Feeding Time Synchronizes Clock Gene Rhythmic Expression in Brain and Liver of Goldfish (Carassius auratus)

2011 ◽  
Vol 26 (1) ◽  
pp. 24-33 ◽  
Author(s):  
Andrea Feliciano ◽  
Yurena Vivas ◽  
Nuria de Pedro ◽  
María J. Delgado ◽  
Elena Velarde ◽  
...  
2012 ◽  
Vol 29 (6) ◽  
pp. 665-673 ◽  
Author(s):  
Laura G. Nisembaum ◽  
Elena Velarde ◽  
Ana B. Tinoco ◽  
Clara Azpeleta ◽  
Nuria de Pedro ◽  
...  

2017 ◽  
Vol 49 (2) ◽  
pp. 726-737 ◽  
Author(s):  
Ping Wu ◽  
Jun Shi ◽  
Chengyong Yang ◽  
Fangliang Zhang ◽  
Yulong Li ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141043 ◽  
Author(s):  
Aída Sánchez-Bretaño ◽  
Ayelén M. Blanco ◽  
Suraj Unniappan ◽  
Olivier Kah ◽  
Marie-M. Gueguen ◽  
...  

2018 ◽  
Vol 314 (2) ◽  
pp. R304-R312 ◽  
Author(s):  
Ayelén M. Blanco ◽  
Miguel Gómez-Boronat ◽  
Diego Madera ◽  
Ana I. Valenciano ◽  
Angel L. Alonso-Gómez ◽  
...  

Nocturnin (NOC) is a unique deadenylase with robust rhythmic expression involved in the regulation of metabolic processes in mammals. Currently, the possible presence of NOC in fish is unknown. This report aimed to identify NOC in a fish model, the goldfish ( Carassius auratus), and to study the possible regulation of its expression by feeding. Two partial-length cDNAs of 293 and 223 bp, named nocturnin-a ( noc-a) and nocturnin-b ( noc-b), were identified and found to be highly conserved among vertebrates. Both mRNAs show a similar widespread distribution in central and peripheral tissues, with higher levels detected for noc-a compared with noc-b. The periprandial expression profile revealed that noc-a mRNAs rise sharply after a meal in hypothalamus, intestinal bulb, and liver, whereas almost no changes were observed for noc-b. Food deprivation was found to exert opposite effects on the expression of both NOCs (generally inhibitory for noc-a, and stimulatory for noc-b) in the three mentioned tissues. A single meal after a 48-h food deprivation period reversed (totally or partially) the fasting-induced decreases in noc-a transcripts in all studied tissues and the increases in noc-b expression in the intestinal bulb. Together, this study offers the first report of NOC in fish and shows a high dependence of its expression on feeding and nutritional status. The differential responses to feeding of the two NOCs raise the possibility that they might be underlying different physiological mechanisms (e.g., food intake, lipid mobilization, energy homeostasis) in fish.


2014 ◽  
Vol 204 ◽  
pp. 239-247 ◽  
Author(s):  
Ana B. Tinoco ◽  
Laura G. Nisembaum ◽  
Nuria de Pedro ◽  
María J. Delgado ◽  
Esther Isorna

Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham ◽  
Johnny L. Carson

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.


2001 ◽  
Vol 36 (3-4) ◽  
pp. 195-198 ◽  
Author(s):  
I Paschos ◽  
L Natsis ◽  
C Nathanailides ◽  
I Kagalou ◽  
E Kolettas

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