Molecular taxonomy reveals the sexual morph of Nodulosphaeria digitalis in Phaeosphaeriaceae from Campanula trachelium in Italy

Phytotaxa ◽  
2019 ◽  
Vol 400 (1) ◽  
pp. 1 ◽  
Author(s):  
NAPALAI CHAIWAN ◽  
DHANUSHKA NADEESHAN WANASINGHE ◽  
ERIO CAMPORESI ◽  
SAOWALUCK TIBPROMMA ◽  
SARANYAPHAT BOONMEE ◽  
...  
Keyword(s):  
Sequence Data ◽  
Molecular Taxonomy ◽  
First Record ◽  
Bootstrap Support ◽  
The Novel ◽  
Dna Sequence Data ◽  
Sexual Morph ◽  
The Family ◽  
Strong Bootstrap Support ◽  
Fungal Genus

The fungal genus Nodulosphaeria is one of the oldest phaeosphaeriaceous genera that comprises saprobic, endophytic and pathogenic species associated with a wide variety of substrates. There are 71 species epithets listed under Nodulosphaeria in Index Fungorum and most of these published records lack illustrations and descriptions, or DNA sequence data and thus it is challenging to confirm their names or investigate taxonomic relationships. In this study, we report the sexual morph of Nodulosphaeria digitalis, a terrestrial saprobe belonging to the genus Nodulosphaeria (Phaeosphaeriaceae, Pleosporales), on stems of Campanula trachelium from Arezzo Province, Italy. This is the first record of a Nodulosphaeria taxon on Campanulaceae. Maximum likelihood, Maximum parsimony and Bayesian inference analyses of combined ITS, LSU, SSU and TEF1-α sequence data reveal that the novel isolate belongs to the family Phaeosphaeriaceae and nests with Nodulosphaeria digitalis with strong bootstrap support. The detailed descriptions and illustrations of the sexual morph of N. digitalis, an updated phylogenetic tree for the genus Nodulosphaeria are provided.

scholarly journals Just a Fragment of Undescribed Diversity: Twenty New Oriental and Palearctic Species of Sciaroidea (Diptera), including DNA Sequence Data and Two New Fossil Genera

Insects ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 19
Author(s):  
Jan Ševčík ◽  
Heikki Hippa ◽  
Nikola Burdíková
Keyword(s):  
Type Species ◽  
Sequence Data ◽  
First Record ◽  
New Genera ◽  
Burmese Amber ◽  
Mitochondrial Coi ◽  
Dna Sequence Data ◽  
Incertae Sedis ◽  
The Family ◽  
Palearctic Species

The following 17 extant new species of Sciaroidea (Diptera: Bibionomorpha) are described: Bolitophila nikolae Ševčík sp. nov. (Bolitophilidae, Taiwan), Catocha jingfui sp. nov. (Cecidomyiidae, Taiwan), Catocha manmiaoe sp. nov. (Cecidomyiidae, Taiwan), Catocha shengfengi sp. nov. (Cecidomyiidae, Taiwan), Planetella taiwanensis sp. nov. (Cecidomyiidae, Taiwan), Diadocidia pseudospinusola sp. nov. (Diadocidiidae, Taiwan), Asioditomyia bruneicola sp. nov. (Ditomyiidae, Brunei), Asioditomyia lacii sp. nov. (Ditomyiidae, Taiwan), Ditomyia asiatica sp. nov. (Ditomyiidae, Thailand), Chetoneura davidi sp. nov. (Keroplatidae, Brunei), Euceroplatus mantici sp. nov. (Keroplatidae, Thailand), Setostylus fangshuoi sp. nov. (Keroplatidae, Taiwan), Platyceridion yunfui sp. nov. (Keroplatidae, Hainan), Terocelion adami sp. nov. (Keroplatidae, Taiwan), Hadroneura martini sp. nov. (Mycetophilidae, Taiwan), Paratinia furcata sp. nov. (Mycetophilidae, Czech Republic, Slovakia), and Nepaletricha sikorai sp. nov. (Sciaroidea incertae sedis, Thailand). Two new genera are described from the mid-Cretaceous Burmese amber, Burmasymmerus gen. nov. (Ditomyiidae, type species Burmasymmerus korneliae sp. nov., including also B. wieslawi sp. nov.), representing the first record of the family Ditomyiidae from the Mesozoic, and Burmatricha gen. nov. (Sciaroidea incertae sedis, type species Burmatricha mesozoica sp. nov.). Molecular phylogeny of Ditomyiidae, based on two DNA markers (28S, COI), as well as that of Catocha Haliday, 1833, based on the mitochondrial COI and 16S fragments, are also presented.

