Dushivisha is a unique concept of Ayurveda. Bruhatrayi explains spectrum of Dushivisha concept in many ways as a important concept related in Vishatatra statement. Dushivisha is any low potency toxic substance which vitiates the Dhatus when favorable condition occurs. It produces different symptoms in different people according to their age, habits and place of residence. In the Ayurveda context the poison has been divided into sub types and there is no separate classification of Dushivisha, But Ayurveda defines it as Sthavara, Jangama or Kritimavisha after its treatment, when it becomes less potent its effects are doesn’t nullifies radically because of which it resides in the body, that particular less potent part of above said poison is called Dushivisha. Dushivisha produces sense of intoxication after meals, indigestion, anorexia, eruption of circular patches on the skin, urticaria, mental confusion, Dhatukashya, oedema on the face and extremities, ascites, vomiting, diarrhoea, discolouration, fainting, intermittent high grade fever and unquenched able thirst. Some poisons produce insanity, abdominal distension, Shukra Kshaya, muffled voice while other causes Kustha and respective disorders of various type. Acharya Shusruta mention Ajay Gritha in the treatment of Dushivisha. So this article aim to collect the data to evaluate the effect of Ajay Ghrita in Dushivishajanya Lakshana.