scholarly journals Optimization of SPME–GC–MS and characterization of floral scents from Aquilegia japonica and A. amurensis flowers

BMC Chemistry ◽  
2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Hua-Ying Wang ◽  
Wei Zhang ◽  
Jian-Hua Dong ◽  
Hao Wu ◽  
Yuan-Hong Wang ◽  
...  
Keyword(s):  
Solid Phase ◽  
Rapid Evolution ◽  
Model Species ◽  
Floral Scents ◽  
Future Studies ◽  
Volatile Organic ◽  
New Ideas ◽  
Fiber Coatings

Abstract Background The floral scents of plants play a key role in plant reproduction through the communication between plants and pollinators. Aquilegia as a model species for studying evolution, however, there have been few studies on the floral scents and relationships between floral scents and pollination for Aquilegia taxa. Methods In this study, three types of solid-phase micro-extraction (SPME) fiber coatings (DVB/PDMS, CAR/PDMS, DVB/CAR/PDMS) were evaluated for their performance in extracting volatile organic compounds (VOCs) from flowers of Aquilegia amurensis, which can contribute to the future studies of elucidating the role of floral scents in the pollination process. Results In total, 55 VOCs were identified, and among them, 50, 47 and 45 VOCs were extracted by the DVB/CAR/PDMS fiber, CAR/PDMS fiber and DVB/PDMS fibers, respectively. Only 30 VOCs were detected in A. japonica taxa. Furthermore, the relative contents of 8 VOCs were significant different (VIP > 1 and p < 0.05) between the A. amurensis and A. japonica. Conclusions The results can be applied in new studies of the relationships between the chemical composition of floral scents and the processes of attraction of pollinator. It may provide new ideas for rapid evolution and frequent interspecific hybridization of Aquilegia.

scholarly journals Optimization of SPME-GC-MS and Characterization of Floral Scents from Aquilegia Japonica and A. Amurensis Flowers

2020 ◽  
Author(s):  
Hua-Ying Wang ◽  
Wei Zhang ◽  
Jian-Hua Dong ◽  
Hao Wu ◽  
Yuan-Hong Wang ◽  
...  
Keyword(s):  
Solid Phase ◽  
Floral Scent ◽  
Rapid Evolution ◽  
Model Species ◽  
Floral Scents ◽  
Future Studies ◽  
Volatile Organic ◽  
New Ideas

Abstract The floral scent of plants plays a key role in plant reproduction through the communication between plants and pollinators. Aquilegia as a model species for studying evolution, however, there have been few studies on the floral scents and relationships between floral scents and pollination for Aquilegia taxa. In this study, three types of solid-phase micro-extraction (SPME) fiber coatings (DVB/PDMS, CAR/PDMS, DVB/CAR/PDMS) were evaluated for their performance in extracting volatile organic compounds (VOCs) from flowers of Aquilegia amurensis, which can to contribute to the future studies of elucidating the role of floral scents in the pollination process. In total, 55 VOCs were identified, and among them, 50, 47 and 45 VOCs were extracted by the DVB/CAR/PDMS fiber, CAR/PDMS fiber and DVB/PDMS fibers, respectively. Only 30 VOCs were detected in A. japonica taxa. Furthermore, the relative contents of 8 VOCs were significant different (VIP > 1 and p < 0.05) between the A. amurensis and A. japonica. Therefore, the results can be applied in new studies of the relationships between the chemical composition of floral scents and the processes of attraction of pollinator. It may provide new ideas for rapid evolution and frequent interspecific hybridization of Aquilegia

scholarly journals Characterization of Volatile Organic Compounds in Mango Ginger (Curcuma amada Roxb.) from Myanmar

Metabolites ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Yanhang Chen ◽  
Musavvara Kh. Shukurova ◽  
Yonathan Asikin ◽  
Miyako Kusano ◽  
Kazuo N. Watanabe
Keyword(s):  
Solid Phase ◽  
Species Variation ◽  
Mango Ginger ◽  
Food Industries ◽  
Curcuma Amada ◽  
Alternative Medicines ◽  
Flight Mass Spectrometry ◽  
Volatile Organic ◽  
Loss Of Appetite

