Histomorphological and Immunohistochemical Studies of Chronic Active Hepatitis in Doberman Pinschers

1998 ◽  
Vol 35 (5) ◽  
pp. 380-385 ◽  
Author(s):  
L. P. Thornburg
Keyword(s):  
Hepatic Vein ◽  
Chronic Active Hepatitis ◽  
Plasma Cells ◽  
Normal Liver ◽  
Scar Tissue ◽  
Hepatic Veins ◽  
Tissue Samples ◽  
Hepatic Copper ◽  
Liver Copper ◽  
Doberman Pinschers

Liver tissue samples were reviewed from 35 Doberman Pinschers with chronic active hepatitis in the precirrhotic stage. Thirty dogs had elevated hepatic copper concentrations, and five had normal liver copper concentrations. The earliest changes were inflammation and scar tissue deposition around the small hepatic vein branches. There was also apoptosis of scattered hepatocytes in zone 3. Inflammation consisted of macrophages, lymphocytes, and plasma cells. As the disease progressed, collagen deposition increased around the hepatic veins; in some liver specimens, thin scar tissue septa radiated from the hepatic vein branches, and inflammation spread to include the portal tracts. The sinusoids adjacent to the scar tissue were converted to endothelial-lined, thin-walled vessels. Chronic active hepatitis (commonly referred to as Doberman hepatitis or chronic active hepatitis of Dobermans) is a progressive fibrosis, inflammation and hepatocyte loss beginning among zone 3 hepatocytes around the terminal hepatic vein branches. The histomorphologic changes were the same among those Dobermans with elevated hepatic copper and those with normal hepatic copper. The cause was not determined, but these morphologic studies support the idea of immune-mediated disease.

Assessment of copper accumulation in archived liver specimens from cats

2020 ◽  
pp. 1098612X2096135
Author(s):  
Punyamanee Yamkate ◽  
Randi M Gold ◽  
Panagiotis G Xenoulis ◽  
Katja Steiger ◽  
David C Twedt ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Hepatic Steatosis ◽  
Dry Weight ◽  
Reference Interval ◽  
Copper Accumulation ◽  
Zonal Distribution ◽  
Tissue Samples ◽  
Hepatic Copper ◽  
Liver Copper ◽  
Flame Atomic Absorption

Objectives The aim of this study was to assess hepatic copper concentrations and zonal distribution in cat liver specimens. Methods For this study, 121 archived, formalin-fixed, paraffin-embedded liver specimens from cats were used. Tissue sections were stained for copper with rhodanine and scored from 0 (no copper accumulation) to 5 (panlobular copper accumulation). The tissue specimens were then deparaffinized and hepatic copper concentrations were measured using flame atomic absorption spectroscopy. Results Tissue samples were categorized into four groups based on histopathologic findings: (1) no significant histopathologic hepatic changes (n = 66); (2) hepatic steatosis (n = 18); (3) inflammatory or infectious disease (n = 24); and (4) neoplasia (n = 13). Of the 121 specimens, 13 (11%) stained positive for copper, with three having a score ⩾3. Thirty-seven specimens (31%) had copper concentrations above the reference interval ([RI] <180 µg/g dry weight liver). Copper concentrations in cats with hepatic inflammatory or infectious disease were significantly higher than cats with hepatic steatosis ( P = 0.03). Copper-staining score and concentration were positively correlated ( rs = 0.46, P <0.001). Conclusions and relevance Despite the fact that 31% of specimens had copper concentrations above the RI, only 11% showed positive copper staining and only 2.5% had a score ⩾3. Our findings suggest that hepatic copper concentrations greater than the upper limit of the RI are relatively common in cats. Further studies to determine the factors that influence hepatic copper staining in cats and to establish contemporary RIs for hepatic copper in healthy cats are warranted.

