scholarly journals Antimicrobial Activity and Chemical Composition of the Essential Oils of Portuguese Foeniculum vulgare Fruits

2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Ana S. Mota ◽  
M. Rosário Martins ◽  
Sílvia Arantes ◽  
Violeta R. Lopes ◽  
Eliseu Bettencourt ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Salmonella Enteritidis ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Foeniculum Vulgare ◽  
Serovar Typhimurium ◽  
Food Spoilage Fungi

The aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of Portugal. Composition of essential oils was established by Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The obtained yields of the essential oils were found to vary greatly in the range of 1.1 to 2.9% (v/w) and the chemical composition varied with the region of collection. A total of 16 compounds were identified. The main compounds were fenchone (16.9 – 34.7%), estragole (2.5 – 66.0%) and trans-anethole (7.9 – 77.7%). The percentages of these three main compounds were used to determine the relationship between the different oil samples and to group them into four different chemotypes: anethole/fenchone; anethole; estragole and anethole/estragole. Antifungal activity of essential oils was evaluated against six food spoilage fungi: Aspergillus niger, A. japonicus, A. oryzae, Fusarium oxysporum, Rhizophus oryzae and R. stolonifer. Antibacterial activity was assessed against three Gram-positive strains: Enterococcus faecalis ATCC 29212, Staphylococcus epidermidis ATCC 12228 and S. aureus ATCC 28213; and against six Gram-negative strains: Escherichia coli ATCC 25922; Morganella morganii LFG 08; Proteus mirabilis LFG 04; Salmonella enteritidis LFG 05; S. entiritidis serovar typhimurium LFG 06 and Pseudomonas aeruginosa ATCC 27853 by the disc diffusion agar method; the minimal inhibitory concentration (MIC) was determined using the broth macro-dilution method. The MIC values varied from 62.5 ( E. coli ATCC 25922) to 2000 μg/mL ( P. aeruginosa ATCC 27853).

scholarly journals Chemical composition and antimicrobial activity of berry essential oil of Juniperus oxycedrus L. (Cupressaceae) grown wild in Republic of Macedonia

2013 ◽  
Vol 59 ◽  
pp. 41-48 ◽  
Author(s):  
Floresha Sela ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokikj ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Salmonella Enteritidis ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Juniperus Oxycedrus ◽  
Republic Of Macedonia

Chemical composition and antimicrobial activity of essential oil isolated from berries from 2 different samples of Juniperus oxycedrus L. (Cupressaceae), growing wild in Republic of Macedonia was investigated. Performing GC/FID/MS analysis, one hundred components were identified, representing 96.0-98.95% of the oil. The major components were α-pinene (22.54- 27.12%), myrcene (11.26- 15.13%) and limonene (2.78-18.06%). Antimicrobial screening of the J. oxycedrus essential oils was made by disc diffusion and broth dilution method against 16 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. The most sensitive bacteria was Haemophilus influenzae (MIC = 125 ml/ml). The essential oils showed moderate antimicrobial activity against Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pyogenes, Corynebacterium spp., Escherichia coli and Campilobacter jejuni (MIC > 500 ml/ml) and no activity against Candida albicans, Staphylococcus epidermidis, Acinetobacter spp., Salmonella enteritidis, Shigella flexnery, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterococcus and Proteus mirabilis.

scholarly journals Correlation between Chemical Composition of Greek Essential Oils and their Antibacterial Activity against Food-borne Pathogens

2007 ◽  
Vol 2 (4) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Nikos G. Chorianopoulos ◽  
Epameinontas T. Evergetis ◽  
Nektarios Aligiannis ◽  
Sofia Mitakou ◽  
George-John E. Nychas ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Salmonella Enteritidis ◽  
Principal Component ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Food Borne

The compositions of forty-nine essential oils obtained from plants growing in areas of biodiversity in Greece were determined by means of gas chromatography and gas chromatography–mass spectrometry. The in vitro antibacterial activities of the oils were evaluated against a panel of five common food-borne bacteria ( Escherichia coli 0157:H7 NCTC 12900, Salmonella Enteritidis PT4, Staphylococcus aureus ATCC 6538, Listeria monocytogenes Scott A and Bacillus cereus FSS 134). Monoterpene hydrocarbons, phenolic monoterpenes and alcohols were the major constituents of the oils, but their concentrations varied greatly between the samples examined. The essential oils obtained from the species endemic to Greece ( Satureja and Origanum) had the most potent bactericidal properties. The chemical composition data were statistically processed through Principal Component Analysis (PCA) and correlated with the antibacterial activities, allowing a rough estimation of the potential of the essential oils antibacterial activity.

scholarly journals Essential Oil Composition ofPinus peuceGriseb. Needles and Twigs from Two National Parks of Kosovo

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Avni Hajdari ◽  
Behxhet Mustafa ◽  
Dashnor Nebija ◽  
Hyrmete Selimi ◽  
Zeqir Veselaj ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
National Park ◽  
Essential Oil Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Plant Materials ◽  
Geographical Variations

