Dopamine self-polymerized along with hydroxyapatite onto the preactivated titanium percutaneous implants surface to promote human gingival fibroblast behavior and antimicrobial activity for biological sealing

2018 ◽  
Vol 32 (8) ◽  
pp. 1071-1082 ◽  
Author(s):  
Minggang Yang ◽  
Peipei Jiang ◽  
Yang Ge ◽  
Fang Lan ◽  
Xuedong Zhou ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Soft Tissue ◽  
Titanium Surface ◽  
Soft Tissues ◽  
Pure Titanium ◽  
Carboxymethyl Chitosan ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Tissue Integration ◽  
Collagen Secretion

The clinical success of dental implants requires not only the optimum osseointegration but also the integration of implant surface with soft tissues to form biological sealing. In this study, alkali-heat treatment was applied to modify the pure titanium surface constructing a unique micro-and nano-structure. Then, poly(dopamine), along and with the additional incorporation of hydroxyapatite and carboxymethyl chitosan have been successfully infiltrated into the preactivated Ti surface during dopamine self-polymerization proceeding. Here, the effects of poly(dopamine)-modified surface coating on the biological behaviors of human gingival fibroblasts (HGFs) and oral pathogens have been systematically studied, which was critical for the early peri-implant soft tissue integration. The results showed that the poly(dopamine)-modified alkali-heat-titanium surface was a superior substrate for human gingival fibroblast adhesion, spread and proliferation. Moreover, further enhancements on cytoskeleton organization, collagen secretion and fibronectin adsorption were generally observed through the additional incorporation of hydroxyapatite. The addition of carboxymethyl chitosan exerted a positive modulation effect on antibacterial activity. Overall, our study demonstrated that combined superior soft tissue integration and antibacterial activity can be achieved by using poly(dopamine)-modified titanium implant, which has great potential in the optimal design of dental implant.

scholarly journals Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Zhiqiang Xu ◽  
Yuqi He ◽  
Xiufeng Zeng ◽  
Xiuxia Zeng ◽  
Junhui Huang ◽  
...  
Keyword(s):  
Dental Implants ◽  
Porphyromonas Gingivalis ◽  
Soft Tissues ◽  
Titania Nanotubes ◽  
Biological Behavior ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Related Gene ◽  
Gene Expressions ◽  
Tissue Integration

Successful dental implants rely on stable osseointegration and soft-tissue integration. Titania nanotubes (TNTs) with a diameter of 100 nm could increase the mesenchymal stem cell response and simultaneously decrease Staphylococcus aureus adhesion. However, the interactions between the modified surface and surrounding soft tissues are still unknown. In the present study, we fully investigated the biological behavior of human gingival fibroblasts (HGFs) and the adhesion of Porphyromonas gingivalis (P. gingivalis). TNTs were synthesized on titanium (Ti) surfaces by electrochemical anodization at 10, 30, and 60 V, and the products were denoted as NT10, NT30, and NT60, respectively. NT10 (diameter: 30 nm) and NT30 (diameter: 100 nm) could enhance the HGF functions, such as cell attachment and proliferation and extracellular matrix- (ECM-) related gene expressions, with the latter showing higher enhancement. NT60 (diameter: 200 nm) clearly impaired cell adhesion and proliferation and ECM-related gene expressions. Bacterial adhesion on the TNTs decreased and reached the lowest value on NT30. Therefore, NT30 without pharmaceuticals can be used to substantially enhance the HGF response and reduce P. gingivalis adhesion to the utmost, thus demonstrating significant potential in the transgingival part of dental implants.

scholarly journals Titanium surface modifications and their soft-tissue interface on nonkeratinized soft tissues—A systematic review (Review)

2019 ◽  
Vol 14 (4) ◽  
pp. 040802 ◽  
Author(s):  
Brandaan G. R. Zigterman ◽  
Casper Van den Borre ◽  
Annabel Braem ◽  
Maurice Y. Mommaerts
Keyword(s):  
Systematic Review ◽  
Soft Tissue ◽  
Titanium Surface ◽  
Soft Tissues ◽  
Surface Modifications ◽  
Tissue Interface

scholarly journals Hydrofluoric acid treatment of titanium surfaces enhances the proliferation of human gingival fibroblasts

