scholarly journals Mechanisms of Growth Control of Kaposi's Sarcoma–Associated Herpes Virus–Associated Primary Effusion Lymphoma Cells

Blood ◽  
1998 ◽  
Vol 91 (7) ◽  
pp. 2475-2481 ◽  
Author(s):  
Hiroya Asou ◽  
Jonathan W. Said ◽  
Rong Yang ◽  
Reinhold Munker ◽  
Dorothy J. Park ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Lines ◽  
Antisense Oligonucleotides ◽  
Clonal Growth ◽  
Kaposi's Sarcoma ◽  
Herpes Virus ◽  
Primary Effusion Lymphoma ◽  
Kaposi’S Sarcoma ◽  
Gm Csf ◽  
Herpès Virus

Primary effusion lymphoma (PEL) is a distinct clinicopathologic entity associated with Kaposi's sarcoma-associated herpes virus (KSHV). Several cytokines, including interleukin-6 (IL-6), basic fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) may be important for survival of KS cells. However, little is known about the interaction of cytokines with KSHV-infected lymphocytes from PEL. Therefore, we investigated what cytokines were produced by KSHV-infected PEL cell lines (KS-1, BC-1, BC-2), what cytokine receptors were expressed by these cells, what response these cells had to selected cytokines, and what was the effect of IL-6 antisense phosphorothioated oligonucleotides. Reverse transcriptase-polymerase chain reaction (RT-PCR) and protein studies showed that these three cell lines produced IL-10, IL-6, and the receptors for IL-6. The granulocyte macrophage colony-stimulating factor (GM-CSF), IL-1β, IL-8, IL-12, bFGF, PDGF, and c-kit transcripts were not detected in the cell lines. High levels (0.7 to 5 ng/mL/106cells/48 hours) of IL-6 protein were consistently detected in supernatants of the cell lines by enzyme-linked immunosorbent assay (ELISA) tests. In clonogenic assays, interferon-α (IFN-α) and IFN-γ suppressed the clonal growth of the PEL cells, but GM-CSF, IL-4, IL-6, IL-8, IL-10, and oncostatin M did not change it. We examined for several autocrine loops that have been suggested to occur in KS. Experiments using antisense oligonucleotides showed that the clonal growth of KS-1 and BC-1 was nearly 100% inhibited by IL-6 antisense oligonucleotides (10 μmol/L), but not at all by either oligonucleotides (≤10 μmol/L) to IL-6 sense, IL-6 scrambled, viral IL-6 (vIL-6) antisense, or IL-10 antisense. Furthermore, the IL-6 antisense oligonucleotides had no effect on two B-cell lymphoma cell lines, which were not infected with KSHV. Addition of IL-6 antibody did not inhibit clonal growth of any of the cell lines. Taken together, we have defined the cytokines and their receptors expressed on PEL cells and have found that these cells synthesized IL-6 and IL-6 receptors; interruption of this pathway by IL-6 antisense oligonucleotides specifically prevented the growth of these cells. These findings will offer potential new therapeutic strategies for PEL.

scholarly journals Mechanisms of Growth Control of Kaposi's Sarcoma–Associated Herpes Virus–Associated Primary Effusion Lymphoma Cells

Blood ◽  
1998 ◽  
Vol 91 (7) ◽  
pp. 2475-2481 ◽  
Author(s):  
Hiroya Asou ◽  
Jonathan W. Said ◽  
Rong Yang ◽  
Reinhold Munker ◽  
Dorothy J. Park ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Lines ◽  
Antisense Oligonucleotides ◽  
Clonal Growth ◽  
Kaposi's Sarcoma ◽  
Herpes Virus ◽  
Primary Effusion Lymphoma ◽  
Kaposi’S Sarcoma ◽  
Gm Csf ◽  
Herpès Virus

