scholarly journals Antimicrobial resistance profile of Staphylococcus aureus isolates isolated from ear discharges of patients at University of Hawassa comprehensive specialized hospital

2017 ◽  
Vol 18 (1) ◽  
Author(s):  
Serawit Deyno ◽  
Alemayehu Toma ◽  
Mesfin Worku ◽  
Muluken Bekele
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Resistance Profile ◽  
Specialized Hospital ◽  
Antimicrobial Resistance Profile

scholarly journals Antimicrobial Activity of the Circular Bacteriocin AS-48 against Clinical Multidrug-Resistant Staphylococcus aureus

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 925
Author(s):  
Cristina Velázquez-Suárez ◽  
Rubén Cebrián ◽  
Carmen Gasca-Capote ◽  
Antonio Sorlózano-Puerto ◽  
José Gutiérrez-Fernández ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Resistance Profile ◽  
Risk Of Death ◽  
Antimicrobial Resistance Profile ◽  
The Matrix ◽  
Division Process ◽  
Important Challenge ◽  
Clinical Source

The treatment and hospital-spread-control of methicillin-resistant Staphylococcus aureus (MRSA) is an important challenge since these bacteria are involved in a considerable number of nosocomial infections that are difficult to treat and produce prolonged hospitalization, thus also increasing the risk of death. In fact, MRSA strains are frequently resistant to all β-lactam antibiotics, and co-resistances with other drugs such as macrolides, aminoglycosides, and lincosamides are usually reported, limiting the therapeutical options. To this must be added that the ability of these bacteria to form biofilms on hospital surfaces and devices confer high antibiotic resistance and favors horizontal gene transfer of genetic-resistant mobile elements, the spreading of infections, and relapses. Here, we genotypically and phenotypically characterized 100 clinically isolated S. aureus for their resistance to 18 antibiotics (33% of them were OXA resistant MRSA) and ability to form biofilms. From them, we selected 48 strains on the basis on genotype group, antimicrobial-resistance profile, and existing OXA resistance to be assayed against bacteriocin AS-48. The results showed that AS-48 was active against all strains, regardless of their clinical source, genotype, antimicrobial resistance profile, or biofilm formation capacity, and this activity was enhanced in the presence of the antimicrobial peptide lysozyme. Finally, we explored the effect of AS-48 on formed S. aureus biofilms, observing a reduction in S. aureus S-33 viability. Changes in the matrix structure of the biofilms as well as in the cell division process were observed with scanning electron microscopy in both S-33 and S-48 S. aureus strains.

scholarly journals Antimicrobial Resistance Profile of Planktonic and Biofilm Cells of Staphylococcus aureus and Coagulase-Negative Staphylococci

2016 ◽  
Vol 17 (9) ◽  
pp. 1423 ◽  
Author(s):  
Adilson de Oliveira ◽  
Valéria Cataneli Pereira ◽  
Luiza Pinheiro ◽  
Danilo Moraes Riboli ◽  
Katheryne Benini Martins ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Coagulase Negative Staphylococci ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile

scholarly journals Change of the Frequency of Methicillin Resistance and Antibiogram Resistance Profile of Staphylococcus aureus within a Period of 15 Years

2021 ◽  
Author(s):  
Kamuran Şanlı ◽  
Selen Zeliha Mart Kömürcü ◽  
Nilgün Kansak ◽  
Rıza Adaleti
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Methicillin Resistance ◽  
Erythromycin Resistance ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile ◽  
Gentamicin Resistance ◽  
Successful Control ◽  
Mrsa Strains ◽  
Hospital Acquired