Curcuma amada Roxb. (Zingiberaceae), commonly known as mango ginger because its rhizome and foliar parts have a similar aroma to mango. The rhizome has been widely used in food industries and alternative medicines to treat a variety of internal diseases such as cough, bronchitis, indigestion, colic, loss of appetite, hiccups, and constipation. The composition of the volatile constituents in a fresh rhizome of C. amada is not reported in detail. The present study aimed to screen and characterize the composition of volatile organic compound (VOC) in a fresh rhizome of three C. amada (ZO45, ZO89, and ZO114) and one C. longa (ZO138) accessions originated from Myanmar. The analysis was carried out by means of headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-time-of-flight-mass spectrometry (GC-TOF-MS). As a result, 122 VOCs were tentatively identified from the extracted 373 mass spectra. The following compounds were the ten most highly abundant and broadly present ones: ar-turmerone, α-zingiberene, α-santalene, (E)-γ-atlantone, cuparene, β-bisabolene, teresantalol, β-sesquiphellandrene, trans-α-bergamotene, γ-curcumene. The intensity of ar-turmerone, the sesquiterpene which is mainly characterized in C. longa essential oil (up to 15.5–27.5%), was significantly higher in C. amada accession ZO89 (15.707 ± 5.78a) compared to C. longa accession ZO138 (0.300 ± 0.08b). Cis-α-bergamotene was not detected in two C. amada accessions ZO45 and ZO89. The study revealed between-species variation regarding identified VOCs in the fresh rhizome of C. amada and C. longa.

scholarly journals Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers

2018 ◽  
Vol 81 (8) ◽  
pp. 1231-1239 ◽  
Author(s):  
Francisca Aliny Nunes Silva ◽  
Alexander Alves da Silva ◽  
Nayanny de Sousa Fernandes ◽  
Tigressa Helena Soares Rodrigues ◽  
Kirley Marques Canuto ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Organic Compounds ◽  
Cucumis Melo ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Organic ◽  
Cucumis Melo L

scholarly journals Analysis of the Volatile Components of Pouteria sapota (Sapote Mamey) Fruit by HS-SPME-GC-MS

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Candelario Rodríguez ◽  
Armando A. Durant-Archibold ◽  
Ana Santana ◽  
Enrique Murillo ◽  
Carlos M. Franco Abuín
Keyword(s):  
Aromatic Hydrocarbons ◽  
Solid Phase Microextraction ◽  
Extraction Efficiency ◽  
Solid Phase ◽  
Volatile Components ◽  
Spme Fiber ◽  
Volatile Organic ◽  
Doehlert Experimental Design ◽  
First Time ◽  
Fiber Coatings

The comparison of SPME fiber coatings, and optimization of temperature and time of extraction for headspace solid phase microextraction (HS-SPME) of volatile organic compounds (VOCs) present in Pouteria sapota (sapote mamey) fruits is presented. The PDMS/DVB coating afforded the highest extraction efficiency. The extraction conditions were optimized by using Doehlert experimental design. By using the optimized HS-SPME method, 21 VOCs were identified, which include mainly terpenoids and esters, followed by aromatic hydrocarbons, aldehydes, alcohols and ketones. The most abundant compounds in Pouteria sapota pulp were cedrol (25.0%), azulene (7.3%), β-ionone (5.7%) naphthalene (5.6%), α-pinene (5.0%), and benzaldehyde (4.3%). Seventeen VOCs were identified for the first time in the fruit.

scholarly journals Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism

2020 ◽  
Author(s):  
Robert J. Nichols ◽  
Benjamin LaFrance ◽  
Naiya R. Phillips ◽  
Luke M. Oltrogge ◽  
Luis E. Valentin-Alvarado ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Biochemical Characterization ◽  
Sulfur Metabolism ◽  
Physiological Role ◽  
Enzymatic Characterization ◽  
Cysteine Desulfurase ◽  
Future Studies ◽  
Pcc 7942

AbstractProkaryotic nanocompartments, also known as encapsulins, are a recently discovered proteinaceous organelle in prokaryotes that compartmentalize cargo enzymes. While initial studies have begun to elucidate the structure and physiological roles of encapsulins, bioinformatic evidence suggests that a great diversity of encapsulin nanocompartments remains unexplored. Here, we describe a novel encapsulin in the freshwater cyanobacterium Synechococcus elongatus PCC 7942. This nanocompartment is upregulated upon sulfate starvation and encapsulates a cysteine desulfurase enzyme via an N-terminal targeting sequence. Using cryoelectron microscopy, we have determined the structure of the nanocompartment complex to 2.2 Å resolution. Lastly, biochemical characterization of the complex demonstrated that the activity of the cysteine desulfurase is enhanced upon encapsulation. Taken together, our discovery, structural analysis, and enzymatic characterization of this prokaryotic nanocompartment provide a foundation for future studies seeking to understand the physiological role of this encapsulin in various bacteria.

scholarly journals Molecular Identification and VOMs Characterization of Saccharomyces cerevisiae Strains Isolated from Madeira Region Winery Environments

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1058 ◽  
Author(s):  
Mariangie Castillo ◽  
Emanuel da Silva ◽  
José S. Câmara ◽  
Mahnaz Khadem
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Solid Phase ◽  
Rflp Analysis ◽  
Selective Adaptation ◽  
Internal Transcribed Spacers ◽  
Gas Chromatography Mass Spectrometry ◽  
Pcr Rflp ◽  
Volatile Organic ◽  
Major Chemical

