Porous hydroxyapatite ceramics coated with biomimetic peptides for induced osteogenesis

Biomimetic peptide has attracted extensive attention in bone tissue repairing owing to its excellent biocompatibility and stability. Hydroxyapatite ceramics (HAP) possess both excellent mechanical properties and good biocompatibility. To study the effects of bionic peptide D9KIPKAS(pSer)VPTELSAISRGDS on the interfacial activity and biological properties of hydroxyapatite ceramics, porous HAP ceramics were prepared using ammonium carbonate as a pore-forming agent. To explore the influence of surface modification on the interfacial activity of porous HAP ceramics when applying different methods, surface modification was carried out using physical adsorption (HAP-p-PP2) and a chemically grafted polypeptide (HAP-c-PP2). X-ray diffraction was used to characterize the crystal morphology of the porous HAP ceramics before and after sintering. The results of FTIR and XPS showed that bionic peptides were successfully grafted onto the surface of a porous HAP ceramic. An SEM graph shows the adhesion and spread of BMSCs on the materials. Meanwhile, the results of in vitro cell experiments showed that HAP-c-PP2 can better promote BMSC proliferation. In conclusion, bionic peptide D9KIPKAS(pSer)VPTELSAISRGDS with multifunctional functional groups is more conducive to the adhesion, proliferation and differentiation of BMSCs which can make it play an effective role in osteoinduction in bone tissue engineering.

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Porous Hydroxyapatite/Chitosan/Carboxymethyl Cellulose Scaffolds with Tunable Microstructures for Bone Tissue Engineering

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.819.9 ◽

2019 ◽

Vol 819 ◽

pp. 9-14 ◽

Cited By ~ 1

Author(s):

Kanharit Wongsawichai ◽

Arada Kingkaew ◽

Aninart Pariyaisut ◽

Supang Khondee

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Carboxymethyl Cellulose ◽

Cell Attachment ◽

Biological Properties ◽

Porous Hydroxyapatite ◽

Porous Microstructure

Bone tissue engineering is an alternative approach to generate bone using biomaterials and cells. Hydroxyapatite (HA) has good biocompatibility, osteoinductivity, and osteoconductivity. However, it has limited utility due to poor mechanical properties and slow degradation rate. To improve mechanical properties and to modify degradation profile, hydroxyapatite was tethered in chitosan (CS) and carboxymethyl cellulose (CMC) complex. Gelatin was incorporated to promote cell attachment and polyvinyl alcohol (PVA) was used to improve mechanical strength of this scaffold. The physico-mechanical and biological properties of these scaffolds were investigated. Fourier transform infrared (FTIR) analysis and X-ray diffraction (XRD) showed the incorporation of hydroxyapatite in polymer matrix. The scaffolds had density, compressive strength, and Young’s modulus in the range of 0.24-0.30 g/cm3, 0.028-0.035 MPa, 0.178-0.560 MPa, respectively. The scaffolds had porosity of 69-91 percent. Higher content of PVA decreased porosity of scaffolds. Scanning electron microscope showed porous microstructure with pore size in the range of 60-183 μm. In vitro test on MC3T3-E1 preosteoblast cells showed negligible cytotoxicity of scaffolds. The data suggested that HA/CS/CMC/gelatin/PVA scaffold has potential applications in bone tissue engineering.

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In vitro Behaviour of Alumina-Hydroxiapatite Composites Coatings

Revista de Chimie ◽

10.37358/rc.18.6.6336 ◽

2018 ◽

Vol 69 (6) ◽

pp. 1416-1418

Author(s):

Alexandru Szabo ◽

Ilare Bordeasu ◽

Ion Dragos Utu ◽

Ion Mitelea

Keyword(s):

