Association between ambient particulate matter exposure and semen quality in fertile men

Abstract Background Several studies have suggested adverse effects of particulate matter (PM) exposure on male reproductive health; few have investigated the association between PM exposure and semen quality in a large population of fertile men. Methods We evaluated 14 parameters of semen quality in 1554 fertile men in Nanjing from 2014 to 2016. Individual exposure to particular matter ≤10 μm in diameter (PM10) and ≤ 2.5 μm in diameter (PM2.5) during key periods of sperm development (0-90, 0-9, 10-14, 15-69, and 70-90 days before semen collection) were estimated by inverse distance weighting interpolation. Associations between PM exposure and semen quality were estimated using multivariable linear regression. Results Higher 90-days average PM2.5 was in association with decreased sperm motility (2.21% for total motility, 1.93% for progressive motility per 10 μg/m3 increase, P < 0.001) and four quantitative aspects of sperm motion (curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), and amplitude of lateral head displacement (ALH), P < 0.01). The association between PM2.5 exposure and semen quality were generally stronger for the earlier exposure window (70-90 days prior to ejaculation) than for recent exposure (0-9, 10-14, or 15-69 days). In the subgroup of men who had normal sperm parameters (n = 1019), similar results were obtained. Ninety-days PM10 exposure was associated only with decreased VCL and VAP and was not related to sperm concentration. Conclusions Exposure to PM2.5 adversely affects semen quality, specifically lower sperm motility, in fertile men. Graphical abstract

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Correlation between the sperm characteristics evaluated by a computerized system (CASA) and the reproductive performance of sows

Research Society and Development ◽

10.33448/rsd-v9i6.3389 ◽

2020 ◽

Vol 9 (6) ◽

pp. e09963389

Author(s):

Emanuelle Maria Gottardi ◽

Thaisa Campos Marques ◽

Karen Martins Leão ◽

Francisco Ribeiro de Araújo Neto ◽

Leidiane Gonçalves Fernandes

Keyword(s):

Sperm Motility ◽

Semen Quality ◽

Semen Analysis ◽

Computer Assisted ◽

Head Displacement ◽

Sperm Characteristics ◽

Flagellar Beat ◽

Straight Line ◽

Computerized System ◽

Farrowing Rate

Computer-assisted semen analysis (CASA) systems have been one the most used tools to evaluate sperm kinetics. The objective of this research was to estimate the correlation between sperm motility characteristics evaluated by CASA during 72 hours of cooling with the farrowing rate (FR) and total number of piglets born (TNB) after artificial insemination. Multiparous sows (n=464) were inseminated with semen from seven boars (19.6 ± 1.3 ejaculates/male). Sperm motility parameters were determined immediately after dilution and after 24, 48 and 72 hours of cooling at 15°C: total motility (TM-%), progressive motility (PM-%), curvilinear velocity (VCL-μm/s), straight line velocity (VSL-μm/s), average path velocity (VAP-µm/s), amplitude of lateral head displacement (ALH-µm), flagellar beat cross frequency (BCF-Hz), straightness (STR-%) and linearity (LIN-%). Pearson’s correlation coefficient was applied to analyze the data and the comparison of the means of the sperm characteristics between the boars was done by Tukey’s test at 5% probability. TM and PM at time zero (T0) were significant and had a moderate to high correlation with FR and TNB. After 72 hours of refrigeration, the semen quality was reduced and showed a significant and low correlation of the TM and PM with these same parameters. The boar presenting the lowest value of TM and PM after dilution obtained lower FR and TNB. In conclusion, computer-assisted semen analysis soon after dilution can be used to predict fertility of boars.

