scholarly journals Hypotensive Activity of Ethanolic Extracts of Morinda citrifolia L. Leaves and Fruit in Dexamethasone-Induced Hypertensive Rat

2016 ◽  
Vol 22 (1) ◽  
pp. 107-113 ◽  
Author(s):  
Dyan Wigati ◽  
Khoerul Anwar ◽  
Sudarsono ◽  
Agung Endro Nugroho
Keyword(s):  
Blood Pressure ◽  
Renal Damage ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Morinda Citrifolia ◽  
Combination Group ◽  
Total Phenolic ◽  
Hypotensive Activity ◽  
Arterial Blood ◽  
Induced Hypertension

The effect of ethanolic extract of Morinda citrifolia leaves and fruit on blood pressure in dexamethasone-induced hypertension rat was evaluated. Total phenolic content of Morinda citrifolia leaves ethanolic extract (MCLEE) and Morinda citrifolia leaves ethanolic extract (MCFEE) was 1.789 ± 0.116 and 1.677 ± 0.051 mg of gallic acid equivalents per gram sample, respectively. Rutin level in MCLEE was 0.92 ± 0.19%, and scopoletin level in MCFEE was 0.46 ± 0.05%. MCLEE, MCFEE, and its extract combination significantly decreased the blood pressure of hypertensive rats. The combination group showed highest hypotensive activity by lowering systolic blood pressure by 16.71 ± 3.95%, diastolic blood pressure by 21.49 ± 7.90%, and mean arterial blood pressure by 19.58% ± 6.35. All extract treatments have not been able to repair or inhibit renal damage caused by dexamethasone induction.

scholarly journals Increased superoxide levels in ganglia and sympathoexcitation are involved in sarafotoxin 6c-induced hypertension

2008 ◽  
Vol 295 (5) ◽  
pp. R1546-R1554 ◽  
Author(s):  
Melissa Li ◽  
Xiaoling Dai ◽  
Stephanie Watts ◽  
David Kreulen ◽  
Gregory Fink
Keyword(s):  
Blood Pressure ◽  
Sympathetic Innervation ◽  
Sympathetic Ganglia ◽  
Plasma Norepinephrine ◽  
Sprague Dawley ◽  
Surgical Ablation ◽  
Arterial Blood ◽  
Sprague Dawley Rats ◽  
Induced Hypertension ◽  
Hypertension Development

Endothelin (ET) type B receptors (ETBR) are expressed in multiple tissues and perform different functions depending on their location. ETBR mediate endothelium-dependent vasodilation, clearance of circulating ET, and diuretic effects; all of these should produce a fall in arterial blood pressure. However, we recently showed that chronic activation of ETBR in rats with the selective agonist sarafotoxin 6c (S6c) causes sustained hypertension. We have proposed that one mechanism of this effect is constriction of capacitance vessels. The current study was performed to determine whether S6c hypertension is caused by increased generation of reactive oxygen species (ROS) and/or activation of the sympathetic nervous system. The model used was continuous 5-day infusion of S6c into male Sprague-Dawley rats. No changes in superoxide anion levels in arteries and veins were found in hypertensive S6c-treated rats. However, superoxide levels were increased in sympathetic ganglia from S6c-treated rats. In addition, superoxide levels in ganglia increased progressively the longer the animals received S6c. Treatment with the antioxidant tempol impaired S6c-induced hypertension and decreased superoxide levels in ganglia. Acute ganglion blockade lowered blood pressure more in S6c-treated rats than in vehicle-treated rats. Although plasma norepinephrine levels were not increased in S6c hypertension, surgical ablation of the celiac ganglion plexus, which provides most of the sympathetic innervation to the splanchnic organs, significantly attenuated hypertension development. The results suggest that S6c-induced hypertension is partially mediated by sympathoexcitation to the splanchnic organs driven by increased oxidative stress in prevertebral sympathetic ganglia.

scholarly journals Resistance Training Controls Arterial Blood Pressure in Rats with L-NAME- Induced Hypertension

2013 ◽  
Author(s):  
Ayslan Jorge Santos de Araujo ◽  
Anne Carolline Veríssimo dos Santos ◽  
Karine dos Santos Souza ◽  
Marlúcia Bastos Aires ◽  
Valter Joviniano Santana-Filho ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Resistance Training ◽  
Arterial Blood Pressure ◽  
Arterial Blood ◽  
Induced Hypertension

scholarly journals Polyphenols in Coreopsis tinctoria Nutt. fruits and the plant extracts antioxidant capacity evaluation

2014 ◽  
Vol 12 (8) ◽  
pp. 858-867 ◽  
Author(s):  
Christina Zălaru ◽  
Claudia Crişan ◽  
Ioan Călinescu ◽  
Zenovia Moldovan ◽  
Isabela Ţârcomnicu ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Plant Extracts ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Optimum Operating ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Coreopsis Tinctoria