Hepatic transit time of ultrasound contrast in biopsy characterized liver disease

2005 ◽  
Vol 46 (6) ◽  
pp. 557-560 ◽  
Author(s):  
J. F. Pedersen ◽  
V. A. Larsen ◽  
P. Bytzer ◽  
L. G. Madsen ◽  
O. Hamberg
Keyword(s):  
Liver Disease ◽  
Transit Time ◽  
Hepatic Vein ◽  
Arrival Time ◽  
Ultrasound Contrast Agent ◽  
Normal Liver ◽  
Hepatic Veins ◽  
Ultrasound Contrast ◽  
Early Arrival ◽  
Hepatic Pathology

Purpose: To study the hepatic transit time of an ultrasound contrast agent in patients with liver disease, and to evaluate the mechanism(s) of the well-established shorter cubital vein to hepatic vein transit time in cirrhosis. Material and Methods: Thirty-four patients scheduled for Menghini liver biopsy were studied by ultrasound after injection of 2.5 g Levovist (Schering, Berlin, Germany) into an arm vein. The time from injection until the first appearance of contrast echoes in the hepatic artery and hepatic veins was registered. Hepatic transit time was the difference between the two. Results: Biopsy showed cirrhosis in 9 patients, other diffuse hepatic pathology in 23 patients, and normal liver in 2 patients. Mean hepatic vein arrival time was earlier in cirrhosis than in other liver disease (19.4 s versus 26.0 s; P = 0.013), and hepatic transit time was shorter (6.6 s versus 11.6 s; P = 0.024). A hepatic transit time <10 s was found in all patients with cirrhosis, but also in 10 of 23 patients with other liver pathology. Conclusion: Hepatic transit time measurement could not be used to distinguish between cirrhosis and other hepatic pathology, but a transit time  = 10 s excluded cirrhosis. The earlier hepatic vein arrival time in cirrhosis is apparently mainly caused by intrahepatic shunting rather than by early arrival of contrast to the liver.

Hereditary Copper Toxicosis in West Highland White Terriers

1986 ◽  
Vol 23 (2) ◽  
pp. 148-154 ◽  
Author(s):  
L. P. Thornburg ◽  
D. Shaw ◽  
M. Dolan ◽  
M. Raisbeck ◽  
S. Crawford ◽  
...  
Keyword(s):  
Copper Concentration ◽  
Normal Liver ◽  
Hepatic Necrosis ◽  
Hepatic Copper ◽  
Clinically Normal ◽  
Liver Copper ◽  
Absorption Studies ◽  
The Mean ◽  
Copper Storage ◽  
High Liver

Histologic, histochemical and atomic absorption studies on liver tissue from 71 West Highland white terriers are reported. Twenty-seven dogs had histologically normal liver and copper concentration comparable to mongrel control dogs. Forty-four dogs had hepatic copper concentrations up to 22 limes the mean copper concentration found in clinically normal mongrel dogs. Hepatitis, hepatic necrosis and cirrhosis were associated with the increased copper concentration in some dogs. Matings between dogs with high liver copper concentration produced pups with high liver concentration. The copper storage defect is inherited.

scholarly journals The Relationship between Hepatic Copper Content and Morphologic Changes in the Liver of West Highland White Terriers

1996 ◽  
Vol 33 (6) ◽  
pp. 656-661 ◽  
Author(s):  
L. P. Thornburg ◽  
G. Rottinghaus ◽  
G. Dennis ◽  
S. Crawford
Keyword(s):  
Liver Disease ◽  
Chronic Active Hepatitis ◽  
Plasma Cells ◽  
Dry Weight ◽  
Etiologic Agent ◽  
Central Vein ◽  
Copper Toxicosis ◽  
Hepatic Copper ◽  
Massive Necrosis ◽  
The Relationship

Liver tissue from 17 West Highland White Terriers (WHWTs) with cirrhosis, subacute bridging necrosis, hepatitis, or massive necrosis were examined for the presence, composition, and distribution of inflammatory foci. Copper analysis was performed on the specimens, The foci of inflammation and necrosis composed a significant part of the lesion in 15 of the samples. The foci were of two types. One, characteristic of idiopathic chronic active hepatitis, consisted of one or two apoptotic hepatocytes attended by lymphocytes and plasma cells. These foci were found primarily in the vicinity of the portal tracts, not associated with centrolobular copper-laden hepatocytes. The other type of focus was characteristic of copper toxicosis. These foci were larger and composed of debris-filled macrophages, lymphocytes, plasma cells, and scattered neutrophils, and on occasion apoptotic hepatocytes were found at the periphery. These foci were always found around the central vein among the copper-laden hepatocytes. Such foci were found only in dogs with copper concentration > 2,000 parts/million on a dry weight basis. These morphologic studies show that clinical liver disease in WHWTs is caused by more than one etiologic agent. Among 17 WHWTs with clinical liver disease, two had copper toxicosis, five had idiopathic chronic active hepatitis, and 10 had hepatic disease of undetermined type.