scholarly journals Sequence data from isolated lichen-associated melanized fungi enhance delimitation of two new lineages within Chaetothyriomycetidae

2021 ◽  
Vol 20 (7) ◽  
pp. 911-927
Author(s):  
Lucia Muggia ◽  
Yu Quan ◽  
Cécile Gueidan ◽  
Abdullah M. S. Al-Hatmi ◽  
Martin Grube ◽  
...  
Keyword(s):  
Sequence Data ◽  
Single Species ◽  
Sister Group ◽  
Asexual Propagation ◽  
Dna Sequence Data ◽  
Wide Range ◽  
The Family ◽  
Rock Inhabiting Fungi ◽  
Stable Habitat

AbstractLichen thalli provide a long-lived and stable habitat for colonization by a wide range of microorganisms. Increased interest in these lichen-associated microbial communities has revealed an impressive diversity of fungi, including several novel lineages which still await formal taxonomic recognition. Among these, members of the Eurotiomycetes and Dothideomycetes usually occur asymptomatically in the lichen thalli, even if they share ancestry with fungi that may be parasitic on their host. Mycelia of the isolates are characterized by melanized cell walls and the fungi display exclusively asexual propagation. Their taxonomic placement requires, therefore, the use of DNA sequence data. Here, we consider recently published sequence data from lichen-associated fungi and characterize and formally describe two new, individually monophyletic lineages at family, genus, and species levels. The Pleostigmataceae fam. nov. and Melanina gen. nov. both comprise rock-inhabiting fungi that associate with epilithic, crust-forming lichens in subalpine habitats. The phylogenetic placement and the monophyly of Pleostigmataceae lack statistical support, but the family was resolved as sister to the order Verrucariales. This family comprises the species Pleostigma alpinum sp. nov., P. frigidum sp. nov., P. jungermannicola, and P. lichenophilum sp. nov. The placement of the genus Melanina is supported as a lineage within the Chaetothyriales. To date, this genus comprises the single species M. gunde-cimermaniae sp. nov. and forms a sister group to a large lineage including Herpotrichiellaceae, Chaetothyriaceae, Cyphellophoraceae, and Trichomeriaceae. The new phylogenetic analysis of the subclass Chaetothyiomycetidae provides new insight into genus and family level delimitation and classification of this ecologically diverse group of fungi.

scholarly journals Characterization and Pathogenicity of Botryosphaeriaceae Species Collected from Olive and Other Hosts in Spain and California

2010 ◽  
Vol 100 (12) ◽  
pp. 1340-1351 ◽  
Author(s):  
Juan Moral ◽  
Concepción Muñoz-Díez ◽  
Nazaret González ◽  
Antonio Trapero ◽  
Themis J. Michailides
Keyword(s):  
Sequence Data ◽  
Close Association ◽  
Elongation Factor ◽  
Fruit Rot ◽  
Disease Symptoms ◽  
Dna Sequence Data ◽  
Olive Fly ◽  
The Family ◽  
Pathogenicity Tests ◽  
Olive Trees

