scholarly journals Expression of mRNA vascular endothelial growth factor in hypospadias patients

BMC Urology ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Prahara Yuri ◽  
Gunadi ◽  
Rahmadani P. Lestari ◽  
Firly P. Fardilla ◽  
Ishandono Dachlan
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Fold Change ◽  
Vascular Endothelial ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Distal Hypospadias ◽  
Endothelial Growth Factor ◽  
Larger Sample

Abstract Background Hypospadias is a relatively common genital anomaly in humans, usually followed by inelastic dartos that causes penile chordee. Vascular endothelial growth factor (VEGF) is strongly linked to the viscoelasticity of tissues and their elastic phase. This study aimed to evaluate VEGF expressions in (1) fascia dartos between hypospadias and controls and (2) chordee severity. Methods This prospective cohort study involved 65 specimens from patients with hypospadias and ten specimens from controls. The samples were analyzed by quantitative real-time polymerase chain reaction (qPCR) for VEGF expression. Results The expressions of VEGF were not different between proximal and distal hypospadias patients and controls (fold change: distal − 0.25; fold change: proximal − 0.2; p = 0.664). The scaled expressions related to chordee severity were mild − 0.1; moderate 0.1; severe − 0.25 (p = 0.660). Conclusions VEGF expressions might not affect the severity of hypospadias and chordee, implying the pathogenesis is complex involving many growth factors. Further study with a larger sample size is necessary to clarify and confirm our findings.

Effects of shRNA Targeting Maspin on the Invasion of Extravillous Trophoblast Cell

2017 ◽  
Vol 34 (10) ◽  
pp. 0966-0973 ◽  
Author(s):  
Xinwei Shi ◽  
Qing Liu ◽  
Hao Liu ◽  
Dongrui Deng ◽  
Fuyuan Qiao ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Vascular Endothelial ◽  
Chain Reaction ◽  
Maspin Expression ◽  
Polymerase Chain ◽  
Endothelial Growth Factor

Objective Hypomethylation of the maspin gene results in increased expression of maspin in preeclamptic placentas. However, maspin gene function and the molecular aspects in placentation remain largely unclear. The study was designed to investigate the effects of maspin on the invasion of extravillous trophoblast cell line (TEV-1) and the molecular mechanism. Study Design We cloned short hairpin RNA (shRNA) targeting maspin gene into plasmid pGenesil-1.1 eukaryotic expression vector and then transfected it using adenovirus. The methylation rates in the maspin gene were detected by bisulfite sequencing polymerase chain reaction; the invasive ability of trophoblast cells was examined by Transwell chamber assay; the mRNA and protein expression of maspin and some invasive related gene was detected by reverse transcription-polymerase chain reaction and Western blot analysis. Results After the maspin expression was successfully knocked down, the methylation rates in the maspin gene were significantly increased, and the number of cells invading through Matrigel (Corning Life Sciences and BD Biosciences) was obviously increased. The mRNA levels of vascular endothelial growth factor-A (VEGF-A), vascular endothelial growth factor-C (VEGF-C), and matrix metalloproteinase-2 (MMP2) were increased significantly. Conclusion Using shRNA technology, this study further verified that maspin gene methylation could decrease maspin expression and inhibit the invasion of TEV-1 cells through VEGF-A, VEGF-C, and MMP2.

Expression of vascular endothelial growth factor A isoforms is dysregulated in women with endometriosis

2018 ◽  
Vol 30 (4) ◽  
pp. 651 ◽  
Author(s):  
Kevin Danastas ◽  
Emily J. Miller ◽  
Alison J. Hey-Cunningham ◽  
Christopher R. Murphy ◽  
Laura A. Lindsay
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Vascular Endothelial ◽  
Chain Reaction ◽  
Natural Production ◽  
Polymerase Chain ◽  
Endothelial Growth Factor ◽  
Natural Expression

Angiogenesis is a critical step in the development of ectopic lesions during endometriosis. Although total vascular endothelial growth factor (VEGF) A is elevated in the peritoneal fluid of women with endometriosis, there are contradictory reports on how levels of total endometrial VEGFA are altered in this disease. Furthermore, limited research is available on different VEGFA isoforms in women with endometriosis. Thus, the aim of the present study was to analyse levels of various VEGFA isoforms in women with and without endometriosis at different stages of the menstrual cycle. Quantitative polymerase chain reaction analysis showed that total VEGFA was highest during menstruation in endometriosis compared with controls (P = 0.0373). VEGF121 and VEGF189 were similarly highest during menstruation in endometriosis compared with controls (P = 0.0165 and 0.0154 respectively). The present study is also the first to identify the natural expression of VEGF111 in human tissue, which is also highest during menstruation in endometriosis (P = 0.0464). This discovery of the natural production of VEGF111 in human endometrium, as well as the upregulation of VEGFA isoforms during menstruation in endometriosis, may shed further light on the development and progression of the disease, and improve our understanding of the regulation of endometrial angiogenesis.

