scholarly journals Antimicrobial and antibiofilm activities of Cu(II) Schiff base complexes against methicillin-susceptible and resistant Staphylococcus aureus

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Pooi Yin Chung ◽  
Ranon Earn Yueh Khoo ◽  
Hui Shan Liew ◽  
May Lee Low
Keyword(s):  
Staphylococcus Aureus ◽  
Schiff Base ◽  
Cell Line ◽  
Copper Complexes ◽  
Methicillin Resistance ◽  
Schiff Base Complexes ◽  
Normal Lung ◽  
Antibiofilm Activity ◽  
Drug Of Choice ◽  
The Individual

Abstract Background Methicillin-resistance S. aureus (MRSA) possesses the ability to resist multiple antibiotics and form biofilm. Currently, vancomycin remains the last drug of choice for treatment of MRSA infection. The emergence of vancomycin-resistant S. aureus (VRSA) has necessitated the development of new therapeutic agents against MRSA. In this study, the antimicrobial and antibiofilm activities of two copper-complexes derived from Schiff base (SBDs) were tested individually, and in combination with oxacillin (OXA) and vancomycin (VAN) against reference strains methicillin-susceptible and methicillin-resistant Staphylococcus aureus. The toxicity of the SBDs was also evaluated on a non-cancerous mammalian cell line. Methods The antimicrobial activity was tested against the planktonic S. aureus cells using the microdilution broth assay, while the antibiofilm activity were evaluated using the crystal violet and resazurin assays. The cytotoxicity of the SBDs was assessed on MRC5 (normal lung tissue), using the MTT assay. Results The individual SBDs showed significant reduction of biomass and metabolic activity in both S. aureus strains. Combinations of the SBDs with OXA and VAN were mainly additive against the planktonic cells and cells in the biofilm. Both the compounds showed moderate toxicity against the MRC5 cell line. The selectivity index suggested that the compounds were more cytotoxic to S. aureus than the normal cells. Conclusion Both the SBD compounds demonstrated promising antimicrobial and antibiofilm activities and have the potential to be further developed as an antimicrobial agent against infections caused by MRSA.

Treatment of Infection and Colonization Caused by Methicillin-Resistant Staphylococcus aureus

1991 ◽  
Vol 12 (01) ◽  
pp. 29-35 ◽  
Author(s):  
Henry F. Chambers
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Active Agent ◽  
Methicillin Resistant ◽  
Drug Of Choice ◽  
Serious Infections ◽  
Combination Regimens ◽  
Higher Doses

AbstractThe mechanism of methicillin resistance confers resistance to all available B-lactam antibiotics; consequently, B-lactam antibiotics have no role in therapy of methicillin-resistant Staphylococcus aureus (MRSA) infections. Vancomycin remains the drug of choice. Teicoplanin and daptomycin are two investigational antibiotics related to vancomycin in structure and in spectrum of activity. In clinical trials employing relatively low doses, neither was as effective as vancomycin. Trials at higher doses are on-going. Quinolones, ciprofloxacin in particular, have been used successfully to treat infections caused by MRSA; however, the usefulness of quinolones may be limited by the tendency of resistance to emerge during therapy. Quinolones probably should be used only in combination with another active agent, such as rifampin, when treating serious infections caused by MRSA. Other agents may be active in vitro against MRSA, but clinical data showing their effectiveness are lacking. Rifampin combination regimens appear most effectively to eradicate colonization with MRSA.

scholarly journals (E)3-2-(1-(2,4-Dihydroxyphenyl)ethyldeneamino)phenyl)-2-methylquinazoline-4(3H)-one Schiff Base and Its Metal Complexes: A New Drug of Choice against Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
K. Siddappa ◽  
Sunilkumar B. Mane ◽  
Deene Manikprabhu
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Schiff Base ◽  
Metal Complexes ◽  
Methicillin Resistant ◽  
New Drug ◽  
Drug Of Choice

