scholarly journals Antioxidant ability of Chenopodium formosanum extracted using an ethanol–ammonium sulfate two-phase system

2022 ◽  
Vol 9 (1) ◽  
Author(s):  
Wei-Hsun Wang ◽  
Wei-Lin Li ◽  
Cheng-You Chen ◽  
Min-Yun Chang ◽  
Shu-Ling Huang ◽  
...  
Keyword(s):  
Free Radical ◽  
Ammonium Sulfate ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phase System ◽  
Antioxidant Ability ◽  
Two Phase ◽  
Alcohol Extract ◽  
Dry Extract ◽  
Radical Scavenging Ability

Abstract Background Chenopodium formosanum (CF) provides the human body with numerous nutritional components. This study used the two-phase system to identify an efficient method to obtain CF extracts. CF extraction was performed using an ethanol–ammonium sulfate two-phase system. The efficacy of different CF extracts with five types of antioxidant ability was tested and compared with traditional aqueous and alcohol extractions. Results The results showed that a separated top of the two-phase system extract had higher total phenol content (120.35 ± 5.80 mg gallic acid equivalent/g dry extract), total flavonoid content (447.06 ± 16.57 mg quercetin equivalent/g dry extract) and reducing ability (284.48 ± 4.60 mg vitamin C equivalent/g dry extract) than those of other extracts. Furthermore, the separated top of the two-phase system extract and the top of the two-phase system extract had higher 1,1-diphenyl-2-picrylhydrazyl free radical scavenging ability and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radical scavenging ability than those of the water extract, alcohol extract, bottom of two-phase system extract, and separated bottom of two-phase system extract. Conclusions The results indicate that CF has great potential for use in natural plant health supplements and skin care products and that the two-phase extraction system can yield an effective CF extract. Graphical Abstract

scholarly journals Partition Behavior in Aqueous Two-Phase System and Antioxidant Activity of Flavonoids from Ginkgo biloba

2018 ◽  
Vol 8 (11) ◽  
pp. 2058 ◽  
Author(s):  
Can Liu ◽  
Shuochen Liu ◽  
Lingli Zhang ◽  
Xiaoxue Wang ◽  
Lanqing Ma
Keyword(s):  
Antioxidant Activity ◽  
Polyethylene Glycol ◽  
Free Radical ◽  
Vitamin C ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phase System ◽  
Two Phase ◽  
Extraction Parameters ◽  
Partition Behavior

In this work, the aqueous two-phase extraction parameters and in vitro antioxidant activity of total flavonoids from Ginkgo biloba were investigated. The partition behavior of the flavonoids in an aqueous two-phase system (ATPS) was analyzed, the extraction parameters were optimized using response surface methodology, and the antioxidant activity of the flavonoids was determined by free-radical scavenging tests (1,1-diphenyl-2-picrylhydrazyl (DPPH) assay) and the ferric reducing/antioxidant power (FRAP) assay. The results showed that the concentration of ammonium sulfate was negatively correlated with the phase-volume ratio, whereas the concentration of polyethylene glycol was positively correlated. The maximum yield of flavonoids (4.11 mg g−1) was obtained under the following optimal extraction conditions: Concentration of polyethylene glycol (PEG)1500 30% and (NH4)2SO4 22% (mass fraction), and liquid/solid ratio 40:1 (mL g−1). The antioxidant activity tests showed that the flavonoids from the G. biloba leaf exhibited free-radical scavenging activity, with an IC50 of 2.66 mg L−1, which was superior to that of vitamin C. The free-radical scavenging ability of the flavonoids was proportional to the flavonoid concentration. The total reducing power of the Ginkgo flavonoids was slightly lower than that of vitamin C. In this study, the distribution of flavonoids in an ATPS was analyzed and a mathematical model for the ATPS extraction of Ginkgo flavonoids was established, which provides a reference for further development and utilization of G. biloba.

Significant effects of two pesticides on the bacteriostatic activity and antioxidant ability of green tea polyphenols

2020 ◽  
Author(s):  
Jin Zhang ◽  
Meng-ting Tao ◽  
Zi-yan Huang ◽  
Gui-yun Hong ◽  
Shu-guang Zhu
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Green Tea ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Tea Polyphenols ◽  
Green Tea Polyphenols ◽  
Bacteriostatic Activity ◽  
Antioxidant Ability ◽  
Radical Scavenging Ability