Abstract Primary effusion lymphoma (PEL) is a distinct clinicopathologic entity associated with Kaposi's sarcoma-associated herpes virus (KSHV). Several cytokines, including interleukin-6 (IL-6), basic fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) may be important for survival of KS cells. However, little is known about the interaction of cytokines with KSHV-infected lymphocytes from PEL. Therefore, we investigated what cytokines were produced by KSHV-infected PEL cell lines (KS-1, BC-1, BC-2), what cytokine receptors were expressed by these cells, what response these cells had to selected cytokines, and what was the effect of IL-6 antisense phosphorothioated oligonucleotides. Reverse transcriptase-polymerase chain reaction (RT-PCR) and protein studies showed that these three cell lines produced IL-10, IL-6, and the receptors for IL-6. The granulocyte macrophage colony-stimulating factor (GM-CSF), IL-1β, IL-8, IL-12, bFGF, PDGF, and c-kit transcripts were not detected in the cell lines. High levels (0.7 to 5 ng/mL/106cells/48 hours) of IL-6 protein were consistently detected in supernatants of the cell lines by enzyme-linked immunosorbent assay (ELISA) tests. In clonogenic assays, interferon-α (IFN-α) and IFN-γ suppressed the clonal growth of the PEL cells, but GM-CSF, IL-4, IL-6, IL-8, IL-10, and oncostatin M did not change it. We examined for several autocrine loops that have been suggested to occur in KS. Experiments using antisense oligonucleotides showed that the clonal growth of KS-1 and BC-1 was nearly 100% inhibited by IL-6 antisense oligonucleotides (10 μmol/L), but not at all by either oligonucleotides (≤10 μmol/L) to IL-6 sense, IL-6 scrambled, viral IL-6 (vIL-6) antisense, or IL-10 antisense. Furthermore, the IL-6 antisense oligonucleotides had no effect on two B-cell lymphoma cell lines, which were not infected with KSHV. Addition of IL-6 antibody did not inhibit clonal growth of any of the cell lines. Taken together, we have defined the cytokines and their receptors expressed on PEL cells and have found that these cells synthesized IL-6 and IL-6 receptors; interruption of this pathway by IL-6 antisense oligonucleotides specifically prevented the growth of these cells. These findings will offer potential new therapeutic strategies for PEL.

Blood Dendritic Cells From Myeloma Patients Are Not Infected With Kaposi's Sarcoma-Associated Herpesvirus (KSHV/HHV-8)

Blood ◽  
1998 ◽  
Vol 92 (2) ◽  
pp. 402-404 ◽  
Author(s):  
Qing Yi ◽  
Marianne Ekman ◽  
Doina Anton ◽  
Susanne Bergenbrant ◽  
Anders Österborg ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Cell Lines ◽  
Kaposi's Sarcoma ◽  
Herpes Virus ◽  
Bone Marrow Cells ◽  
Kaposi’S Sarcoma ◽  
Myeloma Cell ◽  
Herpès Virus ◽  
Marrow Cells

In recent studies, the sequence of Kaposi's sarcoma-associated herpes virus (KSHV) or human herpes virus-8 (HHV-8) was detected in dendritic cells (DC) of patients with multiple myeloma (MM). A concern was raised whether there is an causal association between the viral infection and development of these tumors. In the present study, we have examined DC generated from blood adherent cells from 8 Swedish MM patients at different clinical stages and 2 patients with monoclonal gammopathy of undetermined significance. In addition, 6 myeloma cell lines and bone marrow cells from 2 MM patients were also studied. By polymerase chain reaction (PCR), including nested PCR, no virus DNA was demonstrable in the patients' DC or in myeloma cell lines or fresh bone marrow cells. Moreover, no antibody against KSHV was found in the serum of these 10 patients. Thus, our results indicate that blood-derived DC of MM patients in Sweden usually are not infected with KSHV/HHV-8. This study also suggests that KSHV/HHV-8 is not regularly associated with MM and consequently does not play a primary role in the pathogenesis of these tumors.

Involvement of Interleukin-10 (IL-10) and Viral IL-6 in the Spontaneous Growth of Kaposi’s Sarcoma Herpesvirus-Associated Infected Primary Effusion Lymphoma Cells

Blood ◽  
1999 ◽  
Vol 94 (8) ◽  
pp. 2871-2879 ◽  
Author(s):  
Karen D. Jones ◽  
Yoshiyasu Aoki ◽  
Yuan Chang ◽  
Patrick S. Moore ◽  
Robert Yarchoan ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Cell Lines ◽  
Neutralizing Antibodies ◽  
Kaposi's Sarcoma ◽  
Primary Effusion Lymphoma ◽  
Kaposi’S Sarcoma ◽  
Distinct Type ◽  
Interleukin 10 ◽  
Autocrine Growth

