scholarly journals Association analysis of SOCS3, JAK2 and STAT3 gene polymorphisms and genetic susceptibility to type 2 diabetes mellitus in Chinese population

2022 ◽  
Vol 14 (1) ◽  
Author(s):  
Yang Zhang ◽  
Chunwen Lin ◽  
Rong Chen ◽  
Ling Luo ◽  
Jialu Huang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Environmental Factors ◽  
Genetic Susceptibility ◽  
Frequency Distribution ◽  
Chinese Population ◽  
Chi Square ◽  
Chi Square Test

Abstract Aim The association of polymorphisms in the three genes of SOCS3, JAK2 and STAT3 with genetic susceptibility to type 2 diabetes mellitus (T2DM) was explored, and its interaction with environmental factors such as hypertension and triglycerides was analyzed. Methods The Hardy–Weinberg balance test was used to analyze the random balance of genes in the population. The analysis of the association of SNPs with T2DM was performed using Pearson’s chi-square test. Haplotype frequency distribution, SNPs-SNPs interaction and environmental factors were analyzed by chi-square test and logistic regression. Results The genotype distribution of SNPs rs2280148 of the SOCS3 gene was statistically significant. The allele frequency distribution of SNPs (rs4969168/rs2280148) was statistically different. After covariate correction, the SOCS3 gene locus (rs4969168) showed an association with T2DM in additive model, while the rs2280148 locus showed an association with T2DM in all three models. The locus (rs10974914/rs10815157) allele and genotype frequency distribution of JAK2 were statistically significant. After covariate correction, two SNPs in the gene showed association with T2DM in both additive and recessive models. The distribution of genotype frequencies of SNPs rs1053005 locus in gene STAT3 was statistically significant between the two groups. In recessive genetic models, rs1053005 locus polymorphisms was associated with T2DM. Haplotype S3 (G G)/S 4 (G T) of the SOCS3 gene as well as haplotype J2 (A G)/J 3 (G C) of the JAK2 gene were closely associated with T2DM. There was an interaction between SNPs rs4969168 and SNPs rs2280148 in the SOCS3 gene. There was an interaction between the SOCS3, JAK2 and STAT3 genes and hypertension/triglycerides. Conclusion The SOCS3 and JAK2 genes may be associated with T2DM in the Chinese population, in which SNPs carrying the A allele (rs4969168)/G allele (rs2280148)/C allele (rs10815157) have a reduced risk of T2DM. Haplotype S3 (G G)/S 4 (G T) of the SOCS3 gene and haplotype J2 (A G)/J 3 (G C) of the JAK2 gene may be influencing factor for T2DM. The interaction between SNPs rs4969168 and SNPs rs2280148 increases the risk of T2DM. Hypertension and triglycerides may interact with SNPs of T2DM susceptibility genes.

scholarly journals The Relationship Of The Level Of Family Knowledge And Efforts To Prevent Complications Of Diabetes Mellitus Tipe 2 In The Working Area Of Babakan Public Health Center District Of Cirebon

2021 ◽  
Vol 8 (1) ◽  
pp. 14-19
Author(s):  
Lukatul Khoiriyah ◽  
Ramli Effendi ◽  
Thia Oktiany
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Health Center ◽  
P Value ◽  
Chi Square ◽  
Work Area ◽  
Chi Square Test ◽  
The Relationship

The level of family knowledge is a very important factor in the actions of families of people with Diabetes Mellitus, behavior based on knowledge will be easier to carry out than those not based on knowledge. Diabetes Mellitus is a disease that requires proper and immediate treatment efforts because it can cause complications such as heart disease, kidney failure, and damage to the nervous system. One way to overcome the effects of Type 2 Diabetes Mellitus is by applying a diet. This study aims to determine the relationship between the level of family knowledge with efforts to prevent complications of type 2 diabetes mellitus in the work area of Babakan Puskesmas, Cirebon Regency. This research method uses descriptive correlational, this research uses cross section research. The population is all families with type 2 Diabetes Mellitus in the work area of Babakan Puskesmas in Cirebon Regency as many as 144 respondents and sampling using Purposive Sampling obtained 60 respondents. The instruments used were questionnaire sheets, and data analysis using chi-square test that is the significance value α = 0.1. The results of the above study indicate between the level of family knowledge and prevention of complications of type 2 diabetes mellitus in getting p-value = 0.04, because the p-value of 0.04 <0.1 then Ho is rejected and Ha is accepted which means there is a relationship between the level of family knowledge and prevention of complications of type 2 diabetes mellitus in the work area of Babakan Health Center, Cirebon Regency.

