scholarly journals Assessment of salivary alpha-amylase and cortisol as a pain related stress biomarker in dogs pre-and post-operation

2022 ◽  
Vol 18 (1) ◽  
Author(s):  
Eun-Ha Kang ◽  
Seol-Hee Park ◽  
Ye-In Oh ◽  
Kyoung-Won Seo
Keyword(s):  
Pain Score ◽  
Serum Cortisol ◽  
Alpha Amylase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Salivary Alpha Amylase ◽  
Pain Scores ◽  
High Pain Score ◽  
Post Operation ◽  
Cortisol Levels ◽  
Related Stress

Abstract Background The use of salivary biomarkers has garnered attention because the composition of saliva reflects the body’s physiological state. Saliva contains a wide range of components, including peptides, nucleic acids, electrolytes, enzymes, and hormones. It has been reported that salivary alpha-amylase and cortisol are biomarkers of stress related biomarker in diseased dogs; however, evaluation of salivary alpha-amylase and cortisol pre- and post- operation has not been studied yet. The aim of this study was to evaluate salivary alpha-amylase and cortisol levels in dogs before and after they underwent surgery and investigate the association between the salivary alpha-amylase and cortisol activity and pain intensity. For this purpose, a total of 35 dogs with disease-related pain undergoing orthopedic and soft tissue surgeries were recruited. Alpha-amylase and cortisol levels in the dogs’ saliva and serum were measured for each using a commercially available canine-specific enzyme-linked immunosorbent assay kit, and physical examinations (measurement of heart rate and blood pressure) were performed. In addition, the dogs’ pre- and post-operative pain scores determined using the short form of the Glasgow Composite Measure Pain Scale (CMPS-SF) were evaluated. Results After surgery, there was a significant decrease in the dogs’ pain scores (0.4-fold for the CMPS-SF, p < 0.001) and serum cortisol levels (0.73-fold, p < 0.01). Based on their pre-operative CMPS-SF scores, the dogs were included in either a high-pain-score group or a low-pain-score group. After the dogs in the high-pain-score group underwent surgical intervention, there was a significant decrease in their CMPS-SF scores and levels of salivary alpha-amylase, serum alpha-amylase, and serum cortisol. Additionally, there was a positive correlation between salivary alpha-amylase levels and CMPS-SF scores in both the high- and low-pain-score groups. Conclusions The measurement of salivary alpha amylase can be considered an important non-invasive tool for the evaluation of pain-related stress in dogs.

scholarly journals Study of Salivary Stress Factor Activity in Patients with Diabetes Mellitus During the Period of Adaptation to Complete Removable Laminar Dentures

2020 ◽  
Vol 27 (3) ◽  
pp. E202038
Author(s):  
Lyubov Leybyuk ◽  
Mykola Rozhko ◽  
Olena Rozhko
Keyword(s):  
Diabetes Mellitus ◽  
Salivary Cortisol ◽  
Acrylic Resin ◽  
Stress Factor ◽  
Alpha Amylase ◽  
Salivary Alpha Amylase ◽  
Group I ◽  
Patients With Diabetes ◽  
Cortisol Levels ◽  
Edentulous Patients