Abstract Primary effusion lymphoma (PEL) is a distinct type of lymphoma associated with Kaposi’s sarcoma-associated herpesvirus (KSHV) infection. To determine the factors responsible for the unrestrained proliferation of PEL, we have studied the growth factor requirements of the PEL-derived BCBL-1 and BC-1 cell lines. Both cell lines were found to be autocrine growth factor dependent and to release human interleukin-6 (IL-6), viral IL-6 (vIL-6), and human IL-10 in the culture supernatant. To establish whether these cytokines contribute to autocrine growth, neutralizing antibodies against human IL-6, vIL-6, human IL-10, and soluble IL-10 receptor were used. These experiments showed that human IL-10 and, to a lesser degree, vIL-6 serve as autocrine growth factors for BCBL-1 and BC-1 cells. Thus, human IL-10 and vIL-6 are growth factors released and used by PEL cells for autonomous proliferation and may be critical to the development and progression of PEL.

Multi-targeted therapy of everolimus in Kaposi’s sarcoma associated herpes virus infected primary effusion lymphoma

APOPTOSIS ◽  
2017 ◽  
Vol 22 (9) ◽  
pp. 1098-1115 ◽  
Author(s):  
Suchitra Mohanty ◽  
Amit Kumar ◽  
Piyanki Das ◽  
Sushil Kumar Sahu ◽  
Tathagata Choudhuri
Keyword(s):  
Targeted Therapy ◽  
Kaposi's Sarcoma ◽  
Herpes Virus ◽  
Primary Effusion Lymphoma ◽  
Kaposi’S Sarcoma ◽  
Herpès Virus

Blood Dendritic Cells From Myeloma Patients Are Not Infected With Kaposi's Sarcoma-Associated Herpesvirus (KSHV/HHV-8)

Blood ◽  
1998 ◽  
Vol 92 (2) ◽  
pp. 402-404 ◽  
Author(s):  
Qing Yi ◽  
Marianne Ekman ◽  
Doina Anton ◽  
Susanne Bergenbrant ◽  
Anders Österborg ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Cell Lines ◽  
Kaposi's Sarcoma ◽  
Herpes Virus ◽  
Bone Marrow Cells ◽  
Kaposi’S Sarcoma ◽  
Myeloma Cell ◽  
Herpès Virus ◽  
Marrow Cells

Abstract In recent studies, the sequence of Kaposi's sarcoma-associated herpes virus (KSHV) or human herpes virus-8 (HHV-8) was detected in dendritic cells (DC) of patients with multiple myeloma (MM). A concern was raised whether there is an causal association between the viral infection and development of these tumors. In the present study, we have examined DC generated from blood adherent cells from 8 Swedish MM patients at different clinical stages and 2 patients with monoclonal gammopathy of undetermined significance. In addition, 6 myeloma cell lines and bone marrow cells from 2 MM patients were also studied. By polymerase chain reaction (PCR), including nested PCR, no virus DNA was demonstrable in the patients' DC or in myeloma cell lines or fresh bone marrow cells. Moreover, no antibody against KSHV was found in the serum of these 10 patients. Thus, our results indicate that blood-derived DC of MM patients in Sweden usually are not infected with KSHV/HHV-8. This study also suggests that KSHV/HHV-8 is not regularly associated with MM and consequently does not play a primary role in the pathogenesis of these tumors.

scholarly journals A role for MALT1 activity in Kaposi’s sarcoma-associated herpes virus latency and growth of primary effusion lymphoma

Leukemia ◽  
2016 ◽  
Vol 31 (3) ◽  
pp. 614-624 ◽  
Author(s):  
L Bonsignore ◽  
K Passelli ◽  
C Pelzer ◽  
M Perroud ◽  
A Konrad ◽  
...  
Keyword(s):  
Kaposi's Sarcoma ◽  
Herpes Virus ◽  
Primary Effusion Lymphoma ◽  
Kaposi’S Sarcoma ◽  
Virus Latency ◽  
Herpès Virus

scholarly journals Primary effusion lymphoma in women: report of two cases of Kaposi's sarcoma herpes virus-associated effusion-based lymphoma in human immunodeficiency virus-negative women