The 3-(2-aminophenyl) quinazolin-2-methyl-4(3H)-one and 2,4-dihydroxyacetophenone undergo condensation to afford (E)3-2-(1-(2,4-dihydroxyphenyl)ethyldeneamino)phenyl)-2-methylquinazoline-4(3H)-one Schiff base (DHPEAPMQ). The newly synthesized Schiff base (DHPEAPMQ) and its metal complexes were evaluated for their antimicrobial activity against methicillin-resistantStaphylococcus aureusisolated from the Gulbarga region in India. The Cu(II), Ni(II), and Zn(II) complexes of Schiff base (DHPEAPMQ) showed good antimicrobial activity. So, this could be a new drug of choice.

scholarly journals Synthesis, crystal structures and spectroscopic investigation of new Cu/Schiff-base complexes

2014 ◽  
Vol 10 (8) ◽  
pp. 3068-3079
Author(s):  
Ahmed Toumi ◽  
Mohamed Rzaigui ◽  
Hichem Ben Jannet
Keyword(s):  
Schiff Base ◽  
Solid State ◽  
Copper Complexes ◽  
Spectroscopic Properties ◽  
Schiff Base Complexes ◽  
X Ray Diffraction ◽  
Neutral Complex ◽  
Schiff Base Ligands ◽  
1H And 13C Nmr ◽  
Solid State Structures

Three novel Copper complexes, [Cu(L1)2][CuCl2] (1),  [Cu(L2)Cl] (2) and [Cu2(L3)3Cl2] (3), have been prepared by reaction of CuCl with the Schiff-base ligands L1: N,N’-bis(thiophen-2-ylmethylene)-ethane-1,2-diamine, L2: N,N’-bis(1H-pyrrol-2-ylmethylene)ethane-1,2-diamine and L3: N,N’-bis(2-nitrobenzylidene)-ethane-1,2-diamine in acetonitrile. The solid-state structures of these complexes were determined by X-ray diffraction from single crystal data and characterized by 1H and 13C NMR, IR and UV/Vis spectroscopies. This study shows that (1) is an ionic complex with a Cu(I)-centered cation and an isolated linear dichlorocuprate(I) anion, (3) is a dinuclear neutral complex of Cu(I) while (2)  is a mononuclear neutral complex of Cu(II). In the three complexes, Cu is tetracordinated in different geometrical environments. The atomic arrangements and spectroscopic properties of the three complexes are reported. Complexes 1-3 exhibit, in the solid state at room temperature, photoluminescence between 320 and 550 nm.

scholarly journals One Pot Synthesis of Schiff Base Complexes of Cu(II), Zn(II), Ni(II), Co(II) and their Cytotoxicity, Molecular Docking and Antibacterial Activity

2020 ◽  
Vol 32 (10) ◽  
pp. 2507-2514
Author(s):  
A. Ashma ◽  
A. Sudha ◽  
S.L. Ashok Kumar ◽  
G. Sasikumar ◽  
S.J. Askar Ali
Keyword(s):  
Schiff Base ◽  
Cell Line ◽  
Atomic Ratio ◽  
Schiff Base Complexes ◽  
One Pot ◽  
Human Carcinoma ◽  
Vis Spectroscopy ◽  
Ft Ir ◽  
Cancer Activity

A novel Schiff base ligand (L), N(4)-cyclohexyl-2-(1-(5-chlorothiophen-2-yl)ethylidene)hydrazine carbothioamide was synthesized from isothiocyanatocyclohexane and hydrazine. All the synthesized transition metal(II) complexes were characterized by functional peak using 1H NMR, 13C NMR, FT-IR, UV-vis spectroscopy and metal complex atomic ratio of complexes of thiosemicarbazones were characterized by elemental analysis. Schiff base and its metal(II) complexes were screened in vitro medicinal drug activity against Gram positive and Gram-negative microorganisms and anti-cancer activity against human carcinoma cell line MCF-7 was detected with nickel(II) and cobalt(II) complexes. Further, the cytotoxity of ligand and its metal(II) complexes were tested on a Monkeys normal cell line and found to be non-toxic (< 200 μg/mL). In the present work, the molecular mechanism of anticancer (breast cancer) activity of the compounds were also disscussed.