scholarly journals Kepatuhan dan Kualitas Hidup Pasien Diabetes Melitus Tipe 2 di Rumah Sakit di Jawa Tengah

2020 ◽  
Vol 17 (2) ◽  
pp. 165-173
Author(s):  
Nurul Mutmainah ◽  
Muhammad Al Ayubi ◽  
Anggie Widagdo
Keyword(s):  
Quality Of Life ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Short Form ◽  
Chi Square ◽  
Central Java ◽  
Chi Square Test

Diabetes mellitus is a chronic disease that requires treatment for a long period of time. Adherence to consuming drugs is one of the most important things in achieving treatment goals. This study aims to determine the level of adherence and quality of life and determine the relationship to the quality of life of patients with type 2 DM in hospitals in Central Java. This study uses analytic design by asking cross-sectional. Samples were taken at two hospitals in Central Java. Sampling was purposive sampling with the criteria of type 2 diabetes mellitus patients who are undergoing outpatient care in the hospital, have undergone therapy for at least 3 months, and can communicate well. As a measurement used the Medication Adherence Rating Scale (MARS) questionnaire while the quality of life measure used the Short Form 36 (SF-36) questionnaire. Score data received and quality of life that has been obtained was processed with the SPSS (Product Statistics and Service Solutions) chi-square test. Research results in 200 respondents found 9 people (4.5%) had low adherence, 46 people (23%) were moderately related and 145 people (72.5%) have high adherence. While the quality of life found 41 people (20.5%) had a poor quality of life and 159 people (79.5%) had a good quality of life. The lowest aspect of quality of life is physical limitations and the highest aspect of quality of life is mental health. From the results of the analysis on the chi square test, the p value was obtained: 0.00. This shows that there is a significant relationship between adherence and quality of life in patients with Type 2 diabetes mellitus at the hospitals and a Prevalence Ratio value of 23.47.

scholarly journals Association of Genetic Polymorphisms in MicroRNAs With Type 2 Diabetes Mellitus in a Chinese Population

2021 ◽  
Vol 11 ◽  
Author(s):  
Zaihan Zhu ◽  
Yanfen Zhang ◽  
Ruocen Bai ◽  
Ru Yang ◽  
Zhongyan Shan ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Environmental Factors ◽  
Signaling Pathways ◽  
Insulin Signaling ◽  
Chinese Population ◽  
Interaction Effects ◽  
Nucleotide Polymorphisms

IntroductionMicroRNAs (miRNA) involved in the insulin signaling pathways deeply affect the pathogenesis of T2DM. The aim of this study was to assess the association between single nucleotide polymorphisms (SNP) of the related miRNAs (let-7f rs10877887, let-7a-1 rs13293512, miR-133a-1 rs8089787, miR-133a-2 rs13040413, and miR-27a rs895819) and susceptibility to type 2 diabetes mellitus (T2DM), and its possible mechanisms.MethodsFive SNPs in miRNAs (let-7f rs10877887, let-7a-1 rs13293512, miR-133a-1 rs8089787, miR-133a-2 rs13040413, and miR-27a rs895819) involved in the insulin signaling pathways were selected and genotyped in a case-control study that enrolled 371 T2DM patients and 381 non-diabetic controls. The individual SNP association analyses, interaction analyses of SNP-SNP, SNP-environmental factors were performed. The effect the risk-associated polymorphism on regulating its mature miRNA expression was also evaluated.ResultsIn overall analyses, miR-133a-2 rs13040413 and let-7a-1 rs13293512 were related to the susceptibility to T2DM. In stratified analyses, miR-133a-2 rs13040413, let-7a-1 rs13293512 and miR-27a rs895819 showed associations with T2DM in the age ≥ 60 years subgroup. Moreover, let-7a-1 rs13293512 and miR-27a rs895819 showed associations with T2DM in male subgroup. In SNP-environmental factors interaction analyses, there were interaction effects of miR-133a-2 rs13040413 with dyslipidemia, let-7a-1 rs13293512 with smoking, and let-7a-1 rs13293512 with dyslipidemia on T2DM. In SNP-SNP interaction analyses, there were also interaction effects of miR-133a-1 rs8089787 with let-7a-1 rs13293512, and miR-133a-1 rs8089787 with let-7f rs10877887 on T2DM. Furthermore, for miR-133a-2 rs13040413, the variant T allele showed a trend toward decreased miR-133a expression in comparison with the wild C allele. For let-7a-1 rs13293512, the variant C allele expressed a lower let-7a compared to the wild T allele.ConclusionMiRNAs polymorphisms involved in the insulin signaling pathways and the interaction effects of SNP-SNP, SNP-environmental factors were related to T2DM susceptibility in a Chinese population.