Species in the family Botryosphaeriaceae are common pathogens causing fruit rot and dieback of many woody plants. In this study, 150 Botryosphaeriaceae isolates were collected from olive and other hosts in Spain and California. Representative isolates of each type were characterized based on morphological features and comparisons of DNA sequence data of three regions: internal transcribed spacer 5.8S, β-tubulin, and elongation factor. Three main species were identified as Neofusicoccum mediterraneum, causing dieback of branches of olive and pistachio; Diplodia seriata, causing decay of ripe fruit and dieback of olive branches; and Botryosphaeria dothidea, causing dalmatian disease on unripe olive fruit in Spain. Moreover, the sexual stage of this last species was also found attacking olive branches in California. In pathogenicity tests using unripe fruit and branches of olive, D. seriata isolates were the least aggressive on the fruit and branches while N. mediterraneum isolates were the most aggressive on both tissues. Isolates of B. dothidea which cause dalmatian disease on fruit were not pathogenic on branches and only weakly aggressive on fruit. These results, together with the close association between the presence of dalmatian disease symptoms and the wound created by the olive fly (Bactrocera oleae), suggest that the fly is essential for the initiation of the disease on fruit. Isolates recovered from dalmatian disease symptoms had an optimum of 26°C for mycelial growth and 30°C for conidial germination, suggesting that the pathogen is well adapted to high summer temperatures. In contrast, the range of water activity in the medium for growth of dalmatian isolates was 0.93 to 1 MPa, which was similar to that for the majority of fungi. This study resolved long-standing questions of identity and pathogenicity of species within the family Botryosphaeriaceae attacking olive trees in Spain and California.

Description of the immature stages of the Neotropical whirligig beetle Gyrinus (Neogyrinus) rozei Ochs, 1953 (Coleoptera: Gyrinidae) and first report of the parasitoid wasp Melanosmicra sp. (Hymenoptera: Chalcididae) on a Gyrinus species

Zootaxa ◽  
2020 ◽  
Vol 4732 (1) ◽  
pp. 99-116
Author(s):  
DANIARA COLPANI ◽  
CÉSAR JOÃO BENETTI ◽  
NEUSA HAMADA ◽  
VANDERLY ANDRADE-SOUZA ◽  
KARINE SCHOENINGER ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Parasitoid Wasp ◽  
First Record ◽  
The Body ◽  
Immature Stages ◽  
Cytochrome Oxidase Subunit ◽  
Dna Sequence Data ◽  
Gene Coi ◽  
Northern Brazil

In most species of Gyrinidae, the immature stages are unknown, especially due to the difficulty in collecting the juveniles and assigning them to a particular species. Molecular association is a feasible technique that may solve this problem. Recent studies have used DNA sequence data, specifically the gene cytochrome oxidase subunit I (COI), to associate immature and adult stages, thus enabling the description of the former. The objectives of this study were (1) to describe and illustrate the immature stages of Gyrinus (Neogyrinus) rozei Ochs, 1953 including morphometric, chaetotaxic and bionomic information, and (2) to assess the usefulness of the gene COI to associate immatures and adults of Gyrinus. The studied specimens were collected in Roraima state, northern Brazil. The association of immature and adult stages was done either by rearing adults under laboratory conditions or by using DNA sequence data (COI). Eggs were described based on scanning electron microscopy; they are distinguished mainly by several features of chorion, micropyle and reticulation. Larvae of G. (N.) rozei can be distinguished from those of other Neotropical Gyrinidae by a combination of several characters, including the stipes with five robust hook-like additional setae on the dorsointernal margin, and the lacinia roughly hook-shaped. The pupa is similar to that of G. argentinus Steinhel, 1869, except for the number of setae on the body. The first record of the parasitoid wasp Melanosmicra sp. (Hymenoptera: Chalcididae) on a Gyrinus species is also provided. 

scholarly journals First sexual morph record of Sarcopodium vanillae

Mycotaxon ◽  
2020 ◽  
Vol 134 (4) ◽  
pp. 707-717
Author(s):  
Napalai Chaiwan ◽  
Sajeewa S.N. Maharachchikumbura ◽  
Dhanushka N. Wanasinghe ◽  
Mingkwan Doilom ◽  
Ruvishika Jayawardena ◽  
...  
Keyword(s):  
Sequence Data ◽  
First Record ◽  
Dead Leaf ◽  
Single Spore ◽  
Sexual Morph

Sarcopodium vanillae was isolated from a dead leaf of Dracaena in Chiang Rai Province, Thailand. Combined analyses of ACT, ITS, LSU, and TUB2 sequence data obtained from the cultures derived from single spore isolates confirm that our collections represent S. vanillae. This is the first record of the sexual morph, and the first record of S. vanillae from Dracaena. A description and illustrations of both sexual and asexual stages of S. vanillae are provided.