Quantification of hypoxia-inducible factor-1α, vascular endothelial growth factor, and angiopoietin-2 expression in hepatocellular carcinoma using a real-time quantitative reverse transcription polymerase chain reaction.

2011 ◽  
Vol 29 (4_suppl) ◽  
pp. 223-223
Author(s):  
B. Sharma ◽  
R. Srinivasan ◽  
Y. K. Chawla ◽  
A. Chakraborty ◽  
S. Kapil ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Vascular Endothelial Growth Factor ◽  
Polymerase Chain Reaction ◽  
Chronic Hepatitis ◽  
Hypoxia Inducible Factor ◽  
Vascular Endothelial ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
The Difference ◽  
Endothelial Growth Factor

223 Background: Hepatocellular carcinoma (HCC) is characteristically a hypervascular tumor and its progression is closely related to angiogenesis. Hypoxia inducible factor-1α (HIF-1 α), vascular endothelial growth factor (VEGF), and angiopioetin-2 (Ang-2) are key angiogenic factors in HCC. In this study, expression of HIF-1α, VEGF, and Ang-2 were analyzed and correlated with clinicopathologic features of HCC. Methods: Expression of HIF-1α, VEGF, and Ang-2 were analyzed by real-time quantitative reverse transcription polymerase chain reaction in 67 HCC patients (23HBV, 22HCV, 22NBNC) with mean age 59.09 ± 11.13 years (60 M: 7 F), 9 cirrhosis patients (2HBV, 2HCV, 5ALD) with mean age 48.33 ± 11.31 years (8 M:1 F), and 14 chronic hepatitis patients (7HBV, 7HCV) with mean age 48.33 ± 7.17 years (10 M: 4 F), respectively. HCC was diagnosed on the basis of EASL (European Association for the Study of the Liver) criteria. Results: Expression of HIF-1α and VEGF were found to be significantly higher in patients with HCC compared with cirrhosis and chronic hepatitis. Although Ang-2 expression was higher in HCC compared to cirrhosis and chronic hepatitis but the difference was not statistically significant. The difference in the expression of HIF-1α, VEGF, and Ang-2 in cirrhosis and chronic hepatitis were not significant. Tumor size was positively correlated with HIF-1 α(p = 0.268, p = 0.030)and VEGF (p = 0.546, p = 0.0001). A positive correlation was also seen between the HIF-1α and VEGF expression (p = 0.347, p = 0.0001). However no correlation of HIF-1α, VEGF and Ang-2 was seen with portal vein thrombosis, cirrhosis, etiology, AST, ALT, and platelets. Conclusions: HIF-1α and VEGF were highly expressed in HCC patients and may be responsible for the HCC growth. Further studies with a larger number of patients need to be done. No significant financial relationships to disclose.

scholarly journals Exploring of insertion/deletion polymorphism in the vascular endothelial growth factor gene associated with type two diabetic patients in Al-Diwaniyah City

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Abbas G Hamead
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Age Factors ◽  
The Other ◽  
Diabetic Patients ◽  
Vascular Endothelial ◽  
Growth Factor Gene ◽  
Polymerase Chain ◽  
Endothelial Growth Factor ◽  
Genotypic Characteristic

Disease conditions that are related to the effects of the vascular endothelial growth factor (VEGF) are common such as nephropathy and promoting of cancer growth and metastasis. The current study was launched to explore the presence of VEGF gene-based polymorphism of the insertion/deletion (I/D) in blood samples of 25 diabetic and 25 clinically healthy people in Al-Diwaniyah City, Iraq. Using polymerase chain reaction (PCR), the results have shown the presence of these genotypes in these samples with higher significant (p˂0.05) occurrence. The homozygous DD was significantly (p˂0.05) affected by the sex factor. This genotypic characteristic was shown significantly (p˂0.05) in females more than that in males. Moreover, this feature was significantly higher (p˂0.05) when compared to the other genotypes II and I/D for each of the females or males separately. For the DD genotype,ages between 40 to 49 years of old have shown significantly (p˂0.05) higher presence of this genotype than that in other ages. It has been also shown by these results that this genotype is present significantly (p˂0.05) higher than that in the other genotypes when compared together in each age category. These results suggest current relationship between the VEGF I/D polymorphism occurrence and diabetes with deeper correlation with sex and age factors.

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