Diabetes mellitus is referred to as non-communicable epidemic of the 21st century and the fight against diabetes is one of medical and social challenges. One of its complications is damage to periodontal tissues that is accompanied by tooth loss. Any removable denture is a foreign body being a stress factor for the oral cavity. The objective of the research was to study dynamic changes in the activity of stress factor indicators, namely salivary cortisol, glucose and alpha-amylase levels in diabetic patients after prosthodontic treatment with complete removable laminar dentures. Materials and Methods. For this study, patients at the age of 45 to 74 years were divided into two groups: Group I included 52 edentulous patients with type 2 diabetes mellitus who were treated with acrylic resin complete removable laminar dentures; Group II comprised 52 edentulous patients without endocrine pathology who were treated with acrylic resin complete removable laminar dentures as well. Saliva served as the study material. The enzyme-linked immunosorbent assay was used to determine salivary cortisol levels. Glucose concentration in saliva was determined by a unified enzymatic method. Alpha-amylase activity was determined by the enzymatic colorimetric method. Results. According to the research results, before prosthodontic treatment, salivary cortisol levels of patients with diabetes mellitus were 2.4 times higher as compared to patients without somatic pathology. In phase I of adaptation to complete removable laminar dentures, in patients with diabetes mellitus, salivary cortisol concentration increased drastically by 1.4 times as compared to the indicator before prosthodontic treatment (5.61±0.83 ng/ml vs. 4.02±0.65 ng/ml, p<0.01), while in the group of patients without endocrine pathology, cortisol levels increased by 1.2 times; salivary glucose level of patients with diabetes mellitus increased by 1.3 times, while in patients without endocrine pathology, it increased by 1.1 times; in patients of Group I, salivary alpha-amylase levels increased by 1.2 times, while in patients of Group II, salivary alpha-amylase concentration increased by 1.05 times. Conclusions. In patients with diabetes mellitus, changes in saliva composition indicate a significant reduction in the adaptation mechanisms, that complicates and significantly prolongs the period of adaptation to complete removable laminar dentures.

scholarly journals Salivary Alpha Amylase Enzyme and Salivary Cortisol Level in Depression after Treatment with Fluoxetine

2021 ◽  
Vol 9 (T3) ◽  
pp. 144-149
Author(s):  
Andi Jayalangkara Tanra ◽  
Hawaidah Madeali ◽  
Mayamariska Sanusi ◽  
Saidah Syamsuddin ◽  
Sonny Teddy Lisal
Keyword(s):  
Salivary Cortisol ◽  
Depressive Disorders ◽  
Rating Scale ◽  
Antidepressant Drug ◽  
Alpha Amylase ◽  
Salivary Alpha Amylase ◽  
Potential Biomarker ◽  
Salivary Cortisol Level ◽  
Healthy Control ◽  
Cortisol Levels

BACKGROUND: Hypothalamic-pituitary-adrenal axis and its end product cortisol have been extensively investigated in patients with depressive disorders for many years. Recently, salivary alpha-amylase (sAA) had emerged as a new biomarker with non-invasive and more convenience protocol for measuring sympathetic activity which were also associated with depression. Selective Serotonin Reuptake Inhibitor is antidepressant drug extensively used to treat depression. AIM: The aim of this study was to determine whether decrease of sAA and salivary cortisol levels could be observed in subjects with depression who were treated by fluoxetine. METHODS: The total subjects were 25 depressed subjects and ten healthy controls. sAA was examined before therapy, and after 2, 4, and 6 weeks of fluoxetine administration using a portable cocorometer. Salivary cortisol was examined before therapy, after 4 and 6 weeks of fluoxetine administration with Elisa method. The therapeutic effect was assessed with Hamilton Depression Rating Scale (HDRS). RESULTS: sAA and cortisol levels were significantly decreased after fluoxetine administration (p < 0.001), followed by at least 50% reduction of HDRS scores after 6 weeks of fluoxetine administration. Levels of sAA and cortisol were higher in the depression group than in the healthy control. CONCLUSIONS: Measurement of sAA levels can be used as a potential biomarker of therapeutic response in depressed patients in addition to salivary cortisol.

scholarly journals Regulation of Cortisol in Patients Undergoing Total Joint Arthoplasty

2021 ◽  
Vol 27 ◽  
pp. 107602962198761
Author(s):  
Rajan Khanna ◽  
Hannah Slovacek ◽  
Jeffrey Liles ◽  
Sandra Haddad ◽  
Pavel Poredos ◽  
...  
Keyword(s):  
Emotional Stress ◽  
Total Joint Arthroplasty ◽  
Plasma Cortisol ◽  
Serum Cortisol ◽  
Joint Arthroplasty ◽  
Plasma Cortisol Level ◽  
Definitive Treatment ◽  
Elisa Kit ◽  
Post Operation ◽  
Cortisol Levels

