancer growth. However, the association between QCSL and glutamine catabolism remains unknown. In this study, different doses of QCSL were applied to T24 cells, followed by the measurements of cell viability and apoptosis using CCK-8 and Annexin V/PI assay, respectively. Furthermore, glutamine consumption was detected using the glutamine assay kit. QCSL was observed to inhibit cell growth and induced cell apoptosis in a dose-dependent manner. Analysis of glutamine consumption revealed that QCSL suppressed glutamine consumption in T24 cells. Furthermore, QCSL decreased the mRNA and protein levels of c-Myc, GLS1, and SLC1A5. All these effects induced by QCSL could be alleviated by c-Myc overexpression, indicating c-Myc was involved in the protective role of QCSL in bladder cancer. In addition, QCSL was found to inhibit tumor growth in the xenograft tumor model. The similar results were obtained in tumor samples that protein levels of c-Myc, GLS1, and SLC1A5 were decreased upon treatment with QCSL. In conclusion, QCSL suppresses glutamine consumption and bladder cancer cell growth through inhibiting c-Myc expression.

2020 ◽  
Vol 19 (2) ◽  
pp. 253-257
Author(s):  
Junwei Xu ◽  
Ning Qin ◽  
Yebin Yao ◽  
Tao Chen ◽  
Wenbo Jiang

Purpose: The effect of geraniin on human bladder transitional carcinoma was not clear, this study was thus intended to reveal it and reveal the mechanism. Methods: T24 cells were treated with 5, 10, and 20 μM of geraniin and the viability and apoptosis of T24 cells were determined using thiazolyl blue tetrazolium bromide (MTT) assay and flow cytometry. The protein expression levels of Cyclin D1, p21, BAL-2, BAX, cleaved caspase-3 and PI3K/AKT pathway were evaluated using western blot. Results: Geraniin decreased T24 cell viability and induced T24 cell cycle arrest. The proportion of T24 cells in S phase was decreased by geraniin. Besides, geraniin promoted T24 cell apoptosis and regulated PI3K/AKT pathway. Conclusion: Geraniin appears to regulate bladder cancer cell growth by decreasing the levels of PI3K and AKT phosphorylation. Thus, this agent may be useful in the management of bladder cancer Keywords: Geraniin, T24 cells, Apoptosis, PI3K/AKT signaling


2013 ◽  
Vol 14 (6) ◽  
pp. 12297-12312 ◽  
Author(s):  
Doreen Kunze ◽  
Kati Erdmann ◽  
Michael Froehner ◽  
Manfred Wirth ◽  
Susanne Fuessel

2019 ◽  
Vol 27 (5) ◽  
pp. 1028-1038
Author(s):  
Qipeng Xie ◽  
Caiyi Chen ◽  
Haiying Li ◽  
Jiheng Xu ◽  
Lei Wu ◽  
...  

2012 ◽  
Vol 30 (6) ◽  
pp. 920-927 ◽  
Author(s):  
Hu Zhang ◽  
Haowen Jiang ◽  
Wei Wang ◽  
Jian Gong ◽  
Limin Zhang ◽  
...  

2016 ◽  
Vol 195 (4S) ◽  
Author(s):  
Takashi Kawahara ◽  
Hiroki Ide ◽  
Hasanain Khaleel Shareef ◽  
Satoshi Inoue ◽  
Eiji Kashiwagi ◽  
...  

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