AbstractThe aim of this research was to investigate the chemical composition of Coreopsis tinctoria Nutt. fruits extract, to highlight the potential of ultrasound assisted extraction in the fast preparation of extracts rich in polyphenols using different solvents (55%, 78% and 96% hydrous ethanol) and to evaluate the antioxidant potential of formulated extracts. LC-MS/MS was used to characterize the ethanolic extract from Coreopsis tinctoria Nutt. dried fruits. The extract contains different flavonoids (marein, flavanomarein, quercetagetin-7-O-glucoside, okanin aurone, leptosidin, luteolin, apigenin) and phenolic acids (chlorogenic acid, caffeic acid). Several parameters that could affect extraction efficiency were evaluated. Finally, this study focused on determination of plant extracts total phenolic content and their antioxidant capacity. The experimental results allowed the selection of the optimum operating parameters leading to the highest total polyphenolic content, expressed as gallic acid equivalents, and avoiding the degradation of phenolic compounds (ethanol 55%; extraction temperature 323.15 K, extraction time 30 min, liquid/solid ratio 20/1). A good relationship between total phenolic content and antioxidant capacity was obtained.

scholarly journals Allablanckia floribunda hypotensive activity on ethanol induced hypertension in rats

2018 ◽  
Vol 7 (2) ◽  
pp. 146-151
Author(s):  
Danielle Claude Bilanda ◽  
◽  
Paul Désiré Djomeni Dzeufiet ◽  
Orelien Mtopi Bopda ◽  
Pierre Kamtchouing ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Aqueous Extract ◽  
Plant Extract ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Hdl Cholesterol ◽  
Hypotensive Activity ◽  
Hypertensive Rats ◽  
Induced Hypertension

Background: Chronic alcohol intake is related to hypertension. In the present work, we investigated the effect of Allablanckia floribunda Oliver (Clusiaceae) aqueous extract in alcohol-induced hypertensive rats and on related oxidative stress damages. Methods: Alcohol-induced hypertensive rats (AHR) was obtained by oral administration of ethanol (3 g/kg/day during 8 weeks). Blood pressure and heart rate were evaluated using the direct cannulation method. The effects of the extract on lipid profile as well as kidney and liver functions were studied. Free radical scavenging and antioxidant properties of the extract were evaluated by colorimetric method. The effects of A. floribunda were evaluated after 4 weeks of treatment with alcohol. Results: At the doses of 200 and 400 mg/kg/day, A. floribunda significantly decreased the mean blood pressure of AHR by 14.06 and 23.25 % respectively. Administration of the plant extract lead to the reduction of total cholesterol by 41.50% and 43.06%, HDL-cholesterol by 22.16 and 30.15% and artherogenic index by 69.78 and 74.43%, respectively at the doses of 200 and 400 mg/kg, as compared to untreated hypertensive rats. A. floribunda (200 and 400 mg/kg) decrease bilirubine (12.98 and 16.88%), urea (23.32% and 32.26 %), ALT (10.73 and 27.97%) and AST (29.80 and 42.22%) of treated AHR, respectively. The plant extract also reduced superoxide dismutase (SOD), malondialdehyde (MDA) and catalase and increased the reduced glutathione (GSH) concentration in aorta, heart, kidney and liver of AHR. Conclusion: These results suggest that the aqueous extract of A. floribunda possesses antioxidant and hypotensive activity in alcohol-induced hypertension

scholarly journals EVALUATION OF DEHYDRATED MUKIA MADERASPATANA LEAVES AS A POTENT SOURCE OF DIETARY ANTIOXIDANTS

2019 ◽  
Vol 7 (6) ◽  
pp. 27-36
Author(s):  
D. Amirtham ◽  
J. Aswini Nivedida ◽  
K. Dhivya ◽  
S. Ganapathy ◽  
C. Indurani
Keyword(s):  
Phenolic Content ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Antioxidant Property ◽  
Leafy Vegetables ◽  
Total Phenolic ◽  
Green Leafy Vegetables ◽  
Total Antioxidant ◽  
Indigenous Plant

Green leafy vegetables are the most underexploited class of vegetables despite high nutritional value. The current study has been focused on the evaluation of anti-oxidant status of fresh and dehydrated under- utilized green leafy vegetable namely Mukia maderaspatana (L.) (Family: Cucurbitaceae), an indigenous plant; traditionally it is used as an ingredient of various cocktail preparations for the management of severe inflammatory disorders in Indian system of medicine. The total phenolic content (TPC), total flavonoid content (TFC) and total antioxidant activities were evaluated for the ethanolic extract of leaves to assess the in vitro antioxidant activities. The results showed that there exist a linear correlation between polyphenol content and antioxidant property. The ethanolic extract of dehydrated Mukia leaves showed the highest phenolic content (269.34 ± 0.78mg GAE/g), and total antioxidant activity (543±46 µmol Trolox/100 g). HPTLC analysis has revealed the presence of significant quantity of Quercitin (26.52%), an important flavonoid of tremendous antioxidant, anticancer and ant inflammatory properties in both the fresh and dehydrated leaves which might be the chief bioactive principle in Mukia.