Osteoarthritis is a condition in which joint cartilage and bone degenerate progressively over time. Total joint arthroplasty is a definitive treatment. Cortisol is a hormone that is associated with pain and inflammation. This study aims to investigate the cortisol levels in patients undergoing total joint arthroplasty. Plasma samples were collected from 71 total joint arthroplasty (TJA) patients at baseline (pre-surgery), 24 hours post-operation, and 5 days post-operation. Cortisol levels were measured in each sample using a commercially available ELISA kit. All results were compiled as group means ± SD. The plasma cortisol level at baseline were 218.5 ± 12 ng/mL. The 24-hour post-surgical samples showed a marked increase in cortisol levels 240.7 ± 15 ng/mL. The blood samples drawn at the 5th day after surgery showed a downward trend (74 ± 12 ng/mL). At 5 days post-operation, cortisol levels were significantly lower than at baseline or 24 hours post-operation. These results point to the fact that prior to surgery, the patient’s emotional stress contributes to increased serum cortisol levels. The higher level of cortisol persists at 24 hours post-operation due to inflammation from the procedure. This data also suggests that at 5 days post-operation, the inflammatory response from the surgery and emotional stress subside, resulting in a near normalization of the cortisol levels. Cortisol is a hormone that plays a major role in the body’s response to surgery. The relevance between cortisol and different points in the surgical timeline has the potential to prognosticate and improve recovery measures.

scholarly journals Evaluation of hormonal profile and some stress biomarkers in infertile couples in Abuja, Nigeria

2020 ◽  
Vol 8 (4) ◽  
pp. 1514
Author(s):  
Simon P. Abriba ◽  
Humphrey B. Osadolor
Keyword(s):  
Alpha Amylase ◽  
Serum Prolactin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Sperm Cells ◽  
Study Group ◽  
Salivary Alpha Amylase ◽  
Hormonal Profile ◽  
Stress Biomarkers ◽  
Infertile Couples

Background: Infertility is a growing gynaecological problem in couples of childbearing age having difficulties bearing children. Couples with infertility are known to present with high levels of stress and psychopathology A cross sectional case control study aimed at evaluating the hormonal profile, some stress biomarkers, sperm analysis in infertile couples was carried out to ascertain their contributions to infertility in couples of child bearing age.Methods: Serum Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH), Prolactin, Progesterone, Estrogen (E2), Testosterone, Salivary Cortisol and Salivary Alpha Amylase were evaluated using both competitive and non-competitive Enzyme Linked Immunosorbent Assay (ELISA) techniques; while sperm cells analysis were evaluated using conventional methods, in 164 infertile couples (study) and 100 fertile couples (control) attending fertility clinic in General Hospitals in Abuja, Nigeria.Results: The mean serum Prolactin and Salivary (S) Cortisol in the female were significantly higher (p<0.05) in the study group relative to the control group, while Salivary Alpha Amylase and LH show no significant difference (p>0.05) between the two groups. FSH, Progesterone and E2 in the female were significantly lower in the study group (p<0.05) compared with control group. Testosterone, FSH, Sperm cells count and Sperm activity (%) were significantly lower (p<0.05) in the male study group relative to the control group; while prolactin, S. cortisol and S. amylase were significantly higher (p<0.05) in the male study group relative to the control group. Conclusions: Abnormal hormones values and abnormal sperm quality and quantity are associated with elevated stress biomarkers in couples presenting with infertility. Strong positive correlations exist between hormones and stress biomarker in infertility conditions.