Blood ◽  
1996 ◽  
Vol 88 (8) ◽  
pp. 3124-3128 ◽  
Author(s):  
JW Said ◽  
T Tasaka ◽  
S Takeuchi ◽  
H Asou ◽  
S de Vos ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
B Cell ◽  
Kaposi's Sarcoma ◽  
Herpes Virus ◽  
Primary Effusion Lymphoma ◽  
Kaposi’S Sarcoma ◽  
Chain Gene ◽  
Molecular Evidence ◽  
Immunodeficiency Virus ◽  
Herpès Virus

Recent molecular evidence suggests an association with a new herpes virus, Kaposi's sarcoma-associated herpes virus (KSHV/HHV-8), and primary effusion lymphomas (PEL). PELs have a characteristic morphology, phenotype, and clinical presentation with malignant effusions in the absence of a contiguous solid tumor mass. Most cases of PEL have occurred in human immunodeficiency virus (HIV)-positive male patients who are coinfected with Epstein-Barr virus (EBV). This report describes two cases of PEL in HIV- and EBV-negative women. In one patient, a pleural cavity PEL was preceded by classic Kaposi's Sarcoma (KS) of the lower extremities. In the second patient, PEL developed in an artificial cavity related to the capsule of a breast implant. Both cases had the characteristic morphologic appearance of high-grade anaplastic/B-cell immunoblastic lymphomas, with loss of B-cell differentiation antigens, clonal immunoglobulin heavy chain gene rearrangements, and expression of activation antigen CD30. Both cases were negative for EBV, herpes virus simplex, and cytomegalovirus (CMV). DNA extracted from both lymphomas and skin KS specimen showed KSHV sequences by molecular analysis. This report expands the spectrum of KSHV-associated disease to include PEL in HIV-negative women.

Involvement of Interleukin-10 (IL-10) and Viral IL-6 in the Spontaneous Growth of Kaposi’s Sarcoma Herpesvirus-Associated Infected Primary Effusion Lymphoma Cells

Blood ◽  
1999 ◽  
Vol 94 (8) ◽  
pp. 2871-2879 ◽  
Author(s):  
Karen D. Jones ◽  
Yoshiyasu Aoki ◽  
Yuan Chang ◽  
Patrick S. Moore ◽  
Robert Yarchoan ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Cell Lines ◽  
Neutralizing Antibodies ◽  
Kaposi's Sarcoma ◽  
Primary Effusion Lymphoma ◽  
Kaposi’S Sarcoma ◽  
Distinct Type ◽  
Interleukin 10 ◽  
Autocrine Growth

Primary effusion lymphoma (PEL) is a distinct type of lymphoma associated with Kaposi’s sarcoma-associated herpesvirus (KSHV) infection. To determine the factors responsible for the unrestrained proliferation of PEL, we have studied the growth factor requirements of the PEL-derived BCBL-1 and BC-1 cell lines. Both cell lines were found to be autocrine growth factor dependent and to release human interleukin-6 (IL-6), viral IL-6 (vIL-6), and human IL-10 in the culture supernatant. To establish whether these cytokines contribute to autocrine growth, neutralizing antibodies against human IL-6, vIL-6, human IL-10, and soluble IL-10 receptor were used. These experiments showed that human IL-10 and, to a lesser degree, vIL-6 serve as autocrine growth factors for BCBL-1 and BC-1 cells. Thus, human IL-10 and vIL-6 are growth factors released and used by PEL cells for autonomous proliferation and may be critical to the development and progression of PEL.

MOLECULAR DETECTION OF KAPOSI’S SARCOMA ASSOCIATED VIRUS (HUMAN HERPES VIRUS 8) IN SINO-NASAL CARCINOMA TISSUES

2020 ◽  
Vol 23 (04) ◽  
pp. 286-297
Author(s):  
Hind Hamed Shaker ◽  
Luma Amer Yasir ◽  
Ausama Abed-Alkadum Alajeely ◽  
Saad Hasan Mohammed Ali ◽  
Shakir H. Mohammed Al-Alwany
Keyword(s):  
Kaposi's Sarcoma ◽  
Herpes Virus ◽  
Molecular Detection ◽  
Kaposi’S Sarcoma ◽  
Human Herpes ◽  
Human Herpes Virus 8 ◽  
Human Herpes Virus ◽  
Herpès Virus ◽  
Nasal Carcinoma
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