Metabolic alterations and WNT signaling impact immune response in HGSOC

Microglia play an integral role in brain development but are also crucial for repair and recovery after traumatic brain injury (TBI). TBI induces an intense innate immune response in the immature, developing brain that is associated with acute and chronic changes in microglial function. These changes contribute to long-lasting consequences on development, neurologic function, and behavior. Although alterations in glucose metabolism are well-described after TBI, the bulk of the data is focused on metabolic alterations in astrocytes and neurons. To date, the interplay between alterations in intracellular metabolic pathways in microglia and the innate immune response in the brain following an injury is not well-studied. In this review, we broadly discuss the microglial responses after TBI. In addition, we highlight reported metabolic alterations in microglia and macrophages, and provide perspective on how changes in glucose, fatty acid, and amino acid metabolism can influence and modulate the microglial phenotype and response to injury.

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Wnt Signaling Regulates Macrophage Mediated Immune Response to Pathogens

Macrophage Activation - Biology and Disease ◽

10.5772/intechopen.86433 ◽

2020 ◽

Author(s):

Suborno Jati ◽

Malini Sen

Keyword(s):

Immune Response ◽

Wnt Signaling

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Evaluation of the Effect of Probiotic Administration on Gene Expression in Goat Blood

Journal of Molecular Biology Research ◽

10.5539/jmbr.v7n1p88 ◽

2017 ◽

Vol 7 (1) ◽

pp. 88 ◽

Cited By ~ 6

Author(s):

Kingsley Ekwemalor ◽

Emmanuel Asiamah ◽

Bertha Osei ◽

Hamid Ismail ◽

Mulumebet Worku

Keyword(s):

Immune Response ◽

Wnt Signaling ◽

Signaling Pathway ◽

Wnt Signaling Pathway ◽

Adaptive Immune Response ◽

Control Group ◽

Sterile Water ◽

Adaptive Immune ◽

Expression Of Genes ◽

Probiotic Treatment

The objective of this study was to assess the molecular impact of probiotic administration on genes involved in homeostasis and immunity in goat blood. Following initial screening for infection, one-week post weaning, female SpanishXBoer goats were drenched daily with the recommended doses of FASTtrak microbial pack (Conklin Company Inc., Shakopee, MN) in 10 mL sterile water over an 8-week period. The control group were given sterile water. Blood samples were collected weekly. Total RNA was isolated from blood collected at the beginning of the study (week 0) and at the end of the study (week 8) using Tri-reagent and then reverse-transcribed to cDNA using the Ambion-Retroscript kit. Quantification of genes was performed in the CFX96TM Biorad Real-Time PCR detection system with the addition of the dye SYBR green. The cow Wingless (Wnt) signaling pathway, Human Innate & Adaptive Immune Responses and the Cow Inflammatory Cytokine & Receptors RT² Profiler™ PCR Arrays (QIAGEN, Valencia, CA) were used to profile the expression of 84 genes involved in each pathway. Probiotic treatment had no effect on body weight, body condition, fecal egg count and RNA concentration (p>0.05). Packed cell volume and FAMACHA scores were significantly improved by probiotic administration. Results from RT-PCR showed increased expression of genes in innate and adaptive immune response, cytokine and Wnt pathways in response to probiotics. Probiotics induced the expression of 32 genes involved in innate and adaptive immune response inflammatory cytokines, and 48 genes involved in the Wnt signaling pathway. This study provides evidence for a systematic effect of oral probiotic administration on expression of genes involved in immunity and homeostasis in goat blood.

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Wnt Signaling Plays a Key Role in the Regulation of the Immune Response and Cardiac Damage during Trypanosoma cruzi Infection

ACS Infectious Diseases ◽

10.1021/acsinfecdis.0c00590 ◽

2021 ◽

Vol 7 (3) ◽

pp. 566-578

Author(s):

Ximena Volpini ◽

Laura Fernanda Ambrosio ◽

María Agustina Brajín ◽

María Belen Brugo ◽

María Pilar Aoki ◽

...

