scholarly journals The evaluation of cytotoxicity and cytokine IL-6 production of root canal sealers with and without the incorporation of simvastatin: an invitro study

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Apoorva Sharma ◽  
Kavitha Sanjeev ◽  
Vinola M. J. Selvanathan ◽  
Mahalaxmi Sekar ◽  
Nikhil Harikrishnan
Keyword(s):  
Zinc Oxide ◽  
Root Canal ◽  
Inflammatory Marker ◽  
Pairwise Comparison ◽  
Mouse Fibroblast ◽  
L929 Cells ◽  
Inflammatory Reactions ◽  
Zinc Oxide Eugenol ◽  
Time Periods ◽  
Root Canal Sealers

Abstract Background Freshly mixed root canal sealers when proximate the periapical tissues, trigger varying degrees of cytotoxicity/inflammatory reactions. Simvastatin, a class of the drug statin, is a widely used cholesterol-lowering agent with additional anti-inflammatory activities. This study assessed the effects of simvastatin on cytotoxicity and the release of IL-6 (Interleukin-6) production when incorporated in zinc oxide eugenol and methacrylate resin-based sealers. Methods Experimental groups consisted of conventional zinc oxide eugenol and methacrylate based-EndoREZ sealers (ZE & ER respectively) and 0.5 mg/mL simvastatin incorporated sealers (ZES & ERS). L929 mouse fibroblast cells were exposed to freshly mixed experimental sealers and evaluated for cytotoxicity (MTT assay) and inflammation levels (inflammatory marker IL-6 for ELISA) at various time intervals (0h, 24h and 7th day). The values were compared to the cell control (CC; L929 cells alone) and solvent control (SC; L929 cells + DMSO) groups. All the experiments were conducted in triplicates and subjected to statistical analysis using IBM SPSS Statistics software. Non parametric tests were conducted using Kruskal-Wallis and Friedman tests for inter-group and intra-group comparisons respectively. Pairwise comparison was conducted by post hoc Dunn test followed by Bonferroni correction. P values < 0.05 were considered statistically significant. Results All the experimental groups (ZE, ER, ZES, ERS) exhibited varying degree of cytotoxicity and IL-6 expression compared to the control groups CC and SC. The cell viability for ZE and ER decreased on day 7 as compared to 24 h. ZES and ERS had higher viable cells (75.93% & 79.90%) compared to ZE and ER (54.39% & 57.84%) at all time periods. Increased expression of IL-6 was observed in ZE & ER (25.49 pg/mL & 23.14 pg/mL) when compared to simvastatin incorporated ZE & ER (ZES-12.70 pg/mL & ERS-14.68 pg/mL) at all time periods. Highest level of cytotoxicity and inflammation was observed in ZE compared to all the other groups on day 7. Conclusions Addition of 0.5 mg/mL of simvastatin to the sealers (ZES and ERS) decreased the cytotoxicity in the freshly mixed state and reduces their inflammatory effect.

scholarly journals A Cross-Sectional Study on the Preference of Root Canal Sealers Among Pediatric Dentists For Obturating Permanent Teeth

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 226-232
Author(s):  
Lakshmi Lakshmanan ◽  
Subramanian EMG ◽  
Ganesh Jeevanandan ◽  
Mebin George Mathew
Keyword(s):  
Zinc Oxide ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Cross Sectional Study ◽  
Permanent Teeth ◽  
Cross Sectional ◽  
Zinc Oxide Eugenol ◽  
Significant Difference ◽  
Pediatric Dentists ◽  
Root Canal Sealers

Utilisation of root canal sealers to perform obturation procedures is an endorsed method in endodontics and has an indispensable role in the success of treatment. The objective of this study was to explore the pediatric dentists’ preference for root canal sealers for obturating permanent teeth. 360 permanent teeth with gutta-percha obturation done by pediatric dentists were analyzed for the study. The variety of root canal sealers that are utilised for obturating the permanent teeth by pediatric dentists were assessed. The data were obtained from a patient management software. Chi-square test was performed. Out of the 360 permanent teeth, 186 teeth were obturated using resin-based sealers, 101 teeth using zinc oxide eugenol-based sealers and 73 teeth were obturated using calcium hydroxide-based sealers, with statistically significant difference between the three materials (P<0.05). Based on the findings of the present study, resin sealers followed by zinc oxide eugenol sealers and calcium hydroxide sealers were more preferred by pediatric dentists for root canal therapy in permanent teeth. 

scholarly journals Root canal sealers in dental practice

2020 ◽  
Vol 11 (SPL3) ◽  
pp. 68-71
Author(s):  
Vinaya Swetha T ◽  
Dhanraj Ganapathy ◽  
Kiran Kumar
Keyword(s):  
Zinc Oxide ◽  
Root Canal ◽  
Endodontic Treatment ◽  
Questionnaire Study ◽  
Dental Practice ◽  
Online Forum ◽  
Private Practitioners ◽  
Zinc Oxide Eugenol ◽  
Knowledge And Attitude ◽  
Root Canal Sealers

Root canal sealers are important materials commonly used in endodontic treatment. Endodontic treatment for cleaning the root canal and removing the pulp and filling with manmade materials. The aim of the study was to assess the knowledge and attitude of the practitioners regarding root canal sealers in endodontic treatment. It is an online-based questionnaire study in which ten questions were circulated through an online forum through google forms and results were analysed using SPSS. In this study, it was observed that private practitioners most commonly use zinc oxide eugenol based sealers (50%), but it shows that methacrylate resin-based sealers (50%) are most effective in sealing. It shows that private practitioners are more aware of root canal sealers and its uses, and about its properties.

scholarly journals Antimicrobial activity of root canal sealers

2006 ◽  
Vol 53 (2) ◽  
pp. 104-112 ◽  
Author(s):  
Mirjana Vujaskovic ◽  
Branka Radosavljevic
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Epoxy Resin ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Glass Ionomer ◽  
Zinc Oxide Eugenol ◽  
Root Canal Sealers

All root canal sealers should have antimicrobial activity so that they could act against any remaining bacteria after root canal obturation. The aim of this study was to evaluate the antimicrobial activity of five root canal sealers: AH26, a resin based paste; Apexit, calcium hydroxide based paste; Endomethasone and Tubliseal, zinc oxide eugenol based materials and Ketac Endo Aplicap, glass ionomer based sealer. Matherials And Methods: Antimicrobial activity was tested against S. mutans 70C and L.casei ATCC 27773 using ADT (agar diffusion inhibitory test) on TYC SB, blood and MRS agars. Freshly prepared paste or cement was placed in grooves of 4 mm in diameter in agar plates. Prepared agar plates were incubated at 37?C in GAS PAC system for 24hours. The antimicrobial effect was determined according to the diameter of growth inhibition zone around tested materials. Three measurements were done for each material and values were recorded in millimeters. Statistical analysis was done using Mann Whitney test. The results confirmed that epoxy resin and zinc oxide eugenol based sealers had the greatest antimicrobial effect. Calcium hydroxide and glass ionomer based sealers showed significantly lower antimicrobial activity compared to AH26, Endomethasone and Tubliseal. Conclusion: The greatest antimicrobial activity was found for epoxy resin based sealer (AH26) for both tested microorganisms.

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