Pure Titanium ◽

Titanium Substrate ◽

High Strength ◽

Biological Properties ◽

Commercially Pure Titanium ◽

Hvof Spraying ◽

Before And After ◽

Sbf Solution ◽

Oxygen Fuel

Hydroxyapatite (HA) is a very common material used for biomedical applications. Usually, in order to improve its poor mechanical properties is combined or coated with other high-strength materials.The present paper reports the manufacturing and the biocompatibility behaviour of two different biocomposite coatings consisting of alumina (Al2O3) and hydroxyapatite (HA) using the high velocity oxygen fuel (HVOF) spraying method which were deposited onto the surface of a commercially pure titanium substrate. The biological properties of the Al2O3-HA materials were evaluated by in vitro studies. The morphology of the coatings before and after their immersing in the simulated body fluid (SBF) solution was characterized by scanning electron microscopy (SEM). The results showed an important germination of the biologic hydroxyapatite crystallite on the surface of both coatings.

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Assessment of Titanate Nanolayers in Terms of Their Physicochemical and Biological Properties

Materials ◽

10.3390/ma14040806 ◽

2021 ◽

Vol 14 (4) ◽

pp. 806

Author(s):

Michalina Ehlert ◽

Aleksandra Radtke ◽

Katarzyna Roszek ◽

Tomasz Jędrzejewski ◽

Piotr Piszczek

Keyword(s):

Surface Modification ◽

Substrate Surface ◽

Biological Properties ◽

Structure Characterization ◽

Porous Coating ◽

Apatite Formation ◽

Sem Images ◽

Energy Dispersion Spectroscopy ◽

X Ray

The surface modification of titanium substrates and its alloys in order to improve their osseointegration properties is one of widely studied issues related to the design and production of modern orthopedic and dental implants. In this paper, we discuss the results concerning Ti6Al4V substrate surface modification by (a) alkaline treatment with a 7 M NaOH solution, and (b) production of a porous coating (anodic oxidation with the use of potential U = 5 V) and then treating its surface in the abovementioned alkaline solution. We compared the apatite-forming ability of unmodified and surface-modified titanium alloy in simulated body fluid (SBF) for 1–4 weeks. Analysis of the X-ray diffraction patterns of synthesized coatings allowed their structure characterization before and after immersing in SBF. The obtained nanolayers were studied using Raman spectroscopy, diffuse reflectance infrared Fourier transform spectroscopy (DRIFT), and scanning electron microscopy (SEM) images. Elemental analysis was carried out using X-ray energy dispersion spectroscopy (SEM EDX). Wettability and biointegration activity (on the basis of the degree of integration of MG-63 osteoblast-like cells, L929 fibroblasts, and adipose-derived mesenchymal stem cells cultured in vitro on the sample surface) were also evaluated. The obtained results proved that the surfaces of Ti6Al4V and Ti6Al4V covered by TiO2 nanoporous coatings, which were modified by titanate layers, promote apatite formation in the environment of body fluids and possess optimal biointegration properties for fibroblasts and osteoblasts.

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Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Polymers ◽

10.3390/polym12010061 ◽

2020 ◽

Vol 12 (1) ◽

pp. 61 ◽

Cited By ~ 6

Author(s):

Yannan Liu ◽

Juan Gu ◽

Daidi Fan

Keyword(s):

