Status of Vitamin B12 and Folate among the Urban Adult Population in South India

2015 ◽  
Vol 68 (2) ◽  
pp. 94-102 ◽  
Author(s):  
M. Sivaprasad ◽  
T. Shalini ◽  
N. Balakrishna ◽  
M. Sudarshan ◽  
P. Lopamudra ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Vitamin B12 ◽  
Wide Spectrum ◽  
Adult Population ◽  
Plasma Concentrations ◽  
Age Groups ◽  
Healthy Adults ◽  
Significant Difference ◽  
B12 Deficiency ◽  
Apparently Healthy

Background: Deficiency of vitamin B12 (B12) and folate (FA) leads to a wide spectrum of disorders that affect all age groups. However, reports on B12 and FA status in healthy adults in India are limited. Hence, we determined the plasma levels and dietary intake of B12 and FA in the adult population. Methods: We conducted a community-based cross-sectional study in an urban setup among 630 apparently healthy adults distributed into 3 age groups: 21-40, 41-60 and >60 years. Plasma concentrations of B12 and FA were analyzed by radio immunoassay and dietary intake by 24-hour recall method. Results: The overall prevalence of FA deficiency was 12%, but there was no significant difference in plasma FA concentrations among the groups. While the overall prevalence of B12 deficiency was 35%, it was significantly higher in the 21-40 (44%) and 41-60 age groups (40%) when compared with the >60 group (30%). B12 deficiency was higher in vegetarians (54%) compared to those consuming mixed diet (31%), and the reverse was the case with FA. However, the dietary intakes of FA and B12 were not significantly different among the groups. Conclusions: These results indicate a higher prevalence of B12 deficiency in apparently healthy adults in an urban setup.

scholarly journals ULTRASOUND;

2012 ◽  
Vol 19 (02) ◽  
pp. 187-192
Author(s):  
EJAZ HUSSAIN SIDDIQUI ◽  
SAAD SIDDIQUI ◽  
NOREEN SHAH
Keyword(s):  
Pediatric Population ◽  
Adult Population ◽  
Age Groups ◽  
X Rays ◽  
Geriatric Population ◽  
Cross Sectional ◽  
Study Results ◽  
Significant Difference ◽  
Ultrasound Findings ◽  
Hepatobiliary Diseases

Objectives: To assess common presenting features and the role of Ultrasound in evaluation of Hepatobiliary diseases in ourpopulation. Design: Cross sectional study. Setting: Khyber X rays, Khyber Medical Centre, Peshawar. Period: August 2010 to December2010. Material and Methods: Data from patients presenting for evaluation of Hepatobiliary diseases was analyzed for presenting complaintsand ultrasound findings according to objectives of the study. Results: In total 197 cases were included in the study. The age wise categorizationincluded pediatric population (6.1%) adult population (87.3%) and geriatric population (6.6%). Upper abdominal pain was the most commonpresenting feature in all age groups as well as in both genders. Cholelithiasis with or without cholecystitis was the most common ultrasoundfinding in all age groups. No statistically significant difference was found between presenting features or ultrasound findings in different agegroups as well as across gender. Conclusions: Hepatobiliary diseases are among major illnesses in our region. Ultrasound is a sensitive anduseful tool for screening and evaluation of Hepatobiliary disorders.

scholarly journals Role of Functional Biomarkers to Identify Early Vitamin B12 Deficiency in Patients with Sleeve Gastrectomy: A Cross-Sectional Study

Medicina ◽  
2020 ◽  
Vol 56 (3) ◽  
pp. 142
Author(s):  
Osama Y. Al-Dirbashi ◽  
Charu Sharma ◽  
Nahid Al Dahouri ◽  
Anas Al Aidaros ◽  
Shamma Al-Muhairi ◽  
...  
Keyword(s):  
Sleeve Gastrectomy ◽  
Vitamin B12 ◽  
Cross Sectional Study ◽  
Control Group ◽  
Sectional Study ◽  
Cross Sectional ◽  
Significant Difference ◽  
B12 Deficiency ◽  
One Year ◽  
Functional Biomarkers

