Significant co-expression of putative cancer stem cell markers, EpCAM and CD166, correlates with tumor stage and invasive behavior in colorectal cancer

Abstract Background The crucial oncogenic role of cancer stem cells (CSCs) in tumor maintenance, progression, drug resistance, and relapse has been clarified in different cancers, particularly in colorectal cancer (CRC). The current study was conducted to evaluate the co-expression pattern and clinical significance of epithelial cell adhesion molecules (EpCAM) and activated leukocyte cell adhesion (CD166 or ALCAM) in CRC patients. Methods This study was carried out on 458 paraffin-embedded CRC specimens by immunohistochemistry on tissue microarray (TMA) slides. Results Elevated expression of EpCAM and CD166 was observed in 61.5% (246/427) and 40.5% (164/405) of CRC cases. Our analysis showed a significant positive association of EpCAM expression with tumor size (P = 0.02), tumor stage (P = 0.007), tumor differentiate (P = 0.005), vascular (P = 0.01), neural (P = 0.01), and lymph node (P = 0.001) invasion. There were no significant differences between CD166 expression and clinicopathological parameters. Moreover, the combined analysis demonstrated a reciprocal significant correlation between EpCAM and CD166 expression (P = 0.02). Interestingly, there was a significant positive correlation between EpCAM/CD166 phenotypes expression and tumor stage (P = 0.03), tumor differentiation (P = 0.05), neural, and lymph node invasion (P =0.01). Conclusions The significant correlation of EpCAM and CD166 expression and their association with tumor progression and aggressive behavior is the reason for the suggestion of these two CSC markers as promising targets to promote novel effective targeted-therapy strategies for cancer treatment in the present study.

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Osteopontin Overexpression Induced Tumor Progression and Chemoresistance to Oxaliplatin through Induction of Stem-Like Properties in Human Colorectal Cancer

Stem Cells International ◽

10.1155/2015/247892 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 18

Author(s):

Lui Ng ◽

Timothy Wan ◽

Ariel Chow ◽

Deepak Iyer ◽

Johnny Man ◽

...

Keyword(s):

Colorectal Cancer ◽

Stem Cell ◽

Poor Prognosis ◽

Cancer Metastasis ◽

Tumor Stage ◽

Clinicopathological Parameters ◽

Stem Cell Markers ◽

Cell Markers ◽

Response To Chemotherapy

Colorectal cancer (CRC) is one of the most common and fatal malignancies worldwide. The poor prognosis of colorectal cancer patients is due to development of chemoresistance and cancer metastasis. Recently osteopontin (OPN) has been associated with stem-like properties in colorectal cancer. This study further examined the clinicopathological significance of OPN in CRC and its effect on chemoresistance and transcription of stem cell markers. We examined the transcription level of OPN in 84 CRC patients and correlated the expression with their clinicopathological parameters. The associations of OPN overexpression with transcription of stem cell markers and response to chemotherapy in DLD1-OPN overexpressing clones and CRC patients were also investigated. Our results showed that OPN was significantly overexpressed in CRC, and its overexpression correlated with tumor stage and poor prognosis. Overexpression of CRC induced OCT4 and SOX2 expressionin vitroand correlated with SOX2 overexpression in CRC patients. In addition, DLD1-OPN overexpressing cells showed enhanced ability to survive upon oxaliplatin treatment, and OPN expression was higher in CRC patients who were resistant to oxaliplatin-involved chemotherapy treatment. Thus, CRC cells overexpressing OPN demonstrated stem-like properties and OPN inhibition is a potential therapeutic approach to combat CRC progression and chemoresistance.

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Faculty Opinions recommendation of ILK Expression in Colorectal Cancer Is Associated with EMT, Cancer Stem Cell Markers and Chemoresistance.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.733254991.793550602 ◽

2018 ◽

Author(s):

Avri Ben-Ze'ev

Keyword(s):

Colorectal Cancer ◽

Stem Cell ◽

Cancer Stem Cell ◽

Stem Cell Markers ◽

Cell Markers ◽

Cancer Stem Cell Markers

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Cancer Stem Cell Markers, CD44 and ALDH1, for Assessment of Cancer Risk in OPMDs and Lymph Node Metastasis in Oral Squamous Cell Carcinoma

Head and Neck Pathology ◽

10.1007/s12105-021-01384-8 ◽

2021 ◽

Author(s):

Snehal Navnath Dhumal ◽

Sheetal Korde Choudhari ◽

Sangeeta Patankar ◽

Shrikrishna S. Ghule ◽

Yogesh B. Jadhav ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Stem Cell ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Oral Squamous Cell Carcinoma ◽

Cancer Risk ◽

Cancer Stem Cell ◽

Stem Cell Markers ◽

Cancer Stem Cell Markers ◽

Node Metastasis

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Prognostic impact of the expression of putative cancer stem cell markers CD133, CD166, CD44s, EpCAM, and ALDH1 in colorectal cancer

British Journal of Cancer ◽

10.1038/sj.bjc.6605762 ◽

2010 ◽

Vol 103 (3) ◽

pp. 382-390 ◽

Cited By ~ 167

Author(s):

A Lugli ◽

G Iezzi ◽

I Hostettler ◽

M G Muraro ◽

V Mele ◽

...