Assessing the efficacy of perioperative carprofen administration in dogs undergoing surgical repair of a ruptured cranial cruciate ligament

2000 ◽  
Vol 36 (5) ◽  
pp. 448-455 ◽  
Author(s):  
CJ Reese ◽  
EJ Trotter ◽  
CE Short ◽  
HN Erb ◽  
LL Barlow
Keyword(s):  
Body Weight ◽  
Postoperative Pain ◽  
Pain Score ◽  
Surgical Repair ◽  
Cruciate Ligament ◽  
Serum Cortisol ◽  
Cranial Cruciate Ligament ◽  
Significant Difference ◽  
Subjective Pain ◽  
Cortisol Levels

Twenty-one otherwise healthy dogs that presented for surgical repair of a ruptured cranial cruciate ligament were blindly and randomly given either carprofen (2.2 mg/kg body weight, orally) or a placebo beginning 12 hours preoperatively and continuing every 12 hours for a total of three doses. The patients were assessed for postoperative pain using a subjective pain score and given oxymorphone (0.1 mg/kg body weight, intramuscularly) every four hours if the pain score was 2 or greater. Blood samples were also collected to determine serum cortisol levels. There was a significant increase in serum cortisol levels in the immediate postoperative period in both the placebo group and the carprofen group (p less than 0.05). There was no significant difference in the percentage of increase in serum cortisol levels between the two groups. No correlation was evident between the serum cortisol levels and the corresponding pain scores in either group. This subjective method of assessing postoperative pain was not accurate and should not be relied upon for determination of postoperative analgesic administration. Perioperative oral administration of carprofen did not appear to be effective in controlling postoperative pain in these patients.

Salivary alpha amylase and cortisol levels in children with global developmental delay and their relation with the expectation of dental care and behavior during the intervention

2012 ◽  
Vol 33 (2) ◽  
pp. 499-505 ◽  
Author(s):  
Márcio José Possari dos Santos ◽  
Daniel Galera Bernabé ◽  
Ana Cláudia de Melo Stevanato Nakamune ◽  
Silvia Helena Venturoli Perri ◽  
Sandra Maria Herondina Coelho Ávila de Aguiar ◽  
...  
Keyword(s):  
Developmental Delay ◽  
Dental Care ◽  
Alpha Amylase ◽  
Global Developmental Delay ◽  
Salivary Alpha Amylase ◽  
Cortisol Levels ◽  
And Behavior

scholarly journals Different Types of Environmental Stressors Could Have Disruptive or Constructive Effects on Vestibular Compensation

2021 ◽  
Author(s):  
Sara Sardari ◽  
Majid Asadi-Shekaari ◽  
Mohammad Khaksari ◽  
Iraj Aghaei ◽  
Fateme Delavari ◽  
...  
Keyword(s):  
Open Field ◽  
Maternal Separation ◽  
Motor Coordination ◽  
Serum Cortisol ◽  
Vestibular Compensation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Intermittent Fasting ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Cortisol Levels

Background and Aim: Stress could play either helpful or harmful roles in vestibular compensation, the process of recovery after vestibular system lesions. Herein, we examined the effect of two stressor types on vestibular compensation: chronic anxiety disorder induced by early maternal separation (MS), and caloric restriction by an intermittent fasting (IF) diet. Methods: Male Wistar rats (n=56) received maternal separation (the MS group), intermittent fasting (IF group), unilateral vestibular deafferentation (UVD group), or a mixture of these interventions (UVD+IF, UVD+MS, and UVD+IF+MS). All the groups were compared with control animals. The animals’ balance, motor coordination, anxiety, locomotor activity, and serum cortisol levels were evaluated by rotarod, open field, and enzyme-linked immunosorbent assay methods, respectively. The data were compared with those of the healthy control (HC) group. Results: The UVD animals did not show a significant change in the time on the rod, except for the IF+UVD group (p=0.04). There was no significant difference between the experimental groups on the open field indices, except for the MS+IF+UVD group which traveled a significantly less total distance (p=0.02). Serum cortisol levels were significantly higher than HCs for all the groups except for the sham saline and IF+UVD group (p<0.05). Conclusion: IF seems to promote compensation after UVD, while MS may disrupt it. However, IF loses its beneficial outcomes if the animal has received another source of stress, i.e. MS.

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