The principal aim of this study was to analyze the chemical composition and qualitative and quantitative variability of essential oils obtained from seven naturally grown populations of thePinus peuceGrisebach, Pinaceae in Kosovo. Plant materials were collected from three populations in the Sharri National Park and from four other populations in the Bjeshkët e Nemuna National Park, in Kosovo. Essential oils were obtained by steam distillation and analyzed by GC-FID (Gas Chromatography-Flame Ionization Detection) and GC-MS (Gas Chromatography-Mass Spectrometry). The results showed that the yield of essential oils (v/wdry weight) varied depending on the origin of population and the plant organs and ranged from 0.7 to 3.3%. In total, 51 compounds were identified. The main compounds wereα-pinene (needles: 21.6–34.9%; twigs: 11.0–24%),β-phellandrene (needles: 4.1–27.7; twigs: 29.0–49.8%), andβ-pinene (needles: 10.0–16.1; twigs: 6.9–20.7%). HCA (Hierarchical Cluster Analysis) and PCA (Principal Component Analyses) were used to assess geographical variations in essential oil composition. Statistical analysis showed that the analyzed populations are grouped in three main clusters which seem to reflect microclimatic conditions on the chemical composition of the essential oils.

scholarly journals Comparison of the Chemical Composition and Antimicrobial Activity of Thymus serpyllum Essential Oils

2015 ◽  
Vol 43 (2) ◽  
pp. 432-438 ◽  
Author(s):  
Aneta WESOŁOWSKA ◽  
Monika GRZESZCZUK ◽  
Dorota JADCZAK ◽  
Paweł NAWROTEK ◽  
Magdalena STRUK
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Gas Chromatography Mass Spectrometry ◽  
E Coli ◽  
Thymus Serpyllum ◽  
Main Components ◽  
Total Oil

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.

scholarly journals The First Report on Chemical Composition and Antimicrobial Activity of Artemisia scoparia Waldst. et Kit. Extracts

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091503 ◽  
Author(s):  
Gordana S. Stojanović ◽  
Jovana D. Ickovski ◽  
Aleksandra S. Đorđević ◽  
Goran M. Petrović ◽  
Katarina D. Stepić ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Volatile Components ◽  
Bacterial Strains ◽  
First Report ◽  
Main Compound ◽  
Artemisia Scoparia

Volatiles of diethyl ether extract (DE), ethyl acetate extract (EE), and hexane extract (HE) of Artemisia scoparia Waldst. et Kit. were analyzed by gas chromatography with flame ionization detector and gas chromatography-mass spectrometry. In both DE and EE, the main compound was scoparone (24.0% and 86.1%, respectively) while in the HE, alkanes were dominant with nonacosane as the most represented (19.4%). Antimicrobial activity was tested against 4 bacterial strains and 1 fungal strain using disc-diffusion method. Tested samples were inactive against Gram-negative bacteria and they exhibited activity against Gram-positive bacteria and yeast Candida albicans. This is the first report on the chemical composition of volatile components and antimicrobial activity of DE, EE, and HE of A. scoparia Waldst. et Kit.

scholarly journals Effect of the Chemical Composition of Free-Terpene Hydrocarbons Essential Oils on Antifungal Activity

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3532
Author(s):  
Ben Salha ◽  
Herrera Díaz ◽  
Lengliz ◽  
Abderrabba ◽  
Labidi
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pharmaceutical Products ◽  
Gas Chromatography Mass Spectrometry ◽  
Reduced Pressure ◽  
Oxygenated Compounds ◽  
Food Spoilage Fungi

In this study, Carum carvi L. essential oil (CEO) and Origanum majorana L. essential oil (MEO) was steam-distillated under reduced pressure. We henceforth obtained three fractions for each essential oil: CF1, CF2, CF3, MF1, MF2, and MF3. Then, these fractions were characterized using the gas chromatography–mass spectrometry (GC-MS) technique. The results indicated that some fractions were rich in oxygenated compounds (i.e., CF2, CF3, MF2, and MF3) with concentrations ranging from 79.21% to 98.56%. Therefore, the influence of the chemical composition of the essential oils on their antifungal activity was studied. For this purpose, three food spoilage fungi were isolated, identified, and inoculated in vitro, in order to measure the antifungal activity of CEO, MEO, and their fractions. The results showed that stronger fungi growth inhibitions (FGI) (above 95%) were found in fractions with higher percentages of oxygenated compounds, especially with (−)-carvone and terpin-4-ol as the major components. Firstly, this work reveals that the free-terpenes hydrocarbons fractions obtained from MEO present higher antifungal activity than the raw essential oil against two families of fungi. Then, it suggests that the isolation of (−)-carvone (97.15 ± 5.97%) from CEO via vacuum distillation can be employed successfully to improve antifungal activity by killing fungi (FGI = 100%). This study highlights that separation under reduced pressure is a simple green method to obtain fractions or to isolate compounds with higher biological activity useful for pharmaceutical products or natural additives in formulations.