Background and objectives: Although laparoscopic sleeve gastrectomy (LSG) is effective for obesity management, postoperative vitamin B12 (B12) deficiency is of major concern. In this cross-sectional study, we assessed the levels of B12 and its related functional biomarkers, namely, total homocysteine (tHcy), methylmalonic acid (MMA), folate, methylcitric acid (MCA), and hemoglobin (Hb), in one-year postoperative LSG patients and matched controls. Materials and Methods: Plasma B12, tHcy, MMA, folate, and MCA were measured in matched controls (n = 66) and patients (n = 71) using validated liquid chromatography-tandem mass spectrometry techniques and protocols in the United Arab Emirates (UAE). Results: The median B12 concentration in patients (177 pmol/L) was significantly lower (p < 0.001) than in the controls (334.7 pmol/L). The tHcy and MMA levels were significantly increased (p < 0.001 and p = 0.011, respectively) and folate levels were significantly decreased (p = 0.001) in the LSG patients compared to the controls. Interestingly, no significant difference in MCA levels were observed between the two groups. The levels of tHcy and MMA were concomitantly increased with the decreased folate levels in postoperative LSG patients when compared with the controls. The Hb levels were significantly lower in males and females in the patient group compared with those in the control group, respectively (p = 0.005 and p = 0.043). Conclusions: This is the first report of serum levels of B12 and its functional biomarkers in postoperative LSG patients among a local population from the UAE. Our findings revealed significant alterations of the B12 biomarkers, total B12, MMA, and tHcy in one-year postoperative LSG patients.

scholarly journals Vitamin B12 Absorption as Measured by Total B12 and Holo-TC Following A Single Oral Dose of Multivitamin in Healthy Adults

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1332-1332
Author(s):  
Kelly Zhang ◽  
William Hooper ◽  
Laura Harkness ◽  
Annahita Ghassemi
Keyword(s):  
Oral Dose ◽  
Vitamin B12 ◽  
Single Oral Dose ◽  
Phase 1 ◽  
Healthy Adults ◽  
Serum Samples ◽  
Funding Sources ◽  
Significant Difference ◽  
Vitamin B12 Absorption ◽  
The Difference

Abstract Objectives Measure total B12 and holotranscobalamin (Holo-TC) concentrations in serum to show vitamin B12 absorption following single oral doses of multivitamin (MVI) gummy and tablet. Methods This crossover clinical trial involved healthy adults randomized to either gummy or tablet multivitamins (MVI) containing vitamin B12 (60.8 mcg in gummy and 91.8 mcg in tablet) as cyanocobalamin in a single dose in Phase 1 with serial blood samples collected at pre-dose and at 0.5-, 1-, 2-, 4-, 6-, 8-, 9-, 10-, 24-, and 48-hrs after dosing, followed by a 2-week washout period. In Phase 2, participants then crossed over to receive MVI in the form not previously given, with blood draws at the same timepoints. Serum samples were measured for total B12 (n = 19) using an electrochemiluminescence immunoassay (ECLIA) and for Holo-TC (n = 14) using commercial Holo-TC ELISA kit. Measurements from groups of time points were averaged to estimate pre-absorptive (0 to 2 hours) and absorptive (6 to 10 hours) concentrations of total B12 and Holo-TC for each subject. The difference between pre-absorptive and absorptive concentrations for both analytes was calculated to estimate newly absorbed vitamin B12. Results Mean increases of about 15% in serum Holo-TC in absorptive phase over the pre-absorptive phase were observed for both the gummies and the tablets, which are statistically significant (p &lt; 0.001). These changes were similar to those observed for total B12. There was no significant difference in mean increases between the gummies and the tablets in both total B12 and Holo-TC. These results support the conclusion that the observed increases in total B12 and Holo-TC were the result of intestinal absorption following the single oral dose. Conclusions Overall, under the conditions of this study, both gummy and tablet multivitamins showed similar absorption of vitamin B12, whether assessed by total B12 or holo-TC measurement. Funding Sources Church & Dwight Co. Inc.

scholarly journals Dietary Intake and Status of Vitamin B12 in Slovenian Population

Nutrients ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 334
Author(s):  
Živa Lavriša ◽  
Hristo Hristov ◽  
Maša Hribar ◽  
Katja Žmitek ◽  
Anita Kušar ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Age Groups ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
High Serum ◽  
Health Concern ◽  
Dietary Vitamin ◽  
Serum Homocysteine ◽  
Daily Vitamin ◽  
B12 Deficiency