Objective: The aim of this retrospective study was to evaluate the rate and antimicrobial resistance profile of community-acquired (CA) and hospital-acquired (HA) methicillin-resistant and sensitive Staphylococcus aureus (MRSA, MSSA) strains between 2004 and 2019. Method: Within the scope of the research, the rate of MRSA and MSSA and the change in antimicrobial resistance profile over time were investigated using two research data of 210 Staphylococcus aureus strains isolated in 2004, and 401 in 2019. Results: While any significant change was not seen in the rates of CA-MRSA (32.4%) and CA-MSSA (67.6%) in 2004, and of CA-MRSA (31.6%) and CA-MSSA (68.4%) in 2019, the prevalence of HA-MRSA decreased by 56.1% in 2004 and 30.7% in 2019 and of HA-MSSA increased by 43.9% in 2004 and 69.3% in 2019. No resistance to vancomycin and teikoplanin was observed in MRSA strains. Resistance of CA-MRSA against ciprofloxacin, levofloxacin, clindamycin and gentamicin decreased. In CA-MSSA an increase of penicillin resistance as well as a decrease in gentamicin resistance was observed. In resistance of HA-MRSA against ciprofloxacin, levofloxacin, erythromycin, clindamycin, gentamicin decreased. HA-Resistance of MSSA against fusidic acid increased and against ciprofloxacin and trimethoprim/sulfamethoxazole and erythromycin resistance decreased. Conclusion: It was found that the rate of HA-MRSA decreased during the given period of 15 years. Vancomycin or teicoplanin resistance was not observed in MRSA and MSSA. While against ciprofloxacin, levofloxacin, clindamycin, gentamicin decreased in both CA-MRSA and HA-MRSA. A closer follow-up of the prevalence and antimicrobial resistance profiles of these strains is of utmost importance for the successful control of the infections caused by MRSA and MSSA.

Antimicrobial Resistance Profile and Quinolone Resistance Genes in Staphylococcus aureus from Patients Attending Federal Medical Centre Keffi, Nigeria

2020 ◽  
pp. 1-15
Author(s):  
E. A. Sunday ◽  
Y. B. Ngwai ◽  
R. H. Abimiku ◽  
I. H. Nkene ◽  
Y. Ibrahim ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Multidrug Resistant ◽  
Quinolone Resistance ◽  
Medical Centre ◽  
Clinical Samples ◽  
Resistance Profile ◽  
Antimicrobial Resistance Profile ◽  
Pcr Method

Aims: This study investigated the antimicrobial resistance profile and quinolone resistance genes in Staphylococcus aureus from patients attending Federal Medical Centre, Keffi, Nigeria. Methodology: A total of 240 clinical samples which comprised of high vaginal swabs, endocervical swabs, sputum, ear swabs, wound swabs, semen and eye swabs, were collected from the patients. Staphylococcus aureus was isolated and identified from these samples using standard microbiological method. Antimicrobial susceptibility testing of the isolates was performed and interpreted in accordance with the Clinical and Laboratory Standards Institute (CLSI) method. Ciprofloxacin-resistant S. aureus were screened for quinolone resistance genes using Polymerase Chain Reaction (PCR) method. Results: Out of 240 clinical samples, the prevalence of S. aureus was 21.3%. The prevalence in relation to clinical samples was higher in eye swab (45.5%) and ear swab (44.4%), but lower in sputum (14.5%). The isolates were more resistant to oxacillin (88.2%), sulphamethoxazole/ trimethoprim (82.4%) and erythromycin (76.5%), but less resistant to ciprofloxacin (19.6%) and levofloxacin (5.9%). The most common resistance phenotypes in the isolates were sulfamethoxazole/trimethoprim (SXT) - clindamycin (DA) – ofloxacin (OX) - erythromycin (E) - rifampicin (RD) and SXT-DA-OX-E- streptomycin (S) -RD with an occurrence of (13.7%) each. The percentage occurrences of multidrug resistant and extensive-drug resistant isolates were 92.2% and 7.8% respectively. The occurrences of quinolone resistance genes in the ciprofloxacin-resistant isolates were: aac(6′)-Ib-cr (60.0%), gyrA and gyrB (50.0%), parC (40.0%), qnrB (20.0%) and qnrS (10.0%). Conclusion: The isolates were less resistant to levofloxacin, cefoxitin, ciprofloxacin and gentamicin in the study location. Most of the ciprofloxacin-resistant isolates harbored quinolone resistance genes with aac(6′)-Ib-cr as the most common.

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