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Semen quality and diversity of morphological sperm abnormalities in bulls: breed and strain effects

Vavilov Journal of Genetics and Breeding ◽

10.18699/vj18.435 ◽

2019 ◽

Vol 22 (8) ◽

pp. 931-938

Author(s):

M. A. Kleshchev ◽

V. L. Petukhov ◽

L. V. Osadchuk

Keyword(s):

Sperm Motility ◽

Environmental Conditions ◽

Western Siberia ◽

Semen Quality ◽

Sperm Count ◽

Sperm Concentration ◽

Sperm Head ◽

Sperm Dna Fragmentation ◽

Sperm Production ◽

Sperm Abnormalities

At present great attention is paid to studying genetic regulation of farm animal adaptations to environmental conditions. This problem is very important due to a wide expansion of highly productive cattle breeds created in Europe and North America. However, until the present no investigation of changing semen quality in bulls of imported breeds during their adaptations to environmental conditions of Western Siberia has been conducted. The aim of this study was to investigate semen quality peculiarities and the diversity of morphological sperm abnormalities in bulls of imported and local breeds kept in the environmental conditions of the southern part of Western Siberia. We determined sperm concentration, sperm count, and rate of sperm with progressive motility and percentage of morphologically normal spermatozoa. The rate of sperm abnormalities according to Blome’s classifcation was determined too. It was found that the mean values of sperm concentration, sperm motility and percentage of morphologically normal spermatozoa in the bulls investigated were similar to those in bulls kept in European countries. Interbreed diﬀerences in these parameters were not found. However, bulls of the Red Danish, Angler, and Simmental breeds had a higher percentage of misshapen sperm head and pyriform sperm head than bulls of the BlackWhite breed. An interstrain diﬀerence in sperm motility in bulls of the BlackWhite breed was observed. It was found that bulls of Reﬂection Sovereign 198998 strain had lover sperm motility than bulls of Wis Burke Ideal 1013415 strain. No interstrain diﬀerences in sperm production, percentage of morphologically normal spermatozoa and rate of main sperm abnormalities were found. Thus, it has been found that the environmental conditions of the southern part of Western Siberia do not seriously aﬀect the sperm production, sperm motility or percentage of morphologically normal spermatozoa in bulls. However, the increased rate of misshapen and pyriform sperm heads in the bulls of the foreign breeds points to a need to study sperm DNA fragmentation.

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23 Sperm quality of Pure Spanish stallions is affected by inbreeding coefficient and age

Reproduction Fertility and Development ◽

10.1071/rdv32n2ab23 ◽

2020 ◽

Vol 32 (2) ◽

pp. 137

Author(s):

Y. Pirosanto ◽

M. Valera ◽

A. Molina ◽

J. Dorado ◽

S. Demyda-Peyrás

Keyword(s):

Sperm Motility ◽

Inbreeding Depression ◽

Negative Correlation ◽

Semen Quality ◽

Sperm Quality ◽

Sperm Concentration ◽

Inbreeding Coefficient ◽

Semen Parameters ◽

Computer Assisted

Inbreeding depression, a genetic condition produced by the mating of close-related individuals, has been associated with a reduction of fertility in several species. However, a loss in sperm quality was also associated with age. In horses, the few existing reports have described a tendency of both parameters to produce a negative effect on sperm quality. However, those reports were performed using a subjective evaluation of sperm motility. In the present study, a total of 692 ejaculates from 86 Pure Spanish stallions (PRE), aged between 3 and 22 years, were evaluated using a computer-assisted methodology to determine the effect of inbreeding in four semen parameters: free-gel volume (V), sperm concentration (C, by haemocytometer), and total (TM) and progressive (PM) sperm motility (by Spermvision sperm class analyser; Minitube). The inbreeding coefficient (F) was estimated using 300 000 PRE pedigree records approximately (minimum pedigree depth, eight equivalent complete generations; range, between 1 and 30.1%). Stallion, age, ejaculate, and season of semen collection were the variables included in the statistical model (general linear model), with ejaculate and season being the variables with a major effect (by variance components analysis). Our results showed that sperm concentration (r=−0.18; P<0.0001) and volume (to a lesser extent) were reduced with advancing age, both showing a major decline after 15 years of age. To the contrary, sperm motility was not affected by age of the stallion. We also found a negative correlation between the inbreeding coefficient and ejaculate volume (r=−0.14; P<0.001), with a marked decrease seen when F was between 7 and 20%. Also, a negative correlation was observed in PM (r=−0.08; P<0.05), although to a lower extent. Conversely, C and TM were not affected by inbreeding depression (P>0.05). In conclusion, our results demonstrated that high levels of inbreeding can compromise severely the sperm quality of the PRE stallion, which, subsequently, may have a negative influence on fertility. Ongoing studies using genomic data will help to detect genetic variants associated with stallion semen quality and how it is influenced by inbreeding in specific genomic regions.