scholarly journals HUBUNGAN POLA MAKAN TERHADAP STATUS GIZI PASIEN RAWAT JALAN DIABETES MELITUS TIPE 2 DI WILAYAH KERJA RUMAH SAKIT GRANDMED LUBUK PAKAM

2021 ◽  
Vol 3 (2) ◽  
pp. 168-171
Author(s):  
Raini Panjaitan ◽  
Reno Irwanto ◽  
Andreais Boffil Cholilullah ◽  
Salmi Angraini
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Nutrient Status ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Chi Square Test ◽  
Cross Sectional Design

Diabetes mellitus is a chronic disease characterized by hyperlycemia. Risk factors for type 2 diabetes mellitus due to intake of carbohydrates, fats and protein. This study aims to see the relationship between diet in the nutrient status of type 2 diabetes mellitus in out patient in Grandmed Hospital Lubuk Pakam. This research method is an observational description with cross-sectional design. The sample is patients type 2 diabetes mellitus in out patient in Grandmed Hospital Lubuk Pakam. The sample is 30 people. The instruments are Food Recall 24 Hours and analyzed by nutrition app Nutri Survey. The data analysis used in this study was the Chi-Square test (95% CI) From the result of this research showed that 44,6% of patients with excess carbohydrate intake with a p value of 0.000 ≤ α (0,05). this research showed that 49,0% of patients with excess fat intake with a p value of 0.000 ≤ α (0,05). this research showed that 38,0% of patients with excess protein intake with a p value of 0.000 ≤ α (0,05). The conclusion are that there is significant relationship between diet in the nutrient status of type 2 diabetes mellitus in out patient in Grandmed Hospital Lubuk Pakam and type 2 diabetes mellitus in out patient nutrient status characteristics categorized as obesity.  

scholarly journals Identification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia

2020 ◽  
Vol 9 (2) ◽  
pp. 186-191
Author(s):  
Miftahul Mushlih ◽  
Fitri Kumala Sari ◽  
Hindah Sabrina Amin ◽  
Siti Asriani Iknan
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Scoring Method ◽  
Chi Square ◽  
Cross Sectional ◽  
Chi Square Test ◽  
Random Amplified Polymorphism Dna ◽  
Rapd Method

T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.

scholarly journals Vascular Endothelial Growth Factor (VEGF) -460C/T (rs833061) Gene Polymorphism as a Risk Factor for Diabetic Retinopathy in Type 2 Diabetes Mellitus in Bali

2021 ◽  
Vol 8 (12) ◽  
pp. 1-6
Author(s):  
Astri Meliana ◽  
Desak Made Wihandani ◽  
Ni Nyoman Ayu Dewi
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diabetic Retinopathy ◽  
Risk Factor ◽  
Gene Polymorphism ◽  
Bivariate Analysis ◽  
Chi Square ◽  
Chi Square Test

Introduction: Diabetic retinopathy (DR) is a microvascular disorder of the retina caused by hyperglycemia in the blood vessels and is the most common complication in the eye due to diabetes mellitus (DM). The aim of this study was to determine the VEGF -460C/T gene polymorphism as a risk factor for diabetic retinopathy in T2DM patients in Bali. Materials and Methods: The design of this study was case-control with 27 cases of type 2 DM with DR and 29 cases without RD as controls. The VEGF-460C/T polymorphism in DNA was detected using PCR and DNA sequencing at rs833061 to see the distribution of the C/T allele variation. Data were analyzed using chi-square test. Results: Based on bivariate analysis comparing homozygous TT genotype variants, heterozygous CT and wild-type CC in this study, no significant relationship was found with the incidence of DR (p=0.742). Conclusion: Polymorphism of the VEGF-460C/T gene (rs833061) can be concluded as an irrelevant factor with the risk of developing DR in type 2 diabetes mellitus patients in Bali. Keywords: VEGF -460C/T, Diabetes Mellitus, Polymorphism, Risk Factors.