2019 ◽  
Vol 10 ◽  
pp. 204173141982895 ◽  
Author(s):  
Maria H Pham ◽  
Håvard J Haugen ◽  
Alessandra Rinna ◽  
Jan Eirik Ellingsen ◽  
Janne E Reseland
Keyword(s):  
Soft Tissue ◽  
Interleukin 6 ◽  
Hydrofluoric Acid ◽  
Fold Increase ◽  
Human Gingival Fibroblasts ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Implant Surface ◽  
The Media

The attachment of implants relies on bone and soft tissue biocompatibility. The aim of this article is to investigate the effect of fluoride-modified metallic titanium (Ti) surfaces (Ti-F) on proliferation and differentiation of human gingival fibroblasts. Human gingival fibroblast cells were exposed to hydrofluoric acid–modified Ti coins (Ti-F) for 1, 3, 7, 14 and 21 days, and untreated coins were used as controls. A five- to six-fold increase in the proliferation of human gingival fibroblasts on Ti-F compared to Ti surfaces was observed. Enhanced gene expression of interleukin-6 and osteoprotegerin was found at 7 days. Increased levels of sclerostin, interleukin-6 and osteoprotegerin in the media from human gingival fibroblasts cultured on Ti-F coins were found compared to controls. Our results confirm that hydrofluoric acid–modified surface may indirectly enhance the firm attachment of implant surface to junction epithelium, soft tissue epithelium, which would give protection for underlying osseous structures making osseointegration of the dental implant possible.

scholarly journals Anti-Inflammatory and Antibacterial Activity of the Chitosan/Chlorhexidine Gel Commercial Preparation for Postexodontia Treatment: An In Vitro Study

2020 ◽  
Vol 14 (03) ◽  
pp. 397-403
Author(s):  
Rafael Torres-Rosas ◽  
Nayely Torres-Gómez ◽  
Adriana Moreno-Rodríguez ◽  
René García-Contreras ◽  
Liliana Argueta-Figueroa
Keyword(s):  
Antibacterial Activity ◽  
Bone Cells ◽  
Oral Surgery ◽  
Raw 264.7 Cells ◽  
Human Gingival Fibroblast ◽  
Raw 264.7 ◽  
Gingival Fibroblast ◽  
Commercial Preparation ◽  
Topical Gel

Abstract Objective The present study aimed to assess in vitro the antibacterial activity, cytotoxicity, and the expression of prostaglandin E2 (PGE2) of Bexident post topical gel (BP). Materials and Methods The broth dilution test was performed to analyze the antimicrobial activity of BP against Staphylococcus aureus, Escherichia coli, and Streptococcus mutans. Minimal bactericidal concentrations (MBCs) and minimal inhibitory concentrations (MICs) were assessed. Cytotoxic activity was performed by the MTT (tetrazolium dye) method on human gingival fibroblast (HGF), human bone cells (HBC), and human pulp cells (HPC) (from primary cell culture) and HGF-1 from American Type Culture Collection. The expression of PGE2 produced by RAW 264.7 cells was determined by ELISA utilizing an Enzyme Immuno-Assay Kit. Statistical Analysis Shapiro–Wilks normality test and Mann–Whitney U test were performed for all data. Results The MBCs of BP for S. aureus, E. coli, and S. mutans were found at 25, 50, and 12.5%, respectively. The MICs for the same strains were found at 12.5, 25, and 3.125%. The CC50 of BP gel for HBC, HPC, and HGF, and HGF-1 were 12.5 ± 1.09, 0.37 ± 0.02, 0.35 ± 0.02, and 20.4 ± 0.02%, respectively. The levels of expression PGE2 produced by RAW 264.7 cells treated with IL-1β exhibit an inverse dose-dependent effect on the concentrations of BP gel used. Conclusion Our results indicate that the BP gel has a great antibacterial effect, adequate biocompatibility, showing a decrease in the expression of PGE2 on cells with previously induced inflammation. Due to the above, its use as a healing agent after oral surgery seems to be adequate.

scholarly journals Comparison of Resorbable Membranes for Guided Bone Regeneration of Human and Bovine Origin

2014 ◽  
Vol 64 (4) ◽  
pp. 477-492
Author(s):  
Lazić Zoran ◽  
Bubalo Marija ◽  
Milović Radomir ◽  
Matijević Stevo ◽  
Magić Marko ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Bone Regeneration ◽  
Soft Tissues ◽  
Alveolar Bone ◽  
Guided Bone Regeneration ◽  
Epithelial Migration ◽  
Tissue Integration ◽  
The Third ◽  
Tissue Proliferation ◽  
Space Reduction

