scholarly journals Clinical features and phylogenetic analysis of Coxsackievirus A9 in Northern Taiwan in 2011

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Yi-Chuan Huang ◽  
Ying-Hsia Chu ◽  
Ting-Yu Yen ◽  
Wen-Chan Huang ◽  
Li-Min Huang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Clinical Features ◽  
Coxsackievirus A9 ◽  
Northern Taiwan

Dynamics of Apis cerana Sacbrood Virus (AcSBV) Prevalence in Apis cerana (Hymenoptera: Apidae) in Northern Taiwan and Demonstration of its Infection in Apis mellifera (Hymenoptera: Apidae)

2019 ◽  
Vol 112 (5) ◽  
pp. 2055-2066 ◽  
Author(s):  
Chong-Yu Ko ◽  
Zong-Lin Chiang ◽  
Ruo-Jyun Liao ◽  
Zih-Ting Chang ◽  
Ju-Chun Chang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Apis Mellifera ◽  
Honey Bee ◽  
Apis Cerana ◽  
Cross Infection ◽  
Prevalence Rates ◽  
Sacbrood Virus ◽  
Northern Taiwan ◽  
Long Term Survey

AbstractSince 2016, Apis cerana sacbrood virus (AcSBV) has been recorded in Taiwan. It is epizootic in Apis cerana (Hymenoptera: Apidae) and causing serious loss of A. cerana. Herein, we performed a long-term survey of AcSBV prevalence in the populations of A. cerana in Northern Taiwan from January 2017 to July 2018. The surveillance of AcSBV prevalence in A. mellifera (Hymenoptera: Apidae) populations was starting and further confirmed by sequencing since April 2017; thus, these data were also included in this survey. In our survey, the average prevalence rates of AcSBV were 72 and 53% in A. cerana and A. mellifera, respectively, in 2017, which decreased to 45 and 27% in 2018. For the spatial analysis of AcSBV in two honey bee populations, Hsinchu showed the highest prevalence, followed by New Taipei, Yilan, Taipei, and Keelung, suggesting that AcSBV might have come from the southern part of Taiwan. Interestingly, the AcSBV prevalence rates from A. cerana and A. mellifera cocultured apiaries gradually synchronized. The result of phylogenetic analysis and comparison of the annual AcSBV prevalence in A. cerana-only, A. mellifera-only, and A. cerana/A. mellifera cocultured sample sites indicate cross-infection between A. cerana and A. mellifera; however, AcSBV may lose the advantage of virulence in A. mellifera. The evidence suggested that the transmission of AcSBV might occur among these two honey bee species in the field. Therefore, A. mellifera may serve as a guard species to monitor AcSBV in A. cerana, but the cross-infection still needs to be surveyed.

Clinical features and phylogenetic analysis of severe hand-foot-and-mouth disease caused by Coxsackievirus A6

2020 ◽  
Vol 77 ◽  
pp. 104054 ◽  
Author(s):  
Xiaohan Yang ◽  
Yuanyuan Li ◽  
Changbin Zhang ◽  
Wenli Zhan ◽  
Jia Xie ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Clinical Features ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Coxsackievirus A6 ◽  
Foot And Mouth

scholarly journals Molecular Epidemiology and Clinical Features of Enteroviruses-Associated Hand, Foot, and Mouth Disease and Herpangina Outbreak in Zunyi, China, 2019

2021 ◽  
Vol 8 ◽  
Author(s):  
Yuanhang Ai ◽  
Weiwei Zhang ◽  
Jie Wu ◽  
Jingzhi Zhang ◽  
Meijing Shen ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Epidemiology ◽  
Clinical Features ◽  
Clinical Characteristics ◽  
Laboratory Data ◽  
Foot And Mouth Disease ◽  
Severe Infection ◽  
Mouth Disease ◽  
Vp1 Gene ◽  
Foot And Mouth