Vitamin B12 deficiency poses a health concern, especially in vulnerable populations. Dietary vitamin B12 intake was obtained by two 24 h dietary recalls and food propensity questionnaires in a representative Slovenian cross-sectional food consumption survey, SI.Menu (n = 1248 subjects; 10–74 years). For a subgroup of 280 participants, data on serum vitamin B12 were available through the Nutrihealth study. The estimated usual population-weighted mean daily vitamin B12 intakes were 6.2 µg (adults), 5.4. µg (adolescents), and 5.0 µg (elderly). Lower intakes were observed in females. Inadequate daily vitamin B12 intake (<4 µg) was detected in 37.3% of adolescents, 31.7% of adults, and 58.3% elderlies. The significant predictors for inadequate daily vitamin B12 intake were physical activity score in all age groups, sex in adolescents and adults, financial status and smoking in elderly, and employment in adults. Meat (products), followed by milk (products), made the highest vitamin B12 contribution in all age groups. In adolescents, another important vitamin B12 contributor was cereals. The mean population-weighted serum vitamin B12 levels were 322.1 pmol/L (adults) and 287.3 pmol/L (elderly). Low serum vitamin B12 concentration (<148 nmol/L) and high serum homocysteine (>15 µmol/L) were used as criteria for vitamin B12 deficiency. The highest deficiency prevalence was found in elderlies (7.0%), particularly in males (7.9%). Factors associated with high serum homocysteine were also investigated. In conclusion, although vitamin B12 status was generally not critical, additional attention should be focused particularly to the elderly.

scholarly journals The profile of hematinic deficiencies in patients with oral lichen planus: a case-control study

2020 ◽  
Author(s):  
Zhe-xuan Bao ◽  
Xiao-wen Yang ◽  
Jing Shi ◽  
Yufeng Wang
Keyword(s):  
Vitamin B12 ◽  
Lichen Planus ◽  
Serum Ferritin ◽  
Oral Lichen Planus ◽  
Vitamin B12 Deficiency ◽  
Serum Folate ◽  
Healthy Controls ◽  
Significant Difference ◽  
B12 Deficiency ◽  
Oral Lichen

Abstract Background Oral lichen planus (OLP) is a relatively common mucocutaneous disorder, its causative factors and pathogenesis still remain obscure. The existing studies on the association between hematinic deficiencies and OLP are limited and inconsistent. The aim of this study was to assess the hematinic deficiencies in a cohort of OLP patients and evaluate the correlation between hematinic deficiencies and OLP. Methods 236 OLP patients and 226 age-and gender-matched healthy controls were enrolled in this study. The levels of hemoglobin (Hb), serum folate, vitamin B12 and ferritin were measured and compared between OLP patients and healthy controls. REU (reticular/hyperkeratotic, erosive/erythematous, ulcerative) scoring system was adopted and compared between the OLP patients with and without hematinic deficiencies. The correlation between hematinic deficiencies and OLP was analyzed. Results The frequencies of serum ferritin and vitamin B12 deficiency in OLP patients were both significantly higher than those of the healthy controls. According to gender and age, the profiles of hematinic deficiencies in OLP patients were significantly different. As for REU score, no significant difference existed between OLP patients with and without hematinic deficiencies. Both serum ferritin deficiency and serum vitamin B12 deficiency were significantly correlated with OLP. Conclusions The present study suggested a significant association between hematinic deficiencies and OLP. Iron, folate, and vitamin B12 levels in OLP patients should be monitored routinely. In the case of hematinic deficiencies, appropriate supplementation therapy is necessary. Further studies are warranted to explore the interactions between OLP and hematinic deficiencies.

Is Age Related Fibrinogen Affected by Warfarin?.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4117-4117
Author(s):  
Roger C. Munro ◽  
Lisa J. Wakeman ◽  
Saad Al-Ismail
Keyword(s):  
Statistical Significance ◽  
Plasma Concentrations ◽  
Age Groups ◽  
Warfarin Group ◽  
Published Evidence ◽  
Age Related ◽  
Age Gap ◽  
Significant Difference ◽  
Warfarin Treatment ◽  
The Difference