Mechanical Properties ◽

Enzymatic Hydrolysis ◽

Bone Tissue ◽

Tissue Regeneration ◽

Bone Repair ◽

Three Dimensional ◽

High Strength ◽

Biological Properties ◽

Bone Tissue Regeneration

A novel, three-dimensional, porous, human-like collagen (HLC)/nano-hydroxyapatite (n-HA) scaffold cross-linked by 1,2,7,8-diepoxyoctane (DEO) was successfully fabricated, which showed excellent mechanical and superior biological properties for bone tissue regeneration in this study. The physicochemical characterizations of different n-HA/HLC/DEO (nHD) scaffolds were investigated by determining the morphology, compression stress, elastic modulus, Young’s modulus and enzymatic hydrolysis behavior in vitro. The results demonstrated that nHD-2 and nHD-3 scaffolds showed superior mechanical properties and resistance to enzymatic hydrolysis compared to nHD-1 scaffolds. The cell viability, live cell staining and cell adhesion analysis results demonstrated that nHD-2 scaffolds exhibited low cytotoxicity and excellent cytocompatibility compared with nHD-1 and nHD-3 scaffolds. Furthermore, subcutaneous injections of nHD-2 scaffolds in rabbits produced superior anti-biodegradation effects and histocompatibility compared with injections of nHD-1 and nHD-3 scaffolds after 1, 2 and 4 weeks. In addition, the repair of bone defects in rabbits demonstrated that nHD-2 scaffolds presented an improved ability for guided bone regeneration and reconstruction compared to commercially available bone scaffold composite hydroxyapatite/collagen (HC). Collectively, the results show that nHD-2 scaffolds show promise for application in bone tissue engineering due to their excellent mechanical properties, anti-biodegradation, anti-biodegradation, biocompatibility and bone repair effects.

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Cuttlefish Bone-Derived Biphasic Calcium Phosphate Scaffolds Coated with Sol-Gel Derived Bioactive Glass

Materials ◽

10.3390/ma12172711 ◽

2019 ◽

Vol 12 (17) ◽

pp. 2711

Author(s):

Ana S. Neto ◽

Daniela Brazete ◽

José M.F. Ferreira

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Calcium Phosphates ◽

Sol Gel ◽

Biological Properties ◽

X Ray Diffraction ◽

Bioactive Glasses ◽

Hydrothermal Transformation

The combination of calcium phosphates with bioactive glasses (BG) has received an increased interest in the field of bone tissue engineering. In the present work, biphasic calcium phosphates (BCP) obtained by hydrothermal transformation of cuttlefish bone (CB) were coated with a Sr-, Mg- and Zn-doped sol-gel derived BG. The scaffolds were characterized by X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. The initial CB structure was maintained after hydrothermal transformation (HT) and the scaffold functionalization did not jeopardize the internal structure. The results of the in-vitro bioactivity after immersing the BG coated scaffolds in simulated body fluid (SBF) for 15 days showed the formation of apatite on the surface of the scaffolds. Overall, the functionalized CB derived BCP scaffolds revealed promising properties, but further assessment of the in-vitro biological properties is needed before being considered for their use in bone tissue engineering applications.

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In Vitro Cytotoxicity Study of the Nano-Hydroxyapatite/Bacterial Cellulose Nanocomposites

Materials Science Forum ◽

10.4028/www.scientific.net/msf.610-613.1011 ◽

2009 ◽

Vol 610-613 ◽

pp. 1011-1016 ◽

Cited By ~ 3

Author(s):

Yan Mei Chen ◽

Ting Fei Xi ◽

Yu Dong Zheng ◽

Yi Zao Wan

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bacterial Cellulose ◽

Bone Tissue ◽

Biological Response ◽

Biological Properties ◽

In Vitro Cytotoxicity ◽

National Standard ◽

Target Cells

The nanocomposite of nano-hydroxyapatite/bacterial cellulose (nHA/BC) obtained by depositing in simulated body fluid (SBF), incorporating their excellent mechanical and biological properties, is expected to have potential applications in bone tissue engineering. However, the biological response evaluation of biomaterials is required to provide useful information to improve their design and application. In this article, the in vitro cytotoxicity of composites nHA/BC as well as its degradation residues was studied. Scanning electron microscopy (SEM) was used to observe the morphology of original materials and their degradation residues. The degree of degradation was evalued by measuring the concentration of reducing sugar (glucose) by ultraviolet spectrophotometer. Bone-forming osteoblasts (OB) and infinite culture cell line L929 fibroblasts were used to measure the cytotocixity of materials with MTT assay. Both kinds of cells in infusion proliferate greatly in a normal form and their relative growth rate (RGR) exceeds by 75%, which shows the cytotoxicity of materials is graded as 0~1, according to the national standard. Nevertheless, bone-forming OB cells, as a kind of target cells, are more susceptive on the cytotoxicity than infinite culture fibroblast cells L929. The results suggest the nanocomposite of nHA/BC without cytotoxicity is greatly promising as a kind of scaffold materials for bone tissue engineering and tissue functional cells are more suited to evaluate the cytotoxicity of biomedical materials.