Background: Hand, foot and mouth disease (HFMD) and herpangina (HA), two of the most common childhood infectious diseases, are associated with enteroviruses (EVs) infection. The aim of this study was to identify the molecular epidemiology of enterovirus causing HFMD/HA in Zunyi, China, during 2019, and to describe the clinical features of the cases.Methods: We collected the information on demographic and clinical characteristics, laboratory data of laboratory-confirmed EVs associated HFMD/HA cases in Zunyi Medical University Third Affiliated Hospital between March 1 and July 31, 2019. EV types were determined by either one-step real time RT-PCR or partial VP1 gene sequencing and sequence alignment. Phylogenetic analysis of CVA6, CVA2, and CVA5 were established based on the partial VP1 gene sequences by neighbor-joining method. Differences in clinical characteristics and laboratory results of the cases were compared among patients infected with the most prevalent EV types.Results: From 1 March to 31 July 2019, 1,377 EVs associated HFMD/HA inpatients were confirmed. Of them, 4 (0.3%, 4/1,377) were EV-A71-associated cases, 84 (6.1%, 84/1,377) were CVA16-associated cases, and 1,289 (93.6%, 1,289/1,377) were non-EV-A71/CVA16-associated cases. Of the randomly selected 372 non-EV-A71/CVA16 cases, EV types have been successfully determined in 273 cases including 166 HFMD and 107 HA cases. For HFMD cases, the three most common types were CVA6 (80.7%, 134/166), CVA2 (5.4%, 9/166) and CVA5 (3.0%, 5/166); similarly, for HA cases, the three most prevalent serotypes were CVA6 (36.5%, 39/107), CVA2 (21.5%, 23/107) and CVA5 (18.7%, 20/107). Phylogenetic analysis showed that subclade D of CVA5, and subclade E of CVA6 and CVA2 were predominant in Zunyi during the outbreak in 2019. Compared with the cases caused by CVA16, the incidence of high fever and severe infection associated with CVA2, CVA5, and CVA6 was higher.Conclusions: The recent HFMD/HA outbreak in Zunyi is due to a larger incidence of CVA6, CVA2, and CVA5. Novel diagnostic reagents and vaccines against these types would be important to monitor and control EV infections.

scholarly journals Human bocavirus (HBoV) in Kuwait: molecular epidemiology and clinical outcome of the virus among patients with respiratory diseases

2020 ◽  
Vol 69 (7) ◽  
pp. 1005-1012
Author(s):  
Nada M. Madi ◽  
Anfal Al-Adwani
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Epidemiology ◽  
Clinical Features ◽  
Respiratory Diseases ◽  
Genetic Characterization ◽  
Respiratory Viruses ◽  
Respiratory Pathogen ◽  
Human Bocavirus ◽  
Sequence Variations

Introduction. Globally, human bocavirus (HBoV) has been detected in respiratory samples from patients suffering from upper and lower respiratory diseases. In Kuwait, little is known about the epidemiological and clinical characterization of the virus and genetic characterization of the virus as a respiratory pathogen is unknown. Aim. This study aims to explore the molecular epidemiology and clinical features of HBoV isolates in patients with respiratory diseases. Methodology. Retrospectively, between 2018 and 2020, 5941 respiratory samples from patients with respiratory diseases were screened for respiratory viruses using multiplex real-time PCR. Samples that were positive for HBoV were then subjected to NP1 and VP1/PV2 phylogenetic analysis. Results. HBoV was detected in 1.9 % of the patients, with a peak incidence of infection among children <1 year old. Co-infection with other respiratory viruses was observed in 56.8 % of HBoV-positive patients. Fever, cough and respiratory distress were the most common clinical features of HBoV infection. Phylogenetic analysis of the Kuwaiti HBoV isolates revealed that all the isolates were of the HBoV-1 genotype, with slight sequence variations among the isolates. Conclusion. This study illustrated the predominance of the HBoV-1 genotype in patients with respiratory diseases in Kuwait with minimal genetic variability. It also highlighted the clinical features of HBoV-1 infection, verifying its role in respiratory diseases.

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