scholarly journals TYPE 2 DIABETES MELLITUS ON TRIGLYCERIDE LEVELS AND BLOOD PRESSURE

2019 ◽  
Vol 1 (1) ◽  
pp. 11-16
Author(s):  
Wirawan Anggorotomo ◽  
Tessa Sjahriani ◽  
Masroni Masroni
Keyword(s):  
Diabetes Mellitus ◽  
Blood Pressure ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Frequency Distribution ◽  
Metabolic Diseases ◽  
P Value ◽  
Chi Square ◽  
Chi Square Analysis

Background: Diabetes Mellitus (DM) is a group of metabolic diseases characterized by an abnormality of insulin secretion, insulin work, or both. DM cases in Indonesia as many as 8,5 million cases in 2013. Prevalence based on diagnosis or symptoms according to the health departement of 2,1%.Purpose: To know the correlation of type 2 diabetes mellitus on triglyceride levels and blood pressureMethods: This study used secondary data derived from the medical record status of patients with diabetes mellitus. The method used is analytic observational with cross sectional approach. Sampling technique using purposive sampling. The population of this study is the diabetes mellitus type 2 in 2017 as many as 151. Sample of 109 respondents. Data analyzed by univariate and bivariate using Chi Square analysis.Results: The frequency of respondent characteristic on female gender as much 57,8%, based on age group >45 old counted 58,7%. Blood pressure frequency distribution found hypertension as much 63,3%. High frequency distribution of triglyceride levels is 67,9%. The Chi Square analysis result there is a significant correlation of type 2 diabetes mellitus on triglyceride levels and blood pressure with Odds Ratio 6,865 with p-value of 0,009.

scholarly journals A study on diastolic dysfunction in asymptomatic type 2 diabetes mellitus with normal systolic function

2019 ◽  
Vol 6 (3) ◽  
pp. 890
Author(s):  
M. Ilamaran ◽  
A. Thangadhurai ◽  
P. Suresh Kumar ◽  
Saranya Nagalingam
Keyword(s):  
Diabetes Mellitus ◽  
Heart Failure ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diastolic Dysfunction ◽  
Diastolic Heart Failure ◽  
Chi Square ◽  
High Incidence ◽  
Chi Square Test

Background: Diastolic heart failure, otherwise called as heart failure with preserved ejection fraction, is common finding of hypertensive heart disease, but various studies report a high incidence of diastolic heart failure in patients with type 2 diabetes mellitus in spite of the absence of coronary artery heart disease and hypertension. The objectives of the study were to determine the prevalence of dysfunction of left ventricle in diastole in type 2 diabetes mellitus patients and to compare with the non-diabetic individuals with that of asymptomatic type II diabetes patients. To assess the correlation of diastolic dysfunction in diabetes with age of patients, HbA1c levels, duration of diabetes, retinopathy, autonomic neuropathy.Methods: A prospective cross sectional among them 50 patients were diabetics and 50 were non-diabetic controls. Diastolic dysfunction was measured with standard echocardiographic parameters and the results were computed with corresponding variables of the patients. All the variables and their data were analysed for percentage, mean, standard deviation ‘t’ test and chi square test. The ‘t’-test was used to study the quantitative data while chi square test was used to study the qualitative data.Results: Among the study population 60% had diastolic dysfunction and 14% had diastolic dysfunction among cases and control group respectively. Diastolic dysfunction was present among 23.3% and the 76.7% of the age group groups of less than 45 and more than 45 years of age respectively. In this study poor glycaemic status was significantly associated with diastolic dysfunction, whereas duration of diabetes, retinopathy and autonomic neuropathy were not statistically significant.Conclusions: Present study reveals moderately high incidence of diastolic dysfunction in asymptomatic diabetic; subjects and, this finding was correlated with the HBA1C levels but not with retinopathy and autonomic neuropathy.

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