scholarly journals Multiple antibiotic resistances among Shiga toxin producing Escherichia coli O157 in feces of dairy cattle farms in Eastern Cape of South Africa

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Benson C. Iweriebor ◽  
Chinwe J. Iwu ◽  
Larry C. Obi ◽  
Uchechukwu U. Nwodo ◽  
Anthony I. Okoh
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Dairy Cattle ◽  
Shiga Toxin ◽  
Escherichia Coli O157 ◽  
Eastern Cape ◽  
Cattle Farms

Association of Herd Management Factors with Colonization of Dairy Cattle by Shiga Toxin-Positive Escherichia coli O157

1998 ◽  
Vol 61 (7) ◽  
pp. 802-807 ◽  
Author(s):  
DONALD E. HERRIOTT ◽  
DALE D. HANCOCK ◽  
ERIC D. EBEL ◽  
LINDA V. CARPENTER ◽  
DANIEL H. RICE ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dairy Cattle ◽  
Pacific Northwest ◽  
Shiga Toxin ◽  
Management Practices ◽  
Animal Husbandry ◽  
Dairy Herds ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Management Factors

Management factors in 36 Pacific Northwest dairy herds were evaluated for their association with the prevalence of Shiga toxin-positive Escherichia coli O157 (E. coli O157) in dairy cattle. The within-herd prevalence of E. coli O157 was estimated by bacteriological culture of fecal pat samples, collected monthly for 6 months (approximately 60 per visit), from heifer cattle. During the first visit to each farm, a management questionnaire was administered that covered a broad range of animal husbandry practices. On each subsequent visit, a brief questionnaire was administered to detect changes in management practices. A significantly higher prevalence of E. coli O157 was noted in herds that fed com silage to heifers compared to herds that did not feed com silage. More tentative associations of E. coli O157 prevalence were observed for weaning method, protein level of calf starter, feeding of ionophores in heifer rations, feeding of grain screens to heifers, and feeding of animal by-products to cows.

Escherichia coli O157 Prevalence in Different Cattle Farm Types and Identification of Potential Risk Factors

2009 ◽  
Vol 72 (9) ◽  
pp. 1848-1853 ◽  
Author(s):  
K. COBBAUT ◽  
D. BERKVENS ◽  
K. HOUF ◽  
R. DE DEKEN ◽  
L. DE ZUTTER
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Dairy Cattle ◽  
Farm Size ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Multiplex Pcr Assay ◽  
Farm Type ◽  
Veal Calf ◽  
Cattle Farms

Although the prevalence of Escherichia coli O157 on cattle farms has been examined extensively, the relationship between this pathogen and farm type has been established only rarely. A large-scale study was designed to determine the prevalence of E. coli O157 in the Flemish region of Belgium on farms of dairy cattle, beef cattle, mixed dairy and beef cattle, and veal calves. The effect of various factors on the occurrence at the pen level also was evaluated. In 2007, 180 farms were randomly selected based on region, farm size, and number of animals purchased and were examined using the overshoe sampling method. When possible, overshoes used in areas containing animals in three different age categories (<8 months, 8 to 30 months, and >30 months) were sampled on each farm. In total, 820 different pens were sampled and analyzed for the presence of E. coli O157 by enrichment, immunomagnetic separation, and plating on selective agar. Presumptive E. coli O157 colonies were identified using a multiplex PCR assay for the presence of the rfbO157 and fliCH7 genes. The statistical analysis was carried out with Stata SE/10.0 using a generalized linear regression model with a logit link function and a binomial error distribution. The overall farm prevalence of E. coli O157 was 37.8% (68 of 180 farms). The highest prevalence was found on dairy cattle farms (61.2%, 30 of 49 farms). The prevalences on beef, mixed dairy and beef, and veal calf farms were 22.7% (17 of 75 farms), 44.4% (20 of 45 farms), and 9.1% (1 of 11 farms), respectively. A significant positive correlation between age category and E. coli O157 prevalence was found only on mixed dairy and beef farms and dairy farms. No influence of farm size or introduction of new animals was demonstrated.

Prevalence and Antimicrobial Susceptibility of Escherichia coli O157:H7 in Vegetables Sold in the Amathole District, Eastern Cape Province of South Africa

2008 ◽  
Vol 71 (4) ◽  
pp. 816-819 ◽  
Author(s):  
B. O. ABONG'O ◽  
M. N. B. MOMBA ◽  
J. N. MWAMBAKANA
Keyword(s):  
Escherichia Coli ◽  
South Africa ◽  
Antimicrobial Susceptibility ◽  
Molecular Techniques ◽  
Escherichia Coli O157 ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
E Coli ◽  
Fresh Vegetables

Fresh vegetables have been implicated in outbreaks of Escherichia coli O157:H7 in most parts of the world. Microbiological quality of vegetables used as recipes for salads is very crucial. Residents of the Amathole District in the Eastern Cape Province of South Africa consume salads frequently, although the microbial quality of recipe vegetables is questionable. The present study investigated the prevalence and antimicrobial susceptibility of E. coli O157:H7 isolated from selected vegetables sold within the Amathole District. One hundred eighty samples of the vegetables were analyzed. Strains of E. coli O157:H7 were isolated by enrichment culture and by immunomagnetic separation and identified by conventional and molecular techniques. In three settlements in this district, the mean counts of presumptive E. coli O157 for the vegetables ranged between 9 × 103 and 1.6 × 106 CFU/g for Fort Beaufort, 1.6 × 103 and 1.6 × 105 CFU/g for Mdantsane, and 1.3 × 103 and 4.1 × 104 CFU/g for Alice. Four (10.3%) of 39 vegetable samples were confirmed to carry E. coli O157:H7. Four representative E. coli O157:H7 isolates from these vegetables were susceptible to at least one of the eight antimicrobial agents tested against them. Even though the prevalence of E. coli O157:H7 was low and those isolated were susceptible to most of the antimicrobials, there remains a need for E. coli O157:H7 surveillance in vegetables used in salad recipes in urban and rural areas of South Africa.

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