Abstract Introduction: There is published evidence which indicates that advancing age may be associated with higher plasma concentrations of fibrinogen. There is also evidence that derived fibrinogen values are significantly higher than Clauss measurements and that this discrepancy is greater in patients receiving warfarin. The purpose of this study was to determine whether age related derived fibrinogen levels are similar in both warfarin and non-warfarin groups. Methods: Venous samples were collected into siliconised glass B-D Vacutainers containing tri-sodium citrate (Ref: 367691) from 1000 patients receiving long term warfarin treatment and an equal number of age-matched patients not receiving warfarin. Genders were equally represented in both groups. Patients in both groups were categorized into 5 years age bands as follows: &lt;40 n=23: 40–44 n=20: 45–49 n=43; 50–54 n=74: 55–59 n=113: 60–64 n=155: 65–69 n=178: 70–74 n=191: 75–79 n=124; 80–84 n=56: 85–89 n=23. Derived fibrinogen was measured in each patient on an ACL300R coagulometer (I L) within 1 hour of collection using IL PT-FIB HS Plus reagent and following the manufacturer’s protocol. Appropriate CLSI guidelines were followed throughout. A normal probability plot of the data was performed to confirm that it did not deviate too much from the normal distribution. Results: The T-test for independent samples using the separate variance estimate showed that there was a statistically significant difference in the mean fibrinogen between patients on warfarin and those not on warfarin (p&lt;0.05) in each group except for the last (85–89 years). There was a statistically significant difference (ANOVA) in the fibrinogen levels of patients of different age in both warfarinised and non-warfarinised groups (p&lt;0.05). The modified least significance procedure in the ANOVA test showed that in the non-warfarin group, most of significant difference in fibrinogen between the different age groups is contributed by the difference between patients under 50 years of age. In the non-warfarin group, it requires an age gap of at least 20 years for the difference in fibrinogen to be statistically significant but in the warfarin group, it only requires an age gap of ten years (p&lt;0.05). Both Linear Regression and Cross Tabulation indicate that the relationship between fibrinogen and age does not vary whether or not the patient is on warfarin. These also show that the effect of age on fibrinogen is not affected by warfarin treatment. Conclusion: Differences or correlations detected in this analysis are of statistical significance but not necessarily clinically significant. Placing age and warfarin treatment in the same model shows that variations in fibrinogen have to be explained by other factors (e.g. technical) not included in the study as only 12% of the error in predicting fibrinogen levels can be reduced by knowing both the age and status of warfarin treatment in individual patients.

Functional vitamin B12 deficiency in phenylketonuria patients and healthy controls: An evaluation with combined indicator of vitamin B12 status as a biochemical index

2020 ◽  
Vol 57 (4) ◽  
pp. 291-299
Author(s):  
Merve Akış ◽  
Melis Kant ◽  
İshak Işık ◽  
Pelin Teke Kısa ◽  
Engin Köse ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Vitamin B12 ◽  
Children And Adolescents ◽  
Methylmalonic Acid ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
Healthy Controls ◽  
Biochemical Index ◽  
Significant Difference ◽  
B12 Deficiency

Background Vitamin B12 deficiency frequently appears in phenylketonuria patients having a diet poor in natural protein. The aims of this study were to evaluate vitamin B12 status in phenylketonuria patients by using combined indicator of vitamin B12 status (cB12) as well as methylmalonic acid and homocysteine, more specific and sensitive markers, in comparison with healthy controls. Methods Fifty-three children and adolescents with phenylketonuria under dietary treatment and 30 healthy controls were assessed cross-sectionally. Serum vitamin B12 and folate concentrations were analysed by chemiluminescence immunoassay. Plasma methylmalonic acid and total homocysteine concentrations were measured by liquid chromatography-tandem mass spectrometry and liquid chromatography, respectively. cB12 was calculated by using a formula involving blood parameters. Results Methylmalonic acid and folate concentrations in phenylketonuria group were higher compared with controls. Methylmalonic acid concentrations were high in 56.5% of the patients and 26.7% of the controls with normal vitamin B12 concentrations. Based on cB12, a significant difference within the normal values was detected between the groups. However, although 24.5% of phenylketonuria patients and 13.3% of controls had decreased vitamin B12 status according to cB12, there was no significant difference. Conclusion Children and adolescents with phenylketonuria having a strict diet can be at risk of functional vitamin B12 deficiency. This deficiency can be accurately determined by measuring methylmalonic acid concentrations. Calculation of cB12 as a biochemical index did not provide additional information compared with the measurement of methylmalonic acid alone, but may be helpful for classification of some patients with increased methylmalonic acid as having adequate vitamin B12 status.

scholarly journals Vitamin B12 and Holotranscobalamin Levels in Diabetic Peripheral Neuropathy Patients

2016 ◽  
Vol 20 (1) ◽  
pp. 16-20
Author(s):  
Mohammed Noorjahan ◽  
Kandregula Madhavi ◽  
Chandran Priscilla ◽  
Shaik A Jabeen
Keyword(s):  
Peripheral Neuropathy ◽  
Diabetic Neuropathy ◽  
Vitamin B12 ◽  
Diabetic Peripheral Neuropathy ◽  
Vitamin B12 Deficiency ◽  
Biologically Active ◽  
Significant Difference ◽  
B12 Deficiency ◽  
Vitamin B12 Supplementation