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Repeatability Estimates For Seminal Traits And Their Phenotypic Relationships With Testes Measurements And Performance Traits In Black Bengal Buck

Bangladesh Journal of Animal Science ◽

10.3329/bjas.v37i2.9879 ◽

1970 ◽

Vol 37 (2) ◽

pp. 34-41 ◽

Cited By ~ 1

Author(s):

AK Rajuana ◽

MR Tayabur ◽

MA Hoque ◽

SS Husain ◽

Z Sultana

Keyword(s):

Body Weight ◽

Sperm Motility ◽

Body Condition ◽

Semen Quality ◽

Sperm Concentration ◽

Normal Sperm ◽

Semen Volume ◽

Performance Traits ◽

Mass Activity ◽

And Performance

Repeatability for seminal traits and their phenotypic relationships with testes measurements and performance traits in Black Bengal bucks were estimated from a total of 116 repeated observations on 15 young Black Bengal bucks. Performance traits included age, body condition and body weight of bucks. Testes measurements were testes length, breadth and volume, and scrotal circumference, while seminal traits were ejaculate volume, semen density, mass activity, sperm motility, sperm concentration, total sperm per ejaculation and percent of normal sperm. High positive correlations (ranging from 0.81 to 0.90) were found between body weight and testes measurements. Semen volume and percent of normal sperm were positively correlated with age, body condition and body weight of bucks (ranging from 0.24 to 0.60). The testes measurements were strongly and positively correlated with semen volume and total sperm per ejaculation (ranging from 0.53 to 0.61), while the correlations between testes measurement and percent of normal sperm were moderate (ranging from 0.34 to 0.44). Among seminal traits, strong correlations were found between semen volume and total sperm per ejaculation (0.81) and, between mass activity and sperm motility (0.82). Repeatability of ejaculate volume was higher (r = 0.78) which indicated that selection or culling for semen ejaculate volume could be practiced from single or few observations. It could be concluded that bucks’ age, body weight and body condition along with testes volume should be considered as selection criteria for improving semen quality and semen production of breeding bucks. DOI: http://dx.doi.org/10.3329/bjas.v37i2.9879 BJAS 2008; 37(2): 34-41

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Efecto de la composición racial sobre la calidad espermática de verracos

Agronomía Mesoamericana ◽

10.15517/ma.v29i3.32445 ◽

2018 ◽

Vol 29 (3) ◽

pp. 485 ◽

Cited By ~ 2

Author(s):

Anthony Valverde-Abarca ◽

Mónica Madrigal-Valverde ◽

Marlen Camacho-Calvo ◽

Adones Zambrana-Jiménez ◽

Leonardo López

Keyword(s):