Abstract The properties of membranes for guided bone regeneration have been described by a number of authors. These involve biocompatibility, appropriate barrier features (mechanical prevention of soft tissue proliferation), tissue integration, immunologic neutrality, preservation of the space for new alveolar bone, and simplicity of application. Such membrane must hold out against the masticatory forces and tissue tension of the fl ap, and prevent the collapse of soft tissues and wound space reduction. The property of integration into the tissue guarantees wound stabilization and inhibits epithelial migration. The aim of this study was to compare and evaluate the infl uence of human resorbable demineralized membrane (RHDM) and bovine resorbable demineralized membrane (RBDM) on bone regeneration. The experiment, approved by the Ethical Committee, was performed on six dogs and conducted into three phases. Bone defects were created in all six dogs on the left side of the mandible, 8 weeks after extraction of the second, third and fourth premolar. One defect was covered with 200 μm thick RHDM, one with 200 μm thick RBDM, and the third defect was left empty (control defect). The pathohistological analysis was done two, four and six months after the surgery. In the third phase samples of bone tissue were taken and subjected to patohistological analysis. In all six dogs the defects treated with RHDM 200μm thick showed a higher level of bone regeneration in comparison with the defect treated with RBDM 200 μm thick and especially with the control defect. Our results demonstrated that the human membrane showed the least soft tissue ingrowth and promoted better bone formation at 6 months compared with a bovine one.

scholarly journals Tuning Nanopore Diameter of Titanium Surfaces to Improve Human Gingival Fibroblast Response

2018 ◽  
Vol 19 (10) ◽  
pp. 2881 ◽  
Author(s):  
Maria Ferrà-Cañellas ◽  
Maria Llopis-Grimalt ◽  
Marta Monjo ◽  
Joana Ramis
Keyword(s):  
Cellular Response ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Nanostructured Surfaces ◽  
Force Microscopy ◽  
Interelectrode Spacing ◽  
Tissue Integration ◽  
Atomic Force ◽  
Titanium Surfaces ◽  
Different Diameters

The aim of this study was to determine the optimal nanopore diameter of titanium nanostructured surfaces to improve human gingival fibroblast (hGF) response, with the purpose of promoting gingiva integration to dental implant abutments. Two TiO2 nanoporous groups with different diameters (NP-S ~48 nm and NP-B ~74 nm) were grown on Ti foils using an organic electrolyte containing fluoride by electrochemical oxidation, varying the applied voltage and the interelectrode spacing. The surfaces were characterized by scanning electron microscope (SEM), atomic force microscopy (AFM), and contact angle. The hGF were cultured onto the different surfaces, and metabolic activity, cytotoxicity, cell adhesion, and gene expression were analyzed. Bigger porous diameters (NP-B) were obtained by increasing the voltage used during anodization. To obtain the smallest diameter (NP-S), apart from lowering the voltage, a lower interelectrode spacing was needed. The greatest surface area and number of peaks was found for NP-B, despite these samples not being the roughest as defined by Ra. NP-B had a better cellular response compared to NP-S. However, these effects had a significant dependence on the cell donor. In conclusion, nanoporous groups with a diameter in the range of 74 nm induce a better hGF response, which may be beneficial for an effective soft tissue integration around the implant.

scholarly journals Cytocompatibility of Titanium, Zirconia and Modified PEEK after Surface Treatment Using UV Light or Non-Thermal Plasma

2019 ◽  
Vol 20 (22) ◽  
pp. 5596 ◽  
Author(s):  
Linna Guo ◽  
Ralf Smeets ◽  
Lan Kluwe ◽  
Philip Hartjen ◽  
Mike Barbeck ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Dental Implants ◽  
Thermal Plasma ◽  
Oxygen Plasma ◽  
Uv Light ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Non Thermal Plasma ◽  
Tissue Cells