A RARE CASE OF COMPLICATED LIVER ABSCESS WITH MIDDLE HEPATIC VEIN THROMBOSIS: A CASE REPORT AND REVIEW OF LITERATURE

2021 ◽  
pp. 1-2
Author(s):  
V.P.S. Punia ◽  
Praveen Raman Mishra ◽  
Shaavi Mittal ◽  
Akash Bharti ◽  
Prem Kumar ◽  
...  
Keyword(s):  
Liver Abscess ◽  
Hepatic Vein ◽  
Middle Hepatic Vein ◽  
Amoebic Liver Abscess ◽  
Hepatic Veins ◽  
Review Of Literature ◽  
Extraintestinal Manifestation ◽  
Vein Thrombosis ◽  
Histolytica Infection ◽  
Life Threatening

In developing countries Amoebic liver abscess is commonly encountered disease and it’s also the commonest extraintestinal manifestation of Entamoeba histolytica infection. Usual complication of Amoebic liver abscess arises due to collection of pus in various cavities, like in peritoneal cavity following perforation, in the pleural cavity which is known as empyema thoracis, and rarely it is complicated by life threatening conditions such as venous extension of the disease involving the hepatic veins and IVC, with only few cases reported. Here we describe a case of amoebic liver abscess extending across middle hepatic vein.

Limitations of studying keloid scars using the nude athymic mouse model

2002 ◽  
Vol 10 (2) ◽  
pp. 56-61 ◽  
Author(s):  
Mp Hillmer ◽  
S Salama ◽  
Sm Macleod
Keyword(s):  
Mouse Model ◽  
Scar Tissue ◽  
In Vivo Studies ◽  
Athymic Mice ◽  
Athymic Mouse ◽  
Tissue Samples ◽  
Keloid Scars ◽  
Central Calcification ◽  
Sample Identity

Keloid scars are benign fibroproliferative growths that respond poorly to treatment. This study sought to determine the efficacy of three different glucocorticoids (triamcinolone, methylprednisolone and dexamethasone) in altering human keloid scar tissue implanted in athymic mice. Keloid tissue obtained from three patients (one man and two women) who sought cosmetic removal of their scars was implanted into athymic mice for a duration of 15 or 30 days. The keloid tissue was examined histopathologically and evaluated by a dermatopathologist who was blinded to sample identity and who was using predetermined qualitative scoring criteria. The appearance of central calcification, granulation tissue, foreign body granulomatous reaction and acute inflammatory reaction complicated the comparison of the keloid tissue samples. However, on the basis of observations reported in the present paper, it appears that triamcinolone should remain the treatment of choice for keloid scars. The athymic mouse model that is used for studying keloid scars is the best available approach to in vivo studies; however, limitations identified in this study confound the interpretation of experimental data. Ideally, promising and novel therapies should be investigated clinically.

scholarly journals AN EXPERIMENTAL STUDY INTO THE CAUSE OF THE INCREASED PORTAL PRESSURE IN PORTAL CIRRHOSIS

1907 ◽  
Vol 9 (1) ◽  
pp. 93-104 ◽  
Author(s):  
Frederick C. Herrick
Keyword(s):  
Arterial Pressure ◽  
Portal Pressure ◽  
Normal Liver ◽  
Volume Flow ◽  
Cirrhotic Liver ◽  
Return Flow ◽  
Hepatic Veins ◽  
Portal Cirrhosis ◽  
Arterial Inflow ◽  
Flow Through