ABSTRACT Diabetic neuropathy is the most common and debilitating microvascular complication leading to nontraumatic amputations. Identifying the role of vitamin B12 in the etiology of neuropathy is crucial because simple vitamin B12 replacement may reverse neurologic symptoms if confirmed. Large proportion of vegetarians and patients on metformin have been associated with lowering of vitamin B12 levels. We have undertaken study on 60 vegetarian subjects between the age of 30 and 60 years, 20 healthy controls, 20 type 2 diabetes mellitus patients who were on metformin with peripheral neuropathy (DMWN), and 20 diabetics without peripheral neuropathy (DMWON). Vitamin B12, homocysteine, and folate levels were measured, and holotranscobalamin (holoTC) is also included, to improve the diagnosis of tissue vitamin B12 status, as holoTC is the biologically active fraction and more sensitive index of vitamin B12 status than total vitamin B12 itself. Complete history and clinical evaluation for neuropathy and electroneuromyography were recorded. Results In the DMWN group, vitamin B12 levels were significantly higher than in the DMWON group and controls [1013 (564-1501) pmol/l, 387 (245-731) pmol/l, and 305 (234-472) pmol/l respectively]. Similarly, serum holoTC was also found to be significantly higher in the DMWN [203.8 (129-300) pmol/l] group compared with the DMWON [94.4 (42.2-230.6) pmol/l] group and controls [74 (40-170) pmol/l]. Whereas mean levels of homocysteine and folate did not show any significant difference between groups, significant increase in fasting blood sugar and HbA1c was seen in the DMWN group compared with DMWON group and controls. Duration of diabetes is also significantly more in DMWN group compared with DMWON group. Conclusion We found that vitamin B12 and holoTC levels were high in patients with DMWN and DMWON groups compared with controls. Our study demonstrated that peripheral neuropathy was not associated with vitamin B12 deficiency in diabetics. These findings merit further research on a larger population to investigate into the cause of diabetic neuropathy, the factors involved, and benefit of vitamin B12 supplementation in these patients. Key messages Vitamin B12 status has no association with diabetic peripheral neuropathy. How to cite this article Noorjahan M, Madhavi K, Priscilla C, Jabeen SA. Vitamin B12 and Holotranscobalamin Levels in Diabetic Peripheral Neuropathy Patients. Indian J Med Biochem 2016;20(1):16-20.

scholarly journals Prevalence of diabetes in the adult population of Novosibirsk

2017 ◽  
Vol 20 (5) ◽  
pp. 329-334 ◽  
Author(s):  
Svetlana V. Mustafina ◽  
Oksana D. Rymar ◽  
Sofiya K. Malyutina ◽  
Diana V. Denisova ◽  
Liliya V. Shcherbakova ◽  
...  
Keyword(s):  
Adult Population ◽  
Population Sample ◽  
Age Groups ◽  
Epidemiological Studies ◽  
High Rate ◽  
Age Group ◽  
Men And Women ◽  
Younger Age ◽  
Significant Difference ◽  
High Prevalence

Aims. To estimate the prevalence of type 2 diabetes mellitus (T2DM) in different age groups of the adult population of Novosibirsk, according to epidemiological studies in 20032005 and 20132016. Methods. We examined a representative population sample (assessed in 20032005) of men and women aged 4569 years in two administrative districts of Novosibirsk, as a part of the international HAPIEE project. According to the tables of random numbers, representative samples of men and women aged 4569 years were formed, to which letters were sent, inviting them to pass for examination. During 20132016, a second population survey was conducted on a random representative sample of a population of 2544-year-olds of both sexes. Participants were residents of one of the districts of Novosibirsk. T2DM and impaired fasting glucose (IFG) were diagnosed using fasting plasma glucose (FPG) levels (diabetes: FPG 7.0 mmol/l; IFG: FPG 6.16.9 mmol/l). Results. The prevalence of T2DM among residents aged 4569 years was 11.3%, and overall, no significant difference in prevalence was found between females and males (11.3% vs. 11.0%). However, the overall prevalence of T2DM among residents aged 2544 years was 2.2%, and prevalence was higher in men (3.5%) than in women (1.1%), p 0.05. High prevalence of IFG was found in the 4569 age group (18.2%, in 20032005), and in the younger age group of 2544 years (21.6%, in 20132016). The high rate among young individuals is particularly alarming. Less than one half (4.8 out of 11.3%) of participants aged 4569 tested positive for T2DM, and only one in the age group 2544 years knew he/she had diabetes. This indicates a lack of knowledge among Siberians about their problem with diabetes. Conclusion. Approximately one in five adults had IFG. Among the adult population aged 4569 (in 20032005), 18.2% had IFG and 11.3% had T2DM. In individuals aged 2544 years in 20132016, IFG was observed in 21.6%, and 2.2% had T2DM (p0.05).

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