Antimicrobial, Antioxidant and Antihemolytic Activities of Two Daphne gnidium Leaves Extracts

2021 ◽  
Author(s):  
F. Kaabour ◽  
S. Mezaache-Aichour ◽  
K. Aissat
Keyword(s):  
Phenolic Content ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Oxidative Hemolysis ◽  
Scavenging Effect ◽  
Daphne Gnidium ◽  
Antibacterial And Antifungal ◽  
Β Carotene

In this study, Daphne gnidium leaves extracts were screened for their Phenolic content, antimicrobial, antioxidant and antihemolitic activities. Decoction and maceration were used to obtain the aqueous (Aq E) and the ethanolic extract (Et E), respectively. Total phenolic content was determined using Folin–Ciocalteu reagent. Moreover, a disc diffusion assay and inhibition of mycelial growth test were applied to evaluate the antibacterial and antifungal activity. The extracts were also tested for their antioxidant effects in terms of DPPH, OH, H2O2, β-carotene, and ferrous ion chelating assays. To investigate antihemolytic activity, the 2,2,-azobis (2-amidinopropane) dihydrochloride (AAPH) was used to induce erythrocyte oxidative hemolysis. Results indicated that ethanolic extract (Et E) contains the highest polyphenol content (775.25 μg GAE/mg extract). The Et E inhibited the growth of bacterial strains with inhibition zone diameters from 7 to 15 mm. In contrast, no activities have been found against all fungal strains. In antihemolytic test, aqueous and ethanolic extracts showed almost the same effect with an HT50 value of 106 min. Ethanolic extract was found to be more active in DPPH and β-carotene assays (IC50: 5.76 μg/ml and 70% inhibition respectively). However, the aqueous extract showed a greater effect than the ethanolic one in metal chelating activity assay (IC50: 170 μg/ml), OH scavenging effect assay (IC50: 9.67 μg/ml) and H2O2 scavenging assay (IC50: 133.2 μg/ml).

Phytochemical Evaluation and In-vitro Antioxidant Potential of whole plant of Caralluma adscendens

2021 ◽  
pp. 2774-2778
Author(s):  
Divya Yada ◽  
T. Sivakkumar ◽  
M. Sudhakar
Keyword(s):  
Nitric Oxide ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Whole Plant

The current line of investigation was focused at perusing the presence of phytochemical constituents, investigation of total phenol and flavonoid content, the antioxidant potential of various extracts of Caralluma adscendens whole plant using various in-vitro assays. The dried plant powder was extracted with various solvents based on polarity (Pet ether, Chloroform, Ethyl acetate, Ethanol and Aqueous) by hot continuous extraction in Soxhlet's apparatus and Extracts were dried. Phytoconstituents present in each extract was examined by performing preliminary phytochemical screening. Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and Antioxidant potential for crude extracts were studied by DPPH, nitric oxide scavenging and FRAP methods. The total phenolic content and flavonoid content of Ethanolic extract of plant was found to be 80.08±0.629mg and 70.88±1.170mg of GAE and Quercetin equivalents respectively. The Ethanolic extract exhibited potent antioxidant activity as determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH), nitric oxide scavenging and ferric reducing antioxidant power assays(FRAP) than the other extracts. The IC50 values for the Ethanolic extract of Caralluma adscendens was found to be 214.765±0.224 µg/ml and 215.928±0.506µg/ml by DPPH and nitric oxide scavenging assays respectively.

Abnormalities of peripheral venous tone in women with pregnancy-induced hypertension

1986 ◽  
Vol 70 (2) ◽  
pp. 155-157 ◽  
Author(s):  
Karen Stainer ◽  
Rachel Morrison ◽  
C. Pickles ◽  
A. J. Cowley
Keyword(s):  
Blood Pressure ◽  
Pregnant Women ◽  
Inverse Correlation ◽  
The Other ◽  
Normal Blood ◽  
Pregnancy Induced Hypertension ◽  
Normal Blood Pressure ◽  
Venous Tone ◽  
Arterial Blood ◽  
Induced Hypertension

1. Forearm venous tone was measured in two groups of pregnant women: one group with pregnancy-induced hypertension and the other group with normal blood pressure. 2. The women with pregnancy-induced hypertension were venoconstricted in the forearm (P < 0.01) compared with the pregnant women with normal blood pressure. However, there was no difference in venous tone between the women with pregnancy-induced hypertension and nonpregnant women. 3. There was an inverse correlation between mean arterial blood pressure and forearm venous tone (r = −0.581, P < 0.001) for all the pregnant women studied. Further evaluation of peripheral venous tone may provide valuable information about the pathophysiology and treatment of women with pregnancy-induced hypertension.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND GC-MS ANALYSIS OF THE ETHANOLIC EXTRACTS OF TERMINALIA BELLERICA ROXB (BAHEDA)

2016 ◽  
Vol 8 (11) ◽  
pp. 275
Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Neeraja Dwivedi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Terminalia Bellerica ◽  
Ms Analysis ◽  
Phytochemical Constituents

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.

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