Quality Evaluation ◽

Semen Quality ◽

Sperm Quality ◽

Active Movement ◽

Racial Groups ◽

Swine Farms ◽

Progressive Motility ◽

Head Displacement ◽

Straight Line ◽

Lateral Head

The assessment of the semen quality is an essential tool to improve the reproductive indexes in swine farms. The motility is the most important parameter that used in the sperm quality evaluation because it is associated with the energy of the spermatozoon. The aim of this research was to determine the influence of breed composition on reproductive variables of sperm quality, kinetics and semen motility in boars. During 2016, 240 ejaculates were collected from 63 boars with a mean age of 24.4±10.9 months. Six racial groups were identified: Duroc (D), Yorkshire (Y), Landrace (L), F1 Pietrain*Duroc (PD) and two genetic lines (LA and LB). The breeds with the highest ejaculate volume presented a higher total number of spermatozoa (P<0.05). The effect of the breed was significant (P<0.05) on sperm kinetics variables except for amplitude of lateral head displacement (ALH, μm). The Landrace breed presented the highest (P<0.05) percentage of static sperm (29.30±1.57). The most relevant differences (P<0.05) for total motility (MTOT) and progressive motility (MP), were presented between L and PD with values of 70.71±1.57; 77.48±1.09 and 51.80±1.97; 59.85±1.37% respectively. Adult boars (≥18 months) had higher volumes of ejaculate and total number of sperm than boars in the intermediate and young ages, however, for velocities (μm/s): curvilinear (VCL), straight line (VSL) and average path (VAP), adult boars only were different (P<0.05) of the boars in-between ages. Four sperm subpopulations (SP) were identified, SP1 (46.83%) characterized by a moderate speed, but of very progressive motility, SP2 (14.78%) with active movement, but not progressive, SP3 (8.45%) with low speeds and progressive path and SP4 (29.94%) with quick movement, but without progressivity.

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Seasonal variations in quantitative and qualitative sperm characteristics in fertile and subfertile stallions

Archives Animal Breeding ◽

10.5194/aab-63-145-2020 ◽

2020 ◽

Vol 63 (1) ◽

pp. 145-154 ◽

Cited By ~ 1

Author(s):

Yara Suliman ◽

Frank Becker ◽

Armin Tuchscherer ◽

Klaus Wimmers

Keyword(s):

Breeding Season ◽

Seasonal Variations ◽

Semen Quality ◽

Semen Analysis ◽

Sperm Concentration ◽

Quality Parameters ◽

Computer Assisted ◽

Normal Sperm ◽

Straight Line ◽

Significant Seasonal Variation

Abstract. Horses are seasonal breeders with a natural breeding season beginning in spring and extending through midsummer. In this study, quantitative and qualitative parameters of chilled stallion semen were compared between fertile and subfertile stallions and between the breeding and the non-breeding season. Semen quality parameters compared included ejaculate volume, sperm concentration, total sperm number, sperm morphology, and computer-assisted semen analysis (CASA)-derived sperm movement characteristics obtained from two groups of warmblood stallions (n=8; four fertile stallions and four subfertile stallions), which differ in the seasonal pregnancy rate 80 %–90 % (fertile) vs. 40 %–60 % (subfertile). A total of 64 ejaculates were collected from the stallions (n=8; four in the breeding season and four in the non-breeding season of each stallion). No significant differences in the semen quality parameters between the fertile and the subfertile stallions in the non-breeding season were observed. However, in the breeding season the proportion of morphologically normal sperm, total motility, progressive motility, average path velocity (VAP), and curvilinear velocity (VCL) were significantly higher in the fertile group (P<0.05) when compared with the subfertile group. In addition, a significant seasonal variation in the proportion of morphological normal sperm was found in the fertile group between the breeding and the non-breeding season (P<0.05). Moreover, significant seasonal variations (P<0.05) in CASA parameters of mean VAP, straight line velocity (VSL), and beat-cross frequency (BCF) were observed in the fertile and the subfertile stallions, which tended to be lower in the non-breeding season. In conclusion, differences between the fertile and the subfertile stallions were observed only in the breeding season, and a few of CASA-derived parameters seemed to be significantly lower during the non-breeding season in both the fertile and the subfertile stallions.