Abstract Background: Green tea polyphenols (GTPs) have good bacteriostatic activity and antioxidant capacity, yet pesticide pollutants in tea may affect their functionality. This study aims to explore the effects of pesticide pollutants on the bacteriostasis and antioxidant ability of GTPs.Results: The bacteriostatic activity of GTPs and two pesticides (acetamiprid (ACE), diquat dibromide (DIQ)) shows some certain time characteristics. Two pesticides can affect the bacteriostatic activity of GTPs. The bacteriostatic activity of GTPs is enhanced or weakened by the two pesticides with time lengthening, i.e. time-dependent synergism or antagonism. The bacteriostatic mechanisms of the three substances and their mixtures is produced by affecting cell morphology or destroying cell structure, and the long-term antagonism of the three substances is may due to the competition of action site. In addition, the two pesticides can greatly reduce the antioxidant capacity of GTPs. ACE reduces the free radical scavenging ability of GTPs by 14%~24% and DIQ reduces the free radical scavenging ability of GTPs by 39%~63% at the experimental concentration ratios.Conclusions: Two pesticides has significant effects on the bacteriostatic activity and antioxidant ability of GTPs.

scholarly journals Research antioxidant activity of chitooligosacchride by UV-VIS absorption spectrocopy

2020 ◽  
Vol 8 (1) ◽  
pp. 75-81
Author(s):  
Bui Van Hoai ◽  
Ngo Dai Nghiep ◽  
Dao An Quang ◽  
Nguyen Thi Nam Phuong
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Absorption Spectrum ◽  
Free Radical ◽  
Trichoderma Viride ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Degree Of Deacetylation ◽  
Radical Scavenging Ability

Chitosan with 80% degree of deacetylation was hydrolyzed by cellulase of Trichoderma viride to prepare chitooligosaccharides (COSs) by the fractionation of the COSs with ultrafiltration membrane. The antioxidant activities of the COSs were clarified in this study by reducing power and free radical scavenging ability assay by UV-VIS absorption spectrum. The results show that the COS 1 (10,000-5,000 Da), COS 2 (5,000-3,000 Da), COS 3 (3,000-1,000 Da) and COS 4 (less than1,000 Da) segments have antioxidant properties.The antioxidant activitives of the COSs increased with the increment of concentration, and they also depended on molecular weight.

Potential hepatoprotective effects of fullerenol nanoparticles on alcohol-induced oxidative stress by ROS

RSC Advances ◽  
2016 ◽  
Vol 6 (37) ◽  
pp. 31122-31130 ◽  
Author(s):  
Y. Li ◽  
H. B. Luo ◽  
H. Y. Zhang ◽  
Q. Guo ◽  
H. C. Yao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hepatoprotective Effects ◽  
Radical Scavenging Ability

The free radical scavenging ability of fullerenols is their most exploited property in biomedical studies.

scholarly journals Construction of poly(dopamine) doped oligopeptide hydrogel

RSC Advances ◽  
2017 ◽  
Vol 7 (80) ◽  
pp. 50425-50429 ◽  
Author(s):  
Chen-Ming Zhang ◽  
Si-Yong Qin ◽  
Yin-Jia Cheng ◽  
Ai-Qing Zhang
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Ability

Incorporation of poly(dopamine) (PDA) into the native oligopeptide hydrogel not only improve the rigidity but also endow the hydrogel with efficient free radical scavenging ability.

Effects of Cooking Methods and Tea Marinades on the Formation of Heterocyclic Amines and Benzo[a]pyrene in Grilled Drumsticks

2020 ◽  
Vol 83 (2) ◽  
pp. 365-376
Author(s):  
MINGJUN YAO ◽  
IFTIKHAR ALI KHAN ◽  
YIQUN CHENG ◽  
YUN ANG ◽  
XINGHU ZHOU ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Content ◽  
Free Radical Scavenging ◽  
Heterocyclic Amines ◽  
Cooking Methods ◽  
Scavenging Activity ◽  
Radical Scavenging Ability

ABSTRACT The effects of different grilling methods and tea marinades on the formation of heterocyclic amines (HCAs) and benzo[a]pyrene (BaP) in grilled chicken drumsticks were investigated. This study showed that both the grilling method and type of charcoal used in charcoal grilling had a significant effect on the formation of HCAs and BaP. The total content of HCAs and BaP detected in drumsticks was the lowest under electric roasting, at 7.25 and 0.04 ng/g, respectively; thus, the electric grill can be used as an alternative grilling method to meet consumer demands for safety. The free radical scavenging ability of tea infusion was significantly higher than that of spice infusion. The white tea marinade significantly increased radical scavenging activity and prevented formation of HCAs and BaP more than the green tea marinade; thus, tea marinade can be applied to flavoring as a pretreatment to preserve the quality of meat and to decrease the generation of HCAs and BaP during cooking. HIGHLIGHTS

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