The quality and typical characteristic of wines depends, among other factors, on the volatile organic metabolites (VOMs) that are biosynthesized by yeasts, mainly Saccharomyces cerevisiae species. The yeast strain influences the diversity and proportions of the VOMs produced during the fermentation process, as the genetic predisposition of the strains is a by-product of selective adaptation to the ecosystem. The present work reports the characterization of S. cerevisiae strains isolated from grape must, used in the Demarcated Region of Madeira (DRM) for winemaking. Yeast species were identified by amplification and by restriction fragment length polymorphism (RFLP) analysis of the region 5.8S-internal transcribed spacers (PCR-RFLP of 5.8S-ITS) of ribosomal DNA (rDNA). The strains identification was performed by analyzing the RFLP pattern of mitochondrial DNA (RFLP-mtDNA). The representative strains were selected for the characterization of the volatile profile through headspace solid-phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS) analysis. A total of 77 VOMs were identified. Higher alcohols, esters, and fatty acids were the major chemical families representing 63%, 16%, and 9%, respectively, in strain A and 54%, 23%, and 15% in strain B. The results indicate the influence of the strain metabolism in the production of VOMs, many of which probably participate in the aroma of the corresponding wines.

scholarly journals Paradigms and Paradoxes in the Metamorphosis of Ecotourism

2021 ◽  
Vol 40 (4) ◽  
pp. 71-84
Author(s):  
Loredana Dragomir ◽  
Mirela Mazilu
Keyword(s):  
Rapid Evolution ◽  
Research Problem ◽  
Human Society ◽  
Paradigm Shifts ◽  
Knowing That ◽  
New Concepts ◽  
New Ideas ◽  
Economic Reasoning

Abstract In 2020 and 2021, the entire evolution of human society is under the sign of a paradox, of the adversity of events, coming in avalanche. The tourist evolutions themselves suffer the imprint of the paradox. These paradoxes urgently require new paradigms, the famous ‘paradigm shifts’, mentality, optics, action. The purpose of this research is to outline the main aspects of the research problem and diagnose the situation, with focus on identifying hypotheses for future descriptive or causal research as well as to explore the reasons, attitudes and values of the paradigm and paradox, which differentiate the two notions approached: testing new concepts of forecasting, a product specific to the destination under analysis and in identifying other viable, sustainable alternatives and their analysis in parallel with modelling and promoting new ideas of tourism products or services, respectively improving the existing ones. This article aims to capitalise on the paradox, already successfully applied in economics by the author of the method and in shaping and delimiting ecotourism (in particular the ecotourism from the destination Ţara Haţegului – Retezat), emphasising the role of self-contradiction of the field, through a specific type of economic reasoning, in which the rapid evolution of tourism risks are becoming its own cause of its disappearance, knowing that too much tourism kills tourism. Responsibility and the mesological spirit are the only ways to counteract the paradox phenomenon, even a paradigm in the metamorphosis of ecotourism.

scholarly journals Method for characterization of volatile organic compounds in the diet of Centris analis (Hymenoptera, Apidae, Centridini) immatures

2020 ◽  
Vol 9 (10) ◽  
pp. e5069108880
Author(s):  
Paulo Herbesson Pereira de Sousa ◽  
Cláudia Inês da Silva ◽  
Breno Magalhães Freitas ◽  
Tigressa Helena Rodrigues Soares ◽  
Isac Gabriel Abrahao Bomfim ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Volatile Compounds ◽  
Organic Compounds ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Lower Number ◽  
Deionized Water ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Organic

This study tested three types of Solid-Phase Microextraction fibers in developing a method to extract volatile organic compounds present in the diet of immature Centris analis. Samples were placed in glass vials with metal lids and added with 3g NaCl and 8 ml deionized water. Extraction and characterization were carried out using a Headspace – Solid Phase Microextraction (HS-SPME) with Gas Chromatography – Mass Spectrometry, and the three types of fibers were polydimethylsiloxane (PDMS), divinylbenzene/ carboxen/ polydimethylsiloxane (DVB/CAR/PDMS) and carboxen/ polydimethylsiloxane (CAR/PDMS). Each type of fiber was exposed to volatiles for 30 min and analyzed in a chromatograph Agilent GC-MS equipped with a quadrupole detector (MSD 5977A), containing a HP-5MS (30 m x 0.25 mm x 0.25 µm) column and Helium as the carrier gas (1 ml.min-1). The CAR / PDMS fiber favored the extraction of volatile compounds to semi-volatile compounds, followed by DVB / CAR / PDMS, while PDMS presented a lower number of extracted compounds, which can be attributed to its apolar nature. The volatile compounds identified in the diet included alcohols, aldehydes, esters, ketones, and terpenes. The SPME technique has proven effective in the extraction of volatile organic compounds from immature of Centris analis diet, being the CAR/PDMS the most suitable fiber for this.

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