Binuclear complexes of copper(II) and zinc(II) halides with bidentate and quadridentate Schiff base complexes

1968 ◽  
Vol 21 (6) ◽  
pp. 1473 ◽  
Author(s):  
GE Batley ◽  
DP Graddon
Keyword(s):  
Schiff Base ◽  
Copper Complexes ◽  
Solvent Molecule ◽  
Schiff Base Complexes ◽  
Binuclear Complexes ◽  
Phenolic Oxygen ◽  
Near Ultraviolet ◽  
Tetrahedral Environment ◽  
Binuclear Structure ◽  
Solvent Molecules

Binuclear complexes of the types CuB,CuCl2, CuB,ZnCl2, ZnB,ZnCl2, and CuL2,CuCl2 have been obtained where BH2 is a quadridentate Schiff base derived from salicylaldehyde and a polyrnethylenediamine and LH is an N-alkylsalicylaldimine. The stereochemistry of the copper atoms in the binuclear complexes is inferred from comparisons of their reflectance spectra with the spectra of the simple copper complexes CuB and CuL2. The structures of the zinc complexes are discussed by reference to near ultraviolet spectra of the ligands and infrared spectra in the 1500-1560 cm-1 region. The evidence suggests that in the complexes of the type MB,M'Cl2 either but not both metal atoms may approach a tetrahedral environment. The chemistry of the complexes ZnB,ZnCl2 is complicated by the possibility of either or both zinc atoms becoming 5 coordinate by addition of solvent molecules; in some of these complexes the zinc chloride appears to be attached to a solvent molecule in a 5-coordinate Schiff base unit, rather than by bridging through phenolic oxygen atoms. The oomplexes CuL2,CuCl2 apparently have a symmetrical binuclear structure based on a trans arrangement of the Schiff base units rather than an unsymmetrical structure resembling that of the complexes CuB,CuCl2. Complexes of the types CuL2,ZnCl2 and ZnL2,ZnCl2 could not be obtained.

Molecular Characterization of Methicillin Resistant and Extended Spectrum β-Lactamase Staphylococcus aureus Isolated from Burn Patients

2020 ◽  
pp. 145-151
Author(s):  
Shawnm Ahmed Aziz
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Vancomycin Resistance ◽  
World Health ◽  
Molecular Technique ◽  
Burn Patients ◽  
Beta Lactamase ◽  
Beta Lactam ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

Antibiotic resistance has become a major world health challenge and has limited the ability of physician's treatment. Staphylococcus aureus the most notorious pathogens causes morbidity and mortality especially in burn patients. However, Staphylococcus aureus rapidly acquired resistance to multiple antibiotics. Vancomycin, a glycopeptide antibiotic remains a drug of choice for treatment of severe Methicillin Resistance S. aureus infections. This study aimed to detect the emergence of beta-lactam and glycopeptide resistance genes. 50 clinical specimens of S. aureus collected from burn patients in burn and plastic surgery units in Sulaimani-Iraq city. All specimens were confirmed to be positive for S. aureus. All the isolates were assessed for their susceptibility to different antibiotics depending on NCCL standards, followed by Extended Spectrum Beta Lactamase detection by double disk diffusion synergy test. The production of β- lactamases was evaluated in the isolated strains by several routine methods and polymerase chain reaction. Among the isolates 94% were Methicillin resistance and 34.28% were Extended Spectrum Beta Lactamase producer. PCR based molecular technique was done for the bla genes related to β- lactamase enzymes by the specific primers, as well as genes which related to reduced sensitivity to Vancomycin were detected. The results indicated that all isolated showed the PBP1, PBP2, PBP3, PBP4, trfA and trfB, graSR, vraS except the vraR gene and the prolonged therapy of Methicillin resistance infection with teicoplanin have been associated with progress of resistance and the rise of tecoplanin resistance may be a prologue to evolving Vancomycin resistance. In conclusion, beta-lactam over taking can rise Vancomycin- Intermediate S. aureus strains leading to appearance of Vancomycin resistance although the treatment of Vancomycin resistant infections is challenging.

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