The generic classification of the Trochilini (Aves: Trochilidae): Reconciling taxonomy with phylogeny

Zootaxa ◽  
2017 ◽  
Vol 4353 (3) ◽  
pp. 401 ◽  
Author(s):  
F. GARY STILES ◽  
J. V. JR. REMSEN ◽  
JIMMY A. MCGUIRE
Keyword(s):  
New Genus ◽  
Sequence Data ◽  
Historical Review ◽  
Genetic Data ◽  
Zoological Nomenclature ◽  
Tabular Form ◽  
Dna Sequence Data ◽  
The Family ◽  
Traditional Classification

The generic nomenclature of the hummingbirds is unusually complicated. McGuire et al.’s (2014) recent phylogeny of the Trochilidae based on DNA sequence data has greatly clarified relationships within the family but conflicts strongly with the traditional classification of the family at the genus level, especially that of the largest and most recently derived clade, the Trochilini or “emeralds”. We recently presented a historical review of this classification and the generic modifications required by the Code of the International Commission on Zoological Nomenclature. Herein we present a revised generic classification of the Trochilini based upon McGuire et al.’s genetic data, while producing diagnosable generic groupings and preserving nomenclatural stability insofar as possible. However, this generic rearrangement has necessitated the resurrection of nine generic names currently considered synonyms, the synonymization of seven currently recognized genera and the creation of one new genus. The generic changes we recommend to the classification are drastic, and we summarize these in tabular form in comparison with the three most recent classifications of the Trochilini. Where appropriate, we outline alternatives to our proposed arrangement. The classification treats 110 species in 35 genera, including two species that remain unplaced for lack of genetic samples. 

Perssoniella and the genera of Schistochilaceae: a new classification based on molecular phylogenies

2010 ◽  
Vol 23 (4) ◽  
pp. 229 ◽  
Author(s):  
Xiaolan He ◽  
David Glenny
Keyword(s):  
Dna Sequence ◽  
New Caledonia ◽  
Sequence Data ◽  
Strong Support ◽  
Morphological Characters ◽  
Monotypic Genus ◽  
New Classification ◽  
Dna Sequence Data ◽  
The Family ◽  
Molecular Phylogenies

The monotypic genus Perssoniella with P. vitreocincta Herzog, endemic to New Caledonia, possesses a series of unique morphological characters and it has been assumed that the genus, assigned to the family Perssoniellaceae and suborder Perssoniellineae, is very isolated but sister to the family Schistochilaceae. The systematic identity of Perssoniella vitreocincta was studied using DNA sequence data for the chloroplast rbcL, rps4 and trnL-F regions. Our analyses placed Perssoniella vitreocincta within the family Schistochilaceae, and within Schistochila itself, with strong support. It suggests that retaining Perssoniella as an independent genus is untenable and we transfer it to the genus Schistochila. Our results indicate that Perssoniella vitreocincta is not an archaic species, as presupposed earlier. The differentiating characters in Perssoniella are mostly probably later derived, rather than ancestral. Our analyses also placed Pachyschistochila and Paraschistochila within Schistochila, again with strong support. We also transfer these two genera to Schistochila.

Floral organogenesis in Dysosma versipellis (Berberidaceae) and its systematic implications

Botany ◽  
2016 ◽  
Vol 94 (5) ◽  
pp. 359-368 ◽  
Author(s):  
Liang Zhao ◽  
Julien B. Bachelier ◽  
Xiao-hui Zhang ◽  
Yi Ren
Keyword(s):  
Common Ancestor ◽  
Sequence Data ◽  
Floral Organogenesis ◽  
Dna Sequence Data ◽  
Sister Clade ◽  
Dysosma Versipellis ◽  
The Family ◽  
The Common ◽  
Flower Ontogeny ◽  
Developmental Features

The Berberidaceae and the six other families of Ranunculales form a sister clade to all other eudicots, and are crucial to reconstructing the common ancestor of flowering plants. Previous studies have suggested that the petals of most Berberidaceae are derived from stamens, and some are thought to develop petals from common petal/stamen primordia. However, the flower ontogeny is still poorly known in the family and the presence of common primordia needs to be re-evaluated from a comparative developmental perspective. Here, we used scanning electron microscopy to study the floral development of the endemic Chinese species Dysosma versipellis (Hance) M. Cheng ex Ying, which was originally placed in Podophyllum. Our results show that the floral organs are all free and the sepals, petals, and stamens are initiated centripetally in successive and alternate trimerous pairs of whorls around a single carpel. The nectarless petals are initiated separately and do not develop from common primordia with the stamens. Floral and developmental features of D. versipellis are similar to those of most members of Berberidaceae. The regular development of multiple flowers and absence of a secondary increase in the number of stamens in Dysosma support its exclusion from Podophyllum, and this is also inferred by the DNA sequence data.