Keyword(s):

Colorectal Cancer ◽

Stem Cell ◽

Cancer Stem Cell ◽

Prognostic Impact ◽

Stem Cell Markers ◽

Cell Markers ◽

Cancer Stem Cell Markers

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The Correlation between PSCA Expression and Neuroendocrine Differentiation in Prostate Cancer

BioMed Research International ◽

10.1155/2020/5395312 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Qian Xiang ◽

Zhiguo Zhu ◽

Lianmin Luo ◽

Jiamin Wang ◽

Yangzhou Liu ◽

...

Keyword(s):

Prostate Cancer ◽

Cancer Patients ◽

Specific Antigen ◽

Neuroendocrine Differentiation ◽

Positive Association ◽

Tumor Stage ◽

Clinicopathological Parameters ◽

Specific Marker ◽

Castration Resistant Prostate Cancer ◽

Neuroendocrine Prostate Cancer

The prostate stem cell antigen (PSCA), as a predominantly prostate-specific marker, is overexpressed in most prostate cancer specimens, is positively correlated with prostate cancer androgen independence, and has the potential to be treated with castration-resistant prostate cancer (CRPC) as a gene therapy target. Using the typical androgen deprivation therapy, most tumors will progress to CRPC, as well as develop into neuroendocrine prostate cancer (NEPC) characterized by the expression of neuroendocrine markers such as enolase 2 (NSE). Our study was aimed at investigating the expressions of PSCA and NSE and the relationship between the two markers, as well as the correlation between the PSCA and NSE expressions and the clinicopathological parameters in prostate cancer specimens from 118 patients by using immunohistochemistry. Our results demonstrated that the PSCA and NSE protein expressions did not correlate with the prostate cancer patients’ age or the hormone therapy but showed a significant correlation with the pathological tumor stage of prostate cancer, the Gleason score, and the presence of metastasis. There is a positive association between PSCA and NSE but a negative one between the prostate-specific antigen (PSA) and PSCA or between PSA and NSE. High PSCA and NSE expressions correlated with a poor prognosis in prostate cancer patients. PSCA may play an important role in the progression of neuroendocrine prostate cancer (NEPC).

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Rationales for the Use of Cancer Stem Cells Markers in the Staging of Papillary Thyroid Carcinoma

Journal of Oncology ◽

10.1155/2019/1659654 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Noah Abd-Alkader Mahmood ◽

Amer Talib Tawfeeq ◽

Israa Mhdi Al-Sudani ◽

Zaynab Saad Abd-Alghni

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Histopathological Examination ◽

Standard Procedure ◽

Tumor Stage ◽

Needle Aspiration ◽

Stem Cell Markers ◽

Papillary Thyroid ◽

Expression Levels ◽

Cancer Stem Cell Markers

Fine needle aspiration biopsy (FNAB) is a standard procedure for the detection of thyroid nodules malignancy, yet 10-25% of the sample diagnosed may go undetermined or suspicious. The utility of cancer stem cell markers (CSCM) as a differential diagnosis molecular marker in nodules of suspicious decision in FNAB was hypothesized. Papillary thyroid carcinoma (PTC) and thyroid fibroadenoma (TFA) samples were selected to test the hypothesis. The samples employed in this study were from patients who had thyroid hyperplasia and a suspicious or undetermined diagnosis by FNAB. The patient underwent a successful thyroidectomy at Al-Yarmouk Teaching Hospital in Baghdad between January 2015 and December 2017. All nodule samples underwent a systematic histopathological examination after resection. Tumors diagnosed as PTC and those diagnosed as fibroadenoma (TFA) were selected for this study. Collectively 39 PTC and 11 TFA nodules were included. Quantitative reverse transcriptase real-time PCR (qRT-PCR) and immunohistochemistry (IHC) were used to determine levels of mRNA and proteins of CSCM ALDH1A1, CD44, ABCG2, and Oct3/4 in both types of tumors were used. This study revealed that the expression levels of CSCM were significantly increased in PTC tissues when compared to benign tissues and the positive correlation was found between the CSCM expression levels and tumor stage, size, and gender. In conclusion, for a more precise diagnosis, we suggest these markers be included in what is currently available to characterize malignancy from what is not in thyroid cancer, as well as for the staging process of PTC.

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