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Raw Semen Characteristics of Three Different Indonesian Local Roosters

ANIMAL PRODUCTION ◽

10.20884/1.anprod.2016.18.3.555 ◽

2016 ◽

Vol 18 (3) ◽

pp. 165

Author(s):

Emilia Kamung Hambu ◽

Raden Iis Arifiantini ◽

Bambang Purwantara ◽

Sri Darwati

Keyword(s):

Sperm Motility ◽

Semen Quality ◽

Mass Movement ◽

Sperm Concentration ◽

Semen Volume ◽

Sperm Abnormality ◽

Microscopic Evaluation ◽

Semen Characteristics ◽

Live Sperm ◽

Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of Merawang roosters, Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of Merawang (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters. Sperm concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

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ASSESSMENT OF BOVINE SEMEN QUALITY BY RESAZURIN REDUCTION TEST

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/tijovsab.v12i1.11244 ◽

2016 ◽

Vol 12 (1) ◽

Author(s):

Umesh Kumar ◽

Prasanna Pati ◽

A.P. Gawande

Keyword(s):

Sperm Motility ◽

Semen Quality ◽

Sperm Concentration ◽

Mean Value ◽

Bovine Semen ◽

Reduction Test ◽

The Mean ◽

Acrosomal Integrity

A study was conducted to assess the semen quality using Resazurin Reduction Test (RRT) in Jersey and Jersey cross bulls. A total 36 fresh ejaculates were collected for evaluation RRT, sperm motility, acrosomal integrity and sperm concentration. The mean value of RRT in bulls was 15.77±0.85 minutes.Statistical analysis revealed that RRT was highly significantly (p<0.01) and positively correlated with sperm concentration (r=0.769), sperm motility(r=0.858) and acrosomal integrity (r=0.515), hence it may be helpful in evaluating the quality of semen in bulls.

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Computer assisted sperm analysis of fresh and frozen-thawed buffalo semen and their interrelationship

Indian Journal of Animal Research ◽

10.18805/ijar.8559 ◽

2016 ◽

Vol 50 (1) ◽

Author(s):

J. B. Patel ◽

A. J. Dhami

Keyword(s):

Sperm Motility ◽

Computer Assisted ◽

Motile Sperm ◽

Mean Values ◽

Sperm Analysis ◽

Head Displacement ◽

Straight Line ◽

Buffalo Semen ◽

Live Sperm ◽

Fresh Semen

Sixty semen ejaculates from 10 mature bulls, 5 each of Jafarabadi and Mehsana breed, were studied for sperm motility and velocity parameters of fresh and frozen-thawed spermatozoa using computer assisted sperm analyzer (CASA). The mean values of motile and progressively motile spermatozoa observed in fresh semen of Jafarabadi and Mehsana bulls (79.77±1.62 and 61.80±1.85, and 78.90±1.22 and 61.37±1.58%) were highly significantly (P<0.01) reduced (51.20±1.57 and 33.20±1.45, and 52.10±1.70 and 34.30±1.54 %, respectively) in post-thawed semen. The average path velocity, straight line velocity and curvilinear velocity (μm/sec) of spermatozoa of Jafarabadi and Mehsana bulls noted in fresh semen were also reduced highly significantly (P<0.01) in frozen-thawed semen. Among the other velocity parameters, amplitude of lateral head displacement (μm), elongation (%) and medium motile sperm (%) increased, while beat-cross frequency (Hz), straightness (%), linearity (%), sperm area (μm<sup>2</sup>) and rapidly motile sperm (%) decreased significantly in post-thawed sperms when compared with the fresh sperm of both Jafarabadi and Mehsana bulls. The initial motility and live sperm per cent were significantly correlated with CASA traits of fresh and frozen-thawed semen, and all the sperm motility and velocity traits of fresh and frozen-thawed semen assessed by CASA were significantly interrelated among both the breeds. The interrelationships were stronger in Mehsana bulls as compared to Jafarabadi bulls.

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