Molecular phylogeny and diversity of Laburnicola (Didymosphaeriaceae): a new species from Uzbekistan

Phytotaxa ◽  
2021 ◽  
Vol 527 (3) ◽  
pp. 177-190
Author(s):  
ZIN HNIN HTET ◽  
AUSANA MAPOOK ◽  
YUSUFJON GAFFOROV ◽  
K.W. THILINI CHETHANA ◽  
SAISAMORN LUMYONG ◽  
...  
Keyword(s):  
New Species ◽  
Maximum Likelihood ◽  
First Record ◽  
Bootstrap Support ◽  
Distribution Map ◽  
Species List ◽  
Bayesian Analyses ◽  
Strong Bootstrap Support ◽  
Subalpine Region ◽  
A New Species

Laburnicola is a genus in Didymosphaeriaceae that includes saprobic and endophytic fungal taxa. The current study conducted in the subalpine region of Uzbekistan discovered a new species on a dead stem of a wild rose plant. Maximum likelihood and Bayesian analyses of combined LSU, SSU, ITS, and TEF 1-ɑ dataset confirmed the new species’ taxonomic position in Laburnicola. Our new species, Laburnicola zaaminensis (TASM 6152), was clustered with L. dactylidis (MFLUCC 16-0285) with strong bootstrap support. A detailed description together with illustrations are provided for Laburnicola zaaminensis. Furthermore, an annotated species list, a distribution map, and a taxonomic key for Laburnicola species are provided. This is the first record of Laburnicola from Central Asia.

scholarly journals On the monophyly of subfamily Tectarioideae (Polypodiaceae) and the phylogenetic placement of some associated fern genera 

Phytotaxa ◽  
2014 ◽  
Vol 164 (1) ◽  
pp. 1 ◽  
Author(s):  
FA-GUO WANG ◽  
SAM BARRATT ◽  
WILFREDO FALCÓN ◽  
MICHAEL F. FAY ◽  
SAMULI LEHTONEN ◽  
...  
Keyword(s):  
New Caledonia ◽  
Sequence Data ◽  
Intergenic Spacer ◽  
Plastid Dna ◽  
Dna Sequence Data ◽  
Phylogenetic Placement ◽  
The Past ◽  
The Family ◽  
The Caribbean ◽  
First Time

The fern genus Tectaria has generally been placed in the family Tectariaceae or in subfamily Tectarioideae (placed in Dennstaedtiaceae, Dryopteridaceae or Polypodiaceae), both of which have been variously circumscribed in the past. Here we study for the first time the phylogenetic relationships of the associated genera Hypoderris (endemic to the Caribbean), Cionidium (endemic to New Caledonia) and Pseudotectaria (endemic to Madagascar and Comoros) using DNA sequence data. Based on a broad sampling of 72 species of eupolypods I (= Polypodiaceae sensu lato) and three plastid DNA regions (atpA, rbcL and the trnL-F intergenic spacer) we were able to place the three previously unsampled genera. Our results show that Cionidium, like Ctenitopsis, Fadyenia, Hemigramma and Quercifilix, is embedded in Tectaria, and the monophyly of Tectaria is therefore corroborated only if these segregate genera are included. Hypoderris is sister to Tectaria brauniana and together they are sister to Triplophyllum, which was found to be monophyletic. Despite their morphological similarity with Tectaria, the genera Pleocnemia and Pseudotectaria were placed in Dryopteridoideae. Polypodiaceae subfamily Tectarioideae (former family Tectariaceae) is hereby defined to include Arthropteris, Hypoderris, Pteridrys, Tectaria and Triplophyllum. Aenigmopteris may also belong here, but this genus remains unsampled.

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