Keyword(s):

Immune Response ◽

Trypanosoma Cruzi ◽

Wnt Signaling ◽

Cardiac Damage ◽

Cruzi Infection

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Evaluation of the systemic innate immune response and metabolic alterations of nonlactating cows with diet-induced subacute ruminal acidosis

Journal of Dairy Science ◽

10.3168/jds.2014-8319 ◽

2014 ◽

Vol 97 (12) ◽

pp. 7777-7787 ◽

Cited By ~ 17

Author(s):

J.C. Rodríguez-Lecompte ◽

A.D. Kroeker ◽

A. Ceballos-Márquez ◽

S. Li ◽

J.C. Plaizier ◽

...

Keyword(s):

Immune Response ◽

Innate Immune Response ◽

Innate Immune ◽

Subacute Ruminal Acidosis ◽

Metabolic Alterations ◽

Ruminal Acidosis

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Kynurenine and Hemoglobin as Sex-Specific Variables in COVID-19 Patients: A Machine Learning and Genetic Algorithms Approach

Diagnostics ◽

10.3390/diagnostics11122197 ◽

2021 ◽

Vol 11 (12) ◽

pp. 2197

Author(s):

Jose M. Celaya-Padilla ◽

Karen E. Villagrana-Bañuelos ◽

Juan José Oropeza-Valdez ◽

Joel Monárrez-Espino ◽

Julio E. Castañeda-Delgado ◽

...

Keyword(s):

Machine Learning ◽

Genetic Algorithm ◽

Immune Response ◽

Support Vector Machine ◽

Clinical Manifestations ◽

Disease Diagnosis ◽

Machine Learning Algorithms ◽

Support Vector ◽

Men And Women ◽

Metabolic Alterations

Differences in clinical manifestations, immune response, metabolic alterations, and outcomes (including disease severity and mortality) between men and women with COVID-19 have been reported since the pandemic outbreak, making it necessary to implement sex-specific biomarkers for disease diagnosis and treatment. This study aimed to identify sex-associated differences in COVID-19 patients by means of a genetic algorithm (GALGO) and machine learning, employing support vector machine (SVM) and logistic regression (LR) for the data analysis. Both algorithms identified kynurenine and hemoglobin as the most important variables to distinguish between men and women with COVID-19. LR and SVM identified C10:1, cough, and lysoPC a 14:0 to discriminate between men with COVID-19 from men without, with LR being the best model. In the case of women with COVID-19 vs. women without, SVM had a higher performance, and both models identified a higher number of variables, including 10:2, lysoPC a C26:0, lysoPC a C28:0, alpha-ketoglutaric acid, lactic acid, cough, fever, anosmia, and dysgeusia. Our results demonstrate that differences in sexes have implications in the diagnosis and outcome of the disease. Further, genetic and machine learning algorithms are useful tools to predict sex-associated differences in COVID-19.

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The Wnt signaling pathway in tumorigenesis, pharmacological targets, and drug development for cancer therapy

Biomarker Research ◽

10.1186/s40364-021-00323-7 ◽

2021 ◽

Vol 9 (1) ◽

Author(s):

Zhuo Wang ◽

Tingting Zhao ◽

Shihui Zhang ◽

Junkai Wang ◽

Yunyun Chen ◽

...

Keyword(s):

Immune Response ◽

Tumor Microenvironment ◽

Wnt Signaling ◽

Signaling Pathway ◽

Cancer Progression ◽

Target Genes ◽

Wnt Signaling Pathway ◽

Systematic Evaluation ◽

Beta Catenin ◽

Downstream Target

AbstractWnt signaling was initially recognized to be vital for tissue development and homeostasis maintenance. Further studies revealed that this pathway is also important for tumorigenesis and progression. Abnormal expression of signaling components through gene mutation or epigenetic regulation is closely associated with tumor progression and poor prognosis in several tissues. Additionally, Wnt signaling also influences the tumor microenvironment and immune response. Some strategies and drugs have been proposed to target this pathway, such as blocking receptors/ligands, targeting intracellular molecules, beta-catenin/TCF4 complex and its downstream target genes, or tumor microenvironment and immune response. Here we discuss the roles of these components in Wnt signaling pathway in tumorigenesis and cancer progression, the underlying mechanisms that is responsible for the activation of Wnt signaling, and a series of drugs targeting the Wnt pathway provide multiple therapeutic values. Although some of these drugs exhibit exciting anti-cancer effect, clinical trials and systematic evaluation should be strictly performed along with multiple-omics technology.

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