A number of modifications have been developed in order to enhance surface cytocompatibility for prosthetic support of dental implants. Among them, ultraviolet (UV) light and non-thermal plasma (NTP) treatment are promising methods. The objective of this study was to compare the effects of UV light and NTP on machined titanium, zirconia and modified polyetheretherketone (PEEK, BioHPP) surfaces in vitro. Machined samples of titanium, zirconia and BioHPP were treated by UV light and NTP of argon or oxygen for 12 min each. Non-treated disks were set as controls. A mouse fibroblast and a human gingival fibroblast cell line were used for in vitro experiments. After 2, 24 and 48 h of incubation, the attachment, viability and cytotoxicity of cells on surfaces were assessed. Results: Titanium, zirconia and BioHPP surfaces treated by UV light and oxygen plasma were more favorable to the early attachment of soft-tissue cells than non-treated surfaces, and the number of cells on those treated surfaces was significantly increased after 2, 24 and 48 h of incubation (p < 0.05). However, the effects of argon plasma treatment on the cytocompatibility of soft tissue cells varied with the type of cells and the treated material. UV light and oxygen plasma treatments may improve the attachment of fibroblast cells on machined titanium, zirconia and PEEK surfaces, that are materials for prosthetic support of dental implants.

scholarly journals Influence of Polysaccharides’ Molecular Structure on the Antibacterial Activity and Cytotoxicity of Green Synthesized Composites Based on Silver Nanoparticles and Carboxymethyl-Cellulose

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1164 ◽  
Author(s):  
María de los Ángeles Martínez-Rodríguez ◽  
Elizabeth Madla-Cruz ◽  
Victor H. Urrutia-Baca ◽  
Myriam A. de la Garza-Ramos ◽  
Virgilio A. González-González ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Aqueous Solutions ◽  
Carboxymethyl Cellulose ◽  
Aqueous Dispersion ◽  
Human Gingival Fibroblast ◽  
Spectroscopic Techniques ◽  
Gingival Fibroblast ◽  
Coordination Bonds

In this paper we report on the influence of polysaccharides’ molecular structure on the antibacterial activity and cytotoxicity of composites based on silver nanoparticles (AgNPs) immobilized into carboxymethyl-cellulose (CMC). These composites were green synthesized from the reduction of silver ions into aqueous solutions of the polysaccharide, using CMC with different degree of substitution (DS) and molecular weight (Mw). The composites were characterized by transmission electron microscopy (TEM), as well as infrared (ATR-FTIR), ultraviolet (UV-Vis), Raman, and X-ray photo-electron (XPS) spectroscopic techniques. The antibacterial activity was evaluated with minimum inhibitory concentration against Enterococcus faecalis. The cytotoxicity of composites was assessed against human gingival fibroblast. Experimental evidence suggests that particle size distribution and morphology of AgNPs change according to the quantity of silver precursor added to the reaction, as well as the DS and Mw of CMC used for composites preparation. This is related to the dispersion of silver precursor into aqueous solutions of the polysaccharide and the formation of Ag-O coordination bonds among AgNPs and COO− moieties of CMC. Moreover, these coordination bonds modify the ability of nanoparticles to produce and release Ag+ into aqueous dispersion, adjusting their antibacterial activity and the induction of cytotoxicity into the tested biological environments.

scholarly journals Study on Effects of Titanium Surface Microporous Coatings Containing Zinc on Osteoblast Adhesion and Its Antibacterial Activity

2017 ◽  
Vol 2017 ◽  
pp. 1-4
Author(s):  
Quan-ming Zhao ◽  
Guang-zhong Li ◽  
Hao-ming Zhu ◽  
Li Cheng
Keyword(s):  
Antibacterial Activity ◽  
Titanium Surface ◽  
Antibacterial Properties ◽  
Pure Titanium ◽  
Research Focus ◽  
Adhesion Ability ◽  
Good Antibacterial Activity ◽  
Osteoblast Adhesion ◽  
Function Integration

Metal surface structure/biomedical function integration is the current research focus. In previous studies, we have successfully prepared the microporous coatings containing zinc on the pure titanium surface by MAO. In the study, osteoblasts were seeded on the surface of the microporous coatings containing zinc and the adhesion of osteoblasts were evaluated, and the antibacterial activity of the microporous coatings containing zinc is observed through in vitro bacterial experiments. The result indicates that the adhesion ability of osteoblasts on the surface of microporous coatings containing zinc was very good, and the coatings could obviously inhibit the growth ofStaphylococcus aureusand had good antibacterial activity. In conclusion, the microporous coatings containing zinc on titanium surface have good osteogenic and antibacterial properties and have good application prospect.

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