1. In the liver of portal cirrhosis there is a far freer communication between the arterial and portal currents than in the normal liver. 2. Factors contributing to the increased portal pressure in portal cirrhosis are (1) the direct communication of the arterial pressure to the portal vessels through dilated capillaries, (2) the larger volume-flow of the hepatic artery in proportion to the portal flow in cirrhosis as compared to that in the normal liver. 3. A portal cirrhotic liver gives passage to an amount of portal fluid proportionate to .its weight. There is no obstruction to the portal vessels from fibrosis in the large portal cirrhotic liver. 4. From an arterial inflow there is a free return flow through the portal as well as through the hepatic veins in both normal and cirrhotic livers. 5. From a portal inflow the return is through the hepatic vein only. The Gad's theory of valves and the arterial capillary network account for this fact. 6. The portal pressure has a decided influence on the arterial volume-flow and vice versa. This influence is more marked in the cirrhotic than in the normal liver. 7. The communication of the arterial pressure to the portal pressure is an important factor in an explanation of the increased portal pressure in portal cirrhosis.

scholarly journals Diagnostic Value of Hepatic Vein Ultrasound in Early Detection of Liver Cirrhosis

2018 ◽  
Vol 7 ◽  
pp. e1140
Author(s):  
Hadi Zare Marzouni ◽  
Behrooz Davachi ◽  
Mahdi Rezazadeh ◽  
Mahmoud Salehi Milani ◽  
Sedighe Matinfard
Keyword(s):  
Chronic Hepatitis ◽  
Early Detection ◽  
Fatty Liver ◽  
Hepatic Vein ◽  
Diagnostic Value ◽  
Radiology Department ◽  
Hepatic Veins ◽  
Common Disease ◽  
Vein Wall ◽  
Ultrasound Guide

Background: Cirrhosis is a common disease that destroys liver cells, and it has various etiologies. The early diagnosis of cirrhosis can be effective in improving prognosis.Considering the availability and affordability of ultrasound devices throughout the world, in this study we evaluated the diagnostic value of hepatic vein ultrasound examination in the early detection of liver cirrhosis.Materials and Methods: In this study, 45 patients referred to the radiology department of Ghaem Hospital for liver biopsy were evaluated for changes in the hepatic veins under ultrasound with a transducer of 5-7MHz. One piece of the hepatic vein was selected for ultrasound, and the wall of hepatic veins was examined for straightness and uniformity of echogenicity. Subsequently, patients underwent biopsy by ultrasound guide.  Of all the study subjects, eight patients presented mild chronic hepatitis without fibrosis, four patients were diagnosed with fatty liver, and 33 patients had severe cirrhosis or chronic hepatitis with fibrosis.Results: Our results showed that hepatitis patients with or without fibrosis had irregular and wavy hepatic vein wall (impairment in straightness) with non-uniform echo (impairment in uniformity of echogenicity). While patients without hepatitis and cirrhosis, but diagnosed with fatty liver, had a smooth and regular hepatic vein wall with a uniform echo.Conclusion: The present study showed that hepatic vein examination in terms of echogenicity and straightness could be used to diagnose hepatitis, its severity as well as its course towards fibrosis and cirrhosis. [GMJ.2018;7:e1140]

Extended left trisectionectomy severing all hepatic veins preserving segment 6 and inferior right hepatic vein

2008 ◽  
Vol 34 (2) ◽  
pp. 247-251 ◽  
Author(s):  
M.A. Machado ◽  
T. Bacchella ◽  
F.F. Makdissi ◽  
R.T. Surjan ◽  
M.C. Machado
Keyword(s):  
Hepatic Vein ◽  
Hepatic Veins ◽  
Left Trisectionectomy ◽  
Right Hepatic Vein

scholarly journals Association between Liver Copper Concentration and Subclinical Hepatitis in Doberman Pinschers

2004 ◽  
Vol 18 (5) ◽  
pp. 647-650 ◽  
Author(s):  
P.J.J. Mandigers ◽  
T.S.G.A.M. Ingh ◽  
P. Bode ◽  
E. Teske ◽  
J. Rothuizen
Keyword(s):  
Copper Concentration ◽  
Liver Copper ◽  
Doberman Pinschers
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