Chemical composition and biological activities of leaf and fruit essential oils from Eucalyptus camaldulensis

2017 ◽  
Vol 72 (11-12) ◽  
pp. 483-489 ◽  
Author(s):  
Gulden Dogan ◽  
Nazan Kara ◽  
Eyup Bagci ◽  
Seher Gur
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oils ◽  
Biological Activities ◽  
Eucalyptus Camaldulensis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Leaf Oil ◽  
Fruit Oil ◽  
Antibacterial And Antifungal

AbstractThe chemical composition of the essential oils from the leaves and fruit ofEucalyptus camaldulensisgrown in Mersin, Turkey was analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) techniques. The biological activities (antibacterial and antifungal) were examined using the agar well diffusion method. The main leaf oil constituents werep-cymene (42.1%), eucalyptol (1,8-cineole) (14.1%), α-pinene (12.7%) and α-terpinol (10.7%). The main constituents of the fruit oil were eucalyptol (1,8-cineole) (34.5%),p-cymene (30.0%), α-terpinol (15.1%) and α-pinene (9.0%). Our results showed that both types of oils are rich in terms of monoterpene hydrocarbons and oxygenated monoterpenes. The leaf and fruit essential oils ofE. camaldulensissignificantly inhibited the growth of Gram-positive (Staphylococcus aureusandBacillus subtilis) and Gram-negative (Escherichia coliandStreptococcussp.) bacteria (p<0.05). The oils also showed fungicidal activity againstCandida tropicalisandC. globrata. Leaf essential oils showed more activity than fruit essential oils, probably due to the higher p-cymene concentration in leaves.

scholarly journals Antimicrobial Activity and Analysis of the Essential Oils of Selected Endemic Edible Apiaceae Plants Root from Caspian Hyrcanian Region (North of Iran)

2019 ◽  
Vol 25 (2) ◽  
pp. 138-144
Author(s):  
Azadeh Hamedi ◽  
Ardalan Pasdaran ◽  
Arsalan Pasdaran
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Fatty Acid Esters ◽  
Herbal Remedies ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Chemical Compositions ◽  
Root Essential Oil

Background: Different endemic species of Apiaceae that widely grow in Hyrcanian region have long been used as spices and herbal remedies. Chemical compositions and bioactivity of many of these plants have not been studied scientifically. The volatile constituents and antimicrobial activity of four edible Apiaceae plants—Elaeosticta glaucescens Boiss, Malabaila secacul (Mill.) Boiss, Caucalis platycarpos L., and Eryngium caucasicum Trautv—roots were investigated. Methods: Gas chromatography/mass spectrometry (GC–MS) and gas chromatography/flame ionization detection (GC–FID) methods were used for chemical investigation. Antimicrobial potential of the volatile compositions of these roots were investigated using the disc diffusion method on four microorganisms including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis. Results: The best minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these species were revealed for B. subtilis and S. aureus (500 µg/mL for MIC and MBC) followed by E. caucasicum and C. platycarpus, respectively. The main compounds of E. caucasicum root essential oil were hexyl isovalerate (11.0%) and hexyl valerate (10.1%). E. glaucescens root essential oil was mainly composed of 3(10)-caren-4-ol, acetoacetic acid ester (9.8%), octyl isovalerate (8.9%), and octyl acetate (5.7%). In C. platycarpos root, hexyl isovalerate (9.2%) and n-octyl isobutyrate (8.5%) were constituted the major compositions of the essential oil while n-hexyl isobutyrate (8.5%) and hexyl hexanoate (7.8%) were predominant compounds in M. secacul. Conclusion: The essential oils of E. caucasicum and C. platycarpus roots were contained high amount of short chain fatty acid esters (SCFAE) and trans- pinocarvyl acetate. They exhibited moderate antibacterial activity.

scholarly journals CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OILS OF FOUR VARIETIES OF LIPPIA MULTIFLORA IN BENIN

2019 ◽  
Author(s):  
GANDONOU Dossa Clément ◽  
BAMBOLA Bouraïma ◽  
TOUKOUROU Habib ◽  
GBAGUIDI Ahokannou Fernand ◽  
DANSOU Christian ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Diffusion Method ◽  
Lippia Multiflora ◽  
Natural Substances

Present study involves the study of the chemical composition of the essential oils extracted from the leaves by gas chromatography and gas chromatography coupled with mass spectrometry of Lippia multiflora harvested in the regions of Kétou, Savalou, Bohicon and Mono and tested by the well diffusion method against pathogenic microorganisms. The essential oils studied are terpene compounds, aromatic compounds, aliphatic compounds and other natural substances. The inhibition zone diameters determined allowed us to evaluate their degree of germ sensitivity of the strains tested. Essential oils extracted from Lippia multiflora harvested in these areas have the most pronounced antimicrobial activity. In total, the essential oils tested have different and specifically a degree of sensitivity against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Proteus mirabilis A24974, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa except that harvested in the Savalou who does not have no degree of sensitivity on Pseudomonas aeruginosa. This work paves the way for food preservation with extracted natural substances and also the formulation of natural antimicrobials for this fact.

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