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Enhancing in vitro bioactivity and in vivo osteogenesis of organic–inorganic nanofibrous biocomposites with novel bioceramics

Journal of Materials Chemistry B ◽

10.1039/c4tb00889h ◽

2014 ◽

Vol 2 (37) ◽

pp. 6293-6305 ◽

Cited By ~ 15

Author(s):

Tao Liu ◽

Xinbo Ding ◽

Dongzhi Lai ◽

Yongwei Chen ◽

Ridong Zhang ◽

...

Keyword(s):

Bone Tissue ◽

Tissue Regeneration ◽

Biological Properties ◽

Bone Tissue Regeneration ◽

In Vitro Bioactivity ◽

Physicochemical And Biological Properties

MGHA-introduced, an electrospun SF-based composite can exhibit improved physicochemical and biological properties to stimulate bone tissue regeneration and repair.

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The Effect of Pore Forming Agent Particle Size on the Porosity, Microstructure and In Vitro Studies of Hydroxyapatite Ceramics

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.493-494.277 ◽

2011 ◽

Vol 493-494 ◽

pp. 277-280 ◽

Cited By ~ 1

Author(s):

V. Zalite ◽

Janis Locs ◽

D. Vempere ◽

Liga Berzina-Cimdina

Keyword(s):

Particle Size ◽

Fibroblast Cell ◽

Particle Size Effect ◽

Ammonium Bicarbonate ◽

Porous Hydroxyapatite ◽

Hydroxyapatite Ceramics ◽

Ceramic Microstructure ◽

Viscous Mass

In situ viscous mass foaming with ammonium bicarbonate (NH4HCO3)as foaming agent was used for the preparation of porous hydroxyapatite ceramics. Three different particle fractions (100-300 μm, 300-500 μm, 500-1000 μm) of NH4HCO3 were used in order to evaluate the particle size effect on the ceramic microstructure. Foamed and dried green bodies were sintered at 1150°C. The porosity and microstructure of ceramics were determined with Archimedes method and scanning electron microscopy. As the NH4HCO3 particle fraction was decrease from 100-300 μm to 500-1000 μm, the open porosity increased from 42 to 54 %. In situ viscous mass foaming leads to the interconnected pore channels with wide pore size range (10-600 μm). In the present work cytotoxicity of obtained porous HA ceramics and fibroblast cell viability was investigated.

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Reinforcing of Porous Hydroxyapatite Ceramics with Hydroxyapatite Fibres for Enhanced Bone Tissue Engineering

Journal of Biomimetics Biomaterials and Tissue Engineering ◽

10.4028/www.scientific.net/jbbte.10.67 ◽

2011 ◽

Vol 10 ◽

pp. 67-73 ◽

Cited By ~ 1

Author(s):

Lie Feng Liang ◽

Xiao Yi Han ◽

Xiao Cai Yan ◽

Jie Weng

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Test Machine ◽

Porous Structures ◽

X Ray Diffraction ◽

Porous Hydroxyapatite ◽

Hydroxyapatite Ceramics ◽

Ceramic Implants

Porous hydroxyapatite (HA) ceramic implants have attracted attention in bone tissue engineering due to their excellent bioactivity and biocompatibility due to their chemical similarity with the mineral component of natural bone. Unfortunately, HA when is formed into porous structures exhibits very low compression strength. In this study, fabrication of porous HA ceramic scaffolds containing HA fibers is presented. The primary aim of the study is to improve mechanical properties of the scaffold by introducing the fiber with uniform component relative to the scaffold. Scanning electron microscopy was used to observe the surface morphology and pore size of the scaffold. X-ray diffraction (XRD) was used to detect the phase composition and crystallinity of the scaffold. The compressive strength was determined using a universal material test machine. The results and the characterizations demonstrate the addition of HA fiber could enhance the uniformity of mechanical properties among samples and also the strength for a given open porosity.

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